LOCUS       sequence-450317-       10366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Eukaryotic expression vector with E.Coli Dam methylation fused to 
            human Lamin B1. Used in DamID experiments to methylate DNA that 
            interacts in the proximity of Lamin B1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10366)
  AUTHORS   Guelen L, Pagie L, Brasset E, Meuleman W, Faza MB, Talhout W, Eussen
            BH, de Klein A, Wessels L, de Laat W, van Steensel B
  TITLE     Domain organization of human chromosomes revealed by mapping of 
            nuclear lamina interactions.
  JOURNAL   Nature. 2008 Jun 12;453(7197):948-51. doi: 10.1038/nature06947. Epub
            2008 May 7.
  PUBMED    18463634
REFERENCE   2  (bases 1 to 10366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature06947"; journalName: "Nature"; date: "2008-06-12- 
            12"; volume: "453"; issue: "7197"; pages: "948-51"
COMMENT     Sequence Label: pLenti GW V5 Eco/Dam hum LaminB1
FEATURES             Location/Qualifiers
     source          1..10366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(10..114)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(189..208)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        380..759
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        761..959
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     914..934
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             977..1157
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1204..1329
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1822..2055
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2240..2284
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2746..2984
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     2931..2952
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3879..3920
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    3936..3960
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5758..5782)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             6313..6493
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6519..6536)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6525..6749
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6795..7223
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6882..6901)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7092..7113
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7232..7252)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7237..7566
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7417..7552
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7479..7498
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7614..7661
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7680..8054
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8184..8305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8221..8240)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8275..8294
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8354..8370)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8354..8370)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8367..8389)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8378..8394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8402..8408,8409..8426,8427..8432))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8447..8468
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8585..8602)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8756..9344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8836..8855)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9515..10306,10307..10366,1..9))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10138..10157
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
       61 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
      121 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
      181 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
      241 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
      301 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
      361 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
      421 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      481 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      541 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      601 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      661 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      721 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      781 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      841 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      901 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc
      961 gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     1021 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     1081 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     1141 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     1201 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     1261 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     1321 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     1381 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     1441 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     1501 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     1561 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     1621 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     1681 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     1741 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     1801 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     1861 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     1921 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     1981 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     2041 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     2101 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     2161 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     2221 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     2281 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     2341 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     2401 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     2461 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     2521 agtgaacgga tcccccgggc tgcaggaatt cagatctcgg ccgcatatta agtgcattgt
     2581 tctcgatacc gctaagtgca ttgttctcgt tagctcgatg gacaagtgca ttgttctctt
     2641 gctgaaagct cgatggacaa gtgcattgtt ctcttgctga aagctcgatg gacaagtgca
     2701 ttgttctctt gctgaaagct cagtacccgg gagtaccctc gaccgccgga gtataaatag
     2761 aggcgcttcg tctacggagc gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa
     2821 gcgaaagcta agcaaataaa caagcgcagc tgaacaagct aaacaatctg cagtaaagtg
     2881 caagttaaag tgaatcaatt aaaagtaacc agcaaccaag taaatcaact gcaactactg
     2941 aaatctgcca agaagtaatt attgaataca agaagagaac tctgaatact ttcaacaagt
     3001 taccgagaaa gaagaactca cacacagcta gcgccaccat gaagaaaaat cgcgcttttt
     3061 tgaagtgggc agggggcaag tatcccctgc ttgatgatat taaacggcat ttgcccaagg
     3121 gcgaatgtct ggttgagcct tttgtaggtg ccgggtcggt gtttctcaac accgactttt
     3181 ctcgttatat ccttgccgat atcaatagcg acctgatcag tctctataac attgtgaaga
     3241 tgcgtactga tgagtacgta caggccgcac gcgagctgtt tgttcccgaa acaaattgcg
     3301 ccgaggttta ctatcagttc cgcgaagagt tcaacaaaag ccaggatccg ttccgtcggg
     3361 cggtactgtt tttatatttg aaccgctacg gttacaacgg cctgtgtcgt tacaatctgc
     3421 gcggtgagtt taacgtgccg ttcggccgct acaaaaaacc ctatttcccg gaagcagagt
     3481 tgtatcactt cgctgaaaaa gcgcagaatg cctttttcta ttgtgagtct tacgccgata
     3541 gcatggcgcg cgcagatgat gcatccgtcg tctattgcga tccgccttat gcaccgctgt
     3601 ctgcgaccgc caactttacg gcgtatcaca caaacagttt tacgcttgaa caacaagcgc
     3661 atctggcgga gatcgccgaa ggtctggttg agcgccatat tccagtgctg atctccaatc
     3721 acgatacgat gttaacgcgt gagtggtatc agcgcgcaaa attgcatgtc gtcaaagttc
     3781 gacgcagtat aagcagcaac ggcggcacac gtaaaaaggt ggacgaactg ctggctttgt
     3841 acaaaccagg agtcgtttca cccgcgaaaa aaaccggtgg taagcctatc cctaaccctc
     3901 tcctcggtct cgattctacg atcgatttcg atcaaacaag tttgtacaaa aaagcaggct
     3961 ccgcggccgc ccccttcacc atggcgactg cgacccccgt gccgccgcgg atgggcagcc
     4021 gcgctggcgg ccccaccacg ccgctgagcc ccacgcgcct gtcgcggctc caggagaagg
     4081 aggagctgcg cgagctcaat gaccggctgg cggtgtacat cgacaaggtg cgcagcctgg
     4141 agacggagaa cagcgcgctg cagctgcagg tgacggagcg cgaggaggtg cgcggccgtg
     4201 agctcaccgg cctcaaggcg ctctacgaga ccgagctggc cgacgcgcga cgcgcgctcg
     4261 acgacacggc ccgcgagcgc gccaagctgc agatcgagct gggcaagtgc aaggcggaac
     4321 acgaccagct gctcctcaac tatgctaaga aggaatctga tcttaatggc gcccagatca
     4381 agcttcgaga atatgaagca gcactgaatt cgaaagatgc agctcttgct actgcacttg
     4441 gtgacaaaaa aagtttagag ggagatttgg aggatctgaa ggatcagatt gcccagttgg
     4501 aagcctcctt agctgcagcc aaaaaacagt tagcagatga aactttactt aaagtagatt
     4561 tggagaatcg ttgtcagagc cttactgagg acttggagtt tcgcaaaagc atgtatgaag
     4621 aggagattaa cgagaccaga aggaagcatg aaacgcgctt ggtagaggtg gattctgggc
     4681 gtcaaattga gtatgagtac aagctggcgc aagcccttca tgagatgaga gagcaacatg
     4741 atgcccaagt gaggctgtat aaggaggagc tggagcagac ttaccatgcc aaacttgaga
     4801 atgccagact gtcatcagag atgaatactt ctactgtcaa cagtgccagg gaagaactga
     4861 tggaaagccg catgagaatt gagagccttt catcccagct ttctaatcta cagaaagagt
     4921 ctagagcatg tttggaaagg attcaagaat tagaggactt gcttgctaaa gaaaaagaca
     4981 actctcgtcg catgctgaca gacaaagaga gagagatggc ggaaataagg gatcaaatgc
     5041 agcaacagct gaatgactat gaacagcttc ttgatgtaaa gttagccctg gacatggaaa
     5101 tcagtgctta caggaaactc ttacaaggcg aagaagagag gttgaagctg tctccaagcc
     5161 cttcttcccg tgtgacagta tcccgagcat cctcaagtcg tagtgtacgt acaactagag
     5221 gaaagcggaa gagggttgat gtggaagaat cagaggcgag tagtagtgtt agcatctctc
     5281 attccgcctc agccactgga aatgtttgca tcgaagaaat tgatgttgat gggaaattta
     5341 tccgcttgaa gaacacttct gaacaggatc aaccaatggg aggctgggag atgatcagaa
     5401 aaattggaga cacatcagtc agttataaat atacctcaag atatgtgctg aaggcaggcc
     5461 agactgttac aatttgggct gcaaacgctg gtgtcacagc cagcccccca actgacctca
     5521 tctggaagaa ccagaactcg tggggcactg gcgaagatgt gaaggttata ttgaaaaatt
     5581 ctcagggaga ggaggttgct caaagaagta cagtctttaa aacaaccata cctgaagaag
     5641 aggaggagga ggaagaagca gctggagtgg ttgttgagga agaacttttc caccagcagg
     5701 gaaccccaag agcatccaat agaagctgtg caattatgta aaagggtggg cgcgccgacc
     5761 cagctttctt gtacaaagtg gttcgatcga accgcggtct agactcgaga cctagaaaaa
     5821 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     5881 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     5941 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     6001 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     6061 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     6121 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     6181 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     6241 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     6301 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6361 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6421 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6481 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     6541 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     6601 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     6661 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     6721 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     6781 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     6841 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     6901 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     6961 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7021 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7081 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7141 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     7201 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     7261 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7321 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7381 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7441 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7501 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     7561 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     7621 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     7681 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     7741 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     7801 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     7861 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     7921 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     7981 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     8041 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     8101 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     8161 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     8221 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8281 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     8341 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8401 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8461 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8521 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     8581 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8641 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8701 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8761 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8821 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8881 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8941 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9001 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9061 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9121 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9181 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9241 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9301 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9361 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9421 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9481 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9541 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9601 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9661 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9721 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9781 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9841 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9901 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9961 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    10021 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    10081 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    10141 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10201 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10261 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10321 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttg
//
