LOCUS       sequence-450355-        8342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing the SCT1 allele from UWOPS87-2421, under control 
            of its native promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Paltenghi C, van Leeuwen J
  TITLE     Genetic suppression interactions are highly conserved across 
            genetically diverse yeast isolates.
  JOURNAL   G3 (Bethesda). 2025 Mar 3:jkaf047. doi: 10.1093/g3journal/jkaf047.
  PUBMED    40037589
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2025 Mar 3:jkaf047. doi: 10.1093/g3journal/jkaf047."
COMMENT     Sequence Label: pRS313-SCT1-UWOPS872421
FEATURES             Location/Qualifiers
     source          1..8342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4534..4553)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4535..4553)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4560..4577)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4560..4576)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4569..4591)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4717..5172
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4804..4823)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5014..5035
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5432..6094)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(6095..6282)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(6429..6448)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6548..6570
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6608..6626)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6674..7177
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        7207..7311
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7312..7380,7381..8172)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7530..7549)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tggtaccggg ccccccctaa aaagcccctg tgcgagggat
     1081 atacataaac attgagcatt aatatttaca actcccaatg agcgataatg ggtttattac
     1141 ataaatagaa cggcgaaaga tttagtttct agtatcttca ccgtcagtaa atggcatcta
     1201 cgcatctcct tctttccctt cttcttcttc ttcctcttct tcttcctctt cctcttccct
     1261 ggcagtattt tcaccaattc tcttgtttaa gacggcctgt gcgattttac ttgctaatcc
     1321 atttttttcc tccaagattt cgttttctac ctcaaattcg gaggaagaag aaggtgcaac
     1381 ggatgtcgaa gctaaggaag actctgactt acgatgaaaa gtagaagaga ataatggaat
     1441 gttagagagg gaattgtcac tattgaccaa tgagacgcca tcggaatctt ggttatggtg
     1501 ttcataggca tcgtggttgt cacgttggct gatgataggt gatgacgaat ctttttcttg
     1561 tctttttatt ttctgttttc ttagcatttt cctgcgattc aattctgagg tttttcgatc
     1621 ttcttcctct tcatcgatga cgtcgaattc ttcacggagg gcggcactat cgaaatcggg
     1681 gaataattcg cttccaaagt tatttacaac ttcgattatt ctttctgcca gatttctacg
     1741 atccttttgt agcttttgca agccctttgg agatgtaaga gataaaacca gtggtctcaa
     1801 agatttgaaa ccatccatac caatatcatc cacgattaag gctgaatatg tgactataac
     1861 acacgagatg taagacccgg aaaaaacata tattttattc catggtttat gtctgaggta
     1921 ataagtgatt aaaacggacc aaaagatgta aagcaagggc gcaaatccca tcccaatcaa
     1981 gattttccac gtggcaatga catcattagc ctttatttta actgtagact tggacaaagc
     2041 ggtacgggcc ttttcttgag aaattctctt ggctagaatg aagacaggtg agaacataat
     2101 gatacctggc atggctaacg aaaagaggat gcagagcccg atggatctaa aaaaaacaag
     2161 tccgaggttt tttgcgaaat ttacctttgc ctcctccaca aggtgatcag gaagattata
     2221 gtgtctcaag gcggcattat atgccattat atctttggtc aagtccgcta tcttaggatc
     2281 gtttctatag aattcgtaac ctttgaccat tcttctgttc atttccacaa tcaagggcaa
     2341 cggtaacttg gtgctaaatt gtgtcatata tagtcttcct atcgtttgaa ccaccatcaa
     2401 agtttcataa tcagaacatg taacggtaac ggattgtaaa cccttcgata tggtatctaa
     2461 taactctttc actgcatctc tgttcgtttc cgggttgtgg tacttggcga ctagttcctt
     2521 cggtatttca atggggtcac caaattcaac aaccgctctc gacctgaact tatgtggatg
     2581 gaaataattc ataccacagg gaacaatctt aacattgacg tcaggatgct tatccatgca
     2641 accaagagcc ataatcgcca cacctgcttt caggggcaac aagtttgttc tgtcgtggga
     2701 cccaccttca ggaaagatcc caatgcagtt gttatgggcc aaatgctcaa aaactctatg
     2761 gtaaacgcaa gattggtcga ctttggcggc atatttataa gtagtgccgg tgagtaaggc
     2821 ggttttaatc tctggtttgg ccattttgaa ctcttttctt agggtcaaac tcgtgtcact
     2881 ttctatggac tggatttctc caaatcccat tgatttgggt aacccgatga gaccctttgg
     2941 catacaatca gtcaagaaat gcgtgtcgtg gccgataact ctcttgtagt ctgttggatc
     3001 tacgcggata gtaccttctg ccggtttcaa attatcctgc ggccttacca cgcctatggc
     3061 catgaagaaa ctagccaaaa accctatggc gggttgcttt aatgagctct ccgcaatcaa
     3121 gaaggacaca cgtctgttga cagatttctt cacctcgccc ataaggatta cagggtcgac
     3181 gaactggtta gcatgcggag ccgcaacaaa gatcaccggt ccctgttgcg gtaccttgaa
     3241 actgccccgg cctctgattt ctctaaagaa gcagtggaag atgttgtaca acagccatgt
     3301 ggcgatggag taaagtatct tcttggtagc gctaggctct tggtagatgt atgcctgatc
     3361 agccgacgtc ttgacgcttt tggcctcgat ggaactgtaa ttcgtagttt tctgtgtgct
     3421 cttggaagag gcagatttct ccgtgagttt tggtgcaggc attttttttt accccgatgt
     3481 aaacgcccct gtgctctttt tactatatat ttaattccgg gcttcctaca cttaattcac
     3541 tctgttatga actacaagcc attactaaca tcaatctttc cgaatcataa ataaactgca
     3601 actgggaaag aaggcgggcc tacttttctt tttcttcata gcgcgtaggg cttggcagcc
     3661 atagttgttg agagaccgat ggatgaggga ttgaaagaag ggagaagaag aatactaaaa
     3721 gaggaatgta gagccgggtt acaggaaaac taacttttca ctcagggcgc cgcatctttt
     3781 ccgcaggcgc gccatgaact ggccagcacg ttctacggcg aaaccttcga actggaggcg
     3841 gctcacacat agtagtaagt tgccgcgaca ggtgaccaaa atacaatctt taatatcatc
     3901 atatttgaac atgcccagtt tactgccatt atctagacgt gcgtctctca tcactttaca
     3961 ctcatgaaac aatattcgtt ttaatactga attttgagcg tcctttcttg ccgctgtcgc
     4021 ttctttgaac gcgtgcagag gaccgagcgt ttcgagtttg ttaagtagtt cggcggcaga
     4081 atcgcgcgcc caatgaatgc gtttatcttc tgttgttata cggtgggcgt aactgggttt
     4141 gtaagctgtt ggcgcttgca cacggttctg tggtagatat aacctctcac gaagcgttcg
     4201 gcccaaaagt tgggcgccaa gggcgcccaa ctggtccatc agtatggcag tcctccgagg
     4261 caggccctca gaattgaaat ctgccgtcga atcattgact ctcccttttg acagcattgt
     4321 tgcgatcgcc agtggctccg tctgcgctac aattgcacca tggtcaaacc gatccggatg
     4381 cagtgtctgt atagttacac cggtgtaagt gtcaccctcc agcagagctc gctggatagg
     4441 tgcactgcct ttatgtctgg gaagtagcga aggatggacg tttagcgccc actagttcta
     4501 gagcggccgc caccgcggtg gagctccaat tcgccctata gtgagtcgta ttacaattca
     4561 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     4621 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     4681 ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa
     4741 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4801 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4861 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4921 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4981 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5041 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     5101 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5161 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     5221 catagatccg tcgagttcaa gagaaaaaaa aagaaaaagc aaaaagaaaa aaggaaagcg
     5281 cgcctcgttc agaatgacac gtatagaatg atgcattacc ttgtcatctt cagtatcata
     5341 ctgttcgtat acatacttac tgacattcat aggtatacat atatacacat gtatatatat
     5401 cgtatgctgc agctttaaat aatcggtgtc actacataag aacacctttg gtggagggaa
     5461 catcgttggt accattgggc gaggtggctt ctcttatggc aaccgcaaga gccttgaacg
     5521 cactctcact acggtgatga tcattcttgc ctcgcagaca atcaacgtgg agggtaattc
     5581 tgctagcctc tgcaaagctt tcaagaaaat gcgggatcat ctcgcaagag agatctccta
     5641 ctttctccct ttgcaaacca agttcgacaa ctgcgtacgg cctgttcgaa agatctacca
     5701 ccgctctgga aagtgcctca tccaaaggcg caaatcctga tccaaacctt tttactccac
     5761 gcacggcccc tagggcctct ttaaaagctt gaccgagagc aatcccgcag tcttcagtgg
     5821 tgtgatggtc gtctatgtgt aagtcaccaa tgcactcaac gattagcgac cagccggaat
     5881 gcttggccag agcatgtatc atatggtcca gaaaccctat acctgtgtgg acgttaatca
     5941 cttgcgattg tgtggcctgt tctgctactg cttctgcctc tttttctggg aagatcgagt
     6001 gctctatcgc taggggacca ccctttaaag agatcgcaat ctgaatcttg gtttcatttg
     6061 taatacgctt tactagggct ttctgctctg tcatctttgc cttcgtttat cttgcctgct
     6121 cattttttag tatattcttc gaagaaatca cattacttta tataatgtat aattcattat
     6181 gtgataatgc caatcgctaa gaaaaaaaaa gagtcatccg ctaggtggaa aaaaaaaaat
     6241 gaaaatcatt accgaggcat aaaaaaatat agagtgtact agaggaggcc aagagtaata
     6301 gaaaaagaaa attgcgggaa aggactgtgt tatgacttcc ctgactaatg ccgtgttcaa
     6361 acgatacctg gcagtgactc ctagcgctca ccaagctctt aaaacgggaa tttatggtgc
     6421 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     6481 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     6541 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     6601 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     6661 tagggtcctt ttcatcacgt gctataaaaa taattataat ttaaattttt taatataaat
     6721 atataaatta aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt tttgttttcc
     6781 gaagatgtaa aagactctag ggggatcgcc aacaaatact accttttatc ttgctcttcc
     6841 tgctctcagg tattaatgcc gaattgtttc atcttgtctg tgtagaagac cacacacgaa
     6901 aatcctgtga ttttacattt tacttatcgt taatcgaatg tatatctatt taatctgctt
     6961 ttcttgtcta ataaatatat atgtaaagta cgctttttgt tgaaattttt taaacctttg
     7021 tttatttttt tttcttcatt ccgtaactct tctaccttct ttatttactt tctaaaatcc
     7081 aaatacaaaa cataaaaata aataaacaca gagtaaattc ccaaattatt ccatcattaa
     7141 aagatacgag gcgcgtgtaa gttacaggca agcgatccgt caggtggcac ttttcgggga
     7201 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7261 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     7321 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     7381 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     7441 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     7501 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     7561 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     7621 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     7681 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     7741 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     7801 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     7861 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     7921 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     7981 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8041 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8101 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8161 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8221 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     8281 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8341 tc
//
