LOCUS       sequence-45027-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Sheng Y, Laister RC, Lemak A, Wu B, Tai E, Duan S, Lukin J, 
            Sunnerhagen M, Srisailam S, Karra M, Benchimol S, Arrowsmith CH
  TITLE     Molecular basis of Pirh2-mediated p53 ubiquitylation.
  JOURNAL   Nat Struct Mol Biol. 2008 Dec . 15(12):1334-42.
  PUBMED    19043414
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2008 Dec . 15(12):1334-42."
COMMENT     Sequence Label: p53-(1-393)-E285K-N268D
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..72
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..99
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tccctctaga atattttgtt tactttaaga aggagatata ccatgggcag cagccatcat
       61 catcatcatc acagcagcgg cctggtgccg cgcggcagcc atatggagga gccgcagtca
      121 gatcctagcg tcgagccccc tctgagtcag gaaacatttt cagacctatg gaaactactt
      181 cctgaaaaca acgttctgtc ccccttgccg tcccaagcaa tggatgattt gatgctgtcc
      241 ccggacgata ttgaacaatg gttcaccgaa gacccaggtc cagatgaagc tcccagaatg
      301 ccagaggctg ctccccccgt ggcccctgca ccagcagctc ctacaccggc ggcccctgca
      361 ccagccccct cctggcccct gtcatcttct gtcccttccc agaaaaccta ccagggcagc
      421 tacggtttcc gtctgggctt cttgcattct gggacagcca agtctgtgac ttgcacgtac
      481 tcccctgccc tcaacaagat gttttgccaa ctggccaaga cctgccctgt gcagctgtgg
      541 gttgattcca cacccccgcc cggcacccgc gtccgcgcca tggccatcta caagcagtca
      601 cagcacatga cggaggttgt gaggcgctgc ccccaccatg agcgctgctc agatagcgat
      661 ggtctggccc ctcctcagca tcttatccga gtggaaggaa atttgcgtgt ggagtatttg
      721 gatgacagaa acacttttcg acatagtgtg gtggtgccct atgagccgcc tgaggttggc
//
