LOCUS       sequence-450360-       10000 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing the SSD1 allele from NCYC110, under control of 
            its native promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Paltenghi C, van Leeuwen J
  TITLE     Genetic suppression interactions are highly conserved across 
            genetically diverse yeast isolates.
  JOURNAL   G3 (Bethesda). 2025 Mar 3:jkaf047. doi: 10.1093/g3journal/jkaf047.
  PUBMED    40037589
REFERENCE   2  (bases 1 to 10000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2025 Mar 3:jkaf047. doi: 10.1093/g3journal/jkaf047."
COMMENT     Sequence Label: pRS316-SSD1-NCYC110
FEATURES             Location/Qualifiers
     source          1..10000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6275..6294)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6276..6294)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6301..6318)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6301..6317)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6310..6332)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6458..6913
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6545..6564)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6755..6776
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7044..7847)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7848..8063)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(8087..8106)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8206..8228
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8266..8284)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    8325..8828
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        8865..8969
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8970..9038,9039..9830)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9188..9207)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tgggtaccgg gccccccccc agcgcataaa aagtaagctt
     1081 tcagtacttc tctctgatga tatttgtaca gcaaaacggt atttagtaac ggaataaatt
     1141 taatatataa gagtataaat aacaaaagac aatataaatt gaaaagttgt aaatattgaa
     1201 aagaaggctg cattattgtt ttatgtattt ttgggaaaaa atatatgact taacgaggtc
     1261 gaataaaaaa atttggatga tgccttagcg gaacgtggat taaaaggaga agtacaaaat
     1321 tcatgcagtt gttaatgttc gcaggaaata aaaacaaagg gaagggatac tgaggggtga
     1381 agcacaatcc aaagaacgaa agtgaaaaac aagaaaaaca gcaatgacga tattggtaga
     1441 agagattata ccctcttcat gaatggattt aatgcacgga cggttaaaca tggcaaagcc
     1501 atccccacct cagctctcaa tagaatagga atcttttgca gttcatgtat ttcttgtata
     1561 tagttatcat tttcaattct ggtttcagta gtagaaatga atttttctag aggattttgc
     1621 ttgttgtcgc ctgcagatgg tagccttaaa tttggtactg ttagatgcaa atcgctgagt
     1681 tccttactca atgggtcgct gatcaatggt tcagattccg cttctttatc tagttcaata
     1741 ttcgcaatct cagcggctgt ggatctgaat ttgttcttaa tggaatttct ataggatttt
     1801 ggattttttt catctgctgg tataaaagtt gcgctatcta cgccgggctg ccaatgcaat
     1861 tccaagacac gattggtacc atcaaactca gctttgatca aaggtagttg atctccatga
     1921 actctctttt caataccaaa ttctggaata aatacatcaa aggaggactc gtaaacttgt
     1981 aagacagtag ccattgttaa taattgtcct gtagtatttc ccatgtcgtt gattgtttta
     2041 cacaacaata gatgaattgc ttgttcctgt gcttgataag cacagtcctt tttaaaatta
     2101 caatattcgg aggtaatctt caaagcttcc atatcttcgg tgtatggagt atcgtggata
     2161 acggccttta attgcctatg aacgacatga tcagcgtatc ttctcattgg cgctgtgaaa
     2221 tgtgtgtaga taggtaggtt caaggcataa tgcccatatt ggtccgggtc tactttgcca
     2281 gcaataaagt atctagctct tggcatggtt ttaaacagta aaatttcaat tccgactcta
     2341 acatcgtcat ctttaatctt tagcacccct ttgattaatt catctgccgt attggtatca
     2401 aaattgtaac caaaattttg aattttcttt ctaaatgacg ccatcttggt tgcaatgggt
     2461 tgcatctgcc ttctcaaaag agctagatca ccaagtttgg tgtaaatttt ctctgcaaca
     2521 gtggagttga cctttctttt aatttcatta attacaacaa agcctaaagt tctatccagg
     2581 atgttcaagt caaccttaac tttttcgtca tccaagcttt ccaataagga cagggtggga
     2641 agtaatgtag cttctggatc atttattctt ctagcataaa atgatctagc aatttcctgt
     2701 acagtagaga ggtagctact gggacttcca gtagataatt tttcgtctaa ttgttctaaa
     2761 gacaagatat ttgaggggga aattgtagat tcgcctaccc aagtagattt aatccttaaa
     2821 gtagatgagt ctagagtgta aacaaccgac aacgtggctg attccttgcc aggggccaac
     2881 gacagttcgt cgttgaacga ttgtggtaat aaattgacaa gtttttgtgg catgaatacc
     2941 gcagaggacc tctttctcgc acgcctatca acagaggagc cttcttcaat atggctggtc
     3001 acatcaacaa catgacaacc cagctctagg gtaccatttc cgttattcct gacatgtaag
     3061 gcaaactcag acacccatcc aagctcggaa attgcaaaga tattgtactc attagtgtcc
     3121 ataaaattcc tcctatattc tagactttca gccgttaatg gtagcggctg aaaacttggt
     3181 ttttcttttt gcggattttt ttgatccaaa tattcattcg aaagaaaatt gttatccctt
     3241 aaaatggaat caatttcagt atcaggatcg tgaatatctc caagttcgga aactaaaata
     3301 ccaaatggat gcaaagatgt gattggccaa cgtttaatag aggcaacgaa taacttttcg
     3361 gagtatttat cagcgttttc aacaaagtcc tttggagcta attctgtagg aattgcaatt
     3421 aatgggacct tcttatcagt aggcttgaac caagcaattt ttgggctttg tggtggtttg
     3481 ttattgtcgc tattagcttg ttgggatggt ctcaacaaac ctaatgtacc gctaaataac
     3541 tgaccaggga tacggtccaa aacagcaacg acatggcctg cgtaaagtgg cttatattta
     3601 tcgttgattt cttcttcttc aactaataac aatgactgac cttcaacttc aacatcgtca
     3661 tttttctttt gagttggacg ttgtttcaat gaacttcttc tccttactcc tgatttggta
     3721 ggagatgata aggaatcact atcattattg atagtagatg acctctttct tctgacggat
     3781 aaatcatcct tgcttagcga atcagacgag gagggagaag atagaaaatt gttgcttgtt
     3841 gcagcagtaa cagatgcatc gttgtggtaa tcgtcactac tgttcaaagg aattagatcg
     3901 tgttgcatag aggcatcctt tctccttttc ttttcttcct tttctttctt ggactcccaa
     3961 acatcgtcca caactaatag ttctaccgcg actaaatcac cttcaagtgc tctattacga
     4021 tctttggagc cgcaaatgta aatatccgca tcaagagcgc catctgtaca gacccaagca
     4081 tccgatctat tctttttatt aactcttaat ataccagcta ctagtctccc ttcttggatt
     4141 agctctggaa tgttggcttg gggaaggtag ggtgcaaata gggacttacg tccacctccg
     4201 ttattattgc cttgtgaact gaaactatga actgatgaat tagaggcacg atgtttgtgt
     4261 ccctgctgaa atatcgcagg aggttctaag gtattgaagg aattgtaatc ccttgaatta
     4321 gatcctctgt ggcggaatgg agaaagctgc tgttgaggtt gctgtgattg gtttctccag
     4381 ttggaattaa tgcctggaga atattcattg tttcttgtct ttgaacggcg gtgcgttgaa
     4441 gaggttgcta tgaattcatc gtcattatcc ccgtgagagt ttggaggaaa tttgaaagag
     4501 ggaggtgata ggtttgaagt tgcgcgacga tggccctgat gagcatttgg tggtggtgga
     4561 aatttaaaat cagattgttc tgtagtagaa tttgagccac tatcagcttg aacagaatct
     4621 atcttcacag ttacgcctgc ttcaccccca gatattcttt tagcttgttc ttccgcagca
     4681 gcctttttcg cttcatttag acctaaagaa tgtcttctgg aatggccata tatgctggat
     4741 tgtgaactcg tctttctgtg tgagttagac ccaggattat tgctattgtt attgttatca
     4801 tatgaattat gataatatcc ggagttgttc ctggagtgag ttctacttct gctgccacct
     4861 gcactcatac caggtgaatt accgtttgag ttgtaatgag ggtgaggcgg ttgaggcgtg
     4921 aaatgattgt tagagccaga atttcctgac aggtattgtc ctgtctgttg tgccaattgc
     4981 tgttgctgag ccatcaactg ctgctgctgt gcttggactt gctccaactg cttctgcaaa
     5041 gtgaattgtt caatcatcaa atttgtcaac tcactttggg aacgtctgtg tgcaacatga
     5101 atctgctttg gggcattttt accacctcct gtggtttgca caaacatgtt atttggctct
     5161 tgggatctat tgttgttaac gttgctattt ttagacatag tgtataaaga tggaataatt
     5221 gggccaaatc tttgcgctga aaggtttttg gtgttctgtt ttgcgatatt aaagtgacaa
     5281 agaaaaagat ggaacttggc aaatactata cgtttatgaa cccttgtctt attcttcgtg
     5341 caggcaactt agatttccct ttgaaaaaaa gttgtttctt ttagaatagt ggctatgaaa
     5401 aaagtgcgcc taatgcggaa aaacgaggca ccagctgttc taaaataaga ccaaaatgtt
     5461 tagggtacca gaaaaatggt atggcgaaaa taagcctggc aagaagacta tgcagataac
     5521 tttgactatt ccggttttta ggttaccaaa gacagaagaa aggatcggtc ccaaaaaaag
     5581 gaaatagctt ataagggaag ttgcagaaaa tgcgcttgag taacaaaaaa tgttagaacg
     5641 tagcaaatat tctaaaagga atgacagaaa aacttcttac ctttctaaaa tataatctct
     5701 cctatttctc agctaggctc aaaattataa tttagaaatt attgtttgca cggcaaaact
     5761 tcgttccttt gctgttagaa aatcagtcgg tgttggactc ttcgtctttt aaattcttga
     5821 caagagaaga gtttttaaat gaaaagaact aacgagaaaa aaccagggga aggagggaaa
     5881 gagcgaaaat actcgtgatc taaactcttt taggttagtt ttcgtaggag gcagtggtct
     5941 taagacgatg gtgaaagaaa ggacttattt gtagaaggat cgtttattta atccttgaac
     6001 taaacaaatg acaaactcag gtctctagcg ttcctgttcg tggcctttaa taacttgatg
     6061 gcaatggtag aaagacacac ctgcgttata taagtttagt tttcccttct tttcctttct
     6121 catcccctcg tgaagcgact cggccccttt ggtcagaaaa ttcaaggtga tacaggtcgt
     6181 cgtccgtgac ataggatcag gaatcacagt catcacattg cactctcgtt cactagttct
     6241 agagcggccg ccaccgcggt ggagctccaa ttcgccctat agtgagtcgt attacaattc
     6301 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     6361 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     6421 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta
     6481 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6541 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6601 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6661 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6721 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6781 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6841 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6901 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     6961 gcatagggta ataactgata taattaaatt gaagctctaa tttgtgagtt tagtatacat
     7021 gcatttactt ataatacagt tttttagttt tgctggccgc atcttctcaa atatgcttcc
     7081 cagcctgctt ttctgtaacg ttcaccctct accttagcat cccttccctt tgcaaatagt
     7141 cctcttccaa caataataat gtcagatcct gtagagacca catcatccac ggttctatac
     7201 tgttgaccca atgcgtctcc cttgtcatct aaacccacac cgggtgtcat aatcaaccaa
     7261 tcgtaacctt catctcttcc acccatgtct ctttgagcaa taaagccgat aacaaaatct
     7321 ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt ctccagtaga tagggagccc
     7381 ttgcatgaca attctgctaa catcaaaagg cctctaggtt cctttgttac ttcttctgcc
     7441 gcctgcttca aaccgctaac aatacctggg cccaccacac cgtgtgcatt cgtaatgtct
     7501 gcccattctg ctattctgta tacacccgca gagtactgca atttgactgt attaccaatg
     7561 tcagcaaatt ttctgtcttc gaagagtaaa aaattgtact tggcggataa tgcctttagc
     7621 ggcttaactg tgccctccat ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa
     7681 caaattttgg gacctaatgc ttcaactaac tccagtaatt ccttggtggt acgaacatcc
     7741 aatgaagcac acaagtttgt ttgcttttcg tgcatgatat taaatagctt ggcagcaaca
     7801 ggactaggat gagtagcagc acgttcctta tatgtagctt tcgacatgat ttatcttcgt
     7861 ttcctgcagg tttttgttct gtgcagttgg gttaagaata ctgggcaatt tcatgtttct
     7921 tcaacactac atatgcgtat atataccaat ctaagtctgt gctccttcct tcgttcttcc
     7981 ttctgttcgg agattaccga atcaaaaaaa tttcaaggaa accgaaatca aaaaaaagaa
     8041 taaaaaaaaa atgatgaatt gaaaaggtgg tatggtgcac tctcagtaca atctgctctg
     8101 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     8161 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     8221 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     8281 tatttttata ggttaatgtc atgataataa tggtttctta gacggatcgc ttgcctgtaa
     8341 cttacacgcg cctcgtatct tttaatgatg gaataatttg ggaatttact ctgtgtttat
     8401 ttatttttat gttttgtatt tggattttag aaagtaaata aagaaggtag aagagttacg
     8461 gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc aacaaaaagc gtactttaca
     8521 tatatattta ttagacaaga aaagcagatt aaatagatat acattcgatt aacgataagt
     8581 aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac acagacaaga tgaaacaatt
     8641 cggcattaat acctgagagc aggaagagca agataaaagg tagtatttgt tggcgatccc
     8701 cctagagtct tttacatctt cggaaaacaa aaactatttt ttctttaatt tcttttttta
     8761 ctttctattt ttaatttata tatttatatt aaaaaattta aattataatt atttttatag
     8821 cacgtgatga aaaggaccca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     8881 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     8941 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     9001 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     9061 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     9121 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     9181 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     9241 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     9301 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     9361 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     9421 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     9481 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     9541 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     9601 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     9661 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     9721 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     9781 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     9841 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     9901 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9961 actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
