LOCUS       sequence-450261-       12016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses exogenous MTCH2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12016)
  AUTHORS   Guna A, Stevens TA, Inglis AJ, Replogle JM, Esantsi TK, Muthukumar 
            G, Shaffer KCL, Wang ML, Pogson AN, Jones JJ, Lomenick B, Chou TF, 
            Weissman JS, Voorhees RM
  TITLE     MTCH2 is a mitochondrial outer membrane protein insertase.
  JOURNAL   Science. 2022 Oct 21;378(6617):317-322. doi: 
            10.1126/science.add1856. Epub 2022 Oct 20.
  PUBMED    36264797
REFERENCE   2  (bases 1 to 12016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.add1856"; journalName: "Science"; date: 
            "2022-10-21- 21"; volume: "378"; issue: "6617"; pages: "317-322"
COMMENT     Sequence Label: pAG496
FEATURES             Location/Qualifiers
     source          1..12016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    678..803
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2047..2164
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2896..4073
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3127..4065
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4022..4042
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4137..4835
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4845..4901
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    5877..6465
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5930..5950)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6348..6359)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6468..6484)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6469..6485)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6575..6808
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6833..6851)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6834..6851)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7141..7160)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7374..7393)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7493..7515
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7553..7571)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7639..7743
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7744..7812,7813..8604)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7962..7981)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8775..9363
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9264..9283
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9517..9534
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9604..9624)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9609..9938
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9789..9924
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11072..11137
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11267..11287
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11712..11846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11749..11768)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11803..11822
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(12014..12016,1..631)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
       61 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
      121 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
      181 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
      241 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
      301 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
      361 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
      421 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
      481 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      541 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      601 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
      661 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      721 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
      781 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
      841 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
      901 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
      961 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1021 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1081 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1141 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgccaaatgg cagtattcat
     2041 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2101 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     2161 ttttcgggtt tattacaggg acagcagaga tccagtttgg atcgataagc ttgatatcga
     2221 attcgggagg tggtccctgc agttacgcca atgataaccc ccgccagaaa aatcttagta
     2281 gccttccctt tttgttttcc gtgccccaac tcggcggatt gactcggccc cttccggaaa
     2341 cacccgaatc aacttctagt caaattattg ttcacgccgc aatgacccac ccctggcccg
     2401 cgtctgtgga actgacccct ggtgtacagg agagttcgct gctgaaagtg gtcccaaagg
     2461 ggtactagtt tttaagctcc caactccccc tcccccagcg tctggaggat tccacaccct
     2521 cgcaccgcag gggcgaggaa gtgggcggag tccggttttg gcgccagccg ctgaggctgc
     2581 caagcagaaa agccaccgct gaggagactc cggtcactgt cctcgccccg cctccccctt
     2641 ccctcccctt ggggaccacc gggcgccacg ccgcgaacgg taagtgccgc ggtcgtcggc
     2701 gcctccgccc tccccctagg gccccaattc ccagcgggcg cggcgcgcgg cccctccccc
     2761 cgccgggcgc gcgcccgctg ccccgccctt cgtggccgcc cggcgtgggc ggtgccaccc
     2821 ctccccccgg cggccccgcg cgcagctccc ggctccctcc cccttcggat gtggcttgag
     2881 ctgtaggcgc ggaggggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     2941 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
     3001 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     3061 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     3121 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     3181 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
     3241 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
     3301 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     3361 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     3421 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     3481 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     3541 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     3601 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     3661 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     3721 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     3781 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     3841 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     3901 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     3961 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     4021 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtggaattct
     4081 cacgcgtctg caggatatca agcttgcggt accgcgggcc cgaagcttac gccaccatga
     4141 gcgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc
     4201 atcacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga
     4261 gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct
     4321 tcctctacgg cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc
     4381 agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc
     4441 tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca
     4501 gaggggtgaa cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg
     4561 ccttcaccga gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc
     4621 tgaagctcgt gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga
     4681 aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa
     4741 tcaaggaggc caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact
     4801 gcgacctccc tagcaaactg gggcacaagc ttaatggaag cggagctact aacttcagcc
     4861 tgctgaagca ggctggagac gtggaggaga accctggacc tggatccgcg gacgcggcca
     4921 gtcaggtgct cctgggctcc ggtctcacca tcctgtccca gccgctcatg tacgtgaaag
     4981 tgctcatcca ggtgggatat gagcctcttc ctccaacaat aggacgaaat atttttgggc
     5041 ggcaagtgtg tcagcttcct ggtctcttta gttatgctca gcacattgcc agtatcgatg
     5101 ggaggcgcgg gttgttcaca ggcttaactc caagactgtg ttcgggagtc cttggaactg
     5161 tggtccatgg taaagtttta cagcattacc aggagagtga caagggtgag gagttaggac
     5221 ctggaaatgt acagaaagaa gtctcatctt cctttgacca cgttatcaag gagacaactc
     5281 gagagatgat cgctcgttct gctgctaccc tcatcacaca tcccttccat gtgatcactc
     5341 tgagatctat ggtacagttc attggcagag aatccaagta ctgtggactt tgtgattcca
     5401 taataaccat ctatcgggaa gagggcattc taggattttt cgcgggtctt gttcctcgcc
     5461 ttctaggtga catcctttct ttgtggctgt gtaactcact ggcctacctc gtcaatacct
     5521 atgcactgga cagtggggtt tctaccatga atgaaatgaa gagttattct caagctgtca
     5581 caggattttt tgcgagtatg ttgacctatc cctttgtgct tgtctccaat cttatggctg
     5641 tcaacaactg tggtcttgct ggtggatgcc ctccttactc cccaatatat acgtcttgga
     5701 tagactgttg gtgcatgcta caaaaagagg ggaatatgag ccgaggaaat agcttatttt
     5761 tccggaaggt cccctttggg aagacttatt gttgtgacct gaaaatgtta atttaataag
     5821 gccgcgactc tagagtcgac ctgcaggcat gcaagcttga tatcaagctt atcgataatc
     5881 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5941 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6001 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6061 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6121 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6181 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6241 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     6301 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6361 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     6421 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     6481 ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
     6541 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     6601 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     6661 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6721 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6781 ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt
     6841 gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
     6901 acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat
     6961 ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta
     7021 acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga
     7081 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
     7141 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
     7201 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
     7261 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
     7321 gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc
     7381 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     7441 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     7501 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     7561 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     7621 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7681 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7741 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7801 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7861 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7921 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7981 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     8041 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     8101 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     8161 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     8221 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8281 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8341 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8401 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8461 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8521 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8581 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8641 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8701 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8761 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8821 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8881 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     8941 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     9001 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     9061 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     9121 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9181 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     9241 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9301 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9361 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     9421 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     9481 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9541 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     9601 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     9661 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     9721 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     9781 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     9841 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     9901 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct
     9961 tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
    10021 gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
    10081 cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
    10141 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
    10201 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac
    10261 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
    10321 gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg
    10381 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
    10441 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
    10501 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
    10561 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
    10621 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
    10681 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
    10741 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
    10801 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
    10861 cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc
    10921 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt
    10981 tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
    11041 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
    11101 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
    11161 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
    11221 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
    11281 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
    11341 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
    11401 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
    11461 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
    11521 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
    11581 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
    11641 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
    11701 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    11761 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    11821 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    11881 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
    11941 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    12001 caaacttagt agttgg
//
