LOCUS       sequence-450310-       12678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of untagged full length human LRRK2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12678)
  AUTHORS   Dederer V, Sanz Murillo M, Karasmanis EP, Hatch KS, Chatterjee D, 
            Preuss F, Abdul Azeez KR, Nguyen LV, Galicia C, Dreier B, Pluckthun 
            A, Versees W, Mathea S, Leschziner AE, Reck-Peterson SL, Knapp S
  TITLE     A designed ankyrin-repeat protein that targets Parkinson's 
            disease-associated LRRK2.
  JOURNAL   J Biol Chem. 2024 Jul;300(7):107469. doi: 10.1016/j.jbc.2024.107469.
            Epub 2024 Jun 12.
  PUBMED    38876305
REFERENCE   2  (bases 1 to 12678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2024-07"; volume: "300(7):107469. doi"; pages: " 
            10.1016/j.jbc.2024.107469. Epub 2024 Jun 12"
COMMENT     Sequence Label: pCDNA5-LRRK2
FEATURES             Location/Qualifiers
     source          1..12678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(8630..8647)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8636..8860
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8993..9012)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    9144..9191
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             9199..10221
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             9199..10221
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    10351..10472
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10388..10407)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10442..10461
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10521..10537)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10521..10537)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10534..10556)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10545..10561
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10569..10575,10576..10593,10594..10599))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10614..10635
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10752..10769)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10923..11511)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11003..11022)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11682..12473,12474..12542))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12305..12324
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12543..12647)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc catggctagt ggcagctgtc
     1021 aggggtgcga agaggacgag gaaactctga agaagttgat agtcaggctg aacaatgtcc
     1081 aggaaggaaa acagatagaa acgctggtcc aaatcctgga ggatctgctg gtgttcacgt
     1141 actccgagca cgcctccaag ttatttcaag gcaaaaatat ccatgtgcct ctgttgatcg
     1201 tcttggactc ctatatgaga gtcgcgagtg tgcagcaggt gggttggtca cttctgtgca
     1261 aattaataga agtctgtcca ggtacaatgc aaagcttaat gggaccccag gatgttggaa
     1321 atgattggga agtccttggt gttcaccaat tgattcttaa aatgctaaca gttcataatg
     1381 ccagtgtaaa cttgtcagtg attggactga agaccttaga tctcctccta acttcaggta
     1441 aaatcacctt gctgatattg gatgaagaaa gtgatatttt catgttaatt tttgatgcca
     1501 tgcactcatt tccagccaat gatgaagtcc agaaacttgg atgcaaagct ttacatgtgc
     1561 tgtttgagag agtctcagag gagcaactga ctgaatttgt tgagaacaaa gattatatga
     1621 tattgttaag tgcgttaaca aattttaaag atgaagagga aattgtgctt catgtgctgc
     1681 attgtttaca ttccctagcg attccttgca ataatgtgga agtcctcatg agtggcaatg
     1741 tcaggtgtta taatattgtg gtggaagcta tgaaagcatt ccctatgagt gaaagaattc
     1801 aagaagtgag ttgctgtttg ctccataggc ttacattagg taattttttc aatatcctgg
     1861 tattaaacga agtccatgag tttgtggtga aagctgtgca gcagtaccca gagaatgcag
     1921 cattgcagat ctcagcgctc agctgtttgg ccctcctcac tgagactatt ttcttaaatc
     1981 aagatttaga ggaaaagaat gagaatcaag agaatgatga tgagggggaa gaagataaat
     2041 tgttttggct ggaagcctgt tacaaagcat taacgtggca tagaaagaac aagcacgtgc
     2101 aggaggccgc atgctgggca ctaaataatc tccttatgta ccaaaacagt ttacatgaga
     2161 agattggaga tgaagatggc catttcccag ctcataggga agtgatgctc tccatgctga
     2221 tgcattcttc atcaaaggaa gttttccagg catctgcgaa tgcattgtca actctcttag
     2281 aacaaaatgt taatttcaga aaaatactgt tatcaaaagg aatacacctg aatgttttgg
     2341 agttaatgca gaagcatata cattctcccg aagtggctga aagtggctgt aaaatgctaa
     2401 atcatctttt tgaaggaagc aacacttccc tggatataat ggcagcagtg gtccccaaaa
     2461 tactaacagt tatgaaacgt catgagacat cattaccagt gcagctggag gcgcttcgag
     2521 ctattttaca ttttatagtg cctggcatgc cagaagaatc cagggaggat acagaatttc
     2581 atcataagct aaatatggtt aaaaaacagt gtttcaagaa tgatattcac aaactggtcc
     2641 tagcagcttt gaacaggttc attggaaatc ctgggattca gaaatgtgga ttaaaagtaa
     2701 tttcttctat tgtacatttt cctgatgcat tagagatgtt atccctggaa ggtgctatgg
     2761 attcagtgct tcacacactg cagatgtatc cagatgacca agaaattcag tgtctgggtt
     2821 taagtcttat aggatacttg attacaaaga agaatgtgtt cataggaact ggacatctgc
     2881 tggcaaaaat tctggtttcc agcttatacc gatttaagga tgttgctgaa atacagacta
     2941 aaggatttca gacaatctta gcaatcctca aattgtcagc atctttttct aagctgctgg
     3001 tgcatcattc atttgactta gtaatattcc atcaaatgtc ttccaatatc atggaacaaa
     3061 aggatcaaca gtttctaaac ctctgttgca agtgttttgc aaaagtagct atggatgatt
     3121 acttaaaaaa tgtgatgcta gagagagcgt gtgatcagaa taacagcatc atggttgaat
     3181 gcttgcttct attgggagca gatgccaatc aagcaaagga gggatcttct ttaatttgtc
     3241 aggtatgtga gaaagagagc agtcccaaat tggtggaact cttactgaat agtggatctc
     3301 gtgaacaaga tgtacgaaaa gcgttgacga taagcattgg gaaaggtgac agccagatca
     3361 tcagcttgct cttaaggagg ctggccctgg atgttgccaa caatagcatt tgccttggag
     3421 gattttgtat aggaaaagtt gaaccttctt ggcttggtcc tttatttcca gataagactt
     3481 ctaatttaag gaaacaaaca aatatagcat ctacactagc aagaatggtg atcagatatc
     3541 agatgaaaag tgctgtggaa gaaggaacag cctcaggcag cgatggaaat ttttctgaag
     3601 atgtgctgtc taaatttgat gaatggacct ttattcctga ctcttctatg gacagtgtgt
     3661 ttgctcaaag tgatgacctg gatagtgaag gaagtgaagg ctcatttctt gtgaaaaaga
     3721 aatctaattc aattagtgta ggagaatttt accgagatgc cgtattacag cgttgctcac
     3781 caaatttgca aagacattcc aattccttgg ggcccatttt tgatcatgaa gatttactga
     3841 agcgaaaaag aaaaatatta tcttcagatg attcactcag gtcatcaaaa cttcaatccc
     3901 atatgaggca ttcagacagc atttcttctc tggcttctga gagagaatat attacatcac
     3961 tagacctttc agcaaatgaa ctaagagata ttgatgccct aagccagaaa tgctgtataa
     4021 gtgttcattt ggagcatctt gaaaagctgg agcttcacca gaatgcactc acgagctttc
     4081 cacaacagct atgtgaaact ctgaagagtt tgacacattt ggacttgcac agtaataaat
     4141 ttacatcatt tccttcttat ttgttgaaaa tgagttgtat tgctaatctt gatgtctctc
     4201 gaaatgacat tggaccctca gtggttttag atcctacagt gaaatgtcca actctgaaac
     4261 agtttaacct gtcatataac cagctgtctt ttgtacctga gaacctcact gatgtggtag
     4321 agaaactgga gcagctcatt ttagaaggaa ataaaatatc agggatatgc tcccccttga
     4381 gactgaagga actgaagatt ttaaacctta gtaagaacca catttcatcc ctatcagaga
     4441 actttcttga ggcttgtcct aaagtggaga gtttcagtgc cagaatgaat tttcttgctg
     4501 ctatgccttt cttgcctcct tctatgacaa tcctaaaatt atctcagaac aaattttcct
     4561 gtattccaga agcaatttta aatcttccac acttgcggtc tttagatatg agcagcaatg
     4621 atattcagta cctaccaggt cccgcacact ggaaatcttt gaacttaagg gaactcttat
     4681 ttagccataa tcagatcagc atcttggact tgagtgaaaa agcatattta tggtctagag
     4741 tagagaaact gcatctttct cacaataaac tgaaagagat tcctcctgag attggctgtc
     4801 ttgaaaatct gacatctctg gatgtcagtt acaacttgga actaagatcc tttcccaatg
     4861 aaatggggaa attaagcaaa atatgggatc ttcctttgga tgaactgcat cttaactttg
     4921 attttaaaca tataggatgt aaagccaaag acatcataag gtttcttcaa cagcgattaa
     4981 aaaaggctgt gccttataac cgaatgaaac ttatgattgt gggaaatact gggagtggta
     5041 aaaccacctt attgcagcaa ttaatgaaaa ccaagaaatc agatcttgga atgcaaagtg
     5101 ccacagttgg catagatgtg aaagactggc ctatccaaat aagagacaaa agaaagagag
     5161 atctcgtcct aaatgtgtgg gattttgcag gtcgtgagga attctatagt actcatcccc
     5221 attttatgac gcagcgagca ttgtaccttg ctgtctatga cctcagcaag ggacaggctg
     5281 aagttgatgc catgaagcct tggctcttca atataaaggc tcgcgcttct tcttcccctg
     5341 tgattctcgt tggcacacat ttggatgttt ctgatgagaa gcaacgcaaa gcctgcatga
     5401 gtaaaatcac caaggaactc ctgaataagc gagggttccc tgccatacga gattaccact
     5461 ttgtgaatgc caccgaggaa tctgatgctt tggcaaaact tcggaaaacc atcataaacg
     5521 agagccttaa tttcaagatc cgagatcagc ttgttgttgg acagctgatt ccagactgct
     5581 atgtagaact tgaaaaaatc attttatcgg agcgtaaaaa tgtgccaatt gaatttcccg
     5641 taattgaccg gaaacgatta ttacaactag tgagagaaaa tcagctgcag ttagatgaaa
     5701 atgagcttcc tcacgcagtt cactttctaa atgaatcagg agtccttctt cattttcaag
     5761 acccagcact gcagttaagt gacttgtact ttgtggaacc caagtggctt tgtaaaatca
     5821 tggcacagat tttgacagtg aaagtggaag gttgtccaaa acaccctaag ggaattattt
     5881 cgcgtagaga tgtggaaaaa tttctttcaa aaaaaaggaa atttccaaag aactacatgt
     5941 cacagtattt taagctccta gaaaaattcc agattgcttt gccaatagga gaagaatatt
     6001 tgctggttcc aagcagtttg tctgaccaca ggcctgtgat agagcttccc cattgtgaga
     6061 actctgaaat tatcatccga ctatatgaaa tgccttattt tccaatggga ttttggtcaa
     6121 gattaatcaa tcgattactt gagatttcac cttacatgct ttcagggaga gaacgagcac
     6181 ttcgcccaaa cagaatgtat tggcgacaag gcatttactt aaattggtct cctgaagctt
     6241 attgtctggt aggatctgaa gtcttagaca atcatccaga gagtttctta aaaattacag
     6301 ttccttcttg tagaaaaggc tgtattcttt tgggccaagt tgtggaccac attgattctc
     6361 tcatggaaga atggtttcct gggttgctgg agattgatat ttgtggtgaa ggagaaactc
     6421 tgttgaagaa atgggcatta tatagtttta atgatggcga agaacatcaa aaaatcttac
     6481 ttgatgactt gatgaagaaa gcagaggaag gagatctctt agtaaatcca gatcaaccaa
     6541 ggctcaccat tccaatatct cagattgccc ctgacttgat tttggctgac ctgcctagaa
     6601 atattatgtt gaataatgat gagttggaat ttgaacaagc tccagagttt ctcctaggtg
     6661 atggcagttt tggatcagtt taccgagcag cctatgaagg agaagaagtg gctgtgaaga
     6721 tttttaataa acatacatca ctcaggctgt taagacaaga gcttgtggtg ctttgccacc
     6781 tccaccaccc cagtttgata tctttgctgg cagctgggat tcgtccccgg atgttggtga
     6841 tggagttagc ctccaagggt tccttggatc gcctgcttca gcaggacaaa gccagcctca
     6901 ctagaaccct acagcacagg atcgcactcc acgtagctga tggtttgaga tacctccact
     6961 cagccatgat tatataccga gacctgaaac cccacaatgt gctgcttttc acactgtatc
     7021 ccaatgctgc catcatcgca aagattgctg actacggcat tgctcagtac tgctgtagaa
     7081 tggggataaa aacatcagag ggcacaccag ggtttcgtgc acctgaagtt gccagaggaa
     7141 atgtcattta taaccaacag gctgatgttt attcatttgg tttactactc tatgacattt
     7201 tgacaactgg aggtagaata gtagagggtt tgaagtttcc aaatgagttt gatgaattag
     7261 aaatacaagg aaaattacct gatccagtta aagaatatgg ttgtgcccca tggcctatgg
     7321 ttgagaaatt aattaaacag tgtttgaaag aaaatcctca agaaaggcct acttctgccc
     7381 aggtctttga cattttgaat tcagctgaat tagtctgtct gacgagacgc attttattac
     7441 ctaaaaacgt aattgttgaa tgcatggttg ctacacatca caacagcagg aatgcaagca
     7501 tttggctggg ctgtgggcac accgacagag gacagctctc atttcttgac ttaaatactg
     7561 aaggatacac ttctgaggaa gttgctgata gtagaatatt gtgcttagcc ttggtgcatc
     7621 ttcctgttga aaaggaaagc tggattgtgt ctgggacaca gtctggtact ctcctggtca
     7681 tcaataccga agatgggaaa aagagacata ccctagaaaa gatgactgat tctgtcactt
     7741 gtttgtattg caattccttt tccaagcaaa gcaaacaaaa aaattttctt ttggttggaa
     7801 ccgctgatgg caagttagca atttttgaag ataagactgt taagcttaaa ggagctgctc
     7861 ctttgaagat actaaatata ggaaatgtca gtactccatt gatgtgtttg agtgaatcca
     7921 caaattcaac ggaaagaaat gtaatgtggg gaggatgtgg cacaaagatt ttctcctttt
     7981 ctaatgattt caccattcag aaactcattg agacaagaac aagccaactg ttttcttatg
     8041 cagctttcag tgattccaac atcataacag tggtggtaga cactgctctc tatattgcta
     8101 agcaaaatag ccctgttgtg gaagtgtggg ataagaaaac tgaaaaactc tgtggactaa
     8161 tagactgcgt gcacttttta agggaggtaa tggtaaaaga aaacaaggaa tcaaaacaca
     8221 aaatgtctta ttctgggaga gtgaaaaccc tctgccttca gaagaacact gctctttgga
     8281 taggaactgg aggaggccat attttactcc tggatctttc aactcgtcga cttatacgtg
     8341 taatttacaa cttttgtaat tcggtcagag tcatgatgac agcacagcta ggaagcctta
     8401 aaaatgtcat gctggtattg ggctacaacc ggaaaaatac tgaaggtaca caaaagcaga
     8461 aagagataca atcttgcttg accgtttggg acatcaatct tccacatgaa gtgcaaaatt
     8521 tagaaaaaca cattgaagtg agaaaagaat tagctgaaaa aatgagacga acatctgttg
     8581 agtaagcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg
     8641 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     8701 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     8761 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     8821 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     8881 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     8941 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     9001 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     9061 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     9121 tagggtgatg gttcacgtac ctagaagttc ctattccgaa gttcctattc tctagaaagt
     9181 ataggaactt ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     9241 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     9301 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     9361 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     9421 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     9481 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     9541 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     9601 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     9661 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     9721 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     9781 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     9841 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     9901 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     9961 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
    10021 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
    10081 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
    10141 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
    10201 cgtccgaggg caaaggaata gcacgtacta cgagatttcg attccaccgc cgccttctat
    10261 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
    10321 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
    10381 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    10441 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
    10501 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    10561 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    10621 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    10681 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    10741 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    10801 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    10861 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    10921 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    10981 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    11041 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    11101 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    11161 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    11221 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    11281 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    11341 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    11401 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    11461 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    11521 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    11581 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    11641 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    11701 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    11761 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    11821 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    11881 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    11941 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    12001 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    12061 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    12121 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    12181 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    12241 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    12301 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    12361 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    12421 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    12481 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    12541 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    12601 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    12661 aaagtgccac ctgacgtc
//
