LOCUS       sequence-450337-        7619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 7619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pDESTmycIGF2BP3
FEATURES             Location/Qualifiers
     source          1..7619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        15..318
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        319..522
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     473..493
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     519..543
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             639..668
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    678..702
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2492..2516)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2548..2567)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2549..2567)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2578..2595)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2578..2594)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2587..2609)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    2999..3133
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3036..3055)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3090..3109
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3259..3714
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3346..3365)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3556..3577
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3831..4188
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3831..4022
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3854..3874)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4039..4174
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4239..5033
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4293..4312)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4903..4922
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5097..5145
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(5186..5205)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5305..5327
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        5451..5555
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5556..5624,5625..6416)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5774..5793)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6587..7175
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7076..7095
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7329..7346
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        complement(7420..7524)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cattagatgc atgtcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
       61 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      121 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      181 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      241 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      301 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      361 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      421 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      481 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
      541 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      601 agcctccgga ctctagccta ggccgcggta ccaccatgga acaaaaactt atttctgaag
      661 aagatctgtc tagatcaaca agtttgtaca aaaaagcagg ctccaccatg ggaaccaatt
      721 cagtcgacat gaacaaactg tatatcggaa acctcagcga gaacgccgcc ccctcggacc
      781 tagaaagtat cttcaaggac gccaagatcc cggtgtcggg acccttcctg gtgaagactg
      841 gctacgcgtt cgtggactgc ccggacgaga gctgggccct caaggccatc gaggcgcttt
      901 caggtaaaat agaactgcac gggaaaccca tagaagttga gcactcggtc ccaaaaaggc
      961 aaaggattcg gaaacttcag atacgaaata tcccgcctca tttacagtgg gaggtgctgg
     1021 atagtttact agtccagtat ggagtggtgg agagctgtga gcaagtgaac actgactcgg
     1081 aaactgcagt tgtaaatgta acctattcca gtaaggacca agctagacaa gcactagaca
     1141 aactgaatgg atttcagtta gagaatttca ccttgaaagt agcctatatc cctgatgaaa
     1201 tggccgccca gcaaaacccc ttgcagcagc cccgaggtcg ccgggggctt gggcagaggg
     1261 gctcctcaag gcaggggtct ccaggatccg tatccaagca gaaaccatgt gatttgcctc
     1321 tgcgcctgct ggttcccacc caatttgttg gagccatcat aggaaaagaa ggtgccacca
     1381 ttcggaacat caccaaacag acccagtcta aaatcgatgt ccaccgtaaa gaaaatgcgg
     1441 gggctgctga gaagtcgatt actatcctct ctactcctga aggcacctct gcggcttgta
     1501 agtctattct ggagattatg cataaggaag ctcaagatat aaaattcaca gaagagatcc
     1561 ccttgaagat tttagctcat aataactttg ttggacgtct tattggtaaa gaaggaagaa
     1621 atcttaaaaa aattgagcaa gacacagaca ctaaaatcac gatatctcca ttgcaggaat
     1681 tgacgctgta taatccagaa cgcactatta cagttaaagg caatgttgag acatgtgcca
     1741 aagctgagga ggagatcatg aagaaaatca gggagtctta tgaaaatgat attgcttcta
     1801 tgaatcttca agcacattta attcctggat taaatctgaa cgccttgggt ctgttcccac
     1861 ccacttcagg gatgccacct cccacctcag ggcccccttc agccatgact cctccctacc
     1921 cgcagtttga gcaatcagaa acggagactg ttcatctgtt tatcccagct ctatcagtcg
     1981 gtgccatcat cggcaagcag ggccagcaca tcaagcagct ttctcgcttt gctggagctt
     2041 caattaagat tgctccagca gaagcaccag atgctaaagt gaggatggtg attatcactg
     2101 gaccaccaga ggctcagttc aaggctcagg gaagaattta tggaaaaatt aaagaagaaa
     2161 actttgttag tcctaaagaa gaggtgaaac ttgaagctca tatcagagtg ccatcctttg
     2221 ctgctggcag agttattgga aaaggaggca aaacggtgaa tgaacttcag aatttgtcaa
     2281 gtgcagaagt tgttgtccct cgtgaccaga cacctgatga gaatgaccaa gtggttgtca
     2341 aaataactgg tcacttctat gcttgccagg ttgcccagag aaaaattcag gaaattctga
     2401 ctcaggtaaa gcagcaccaa caacagaagg ctctgcaaag tggaccacct cagtcaagac
     2461 ggaagtaagc ggccgcactc gagatatcta gacccagctt tcttgtacaa agtggttgat
     2521 cgcgtgcatg cgacgtcata gctctctccc tatagtgagt cgtattataa gctaggcact
     2581 ggccgtcgtt ttacaacgtc gtgactggga aaactgctag cttgggatct ttgtgaagga
     2641 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     2701 ggtaaatata aaatttttaa gtgtataatg tgttaaacta gctgcatatg cttgctgctt
     2761 gagagttttg cttactgagt atgatttatg aaaatattat acacaggagc tagtgattct
     2821 aattgtttgt gtattttaga ttcacagtcc caaggctcat ttcaggcccc tcagtcctca
     2881 cagtctgttc atgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     2941 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     3001 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3061 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     3121 tcatgtctgg atcgatcctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3181 gtattggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     3241 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3301 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     3361 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     3421 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     3481 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     3541 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     3601 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     3661 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctgat
     3721 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgcg gatctgcgca
     3781 gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
     3841 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     3901 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     3961 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4021 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4081 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     4141 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttgattct
     4201 tctgacacaa cagtctcgaa cttaaggcta gagccaccat gattgaacaa gatggattgc
     4261 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     4321 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     4381 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     4441 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     4501 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     4561 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     4621 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     4681 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     4741 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     4801 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     4861 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     4921 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     4981 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     5041 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgat ggccgcaata
     5101 aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagcgataa
     5161 ggatccgcgt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     5221 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5281 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5341 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5401 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5461 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5521 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5581 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5641 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5701 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5761 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5821 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5881 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5941 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6001 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6061 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6121 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6181 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6241 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6301 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6361 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6421 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6481 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6541 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6601 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6661 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6721 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6781 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6841 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6901 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6961 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7021 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7081 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7141 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7201 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7261 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     7321 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     7381 tccccgcgcg ttggccgatt cattaatgca gagcttgcaa ttcgcgcttt ttcaatatta
     7441 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7501 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     7561 aaccattatt atcatgacat taacctataa aaataggcgt attacgaggc cctttcacg
//
