LOCUS       sequence-45034-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Ohba T, Schirmer EC, Nishimoto T, Gerace L
  TITLE     Energy- and temperature-dependent transport of integral proteins to 
            the inner nuclear membrane via the nuclear pore.
  JOURNAL   J Cell Biol. 2004 Dec 20;167(6):1051-62.
  PUBMED    15611332
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2004-12-20"; volume: "167(6)"; pages: "1051-62"
COMMENT     Sequence Label: Reporter
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             102..368
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="MWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSF
                     NQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     regulatory      570..579
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(621..642)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttagtgaacc gtcagatccg ctagcgctac cggactcaga tctcgagctc aagcttcgaa
       61 ttctgcagac tatgcataga aggagatcca gatccagatc catgtggcat gaaggcctgg
      121 aagaggcatc tcgtttgtac tttggggaaa ggaacgtgaa aggcatgttt gaggtgctgg
      181 agcccttgca tgctatgatg gaacggggcc cccagactct gaaggaaaca tcctttaatc
      241 aggcctatgg tcgagattta atggaggccc aagagtggtg caggaagtac atgaaatcag
      301 ggaatgtcaa ggacctcacc caagcctggg acctctatta tcatgtgttc cgacgaatct
      361 caaagcaggt cgagggtggc ggtggcgagc tcagatccgt caagtcggaa aagacaaaga
      421 agggacgctc cgttcccatg tggataaaaa tgctgctgtt tgctctcgtg gcgggttttt
      481 tgtttttggt ctatcaagct atggaaacca accaaggaaa ccccttcact aattttcttc
      541 aagatactaa aatatccaac agatccatcg ccaccatggt gagcaagggc gaggagctgt
      601 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      661 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      721 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
      781 tgcagtgctt cagccgctac cccgaccaca tgaagcagca
//
