LOCUS       sequence-450260-       11067 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses exogenous GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11067)
  AUTHORS   Muthukumar G, Stevens TA, Inglis AJ, Esantsi TK, Saunders RA, 
            Schulte F, Voorhees RM, Guna A, Weissman JS
  TITLE     Triaging of alpha-helical proteins to the mitochondrial outer 
            membrane by distinct chaperone machinery based on substrate 
            topology.
  JOURNAL   Mol Cell. 2024 Mar 21;84(6):1101-1119.e9. doi: 
            10.1016/j.molcel.2024.01.028. Epub 2024 Feb 29.
  PUBMED    38428433
REFERENCE   2  (bases 1 to 11067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.01.028"; journalName: "Mol Cell"; date: 
            "2024-03-21- 21"; volume: "84"; issue: "6"; pages: "1101-1119.e9"
COMMENT     Sequence Label: pAG66
FEATURES             Location/Qualifiers
     source          1..11067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    678..803
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2047..2164
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2896..4073
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3127..4065
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4022..4042
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(4122..4124,4125..4835)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             4122..4835
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4152..4180)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4775..4795
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    4928..5516
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4981..5001)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5399..5410)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5519..5535)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5520..5536)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5626..5859
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5884..5902)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5885..5902)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6192..6211)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6425..6444)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6544..6566
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6604..6622)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6690..6794
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6795..6863,6864..7655)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7013..7032)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7826..8414
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8315..8334
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8568..8585
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8655..8675)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8660..8989
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8840..8975
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10123..10188
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10318..10338
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10763..10897
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10800..10819)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10854..10873
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11065..11067,1..631)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
       61 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
      121 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
      181 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
      241 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
      301 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
      361 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
      421 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
      481 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      541 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      601 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
      661 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      721 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
      781 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
      841 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
      901 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
      961 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1021 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1081 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1141 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgccaaatgg cagtattcat
     2041 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2101 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     2161 ttttcgggtt tattacaggg acagcagaga tccagtttgg atcgataagc ttgatatcga
     2221 attcgggagg tggtccctgc agttacgcca atgataaccc ccgccagaaa aatcttagta
     2281 gccttccctt tttgttttcc gtgccccaac tcggcggatt gactcggccc cttccggaaa
     2341 cacccgaatc aacttctagt caaattattg ttcacgccgc aatgacccac ccctggcccg
     2401 cgtctgtgga actgacccct ggtgtacagg agagttcgct gctgaaagtg gtcccaaagg
     2461 ggtactagtt tttaagctcc caactccccc tcccccagcg tctggaggat tccacaccct
     2521 cgcaccgcag gggcgaggaa gtgggcggag tccggttttg gcgccagccg ctgaggctgc
     2581 caagcagaaa agccaccgct gaggagactc cggtcactgt cctcgccccg cctccccctt
     2641 ccctcccctt ggggaccacc gggcgccacg ccgcgaacgg taagtgccgc ggtcgtcggc
     2701 gcctccgccc tccccctagg gccccaattc ccagcgggcg cggcgcgcgg cccctccccc
     2761 cgccgggcgc gcgcccgctg ccccgccctt cgtggccgcc cggcgtgggc ggtgccaccc
     2821 ctccccccgg cggccccgcg cgcagctccc ggctccctcc cccttcggat gtggcttgag
     2881 ctgtaggcgc ggaggggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     2941 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
     3001 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     3061 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     3121 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     3181 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
     3241 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
     3301 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     3361 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     3421 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     3481 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     3541 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     3601 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     3661 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     3721 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     3781 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     3841 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     3901 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     3961 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     4021 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtggaattct
     4081 cagatatcaa gcttgcggta cgcgggcccg ggatcgccac catgagcaaa ggagaagaac
     4141 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
     4201 tttctgtccg tggagagggt gaaggtgatg ctacaaacgg aaaactcacc cttaaattta
     4261 tttgcactac tggaaaacta cctgttccgt ggccaacact tgtcactact ctgacctatg
     4321 gtgttcaatg cttttcccgt tatccggatc acatgaaacg gcatgacttt ttcaagagtg
     4381 ccatgcccga aggttatgta caggaacgca ctatatcttt caaagatgac gggacctaca
     4441 agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt taatcgtatc gagttaaagg
     4501 gtattgattt taaagaagat ggaaacattc ttggacacaa actagagtac aactttaact
     4561 cacacaatgt atacatcacg gcagacaaac aaaagaatgg aatcaaagct aacttcaaaa
     4621 ttcgccacaa cgttgaagat ggttccgttc aactagcaga ccattatcaa caaaatactc
     4681 caattggcga tggccctgtc cttttaccag acaaccatta cctgtcgaca caatctgtcc
     4741 tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct tcttgagttt gtaactgctg
     4801 ctgggattac acatggcatg gatgagctct acaaaggatc cggcgcgccg gatcctcgag
     4861 tctagagggc ggccgcgact ctagagtcga cctgcaggca tgcaagcttg atatcaagct
     4921 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     4981 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     5041 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     5101 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     5161 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     5221 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     5281 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     5341 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     5401 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     5461 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     5521 ataccgtcga cctcgaggga attaattcga gctcggtacc tttaagacca atgacttaca
     5581 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     5641 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     5701 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     5761 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     5821 ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg
     5881 tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta
     5941 gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca
     6001 gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg
     6061 agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat
     6121 cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
     6181 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
     6241 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
     6301 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
     6361 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc
     6421 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     6481 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     6541 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     6601 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     6661 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6721 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6781 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6841 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6901 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6961 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7021 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7081 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7141 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7201 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7261 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7321 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7381 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7441 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7501 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7561 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7621 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7681 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7741 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7801 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7861 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7921 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7981 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8041 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8101 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8161 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8221 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8281 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8341 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8401 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8461 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8521 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8581 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     8641 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     8701 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     8761 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     8821 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     8881 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     8941 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
     9001 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
     9061 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
     9121 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
     9181 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
     9241 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
     9301 gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt
     9361 ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga
     9421 aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt
     9481 tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg
     9541 tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc
     9601 gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct
     9661 ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta
     9721 ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct
     9781 ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg
     9841 tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga
     9901 tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga
     9961 tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg
    10021 gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
    10081 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
    10141 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
    10201 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
    10261 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
    10321 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
    10381 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
    10441 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
    10501 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
    10561 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
    10621 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
    10681 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    10741 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
    10801 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    10861 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    10921 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    10981 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    11041 aatatgtact acaaacttag tagttgg
//
