LOCUS       sequence-450268-       11645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a reporter for an alpha-helical OMM protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11645)
  AUTHORS   Guna A, Stevens TA, Inglis AJ, Replogle JM, Esantsi TK, Muthukumar 
            G, Shaffer KCL, Wang ML, Pogson AN, Jones JJ, Lomenick B, Chou TF, 
            Weissman JS, Voorhees RM
  TITLE     MTCH2 is a mitochondrial outer membrane protein insertase.
  JOURNAL   Science. 2022 Oct 21;378(6617):317-322. doi: 
            10.1126/science.add1856. Epub 2022 Oct 20.
  PUBMED    36264797
REFERENCE   2  (bases 1 to 11645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.add1856"; journalName: "Science"; date: 
            "2022-10-21- 21"; volume: "378"; issue: "6617"; pages: "317-322"
COMMENT     Sequence Label: pAG517
FEATURES             Location/Qualifiers
     source          1..11645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    678..803
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2047..2164
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2896..4073
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3127..4065
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4022..4042
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4796..4852
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5375..5422
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     misc_feature    5506..6094
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5559..5579)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5977..5988)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6097..6113)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6098..6114)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6204..6437
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6462..6480)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6463..6480)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6770..6789)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7003..7022)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7122..7144
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7182..7200)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7268..7372
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7373..7441,7442..8233)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7591..7610)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8404..8992
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8893..8912
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9146..9163
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9233..9253)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9238..9567
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9418..9553
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10701..10766
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10896..10916
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11341..11475
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11378..11397)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11432..11451
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11643..11645,1..631)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
       61 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
      121 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
      181 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
      241 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
      301 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
      361 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
      421 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
      481 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      541 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      601 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
      661 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      721 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
      781 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
      841 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
      901 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
      961 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1021 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1081 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1141 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgccaaatgg cagtattcat
     2041 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2101 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     2161 ttttcgggtt tattacaggg acagcagaga tccagtttgg atcgataagc ttgatatcga
     2221 attcgggagg tggtccctgc agttacgcca atgataaccc ccgccagaaa aatcttagta
     2281 gccttccctt tttgttttcc gtgccccaac tcggcggatt gactcggccc cttccggaaa
     2341 cacccgaatc aacttctagt caaattattg ttcacgccgc aatgacccac ccctggcccg
     2401 cgtctgtgga actgacccct ggtgtacagg agagttcgct gctgaaagtg gtcccaaagg
     2461 ggtactagtt tttaagctcc caactccccc tcccccagcg tctggaggat tccacaccct
     2521 cgcaccgcag gggcgaggaa gtgggcggag tccggttttg gcgccagccg ctgaggctgc
     2581 caagcagaaa agccaccgct gaggagactc cggtcactgt cctcgccccg cctccccctt
     2641 ccctcccctt ggggaccacc gggcgccacg ccgcgaacgg taagtgccgc ggtcgtcggc
     2701 gcctccgccc tccccctagg gccccaattc ccagcgggcg cggcgcgcgg cccctccccc
     2761 cgccgggcgc gcgcccgctg ccccgccctt cgtggccgcc cggcgtgggc ggtgccaccc
     2821 ctccccccgg cggccccgcg cgcagctccc ggctccctcc cccttcggat gtggcttgag
     2881 ctgtaggcgc ggaggggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     2941 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
     3001 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     3061 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     3121 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     3181 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
     3241 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
     3301 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     3361 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     3421 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     3481 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     3541 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     3601 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     3661 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     3721 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     3781 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     3841 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     3901 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     3961 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     4021 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtggaattct
     4081 cacgcgtgac tctagcgttt aaacttaagc ttacggtacc atgaacatgg cgattatcaa
     4141 ggaattcatg aggttcaaag ttcacatgga gggatctgta aacggccacg agttcgaaat
     4201 cgagggagag ggggagggtc gaccatatga aggaacacag accgctaaat tgaaggttac
     4261 taaaggtgga ccacttcctt tcgcatggga cattcttagc ccccagttta tgtatggttc
     4321 caaggcgtat gtcaaacatc ccgcagacat tcctgattac cttaaactga gttttccaga
     4381 ggggttcaaa tgggagcgcg tcatgaactt tgaagatggg ggcgttgtta cagtgactca
     4441 ggactcttct ttgcaagatg gagagtttat atataaggtg aaacttcgcg gaaccaactt
     4501 cccaagcgat ggcccagtca tgcagaagaa aactatgggt tgggaagcca gtagcgagcg
     4561 gatgtaccca gaggacggcg cacttaaggg tgagattaag cagcggctga aactcaaaga
     4621 cggaggccat tatgacgcgg aagttaaaac gacgtacaaa gctaaaaagc ctgtccaatt
     4681 gccaggcgcg tacaatgtca atattaaatt ggacattacc agtcacaacg aagattacac
     4741 aatagtggag caatacgaac gcgcagaggg acgccattca acagggggaa gcggagctac
     4801 taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac ctggatcctc
     4861 tcagagcaac cgggagctgg tggttgactt tctctcctac aagctttccc agaaaggata
     4921 cagctggagt cagtttagtg atgtggaaga gaacaggact gaggccccag aagggactga
     4981 atcggagatg gagaccccca gtgccatcaa tggcaaccca tcctggcacc tggcagacag
     5041 ccccgcggtg aatggagcca ctggccacag cagcagtttg gatgcccggg aggtgatccc
     5101 catggcagca gtaaagcaag cgctgaggga ggcaggcgac gagtttgaac tgcggtaccg
     5161 gcgggcattc agtgacctga catcccagct ccacatcacc ccagggacag catatcagag
     5221 ctttgaacag gatacttttg tggaactcta tgggaacaat gcagcagccg agagccgaaa
     5281 gggccaggaa cgcttcaacc gctggttcct gacgggcatg actgtggccg gcgtggttct
     5341 gctgggctca ctcttcagtc ggaaaggatc cggacgtgac cacatggtcc ttcatgagta
     5401 tgtaaatgct gctgggatta cataactcga gtctagaggg cccgtttaaa cccgctgatc
     5461 agcctcgacc tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat
     5521 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5581 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5641 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5701 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5761 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5821 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5881 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     5941 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6001 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6061 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagggaat
     6121 taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     6181 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     6241 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6301 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6361 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6421 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat
     6481 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
     6541 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
     6601 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
     6661 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
     6721 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     6781 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     6841 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     6901 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     6961 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc
     7021 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     7081 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     7141 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     7201 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     7261 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7321 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     7381 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     7441 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     7501 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     7561 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     7621 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7681 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7741 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     7801 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7861 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     7921 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     7981 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8041 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8101 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8161 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8221 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8281 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8341 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8401 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8461 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     8521 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     8581 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8641 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8701 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8761 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8821 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8881 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     8941 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9001 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9061 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     9121 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     9181 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg
     9241 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9301 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     9361 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     9421 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     9481 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9541 ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg
     9601 tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc
     9661 atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc
     9721 ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata
     9781 catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc
     9841 ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta
     9901 ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac
     9961 gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc
    10021 gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca
    10081 aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat
    10141 gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc
    10201 gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt
    10261 tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac
    10321 acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac
    10381 gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa
    10441 aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga
    10501 cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga
    10561 cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc
    10621 cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
    10681 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
    10741 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
    10801 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
    10861 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
    10921 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
    10981 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
    11041 aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca
    11101 tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa
    11161 aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata
    11221 gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta
    11281 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
    11341 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    11401 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    11461 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
    11521 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
    11581 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    11641 gttgg
//
