LOCUS       sequence-450285-       14531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing Quercus variabilis FLS2 protein in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14531)
  TITLE     Coaker lab FLS2 plasmids
REFERENCE   2  (bases 1 to 14531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB514-35S:QvFLS2-HA
FEATURES             Location/Qualifiers
     source          1..14531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     489..508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     742..759
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    774..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1258..1452
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1518..2591)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3020..3649)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4950..4974
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     5162..5184
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5176..5193
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5177..5193
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5708..6053
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5996..6016
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    6075..6099
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10174..10198)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10227..10316
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      10350..10602
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(10635..10651)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10635..10651)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10648..10670)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10659..10675
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10683..10689,10690..10707,10708..10713))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10728..10749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(10785..11037)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11188..12213)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(12265..12444)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    12950..12974
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             13495..14286
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaaagcta ccaactcttt ttccgaaggt aactggcttc
      121 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
      181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
      781 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg
     1081 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc
     1141 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc
     1201 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat
     1261 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa
     1321 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc
     1381 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac
     1441 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct
     1501 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag
     1561 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg
     1621 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg
     1681 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg
     1741 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga
     1801 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc
     1861 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc
     1921 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc
     1981 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt
     2041 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg
     2101 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc
     2161 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc
     2221 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc
     2281 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag
     2341 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc
     2401 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac
     2461 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc
     2521 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg
     2581 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga
     2641 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg
     2701 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc
     2761 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc
     2821 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc
     2881 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca
     2941 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt
     3001 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga
     3061 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag
     3121 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg
     3181 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa
     3241 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt
     3301 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg
     3361 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt
     3421 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg
     3481 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat
     3541 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg
     3601 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt
     3661 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt
     3721 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct
     3781 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac
     3841 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat
     3901 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc
     3961 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg
     4021 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg
     4081 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc
     4141 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca
     4201 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg
     4261 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg
     4321 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc
     4381 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc
     4441 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg
     4501 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt
     4561 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg
     4621 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga
     4681 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact
     4741 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact
     4801 gcactatagc agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt
     4861 tggcatgcac atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga
     4921 ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact
     4981 gatagtttaa actgaaggcg ggaaacgaca atctgatcca agctcaagct gctctagcat
     5041 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     5101 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     5161 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc ttgcatgcct gcaggtcccc
     5221 agattagcct tttcaatttc agaaagaatg ctaacccaca gatggttaga gaggcttacg
     5281 cagcaggtct catcaagacg atctacccga gcaataatct ccaggaaatc aaataccttc
     5341 ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga
     5401 aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc
     5461 acaaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttccc actgaatcaa
     5521 aggccatgga gtcaaagatt caaatagagg acctaacaga actcgccgta aagactggcg
     5581 aacagttcat acagagtctc ttacgactca atgacaagaa gaaaatcttc gtcaacatgg
     5641 tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa
     5701 gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc
     5761 cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc
     5821 atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag
     5881 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
     5941 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
     6001 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagaga acacggggga
     6061 ctctagagtt atcaacaagt ttgtacaaaa aagcaggctc cgcggccgcc cccttcacca
     6121 tggtatctca aaaaattggt ttgattgtaa tcatagtttg ttcccttcta gttgctgttc
     6181 tatctgaaga atcaagctta gaagttcaac ttgaggcctt gaatgctttc aagaattcca
     6241 tcaccaatga cccatttgga gcacttgcgg attggactga cagtaaccac cactgcaact
     6301 ggtccggtat tgcttgtgac ccttcaaatc atgtcatttc aatttctctt attgataagc
     6361 agcttaaagg tgaaatcaca ccattccttg gaaacctttc aagcctccag gttcttgatt
     6421 taacgttgaa ttcatttaca ggccacattc cagctcaact gggactttgc tcccagctct
     6481 ctgagctaac tctttatgaa aattctcttt caggtccaat tccaccagaa ctaggaaacc
     6541 taaaaaatct gcagtccgta gatttaggtg ataactcttt gaatggaagc atccccgaaa
     6601 gcatttgtaa ctgcacatca ttgttagcct ttggtgtcat ttttaataat ctaactggaa
     6661 aaatcccttc aaacataggc aatttagcta accttcaact ctttttagta tatgggaaca
     6721 agttggttgg ttctatacct gtatccattg gtaggttgga atctttacaa gctttagacc
     6781 tgagtcaaaa ccaattctct ggagtaattc ctccacagat aggaaactta tcaaatttag
     6841 agtatcttct cttgtttgaa aattcctttc ttgggaaaat tccatctgaa ctaggtcgct
     6901 gtaaacagct tgttgctctt gaactataca ccaatcagtt cactggaggc attccacttg
     6961 agattggata tttgtatcat ctagaaacat tgcggttgta caagaataag ttaaattcta
     7021 ccattcctgc ctccatattt caattgaaat cattaaccca tttaggactt tcggagaatg
     7081 aattaactgg aataataccc tttgagctag gatcattgag atcattggaa gtgttgaccc
     7141 tgcattcaaa taagttctct ggggaggttc cttcgtcatt gacgaacttg acaaatttaa
     7201 cccagttgtc tatgagcttc aatttcctca cagggaagct tccatcagat attggattgc
     7261 tttacaactt gaagaaccta accatgaaca acaatctcct tgagggagcc attccgtcta
     7321 gtatcaccaa ctgcacccat cttctagtca taggtttatc tcgtaacaga ataacaggga
     7381 aaattcctca gggttttcag cagttgcaaa atcttacttt cctgagtctg tcgtacaata
     7441 aaatggtagg tgtaatacca gatggtctct tcaattgctc caatcttcgt attttagatt
     7501 tgactcagaa cttttttaga gggccaataa agtctggcat tggcaaactt tctaatctcc
     7561 agattttgcg agcccattct aattcattca tagggccaat cccacctgac actggcaatt
     7621 taagtaaact tttttcttta tcacttggtc aaaatagttt gtcaggtcta gttccaccag
     7681 aattatctaa actttctctt ctccaagggc tctatctgaa tgacaatgat ctagagggtg
     7741 caatacctga gaattttttt gaaatgaaac agctaaacga acttgggttg cagaataata
     7801 agttcaaggg tccaattcca gatgcaattt ctaagcttga gcagctttct tacttaaacc
     7861 tccatggcaa catgcttaac gggtccattc tgagaagcat ggggcatctt aaccaactta
     7921 tgaccttgga tctctcccac aattatctta caggatcaat acctgcatca gtgcttgcaa
     7981 gtatgaaaag aatgcagtta tatttgaact tctcatacaa tttcttggtt ggaactattc
     8041 cagatgagct tggaatgttg gaaatggttc aagccattga catctcaaac aataatcttt
     8101 cggggaccat tcctaaaaca attggtggct gcaaaaattt gttctctctt gatttatcag
     8161 ggaataatct ttctggtcca attccggctg aagaatttac tcaaatggtt atgcttagta
     8221 gcttgaacct ttcaaggaac aagttagatg gtggactccc tgaagacctg gcagctctga
     8281 agtaccttag ctctcttgac ctttctcaga atcagttgaa gggcattatc cctgagagct
     8341 ttgccaatct ctctaacttg aaatatctaa acctttcttt caatcaactt gaaggccatg
     8401 ttccagagat tggaatattc agacatatta actcatctag tttgatggga aaccctcatc
     8461 tgtgtggtat tgaattcctc aggtcctgca gcaaattgag ttcacatcaa ctatccaaga
     8521 agaccatgtt gattcttgtg gtgattggat ctatttttgt gcttttagtt cttgtgttca
     8581 tatttatatt cctccaccaa cgtacaaagt taagaaaacc agaaggggtt gagaatccag
     8641 aatcagaata tactccaccc ttgaccctca agaggtttgt accatatgaa ttagaacatg
     8701 ctactagctt ctttagtgaa gataacatta ttggtgcaag cagtttgagc actgtgtaca
     8761 agggtagact ggaagatggg cagatcatag cagtaaaaaa gttgaatttg catcagttct
     8821 ctgaagaggc caataagtgc tttgataggg agatcaacac cctaaaacaa ctgaggcata
     8881 ggaatttggt gaaggttcta gggtatgcct gggagagtgg aaaactaaag gctttagttc
     8941 tggaatacat ggagaacggg aatttggata gtattataca tgaccctgag gtggatcagt
     9001 caaggtggac attatcagag cggatcaaag ttttcatttc cattgcgact gggttggatt
     9061 acctgcattc aggttatgat tttcctatcg ttcactgtga catgaagcct tctaacattc
     9121 ttctggatga agattgggaa gcccatgtaa gtgactttgg aacagctcga atgctaggtg
     9181 ttcatcttca agatggaaga agtctgtctg caacatcagc atttgaaggc actataggct
     9241 acttggcacc aggtaactta tcactatcct ttctcactta tgaagttaat ctccattaat
     9301 ttcgctgtca gatttacaag atcatttcat tgtcataaat tttcagtaga aaatgagtga
     9361 aatagtttta acaatcacaa tcagtttgga ccagggattc agtcttgcac atataaatac
     9421 caggtttaac gtagcaaact aaaatcctaa agcattgcat atatatatat atatatatag
     9481 tgctctattc agctatttgt aaacaatgct aaggtctcca tggctatttc actttcagaa
     9541 gtcataaata attattaaaa aattaagttc ttttaatgaa aagcgactgg agcattataa
     9601 gcttggaaga taaatctgtg tcatccttta attaaaacat gaatgcagtg gttggtttta
     9661 cataacaaag gtcccagagg tatatatgaa gccactgaat tcaaatacca gatatcaagt
     9721 agggatataa gttattttgc ttttcccatc ttcagatttc ccctttttat tccacttgtt
     9781 tgtcttcaat atactaaaac atgtttgttt tcttatcaga gtttgcatat atgacgaaag
     9841 tcacaactaa agttgacata ttcagctttg gtatcatagt catggaattt ctcactaaac
     9901 gaaggccaac aggactaata gaagaggatg ggctgccaat cagtttgcgt caacttgtgg
     9961 agaaagctct tgcaaatgga gagaacgatc ttcatcgaat tgtggatcct atgctggcac
    10021 tgaatatctc cgaggatcat gaggaattac tgaaagagct acttaagata gcattgcttt
    10081 gcacacaacc aaatcctgag gatcgacctg acataaatga ggtgctgtct atccttttga
    10141 agctaagcaa gcgcataaag ggtgggcgcg ccgacccagc tttcttgtac aaagtggttg
    10201 ataacagcgg gttaattaac atcttttacc catacgatgt tcctgactat gcgggctatc
    10261 cctatgacgt cccggactat gcaggatcct atccatatga cgttccagat tacgctgctc
    10321 agtgcagcgc ttagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct
    10381 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
    10441 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
    10501 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
    10561 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg gttccggaac
    10621 caattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    10681 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    10741 ctcacattag gctgaattag gcgcgcctat ttctattaat tcccgatcta gtaacataga
    10801 tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta
    10861 ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca
    10921 ttacatgtta attattacat gcttaacgta attcaacaga aattatatga taatcatcgc
    10981 aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg aacgatcggg
    11041 gaaattcggg ggatcgatcc cgcgaactgt ggacgagaac tgtgccacca agcgtaaggc
    11101 cgttctctcg catttgcctt gctaggctcg cgcgagttgc tggctgaggc gttctcgaaa
    11161 tcagctcttg ttcggtcggc atctactcta ttcctttgcc ctcggacgag tgctggggcg
    11221 tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct
    11281 gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg
    11341 accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc
    11401 aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct ccggatgcct
    11461 ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat
    11521 gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt
    11581 tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg
    11641 gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc
    11701 actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca
    11761 tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc
    11821 gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga ccggctgcag
    11881 aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga
    11941 gatgcaatag gtcaggctct cgctgaatgc cccaatgtca agcacttccg gaatcgggag
    12001 cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct
    12061 atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc
    12121 gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc
    12181 gacagacgtc gcggtgagtt caggcttttt catatcttat tgccccccgg gatcagatct
    12241 ggattgagag tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt
    12301 ggagcatttt tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg
    12361 cgcaataatg gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctga
    12421 gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc
    12481 atcggcgggg gtcataacgt gactccctta attctccgct catgatcttg atcccctgcg
    12541 ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt
    12601 accagagggc gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc
    12661 tagctatcgc catgtaagcc cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc
    12721 ccttgtccag atagcccagt agctgacatt catccggggt cagcaccgtt tctgcggact
    12781 ggctttctac gtgttccgct tcctttagca gcccttgcgc cctgagtgct tgcggcagcg
    12841 tgaagctgat ccgtcgagat taatagaaat aaattcagcc taattcggcg ttaattcagt
    12901 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac
    12961 aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac
    13021 cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc
    13081 ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt
    13141 tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc
    13201 tgcctgtgat caattcgggc acgaacccag tggacataag cctgttcggt tcgtaagctg
    13261 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
    13321 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
    13381 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
    13441 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgggg
    13501 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
    13561 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
    13621 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
    13681 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
    13741 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
    13801 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
    13861 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
    13921 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
    13981 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
    14041 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
    14101 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
    14161 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
    14221 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
    14281 aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc
    14341 aagcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct
    14401 tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgcatg
    14461 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    14521 aaaggatctt c
//
