LOCUS       sequence-450273-        8877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPRi guide RNA against ATP13A1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8877)
  AUTHORS   Guna A, Stevens TA, Inglis AJ, Replogle JM, Esantsi TK, Muthukumar 
            G, Shaffer KCL, Wang ML, Pogson AN, Jones JJ, Lomenick B, Chou TF, 
            Weissman JS, Voorhees RM
  TITLE     MTCH2 is a mitochondrial outer membrane protein insertase.
  JOURNAL   Science. 2022 Oct 21;378(6617):317-322. doi: 
            10.1126/science.add1856. Epub 2022 Oct 20.
  PUBMED    36264797
REFERENCE   2  (bases 1 to 8877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.add1856"; journalName: "Science"; date: 
            "2022-10-21- 21"; volume: "378"; issue: "6617"; pages: "317-322"
COMMENT     Sequence Label: pAG392
FEATURES             Location/Qualifiers
     source          1..8877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    293..526
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             711..755
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1053..1170
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(1423..1456)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     1460..1480
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        1725..1936
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          1937..2875
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2832..2852
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2896..3492
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2896..2915)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3392..3412
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3502..3555
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3568..4266
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     protein_bind    complement(4286..4319)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4375..4963
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4428..4448)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4846..4857)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4966..4982)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4967..4983)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5492..5672
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(5734..6322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5814..5833)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6493..7284,7285..7353))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7116..7135
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7354..7458)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7533..7552)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7724..8103
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8105..8303
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8258..8278
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8321..8501
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8548..8673
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
       61 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
      121 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      181 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
      241 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
      301 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
      361 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
      421 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
      481 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
      541 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
      601 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
      661 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
      721 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
      781 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
      841 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
      901 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
      961 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     1021 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     1081 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     1141 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     1201 gtttggttag taccgggccc gctctagaga tccgacgcgc catctctagg cccgcgccgg
     1261 ccccctcgca cggacttgtg ggagaagctc ggctactccc ctgccccggt taatttgcat
     1321 ataatatttc ctagtaacta tagaggctta atgtgcgata aaagacagat aatctgttct
     1381 ttttaatact agctacattt tacatgatag gcttggattt ctataacttc gtatagcata
     1441 cattatacga agttataaac agcacaaaag gaaactcacc ctaactgtaa agtaattgtg
     1501 tgttttgaga ctataagtat cccttggaga accaccttgt tggggtaaag cagcccggcg
     1561 aagtttaaga gctaagctgg aaacagcata gcaagtttaa ataaggctag tccgttatca
     1621 acttgaaaaa gtggcaccga gtcggtgctt tttttctcga gtactaggat ccattaggcg
     1681 gccgcgtgga taaccgtatt accgccatgc atgtgcccgt cagtgggcag agcgcacatc
     1741 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
     1801 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     1861 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     1921 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
     1981 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
     2041 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
     2101 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
     2161 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
     2221 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
     2281 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
     2341 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
     2401 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
     2461 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
     2521 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
     2581 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     2641 ccgggcgccg tccaggcacc tcgattagtt ctcgcgcttt tggagtacgt cgtctttagg
     2701 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     2761 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     2821 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     2881 gtgagctagc ccaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     2941 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     3001 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     3061 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     3121 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     3181 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     3241 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     3301 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     3361 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     3421 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     3481 aagcccggtg ccggatcggg agagggcaga ggaagtctgc taacatgcgg tgacgtcgag
     3541 gagaatcctg gcccaccggt cgccaccagc gagctgatta aggagaacat gcacatgaag
     3601 ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc
     3661 aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc
     3721 ttcgccttcg acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac
     3781 acccagggca tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga
     3841 gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac
     3901 ggctgcctca tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg
     3961 atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc
     4021 ggcctggaag gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca
     4081 aacatcaaga ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc
     4141 tactatgtgg actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag
     4201 cagcacgagg tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt
     4261 aattaagaat tcgtcgaggg acctaataac ttcgtatagc atacattata cgaagttata
     4321 catgtttaag ggttccggtt ccactaggta caattcgata tcaagcttat cgataatcaa
     4381 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4441 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     4501 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4561 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4621 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4681 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4741 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     4801 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4861 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4921 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     4981 cgatcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     5041 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     5101 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     5161 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     5221 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     5281 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     5341 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     5401 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     5461 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     5521 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5581 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5641 acccttttag tcagtgtgga aaatctctag cagcatgtga gcaaaaggcc agcaaaaggc
     5701 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5761 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5821 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5881 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5941 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6001 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6061 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6121 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6181 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6241 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6301 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6361 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6421 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6481 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6541 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6601 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     6661 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     6721 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     6781 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     6841 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     6901 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     6961 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7021 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7081 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7141 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7201 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7261 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7321 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7381 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7441 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     7501 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     7561 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     7621 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     7681 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     7741 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     7801 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     7861 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     7921 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     7981 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     8041 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     8101 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     8161 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     8221 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     8281 cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca
     8341 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8401 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8461 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     8521 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     8581 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     8641 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     8701 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     8761 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     8821 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaa
//
