LOCUS       sequence-45030-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Sheng Y, Laister RC, Lemak A, Wu B, Tai E, Duan S, Lukin J, 
            Sunnerhagen M, Srisailam S, Karra M, Benchimol S, Arrowsmith CH
  TITLE     Molecular basis of Pirh2-mediated p53 ubiquitylation.
  JOURNAL   Nat Struct Mol Biol. 2008 Dec . 15(12):1334-42.
  PUBMED    19043414
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2008 Dec . 15(12):1334-42."
COMMENT     Sequence Label: p53-(1-393)-E285A-N268D
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..79
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             89..106
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gggaaaattc cctctgaata ttttgtttac tttaagaagg agatatacca tgggcagcag
       61 ccatcatcat catcatcaca gcagcggcct ggtgccgcgc ggcagccata tggaggagcc
      121 gcagtcagat cctagcgtcg agccccctct gagtcaggaa acattttcag acctatggaa
      181 actacttcct gaaaacaacg ttctgtcccc cttgccgtcc caagcaatgg atgatttgat
      241 gctgtccccg gacgatattg aacaatggtt caccgaagac ccaggtccag atgaagctcc
      301 cagaatgcca gaggctgctc cccccgtggc ccctgcacca gcagctccta caccggcggc
      361 ccctgcacca gccccctcct ggcccctgtc atcttctgtc ccttcccaga aaacctacca
      421 gggcagctac ggtttccgtc tgggcttctt gcattctggg acagccaagt ctgtgacttg
      481 cacgtactcc cctgccctca acaagatgtt ttgccaactg gccaagacct gccctgtgca
      541 gctgtgggtt gattccacac ccccgcccgg cacccgcgtc cgcgccatgg ccatctacaa
      601 gcagtcacag cacatgacgg aggttgtgag gcgctgcccc caccatgagc gctgctcaga
      661 tagcgatggt ctggcccctc ctcagcatct tatccgagtg gaaggaaatt tgcgtgtgga
      721 gtatttggat gacagaaaca cttttcgaca tagtgtggtg gtgccctatg agccgcctga
      781 ggttggctct gactgtacca ccatccacta caactacatg tgtaacagtt cctgcatggg
      841 cggcatgaac cggaggccca tcctcaccat catcacactg gaagactcca gtggtaatct
      901 actgggacgg gacagctttg
//
