LOCUS       sequence-450270-       11570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a reporter for an alpha-helical OMM protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11570)
  AUTHORS   Guna A, Stevens TA, Inglis AJ, Replogle JM, Esantsi TK, Muthukumar 
            G, Shaffer KCL, Wang ML, Pogson AN, Jones JJ, Lomenick B, Chou TF, 
            Weissman JS, Voorhees RM
  TITLE     MTCH2 is a mitochondrial outer membrane protein insertase.
  JOURNAL   Science. 2022 Oct 21;378(6617):317-322. doi: 
            10.1126/science.add1856. Epub 2022 Oct 20.
  PUBMED    36264797
REFERENCE   2  (bases 1 to 11570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.add1856"; journalName: "Science"; date: 
            "2022-10-21- 21"; volume: "378"; issue: "6617"; pages: "317-322"
COMMENT     Sequence Label: pAG397
FEATURES             Location/Qualifiers
     source          1..11570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    678..803
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2047..2164
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2896..4073
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3127..4065
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4022..4042
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4796..4852
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5300..5347
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     misc_feature    5431..6019
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5484..5504)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5902..5913)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6022..6038)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6023..6039)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6129..6362
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6387..6405)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6388..6405)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6695..6714)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6928..6947)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7047..7069
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7107..7125)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7193..7297
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7298..7366,7367..8158)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7516..7535)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8329..8917
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8818..8837
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9071..9088
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9158..9178)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9163..9492
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9343..9478
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10626..10691
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10821..10841
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11266..11400
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11303..11322)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11357..11376
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11568..11570,1..631)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
       61 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
      121 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
      181 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
      241 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
      301 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
      361 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
      421 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
      481 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      541 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      601 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
      661 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      721 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
      781 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
      841 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
      901 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
      961 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1021 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1081 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1141 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgccaaatgg cagtattcat
     2041 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2101 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     2161 ttttcgggtt tattacaggg acagcagaga tccagtttgg atcgataagc ttgatatcga
     2221 attcgggagg tggtccctgc agttacgcca atgataaccc ccgccagaaa aatcttagta
     2281 gccttccctt tttgttttcc gtgccccaac tcggcggatt gactcggccc cttccggaaa
     2341 cacccgaatc aacttctagt caaattattg ttcacgccgc aatgacccac ccctggcccg
     2401 cgtctgtgga actgacccct ggtgtacagg agagttcgct gctgaaagtg gtcccaaagg
     2461 ggtactagtt tttaagctcc caactccccc tcccccagcg tctggaggat tccacaccct
     2521 cgcaccgcag gggcgaggaa gtgggcggag tccggttttg gcgccagccg ctgaggctgc
     2581 caagcagaaa agccaccgct gaggagactc cggtcactgt cctcgccccg cctccccctt
     2641 ccctcccctt ggggaccacc gggcgccacg ccgcgaacgg taagtgccgc ggtcgtcggc
     2701 gcctccgccc tccccctagg gccccaattc ccagcgggcg cggcgcgcgg cccctccccc
     2761 cgccgggcgc gcgcccgctg ccccgccctt cgtggccgcc cggcgtgggc ggtgccaccc
     2821 ctccccccgg cggccccgcg cgcagctccc ggctccctcc cccttcggat gtggcttgag
     2881 ctgtaggcgc ggaggggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     2941 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
     3001 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     3061 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     3121 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     3181 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
     3241 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
     3301 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     3361 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     3421 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     3481 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     3541 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     3601 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     3661 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     3721 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     3781 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     3841 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     3901 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     3961 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     4021 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtggaattct
     4081 cacgcgtgac tctagcgttt aaacttaagc ttacggtacc atgaacatgg cgattatcaa
     4141 ggaattcatg aggttcaaag ttcacatgga gggatctgta aacggccacg agttcgaaat
     4201 cgagggagag ggggagggtc gaccatatga aggaacacag accgctaaat tgaaggttac
     4261 taaaggtgga ccacttcctt tcgcatggga cattcttagc ccccagttta tgtatggttc
     4321 caaggcgtat gtcaaacatc ccgcagacat tcctgattac cttaaactga gttttccaga
     4381 ggggttcaaa tgggagcgcg tcatgaactt tgaagatggg ggcgttgtta cagtgactca
     4441 ggactcttct ttgcaagatg gagagtttat atataaggtg aaacttcgcg gaaccaactt
     4501 cccaagcgat ggcccagtca tgcagaagaa aactatgggt tgggaagcca gtagcgagcg
     4561 gatgtaccca gaggacggcg cacttaaggg tgagattaag cagcggctga aactcaaaga
     4621 cggaggccat tatgacgcgg aagttaaaac gacgtacaaa gctaaaaagc ctgtccaatt
     4681 gccaggcgcg tacaatgtca atattaaatt ggacattacc agtcacaacg aagattacac
     4741 aatagtggag caatacgaac gcgcagaggg acgccattca acagggggaa gcggagctac
     4801 taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac ctggatccaa
     4861 cggaagagtg gattatttgg tcactgagga agagatcaat cttaccagag ggccctcagg
     4921 gctgggcttc aacatcgtcg gtgggacaga tcagcagtat gtctccaacg acagtggcat
     4981 ctacgtcagc cgcatcaaag aaaatggggc tgcggccctg gatgggcggc tccaggaggg
     5041 tgataagatc ctttcggtaa atggccaaga cctaaagaac ctgctgcacc aggatgctgt
     5101 agacctcttt cgtaatgcag gctatgctgt gtctctgaga gtgcagcaca ggttacaggt
     5161 gcagaatgga cctataggac atcgaggtga aggggaccca agtggtattc ccatatttat
     5221 ggtgctggtg ccagtgtttg ccctcaccat ggtagcagcc tgggctttca tgagataccg
     5281 gcaacaactt ggatcctccc gtgaccacat ggtccttcat gagtatgtaa atgctgctgg
     5341 gattacataa ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgacctgcag
     5401 gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     5461 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     5521 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     5581 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     5641 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     5701 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     5761 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     5821 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     5881 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     5941 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6001 ctttgggccg cctccccgca tcgataccgt cgacctcgag ggaattaatt cgagctcggt
     6061 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6121 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     6181 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6241 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6301 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6361 cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata
     6421 cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt
     6481 gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc
     6541 ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc
     6601 agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc
     6661 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     6721 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     6781 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     6841 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     6901 ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     6961 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     7021 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     7081 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     7141 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     7201 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7261 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7321 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7381 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7441 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7501 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7561 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7621 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7681 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7741 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7801 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7861 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7921 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7981 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8041 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8101 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8161 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8221 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8281 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8341 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8401 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8461 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8521 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8581 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8641 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8701 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8761 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8821 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8881 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8941 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9001 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9061 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9121 tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt
     9181 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     9241 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9301 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     9361 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9421 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9481 ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact
     9541 ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt
     9601 ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta
     9661 agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga
     9721 catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa
     9781 aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg
     9841 ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag
     9901 cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg
     9961 gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc
    10021 accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg
    10081 caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc
    10141 ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg
    10201 gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag
    10261 cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt
    10321 taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg
    10381 gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct
    10441 cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga
    10501 tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga
    10561 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct
    10621 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
    10681 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta
    10741 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg
    10801 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
    10861 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
    10921 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
    10981 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta
    11041 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta
    11101 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag
    11161 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
    11221 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
    11281 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    11341 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    11401 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
    11461 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
    11521 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
//
