LOCUS       sequence-450289-       14006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing SynVrFLS2_22 protein in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14006)
  TITLE     Coaker lab FLS2 plasmids
REFERENCE   2  (bases 1 to 14006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB514-35S:SynVrFLS2_22-HA
FEATURES             Location/Qualifiers
     source          1..14006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             127..918
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1164..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1937..2077
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2003..2022)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2163..2185
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2421..2615
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2681..3754)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4183..4812)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6113..6137
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6325..6347
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6339..6356
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6340..6356
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6871..7216
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     7159..7179
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    7238..7262
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10812..10836)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10865..10954
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      10988..11240
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(11273..11289)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11273..11289)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11286..11308)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11297..11313
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11321..11327,11328..11345,11346..11351))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11366..11387
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(11423..11675)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11826..12851)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(12903..13082)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    13588..13612
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
       61 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
      121 aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
      181 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
      241 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
      301 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
      361 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
      421 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
      481 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
      541 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
      601 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
      661 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
      721 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
      781 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
      841 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
      901 aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc
      961 gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag
     1021 gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat
     1081 atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1141 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1201 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     1981 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     2041 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     2101 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     2161 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     2221 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
     2281 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
     2341 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
     2401 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
     2461 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
     2521 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
     2581 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
     2641 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
     2701 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
     2761 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
     2821 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
     2881 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
     2941 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
     3001 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
     3061 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
     3121 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
     3181 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
     3241 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
     3301 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
     3361 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
     3421 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
     3481 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
     3541 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
     3601 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
     3661 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
     3721 tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc
     3781 agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc
     3841 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
     3901 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
     3961 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
     4021 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
     4081 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
     4141 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
     4201 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
     4261 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
     4321 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
     4381 tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg
     4441 ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac
     4501 cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg
     4561 cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt
     4621 cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg
     4681 ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc
     4741 gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc
     4801 gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca
     4861 gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc
     4921 tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg
     4981 gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg
     5041 gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc
     5101 tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg
     5161 cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt
     5221 cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg
     5281 cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg
     5341 gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc
     5401 tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca
     5461 acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc
     5521 ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca
     5581 ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg
     5641 ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc
     5701 cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg
     5761 catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt
     5821 cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca
     5881 gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct
     5941 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt
     6001 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt
     6061 ttcacgccct tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc
     6121 gccaatatat cctgtcaaac actgatagtt taaactgaag gcgggaaacg acaatctgat
     6181 ccaagctcaa gctgctctag cattcgccat tcaggctgcg caactgttgg gaagggcgat
     6241 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     6301 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
     6361 agcttgcatg cctgcaggtc cccagattag ccttttcaat ttcagaaaga atgctaaccc
     6421 acagatggtt agagaggctt acgcagcagg tctcatcaag acgatctacc cgagcaataa
     6481 tctccaggaa atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac
     6541 taactgcatc aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt
     6601 atggacgatt caaggcttgc ttcacaaacc aaggcaagta atagagattg gagtctctaa
     6661 aaaggtagtt cccactgaat caaaggccat ggagtcaaag attcaaatag aggacctaac
     6721 agaactcgcc gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa
     6781 gaagaaaatc ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa
     6841 agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg
     6901 aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa
     6961 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc
     7021 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     7081 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
     7141 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     7201 tcatttggag agaacacggg ggactctaga gttatcaaca agtttgtaca aaaaagcagg
     7261 ctccgcggcc gcccccttca ccatggtgtc tgaaagagtc agtttaatcc tattcctggt
     7321 ttgttcattt ctagtactgg taccaccagt tctaaccatg gagccaagct tagaagttga
     7381 acatgaggcc ttgaaggcct ttaagaattc agttgctgac gacccctttg gagcccttgc
     7441 agattggagt gaagccaacc atcactgcaa ttggtctgga atcacatgtg acctttcatc
     7501 caatcatgtc atatcggtct cgctgatgga gaagcagctt gcaggtcaga tctctccatt
     7561 ccttggaaac atttccatcc tccaggttct agacttgagt tccaactcat tcactggcca
     7621 cattccacct cagctggggc tttgctccca gcttttagaa ttgaatcttt tccaaaattc
     7681 tctttctgga tcaattccac cagaactagg aaaccttaga aatctgcagt cattagactt
     7741 ggggagtaac ttcttggaag gaagcatccc caaaagcatt tgcaactgca ctgccttgtt
     7801 gggacttggc atcatcttca acaatctcac aggtaccatc ccaacagaca ttggaaattt
     7861 agctaatctt caaatccttg tactatatag caacaatatt attggtccta taccagtttc
     7921 cataggtaag ttgggagatt tgcaatctct agacctaagt atcaaccaat tgtctggtgt
     7981 aatgcctcca gagatcggga acttatcgaa cttagaatat cttcaattgt ttgagaatcg
     8041 cctttctgga aaaatcccat cagaactggg gcagtgtaag aagctgatct atctgaactt
     8101 atacagcaac caatttacag gaggcattcc ttctgagcta ggaaacttgg tccagttggt
     8161 tgctctgaag ttgtacaaaa ataggttaaa ttcaacaatt ccaagctcct tattccagct
     8221 gaaatatttg gtccatttag gaatatccga aaatgagctt atcggcacga taccctctga
     8281 gttaggcagt ttgcgtagtt tgcaagtgtt gacattgcaa ttgaacaagt ttaccgggaa
     8341 gattccagcc caaataacta atttgaccaa tttaacttac ttatcaatgg acttcaactt
     8401 ccttacgggc gaactgccta gtaacatagg aagtttgcac aacttgaaga acttgacatt
     8461 aaataacaac cttctcgaag gaagcattcc ttcatccata actaattgta cccgtttggt
     8521 cgtcatcggc ttggcctata atatgattac gggtgaaatc ccacagggac ttggacagct
     8581 cccgaacctc atatttctga gtttcggcaa gaaccaaatg tccggtaaca tccccgatga
     8641 cctgtttaac tgttctaacc ttgcaatcct ggatttggcc aaaaacaact tctccggtgt
     8701 actcaaacca gggattggga agctctataa cctgcatatt tttcaggcac acaaaaactc
     8761 actcgttggt ccgataccac ctgaaatagg taatctcagt caactctttt ccctgaaact
     8821 taatggaaat agcctgtccg ggacggtacc accagaactt agcaagctct cattacttca
     8881 ggggctttac ttggacgata acgctttgga aggggccata cccgaggaaa tatttgagct
     8941 taagcaatta agtgaccttg gtttgggtga caaccggttc gcaggacaca ttccacatgc
     9001 tgtctctaaa cttgagtcgc ttttgaactt gtacctcaat ggaaacgtgc tcaatggatc
     9061 catcccagca agcatggctc gtctttctcg gctggcaatc ttggatctct ctcacaacca
     9121 tcttgtcggg tcaataccgg gacctgtcat tgctagtatg aaaaacatgc agatatatct
     9181 caacttttca cacaatttct tatcaggacc cattccagat gagattggga aattggagat
     9241 ggtccagatc gtagatatgt caaacaataa tctgtcaggg agcattccag agactcttca
     9301 aggctgcaga aacttgttca atcttgatct gtcagtgaat gagctttctg gtccagttcc
     9361 agagaaggca tttgcccaaa tggatgtact tacaagcttg aacctttcaa gaaataattt
     9421 aaatggtgga ctacctggaa gcttagcgaa tatgaagaat ctaagctctc tggacctttc
     9481 tcagaacaag ttcaagggca tgatcccgga gagttatgca aatatttcca ccttgaagca
     9541 gctgaatctt tctttcaatc aactggaagg ccgtgttcca gagactggga tattcaaaaa
     9601 cgtaagtgca tcttctttgg tgggaaaccc aggtctttgt ggaaccaaat ttctagggtc
     9661 atgcaggaat aagagccatt tagctgcttc tcatcgattc tccaagaaag gtctgttgat
     9721 tctcggggta cttggatccc tcattgtatt gctgcttctc acattttctg ttataatctt
     9781 ctgtcggtac ttcagaaagc aaaaaacagt tgaaaatcca gaaccagagt atgcttcagc
     9841 attgacgctc aagaggttca atcaaaagga tttagaaata gctaccggct tctttagtgc
     9901 agaaaacatc attggggcca gcactttgag tacggtatac aagggcagaa cggaagatgg
     9961 gaagattgta gccgtaaaga aattgaattt gcaacagttc tctgctgagg ctgataagtg
    10021 cttcaataga gaagtgaaga ccctgagccg actcaggcac agaaacctcg taaaggtact
    10081 tgggtatgct tgggaaagtg gaaaaataaa ggctctagtt ctggaataca tggagaaggg
    10141 gaacttggac agcattatac atgaaccagg ggtggatcat tcaaggtgga ctttattgga
    10201 gagaatcaat gtttgtattt ccattgcaag ggggctggtt tacttgcatt cagggtatga
    10261 tttccccatt gtccactgtg acttgaagcc ttctaatgtt cttttggatg gagatttgga
    10321 ggcccatgtg agtgattttg gaacatctcg agttctgggt gttcatctcc aggatggaag
    10381 cagtgtttcc ttatcatcag catttgaagg cacaatcgga tacttggcac cagagttcgc
    10441 atacatgagg gagctcacaa ccaaagtcga tgtgttcagc tttgggatca tagtaatgga
    10501 gttcctaaca aaaagaaggc caacaggact ggcagcagag gatggcttgc cattgacctt
    10561 gcgtcagctg gtggatgcgg cccttgccag tggatccaaa agacttcttc aaatagtgga
    10621 ccctttctta gcttcaattg taacagcgaa cgagggggaa gtactggaaa agctgcttaa
    10681 actggccttg tcttgcacct gcactgagcc tggggatcga cccgacatga atgaggtgtt
    10741 gtccagtctt ttgaagctag gagcaaacat accacctcca ttaccatcat catcaaaggg
    10801 tgggcgcgcc gacccagctt tcttgtacaa agtggttgat aacagcgggt taattaacat
    10861 cttttaccca tacgatgttc ctgactatgc gggctatccc tatgacgtcc cggactatgc
    10921 aggatcctat ccatatgacg ttccagatta cgctgctcag tgcagcgctt agagctcgaa
    10981 tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
    11041 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
    11101 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
    11161 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
    11221 gtcatctatg ttactagatc gggaattggt tccggaacca attcgtaatc atgtcatagc
    11281 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    11341 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaggc tgaattaggc
    11401 gcgcctattt ctattaattc ccgatctagt aacatagatg acaccgcgcg cgataattta
    11461 tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact
    11521 ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc
    11581 ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat
    11641 cttaagaaac tttattgcca aatgtttgaa cgatcgggga aattcggggg atcgatcccg
    11701 cgaactgtgg acgagaactg tgccaccaag cgtaaggccg ttctctcgca tttgccttgc
    11761 taggctcgcg cgagttgctg gctgaggcgt tctcgaaatc agctcttgtt cggtcggcat
    11821 ctactctatt cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
    11881 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
    11941 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
    12001 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac
    12061 gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
    12121 tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa
    12181 tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg
    12241 acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
    12301 caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca
    12361 gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
    12421 tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc
    12481 gcagcgatcg catccatggc ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca
    12541 ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt caggctctcg
    12601 ctgaatgccc caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga
    12661 taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca
    12721 cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg
    12781 tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca
    12841 ggctttttca tatcttattg ccccccggga tcagatctgg attgagagtg aatatgagac
    12901 tctaattgga taccgagggg aatttatgga acgtcagtgg agcatttttg acaagaaata
    12961 tttgctagct gatagtgacc ttaggcgact tttgaacgcg caataatggt ttctgacgta
    13021 tgtgcttagc tcattaaact ccagaaaccc gcggctgagt ggctccttca acgttgcggt
    13081 tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt cataacgtga
    13141 ctcccttaat tctccgctca tgatcttgat cccctgcgcc atcagatcct tggcggcaag
    13201 aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc
    13261 aattccggtt cgcttgctgt ccataaaacc gcccagtcta gctatcgcca tgtaagccca
    13321 ctgcaagcta cctgctttct ctttgcgctt gcgttttccc ttgtccagat agcccagtag
    13381 ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctacgt gttccgcttc
    13441 ctttagcagc ccttgcgccc tgagtgcttg cggcagcgtg aagctgatcc gtcgagatta
    13501 atagaaataa attcagccta attcggcgtt aattcagtac attaaaaacg tccgcaatgt
    13561 gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag
    13621 ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc
    13681 agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc
    13741 gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg
    13801 agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca attcgggcac
    13861 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
    13921 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
    13981 tcatggcttg ttatgactgt tttttt
//
