LOCUS       sequence-450269-       11429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a reporter for an alpha-helical OMM protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11429)
  AUTHORS   Muthukumar G, Stevens TA, Inglis AJ, Esantsi TK, Saunders RA, 
            Schulte F, Voorhees RM, Guna A, Weissman JS
  TITLE     Triaging of alpha-helical proteins to the mitochondrial outer 
            membrane by distinct chaperone machinery based on substrate 
            topology.
  JOURNAL   Mol Cell. 2024 Mar 21;84(6):1101-1119.e9. doi: 
            10.1016/j.molcel.2024.01.028. Epub 2024 Feb 29.
  PUBMED    38428433
REFERENCE   2  (bases 1 to 11429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.01.028"; journalName: "Mol Cell"; date: 
            "2024-03-21- 21"; volume: "84"; issue: "6"; pages: "1101-1119.e9"
COMMENT     Sequence Label: pAG207
FEATURES             Location/Qualifiers
     source          1..11429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    678..803
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2047..2164
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2896..4073
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3127..4065
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4022..4042
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4796..4852
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5159..5206
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     misc_feature    5290..5878
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5343..5363)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5761..5772)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5881..5897)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5882..5898)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5988..6221
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6246..6264)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6247..6264)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6554..6573)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6787..6806)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6906..6928
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6966..6984)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7052..7156
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7157..7225,7226..8017)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7375..7394)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8188..8776
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8677..8696
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8930..8947
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9017..9037)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9022..9351
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9202..9337
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10485..10550
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10680..10700
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11125..11259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11162..11181)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11216..11235
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11427..11429,1..631)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
       61 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
      121 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
      181 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
      241 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
      301 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
      361 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
      421 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
      481 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      541 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      601 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
      661 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      721 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
      781 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
      841 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
      901 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
      961 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1021 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1081 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1141 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgccaaatgg cagtattcat
     2041 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2101 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     2161 ttttcgggtt tattacaggg acagcagaga tccagtttgg atcgataagc ttgatatcga
     2221 attcgggagg tggtccctgc agttacgcca atgataaccc ccgccagaaa aatcttagta
     2281 gccttccctt tttgttttcc gtgccccaac tcggcggatt gactcggccc cttccggaaa
     2341 cacccgaatc aacttctagt caaattattg ttcacgccgc aatgacccac ccctggcccg
     2401 cgtctgtgga actgacccct ggtgtacagg agagttcgct gctgaaagtg gtcccaaagg
     2461 ggtactagtt tttaagctcc caactccccc tcccccagcg tctggaggat tccacaccct
     2521 cgcaccgcag gggcgaggaa gtgggcggag tccggttttg gcgccagccg ctgaggctgc
     2581 caagcagaaa agccaccgct gaggagactc cggtcactgt cctcgccccg cctccccctt
     2641 ccctcccctt ggggaccacc gggcgccacg ccgcgaacgg taagtgccgc ggtcgtcggc
     2701 gcctccgccc tccccctagg gccccaattc ccagcgggcg cggcgcgcgg cccctccccc
     2761 cgccgggcgc gcgcccgctg ccccgccctt cgtggccgcc cggcgtgggc ggtgccaccc
     2821 ctccccccgg cggccccgcg cgcagctccc ggctccctcc cccttcggat gtggcttgag
     2881 ctgtaggcgc ggaggggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     2941 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
     3001 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     3061 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     3121 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     3181 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
     3241 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
     3301 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     3361 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     3421 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     3481 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     3541 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     3601 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     3661 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     3721 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     3781 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     3841 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     3901 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     3961 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     4021 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtggaattct
     4081 cacgcgtgac tctagcgttt aaacttaagc ttacggtacc atgaacatgg cgattatcaa
     4141 ggaattcatg aggttcaaag ttcacatgga gggatctgta aacggccacg agttcgaaat
     4201 cgagggagag ggggagggtc gaccatatga aggaacacag accgctaaat tgaaggttac
     4261 taaaggtgga ccacttcctt tcgcatggga cattcttagc ccccagttta tgtatggttc
     4321 caaggcgtat gtcaaacatc ccgcagacat tcctgattac cttaaactga gttttccaga
     4381 ggggttcaaa tgggagcgcg tcatgaactt tgaagatggg ggcgttgtta cagtgactca
     4441 ggactcttct ttgcaagatg gagagtttat atataaggtg aaacttcgcg gaaccaactt
     4501 cccaagcgat ggcccagtca tgcagaagaa aactatgggt tgggaagcca gtagcgagcg
     4561 gatgtaccca gaggacggcg cacttaaggg tgagattaag cagcggctga aactcaaaga
     4621 cggaggccat tatgacgcgg aagttaaaac gacgtacaaa gctaaaaagc ctgtccaatt
     4681 gccaggcgcg tacaatgtca atattaaatt ggacattacc agtcacaacg aagattacac
     4741 aatagtggag caatacgaac gcgcagaggg acgccattca acagggggaa gcggagctac
     4801 taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac ctggatccat
     4861 ggatcctctc agggcccaac agctggctgc ggagctggag gtggagatga tggccgatat
     4921 gtacaacaga atgaccagtg cctgccaccg gaagtgtgtg cctcctcact acaaggaagc
     4981 agagctctcc aagggcgagt ctgtgtgcct ggaccgatgt gtctctaagt acctggacat
     5041 ccatgagcgg atgggcaaaa agttgacaga gttgtctatg caggatgaag agctgatgaa
     5101 gagggtgcag cagagctctg ggcctgcagg atccgggtcc ggctcaggat ccggatcccg
     5161 tgaccacatg gtccttcatg agtatgtaaa tgctgctggg attacataac tcgagtctag
     5221 agggcccgtt taaacccgct gatcagcctc gacctgcagg catgcaagct tgatatcaag
     5281 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5341 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5401 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5461 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5521 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5581 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5641 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     5701 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5761 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     5821 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     5881 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
     5941 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     6001 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     6061 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6121 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6181 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
     6241 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
     6301 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
     6361 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
     6421 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
     6481 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     6541 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     6601 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     6661 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     6721 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
     6781 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     6841 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     6901 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     6961 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     7021 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7081 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7141 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7201 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7261 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7321 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7381 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7441 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7501 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7561 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7621 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7681 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7741 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7801 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7861 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7921 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7981 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8041 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8101 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8161 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8221 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8281 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8341 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8401 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8461 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8521 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8581 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8641 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8701 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8761 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8821 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8881 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8941 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     9001 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     9061 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     9121 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     9181 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     9241 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     9301 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga
     9361 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg
     9421 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
     9481 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
     9541 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
     9601 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     9661 tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
     9721 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
     9781 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg
     9841 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
     9901 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag
     9961 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
    10021 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag
    10081 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
    10141 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc
    10201 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
    10261 gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt
    10321 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg
    10381 cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg
    10441 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
    10501 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
    10561 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
    10621 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
    10681 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
    10741 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
    10801 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
    10861 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
    10921 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
    10981 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
    11041 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
    11101 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
    11161 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    11221 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
    11281 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
    11341 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
    11401 taaatatgta ctacaaactt agtagttgg
//
