LOCUS       sequence-450281-       14006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing Vitis riparia FLS2 protein in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14006)
  TITLE     Coaker lab FLS2 plasmids
REFERENCE   2  (bases 1 to 14006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB514-35S:VrFLS2-HA
FEATURES             Location/Qualifiers
     source          1..14006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     488..507
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     741..758
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    773..913
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(839..858)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     999..1021
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1257..1451
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1517..2590)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3019..3648)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4949..4973
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     5161..5183
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5175..5192
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5176..5192
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5707..6052
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5995..6015
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    6074..6098
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9648..9672)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9701..9790
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      9824..10076
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(10109..10125)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10109..10125)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10122..10144)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10133..10149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10157..10163,10164..10181,10182..10187))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10202..10223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(10259..10511)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(10662..11687)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(11739..11918)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    12424..12448
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12969..13760
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(14006,1..587)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
       61 gcggtggttt gtttgccgga tcaaagctac caactctttt tccgaaggta actggcttca
      121 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
      181 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
      241 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
      301 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
      361 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      421 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      481 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      541 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      601 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      661 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      721 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
      781 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
      841 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
      901 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
      961 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     1021 gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt
     1081 cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca
     1141 tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca
     1201 gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg
     1261 cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat
     1321 cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct
     1381 ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg
     1441 tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca atttgcccta
     1501 gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc
     1561 gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt
     1621 catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc
     1681 cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc
     1741 gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa
     1801 ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct
     1861 cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca
     1921 aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca
     1981 tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc
     2041 cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc
     2101 cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg
     2161 ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct
     2221 ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg
     2281 acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga
     2341 gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg
     2401 caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg
     2461 attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca
     2521 acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc
     2581 gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcagac
     2641 aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc
     2701 gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct
     2761 actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct
     2821 cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg
     2881 gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag
     2941 ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt
     3001 tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac
     3061 ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt
     3121 gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc
     3181 cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa
     3241 ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc
     3301 ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc
     3361 cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc
     3421 gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt
     3481 tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc
     3541 gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt
     3601 cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg
     3661 cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt
     3721 tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg
     3781 gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg
     3841 gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc
     3901 tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct
     3961 cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga
     4021 aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt
     4081 acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct
     4141 ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa
     4201 acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg
     4261 aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg
     4321 tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct
     4381 cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca
     4441 caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg
     4501 tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt
     4561 ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg
     4621 cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac
     4681 taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt
     4741 aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg
     4801 cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa ccctgtggtt
     4861 ggcatgcaca tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat
     4921 tattctaata aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg
     4981 atagtttaaa ctgaaggcgg gaaacgacaa tctgatccaa gctcaagctg ctctagcatt
     5041 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     5101 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     5161 cccagtcacg acgttgtaaa acgacggcca gtgccaagct tgcatgcctg caggtcccca
     5221 gattagcctt ttcaatttca gaaagaatgc taacccacag atggttagag aggcttacgc
     5281 agcaggtctc atcaagacga tctacccgag caataatctc caggaaatca aataccttcc
     5341 caagaaggtt aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa
     5401 agatatattt ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca
     5461 caaaccaagg caagtaatag agattggagt ctctaaaaag gtagttccca ctgaatcaaa
     5521 ggccatggag tcaaagattc aaatagagga cctaacagaa ctcgccgtaa agactggcga
     5581 acagttcata cagagtctct tacgactcaa tgacaagaag aaaatcttcg tcaacatggt
     5641 ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag
     5701 ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc
     5761 agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca
     5821 tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga
     5881 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     5941 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
     6001 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagaa cacgggggac
     6061 tctagagtta tcaacaagtt tgtacaaaaa agcaggctcc gcggccgccc ccttcaccat
     6121 ggtgtctgaa agagtcagtt taatcctatt cctggtttgt tcatttctag tactggtacc
     6181 accagttcta accatggagc caagcttaga agttgaacat gaggccttga aggcctttaa
     6241 gaattcagtt gctgacgacc cctttggagc ccttgcagat tggagtgaag ccaaccatca
     6301 ctgcaattgg tctggaatca catgtgacct ttcatccaat catgtcatat cggtctcgct
     6361 gatggagaag cagcttgcag gtcagatctc tccattcctt ggaaacattt ccatcctcca
     6421 ggttctagac ttgagttcca actcattcac tggccacatt ccacctcagc tggggctttg
     6481 ctcccagctt ttagaattga atcttttcca aaattctctt tctggatcaa ttccaccaga
     6541 actaggaaac cttagaaatc tgcagtcatt agacttgggg agtaacttct tggaaggaag
     6601 catccccaaa agcatttgca actgcactgc cttgttggga cttggcatca tcttcaacaa
     6661 tctcacaggt accatcccaa cagacattgg aaatttagct aatcttcaaa tccttgtact
     6721 atatagcaac aatattattg gtcctatacc agtttccata ggtaagttgg gagatttgca
     6781 atctctagac ctaagtatca accaattgtc tggtgtaatg cctccagaga tcgggaactt
     6841 atcgaactta gaatatcttc aattgtttga gaatcgcctt tctggaaaaa tcccatcaga
     6901 actggggcag tgtaagaagc tgatctatct gaacttatac agcaaccaat ttacaggagg
     6961 cattccttct gagctaggaa acttggtcca gttggttgct ctgaagttgt acaaaaatag
     7021 gttaaattca acaattccaa gctccttatt ccagctgaaa tatttgactc acttaggaat
     7081 ctctgagaat gagttgattg gaactattcc ctctgagctt ggatctttga gatcactgca
     7141 ggtactaact ttgcattcaa ataagttcac tgggaaaatc cctgcacaga taaccaactt
     7201 gacaaacttg acaattttgt caatgagctt caacttcctc acaggggagc ttccatccaa
     7261 tatcggatcc cttcataatt tgaagaacct gaccatgcac aacaaccttc tagagggatc
     7321 cattccatct agtatcacca attgcaccca tcttgtcaac atcggtttag cttataatat
     7381 gataacaggg gaaattccac aaggtttggg acagttgccg aatcttactt tccttggttt
     7441 aggggttaac aaaatgtcgg ggaacattcc tgacgacctc ttcaattgct caaatctggc
     7501 tatcctagat ttggccagga acaatttcag tggagttctg aaaccaggca ttggcaagct
     7561 ctataatctg caaagattgc aagcccacaa gaattcctta gtagggccta tcccacctga
     7621 gattggcaat ttaagtcaat tgttctcctt acagctgaat gggaatagtt tgtcaggcac
     7681 agttcctcct gaattatcca agctttctcc ccttcagggt ctgtacctcg atgacaatgc
     7741 cctagaaggt gcaattcctg aggaaatttt tgaactaaaa catctcagtg agcttggttt
     7801 gggtgacaac cggttcgcag gacacattcc acatgctgtc tctaaacttg agtcgctttt
     7861 gaacttgtac ctcaatggaa acgtgctcaa tggatccatc ccagcaagca tggctcgtct
     7921 ttctcggctg gcaatcttgg atctctctca caaccatctt gtcgggtcaa taccgggacc
     7981 tgtcattgct agtatgaaaa acatgcagat atatctcaac ttttcacaca atttcttatc
     8041 aggacccatt ccagatgaga ttgggaaatt ggagatggtc cagatcgtag atatgtcaaa
     8101 caataatctg tcagggagca ttccagagac tcttcaaggc tgcagaaact tgttcaatct
     8161 tgatctgtca gtgaatgagc tttctggtcc agttccagag aaggcatttg cccaaatgga
     8221 tgtacttaca agcttgaacc tttcaagaaa taatttaaat ggtggactac ctggaagctt
     8281 agcgaatatg aagaatctaa gctctctgga cctttctcag aacaagttca agggcatgat
     8341 cccggagagt tatgcaaata tttccacctt gaagcagctg aatctttctt tcaatcaact
     8401 ggaaggccgt gttccagaga ctgggatatt caaaaacgta agtgcatctt ctttggtggg
     8461 aaacccaggt ctttgtggaa ccaaatttct agggtcatgc aggaataaga gccatttagc
     8521 tgcttctcat cgattctcca agaaaggtct gttgattctc ggggtacttg gatccctcat
     8581 tgtattgctg cttctcacat tttctgttat aatcttctgt cggtacttca gaaagcaaaa
     8641 aacagttgaa aatccagaac cagagtatgc ttcagcattg acgctcaaga ggttcaatca
     8701 aaaggattta gaaatagcta ccggcttctt tagtgcagaa aacatcattg gggccagcac
     8761 tttgagtacg gtatacaagg gcagaacgga agatgggaag attgtagccg taaagaaatt
     8821 gaatttgcaa cagttctctg ctgaggctga taagtgcttc aatagagaag tgaagaccct
     8881 gagccgactc aggcacagaa acctcgtaaa ggtacttggg tatgcttggg aaagtggaaa
     8941 aataaaggct ctagttctgg aatacatgga gaaggggaac ttggacagca ttatacatga
     9001 accaggggtg gatcattcaa ggtggacttt attggagaga atcaatgttt gtatttccat
     9061 tgcaaggggg ctggtttact tgcattcagg gtatgatttc cccattgtcc actgtgactt
     9121 gaagccttct aatgttcttt tggatggaga tttggaggcc catgtgagtg attttggaac
     9181 atctcgagtt ctgggtgttc atctccagga tggaagcagt gtttccttat catcagcatt
     9241 tgaaggcaca atcggatact tggcaccaga gttcgcatac atgagggagc tcacaaccaa
     9301 agtcgatgtg ttcagctttg ggatcatagt aatggagttc ctaacaaaaa gaaggccaac
     9361 aggactggca gcagaggatg gcttgccatt gaccttgcgt cagctggtgg atgcggccct
     9421 tgccagtgga tccaaaagac ttcttcaaat agtggaccct ttcttagctt caattgtaac
     9481 agcgaacgag ggggaagtac tggaaaagct gcttaaactg gccttgtctt gcacctgcac
     9541 tgagcctggg gatcgacccg acatgaatga ggtgttgtcc agtcttttga agctaggagc
     9601 aaacatacca cctccattac catcatcatc aaagggtggg cgcgccgacc cagctttctt
     9661 gtacaaagtg gttgataaca gcgggttaat taacatcttt tacccatacg atgttcctga
     9721 ctatgcgggc tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc
     9781 agattacgct gctcagtgca gcgcttagag ctcgaatttc cccgatcgtt caaacatttg
     9841 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     9901 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     9961 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
    10021 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
    10081 attggttccg gaaccaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc
    10141 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    10201 aatgagtgag ctaactcaca ttaggctgaa ttaggcgcgc ctatttctat taattcccga
    10261 tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg
    10321 ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata
    10381 aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat
    10441 atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg
    10501 tttgaacgat cggggaaatt cgggggatcg atcccgcgaa ctgtggacga gaactgtgcc
    10561 accaagcgta aggccgttct ctcgcatttg ccttgctagg ctcgcgcgag ttgctggctg
    10621 aggcgttctc gaaatcagct cttgttcggt cggcatctac tctattcctt tgccctcgga
    10681 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag
    10741 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg
    10801 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc
    10861 tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg cgatcctgca
    10921 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc
    10981 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag
    11041 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg
    11101 tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc
    11161 tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga
    11221 tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg
    11281 aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catggcctcc
    11341 gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc
    11401 tgtgcacggc gggagatgca ataggtcagg ctctcgctga atgccccaat gtcaagcact
    11461 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa
    11521 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa
    11581 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg
    11641 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc ttattgcccc
    11701 ccgggatcag atctggattg agagtgaata tgagactcta attggatacc gaggggaatt
    11761 tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata gtgaccttag
    11821 gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat taaactccag
    11881 aaacccgcgg ctgagtggct ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg
    11941 gcttgtcccg cgtcatcggc gggggtcata acgtgactcc cttaattctc cgctcatgat
    12001 cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
    12061 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat
    12121 aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt
    12181 gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac
    12241 cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgag
    12301 tgcttgcggc agcgtgaagc tgatccgtcg agattaatag aaataaattc agcctaattc
    12361 ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa
    12421 tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct
    12481 cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag
    12541 agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac
    12601 taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga
    12661 tgacagagcg ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctgtt
    12721 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
    12781 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
    12841 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
    12901 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
    12961 taaacatcat gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
    13021 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
    13081 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
    13141 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
    13201 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
    13261 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
    13321 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
    13381 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
    13441 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
    13501 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
    13561 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
    13621 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
    13681 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
    13741 ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg
    13801 gcgcggctta actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc
    13861 aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag
    13921 atatatcatg catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    13981 ccgtagaaaa gatcaaagga tcttct
//
