LOCUS       sequence-450271-       13199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a reporter for an alpha-helical OMM protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13199)
  AUTHORS   Guna A, Stevens TA, Inglis AJ, Replogle JM, Esantsi TK, Muthukumar 
            G, Shaffer KCL, Wang ML, Pogson AN, Jones JJ, Lomenick B, Chou TF, 
            Weissman JS, Voorhees RM
  TITLE     MTCH2 is a mitochondrial outer membrane protein insertase.
  JOURNAL   Science. 2022 Oct 21;378(6617):317-322. doi: 
            10.1126/science.add1856. Epub 2022 Oct 20.
  PUBMED    36264797
REFERENCE   2  (bases 1 to 13199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.add1856"; journalName: "Science"; date: 
            "2022-10-21- 21"; volume: "378"; issue: "6617"; pages: "317-322"
COMMENT     Sequence Label: pAG426
FEATURES             Location/Qualifiers
     source          1..13199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    678..803
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2047..2164
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2896..4073
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3127..4065
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4022..4042
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4796..4852
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6929..6976
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     misc_feature    7060..7648
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7113..7133)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7531..7542)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7651..7667)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7652..7668)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7758..7991
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8016..8034)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8017..8034)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8324..8343)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8557..8576)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8676..8698
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8736..8754)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8822..8926
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8927..8995,8996..9787)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9145..9164)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9958..10546
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10447..10466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10700..10717
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10787..10807)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10792..11121
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10972..11107
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12255..12320
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12450..12470
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12895..13029
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12932..12951)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12986..13005
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(13197..13199,1..631)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
       61 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
      121 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
      181 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
      241 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
      301 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
      361 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
      421 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
      481 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      541 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      601 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
      661 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      721 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
      781 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
      841 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
      901 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
      961 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1021 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1081 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1141 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgccaaatgg cagtattcat
     2041 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2101 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     2161 ttttcgggtt tattacaggg acagcagaga tccagtttgg atcgataagc ttgatatcga
     2221 attcgggagg tggtccctgc agttacgcca atgataaccc ccgccagaaa aatcttagta
     2281 gccttccctt tttgttttcc gtgccccaac tcggcggatt gactcggccc cttccggaaa
     2341 cacccgaatc aacttctagt caaattattg ttcacgccgc aatgacccac ccctggcccg
     2401 cgtctgtgga actgacccct ggtgtacagg agagttcgct gctgaaagtg gtcccaaagg
     2461 ggtactagtt tttaagctcc caactccccc tcccccagcg tctggaggat tccacaccct
     2521 cgcaccgcag gggcgaggaa gtgggcggag tccggttttg gcgccagccg ctgaggctgc
     2581 caagcagaaa agccaccgct gaggagactc cggtcactgt cctcgccccg cctccccctt
     2641 ccctcccctt ggggaccacc gggcgccacg ccgcgaacgg taagtgccgc ggtcgtcggc
     2701 gcctccgccc tccccctagg gccccaattc ccagcgggcg cggcgcgcgg cccctccccc
     2761 cgccgggcgc gcgcccgctg ccccgccctt cgtggccgcc cggcgtgggc ggtgccaccc
     2821 ctccccccgg cggccccgcg cgcagctccc ggctccctcc cccttcggat gtggcttgag
     2881 ctgtaggcgc ggaggggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     2941 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
     3001 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     3061 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     3121 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     3181 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
     3241 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
     3301 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     3361 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     3421 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     3481 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     3541 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     3601 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     3661 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     3721 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     3781 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     3841 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     3901 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     3961 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     4021 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtggaattct
     4081 cacgcgtgac tctagcgttt aaacttaagc ttacggtacc atgaacatgg cgattatcaa
     4141 ggaattcatg aggttcaaag ttcacatgga gggatctgta aacggccacg agttcgaaat
     4201 cgagggagag ggggagggtc gaccatatga aggaacacag accgctaaat tgaaggttac
     4261 taaaggtgga ccacttcctt tcgcatggga cattcttagc ccccagttta tgtatggttc
     4321 caaggcgtat gtcaaacatc ccgcagacat tcctgattac cttaaactga gttttccaga
     4381 ggggttcaaa tgggagcgcg tcatgaactt tgaagatggg ggcgttgtta cagtgactca
     4441 ggactcttct ttgcaagatg gagagtttat atataaggtg aaacttcgcg gaaccaactt
     4501 cccaagcgat ggcccagtca tgcagaagaa aactatgggt tgggaagcca gtagcgagcg
     4561 gatgtaccca gaggacggcg cacttaaggg tgagattaag cagcggctga aactcaaaga
     4621 cggaggccat tatgacgcgg aagttaaaac gacgtacaaa gctaaaaagc ctgtccaatt
     4681 gccaggcgcg tacaatgtca atattaaatt ggacattacc agtcacaacg aagattacac
     4741 aatagtggag caatacgaac gcgcagaggg acgccattca acagggggaa gcggagctac
     4801 taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac ctggatccaa
     4861 gaaagacgtg cggatcctgc tggtgggaga acctagagtt gggaagacat cactgattat
     4921 gtctctggtc agtgaagaat ttccagaaga ggttcctccc cgggcagaag aaatcaccat
     4981 tccagctgat gtcaccccag agagagttcc aacacacatt gtagattact cagaagcaga
     5041 acagagtgat gaacaacttc atcaagaaat atctcaggct aatgtcatct gtatagtgta
     5101 tgccgttaac aacaagcatt ctattgataa ggtaacaagt cgatggattc ctctcataaa
     5161 tgaaagaaca gacaaagaca gcaggctgcc tttaatattg gttgggaaca aatctgatct
     5221 ggtggaatat agtagtatgg agaccatcct tcctattatg aaccagtata cagaaataga
     5281 aacctgtgtg gagtgttcag cgaaaaacct gaagaacata tcagagctct tttattacgc
     5341 acagaaagct gttcttcatc ctacagggcc cctgtactgc ccagaggaga aggagatgaa
     5401 accagcttgt ataaaagccc ttactcgtat atttaaaata tctgatcaag ataatgatgg
     5461 tactctcaat gatgctgaac tcaacttctt tcagaggatt tgtttcaaca ctccattagc
     5521 tcctcaagct ctggaggatg tcaagaatgt agtcagaaaa catataagtg atggtgtggc
     5581 tgacagtggg ttgaccctga aaggttttct ctttttacac acacttttta tccagagagg
     5641 gagacacgaa actacttgga ctgtgcttcg acgatttggt tatgatgatg acctggattt
     5701 gacacctgaa tatttgttcc ccctgctgaa aatacctcct gattgcacta ctgaattaaa
     5761 tcatcatgca tatttatttc tccaaagcac ctttgacaag catgatttgg atagagactg
     5821 tgctttgtca cctgatgagc ttaaagattt atttaaagtt ttcccttaca taccttgggg
     5881 gccagatgtg aataacacag tttgtaccaa tgaaagaggc tggataacct accagggatt
     5941 cctttcccag tggacgctca cgacttattt agatgtacag cggtgcctgg aatatttggg
     6001 ctatctaggc tattcaatat tgactgagca agagtctcaa gcttcagctg ttacagtgac
     6061 aagagataaa aagatagacc tgcagaaaaa acaaactcaa agaaatgtgt tcagatgtaa
     6121 tgtaattgga gtgaaaaact gtgggaaaag tggagttctt caggctcttc ttggaagaaa
     6181 cttaatgagg cagaagaaaa ttcgtgaaga tcataaatcc tactatgcga ttaacactgt
     6241 ttatgtatat ggacaagaga aatacttgtt gttgcatgat atctcagaat cggaatttct
     6301 aactgaagct gaaatcattt gtgatgttgt atgcctggta tatgatgtca gcaatcccaa
     6361 atcctttgaa tactgtgcca ggatttttaa gcaacacttt atggacagca gaataccttg
     6421 cttaatcgta gctgcaaagt cagacctgca tgaagttaaa caagaataca gtatttcacc
     6481 tactgatttc tgcaggaaac acaaaatgcc tccaccacaa gccttcactt gcaatactgc
     6541 tgatgccccc agtaaggata tctttgttaa attgacaaca atggccatgt atccagagga
     6601 tcattacaga gacagactct cccgagacat gggccacact gatagaatag agaatttgag
     6661 aaaaatctgg gtctttctaa aaactgcttt ccatgcccgg ttacgctgta tgtgcacctg
     6721 caacaggtgt acattttgca tctgtcagaa cttcctcaac tcagacttgc tgcaatctgt
     6781 aaagaacaaa atcttcactg cagttcttaa caggcacgtg acacaagctg acctcaagag
     6841 ctccacgttt tggcttcgag caagttttgg tgctactgtt tttgcagttt tgggctttgc
     6901 tatgtacaaa gcattattga aacagcgacg tgaccacatg gtccttcatg agtatgtaaa
     6961 tgctgctggg attacataac tcgagtctag agggcccgtt taaacccgct gatcagcctc
     7021 gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct ggattacaaa
     7081 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     7141 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7201 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7261 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7321 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7381 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7441 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     7501 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7561 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7621 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgagg gaattaattc
     7681 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7741 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     7801 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7861 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7921 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7981 aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg
     8041 tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag
     8101 cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc
     8161 gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg
     8221 gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc
     8281 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     8341 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     8401 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     8461 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     8521 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata
     8581 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     8641 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     8701 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     8761 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     8821 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     8881 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     8941 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     9001 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     9061 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     9121 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     9181 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     9241 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     9301 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     9361 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     9421 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     9481 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9541 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     9601 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     9661 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9721 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     9781 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     9841 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     9901 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     9961 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    10021 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    10081 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
    10141 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
    10201 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    10261 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    10321 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
    10381 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    10441 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    10501 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    10561 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    10621 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
    10681 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
    10741 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag
    10801 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    10861 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    10921 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
    10981 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    11041 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
    11101 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag
    11161 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
    11221 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
    11281 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
    11341 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
    11401 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg
    11461 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
    11521 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
    11581 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
    11641 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
    11701 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg
    11761 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
    11821 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
    11881 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
    11941 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat
    12001 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg
    12061 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga
    12121 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga
    12181 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
    12241 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
    12301 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
    12361 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
    12421 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
    12481 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
    12541 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
    12601 tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
    12661 tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
    12721 gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
    12781 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
    12841 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
    12901 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    12961 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    13021 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
    13081 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
    13141 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgg
//
