LOCUS       sequence-450293-       13991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing Vitis riparia FLS2XL (FLS2 with eXtended Ligand 
            recognition) protein in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13991)
  TITLE     Coaker lab FLS2 plasmids
REFERENCE   2  (bases 1 to 13991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB514-35S:FLS2XL-HA
FEATURES             Location/Qualifiers
     source          1..13991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     489..508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     742..759
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    774..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1258..1452
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1518..2591)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3020..3649)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4950..4974
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     5162..5184
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5176..5193
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5177..5193
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5708..6053
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5996..6016
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    6075..6099
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9634..9658)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9687..9776
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      9810..10062
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(10095..10111)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10095..10111)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10108..10130)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10119..10135
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10143..10149,10150..10167,10168..10173))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10188..10209
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(10245..10497)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(10648..11673)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(11725..11904)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    12410..12434
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12955..13746
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaaagcta ccaactcttt ttccgaaggt aactggcttc
      121 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
      181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
      781 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg
     1081 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc
     1141 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc
     1201 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat
     1261 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa
     1321 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc
     1381 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac
     1441 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct
     1501 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag
     1561 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg
     1621 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg
     1681 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg
     1741 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga
     1801 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc
     1861 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc
     1921 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc
     1981 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt
     2041 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg
     2101 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc
     2161 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc
     2221 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc
     2281 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag
     2341 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc
     2401 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac
     2461 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc
     2521 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg
     2581 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga
     2641 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg
     2701 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc
     2761 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc
     2821 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc
     2881 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca
     2941 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt
     3001 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga
     3061 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag
     3121 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg
     3181 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa
     3241 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt
     3301 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg
     3361 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt
     3421 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg
     3481 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat
     3541 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg
     3601 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt
     3661 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt
     3721 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct
     3781 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac
     3841 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat
     3901 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc
     3961 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg
     4021 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg
     4081 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc
     4141 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca
     4201 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg
     4261 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg
     4321 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc
     4381 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc
     4441 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg
     4501 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt
     4561 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg
     4621 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga
     4681 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact
     4741 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact
     4801 gcactatagc agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt
     4861 tggcatgcac atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga
     4921 ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact
     4981 gatagtttaa actgaaggcg ggaaacgaca atctgatcca agctcaagct gctctagcat
     5041 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     5101 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     5161 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc ttgcatgcct gcaggtcccc
     5221 agattagcct tttcaatttc agaaagaatg ctaacccaca gatggttaga gaggcttacg
     5281 cagcaggtct catcaagacg atctacccga gcaataatct ccaggaaatc aaataccttc
     5341 ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga
     5401 aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc
     5461 acaaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttccc actgaatcaa
     5521 aggccatgga gtcaaagatt caaatagagg acctaacaga actcgccgta aagactggcg
     5581 aacagttcat acagagtctc ttacgactca atgacaagaa gaaaatcttc gtcaacatgg
     5641 tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa
     5701 gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc
     5761 cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc
     5821 atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag
     5881 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
     5941 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
     6001 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagaga acacggggga
     6061 ctctagagtt atcaacaagt ttgtacaaaa aagcaggctc cgcggccgcc cccttcacca
     6121 tggagcccag aagtgttggt ttaagagtag tggtagtttg ttctgttctg gttgtggtgg
     6181 ttataagcat ggatccaagt tttgaagtcg accatcaggc tttgaaggcc ttcaagagtt
     6241 cagttgctga cgatccctct ggagtgcttg cagattggag tgaagccaac catcattgca
     6301 attggtctgg aatcacatgt gacccttcat ccagtcgtgt catgtcaatt atactgatgg
     6361 agaagcagct tgccggcgtg atctcgccat tccttggaaa cctttccaaa ctccaggttc
     6421 tggacttaac tttaaacaca ttcactgggc aaattccacc tcaactgggg ctttgctccc
     6481 agctttcaga actgattctt taccaaaatt ctcttgccgg accaattcca caagaactag
     6541 gaatcctagg gaatttacaa tcattagatt tgggggctaa ctttttggaa ggaagcattc
     6601 ctgaaagcat ttgtaactgc actggcttgc tgaatctggg cattgataac aacaatctca
     6661 gtggtgccat cccttccgac attggaagat tagataatct tcaagtcttc acagggtaca
     6721 gaaataattt ggttggttct ataccagttt ccattggcac gttgggagct ttgcaggttc
     6781 tagacctgag tacaaaccat ttgtctggtg ttttacctcc agaaatgggg aacttgtcaa
     6841 accttgaaac ccttcagttg cttgaaaatc gacttcacgg gaaaatccca cctgaactag
     6901 ggctctgtag gaagctcacc actctgaatt tgtatggcaa tcagttcagt gggggcattc
     6961 cttcagaact aggaaacttg gttcatttga aagcgctgcg gttgtacaaa aataggttaa
     7021 gttctacaat tccatcctcc ttattccaat tgaaatcatt ggtccacttg ggaatatcag
     7081 agaatgaatt gagtggaaca atcccttttg aagttgggtc tctgagatca ttgcaagcac
     7141 tgacgctgca gttaaataag ttcactgggc aaatcccatc atccattaca aacttgacaa
     7201 acttaacata tttgtctatg gacttcaact tcttcacagg tgatattccg tccaacatcg
     7261 gatcgcttta tcgtttgaag aacctaaccc taaacaataa ccttctccag gggtccgttc
     7321 catctagtat ctctaactgc acacgtctag tcgtcttagg tctagcttat aatagaataa
     7381 caggaagaat tccacaaggt ttggggcggc ttgcaaatct tatttttcta tctttcggga
     7441 agaaccaaat gtcgggcaac attcctgatg atctcttcaa ttgctcaaat ctgattattc
     7501 tagatttggc caagaacaat ttcagtggag ttctgaaacc aggcattggc aagctctata
     7561 atctgcatat ttttcaagct cacaaaaatt ccttagtagg gcctatccca cctgagattg
     7621 gtaatctaag ccaactgttc tcattaaaac tacacttgaa tagtttgtcg ggcacagttc
     7681 ctcctgaatt atccaagctt tccctcctcc agggtctcta tctgaatgac aatgctctag
     7741 aaggtgcact tcctgaggta atttttgaac taaaacagct tagtgatctt gggttgggta
     7801 acaaccggtt tgcaggaccc attccacatg ctatctctaa gcttgagtcg cttttgtact
     7861 tgaccctcca tggaaacctg ttcaatggat ccattccaac gagcatggga cacctttctc
     7921 ggctggcaac cttggatctc tctcacaatc atctggtcgg gtcaattccg gggcctgtca
     7981 ttgctggtat gaaaaacatg caaatatatc tcaacttttc acacaatttc ctatcagggc
     8041 ccattccaaa tgagcttggg aaattggaga tggtgcagat cgtggatatg tcaaacaata
     8101 atctatcagg gagcattcca gcaactcttc aacgctgcaa aaacttgttc aatattgatc
     8161 tgtcagtgaa ccaactttcc ggtacaattc cagagaaggc atttgcggga atggatgtac
     8221 ttacaagctt gaatctttca agaaataatt tagatggtag actacctgga agcttagcga
     8281 ttatgaaaaa tctaagctcc ctggaccttt ctcagaacaa gttcaagggc atgatccccg
     8341 agagttatgc aaatatttcc acattgaggc atctgaatct ttctttcaat caactggaag
     8401 gtcatgtccc agcgactgga atattcaaaa atataggtgc atcttctctg gtgggaaacc
     8461 caggtctttg tggaaccaaa tttctcgggt catgcagcaa taagagccat ttagccggtt
     8521 ctcatccgtt ctccaagaaa gttctgttga ttctcggggt agttggatct ctcattgtat
     8581 tgctgcttct cacattttct gttataatct tctgtcggta cttcagaaag caaaaaacag
     8641 ttgaaaatcc agaaccagag tatgcttcag cattgacgct caagaggttc aatcaaaagg
     8701 atttagaaat agctactggt ttctttagtg aagaaaacat tattgggtcc agcagtttga
     8761 gtaccgtata caagggcaga atggaagatg ggaagattgt agccgtaaag aaattaaatt
     8821 tgcaacagtt ctcattggag tctgacaagt gcttcaatag agaagtgaag accctgagcc
     8881 aactcaggca cagaaacttg gtaaaggtac ttgggtatgc ctgggaaagt ggaaaaataa
     8941 aggctcttgt tctggaatac atggagaagg ggaacttgga cagcattata catgaaccag
     9001 gggtggatcc atcaaggtgg actttattgg agagaatcaa tgtttgtatt tccattgcaa
     9061 gggggctggt ttacttgcat tcagggtatg atttccccat cgtccactgt gacttgaagc
     9121 cttcaaatat tctcttggat ggagattggg aggcccatgt gagtgatttt ggaacagctc
     9181 gaattctggg tgttcatctc caggatggaa gcagtgtttg ctcatcatca gcatttgaag
     9241 gcacaattgg atacttggca ccagagttgg catacatgag ggagctcacg accaaagtgg
     9301 atgtgttcag ctttggcatc atagtgatgg agttcctgac aaaaagaagg ccaacaggac
     9361 tgacagcaga ggatggcatg ccattgacct tgcgtgagat ggtggatatg gcccttgcga
     9421 gtgaattcaa aaggcttctt caaatgatag acccttttct agcttcatct gcaactgaga
     9481 aggcggggga agtattggaa gaggtcctta aactggcctt gtcttgcacc tgcacagagc
     9541 ctgaggatcg acctgacatg aatgaggtgt tgtccagcct tttgaagcta ggagcaaagg
     9601 ttgcttcatc aacagcaaag ggtgggcgcg ccgacccagc tttcttgtac aaagtggttg
     9661 ataacagcgg gttaattaac atcttttacc catacgatgt tcctgactat gcgggctatc
     9721 cctatgacgt cccggactat gcaggatcct atccatatga cgttccagat tacgctgctc
     9781 agtgcagcgc ttagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct
     9841 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     9901 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     9961 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
    10021 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg gttccggaac
    10081 caattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    10141 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    10201 ctcacattag gctgaattag gcgcgcctat ttctattaat tcccgatcta gtaacataga
    10261 tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta
    10321 ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca
    10381 ttacatgtta attattacat gcttaacgta attcaacaga aattatatga taatcatcgc
    10441 aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg aacgatcggg
    10501 gaaattcggg ggatcgatcc cgcgaactgt ggacgagaac tgtgccacca agcgtaaggc
    10561 cgttctctcg catttgcctt gctaggctcg cgcgagttgc tggctgaggc gttctcgaaa
    10621 tcagctcttg ttcggtcggc atctactcta ttcctttgcc ctcggacgag tgctggggcg
    10681 tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct
    10741 gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg
    10801 accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc
    10861 aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct ccggatgcct
    10921 ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat
    10981 gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt
    11041 tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg
    11101 gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc
    11161 actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca
    11221 tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc
    11281 gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga ccggctgcag
    11341 aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga
    11401 gatgcaatag gtcaggctct cgctgaatgc cccaatgtca agcacttccg gaatcgggag
    11461 cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct
    11521 atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc
    11581 gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc
    11641 gacagacgtc gcggtgagtt caggcttttt catatcttat tgccccccgg gatcagatct
    11701 ggattgagag tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt
    11761 ggagcatttt tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg
    11821 cgcaataatg gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctga
    11881 gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc
    11941 atcggcgggg gtcataacgt gactccctta attctccgct catgatcttg atcccctgcg
    12001 ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt
    12061 accagagggc gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc
    12121 tagctatcgc catgtaagcc cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc
    12181 ccttgtccag atagcccagt agctgacatt catccggggt cagcaccgtt tctgcggact
    12241 ggctttctac gtgttccgct tcctttagca gcccttgcgc cctgagtgct tgcggcagcg
    12301 tgaagctgat ccgtcgagat taatagaaat aaattcagcc taattcggcg ttaattcagt
    12361 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac
    12421 aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac
    12481 cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc
    12541 ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt
    12601 tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc
    12661 tgcctgtgat caattcgggc acgaacccag tggacataag cctgttcggt tcgtaagctg
    12721 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
    12781 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
    12841 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
    12901 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgggg
    12961 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
    13021 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
    13081 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
    13141 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
    13201 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
    13261 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
    13321 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
    13381 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
    13441 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
    13501 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
    13561 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
    13621 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
    13681 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
    13741 aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc
    13801 aagcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct
    13861 tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgcatg
    13921 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    13981 aaaggatctt c
//
