LOCUS       sequence-450362-        6930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express wild type igr3 (Rv3542c-Rv3541c-Rv3540c) in Mycobacteria 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Panagoda NT, Balazsi G, Sampson NS
  TITLE     Mycobacterium tuberculosis Mce3R TetR-like Repressor Forms an 
            Asymmetric Four-Helix Bundle and Binds a Nonpalindrome 
            Sequencedagger.
  JOURNAL   ACS Chem Biol. 2024 Dec 20;19(12):2580-2592. doi: 
            10.1021/acschembio.4c00687. Epub 2024 Nov 15.
  PUBMED    39545866
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.4c00687"; journalName: "ACS Chem Biol"; date: 
            "2024-12-20- 20"; volume: "19"; issue: "12"; pages: "2580-2592"
COMMENT     Sequence Label: pMV261-igr3WT
FEATURES             Location/Qualifiers
     source          1..6930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             561..590
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      3101..3147
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3297..3316)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3308..4123
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3381..3400)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4454..5042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4943..4962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttttgaata ccagccagac gagacggggt tctacgaatc ttggtcgata ccaagccatt
       61 tccgctgaat atcggggagc tcaccgccag aatcggtggt tgtggtgatg tacgtggcga
      121 actccgttgt agtgcctgtg gtggcatccg tggcgcggcc gcggtaccag atctttaaat
      181 ctagacggtg accacaacgc gcccgctttg atcggggacg tctgcggccg accatttacg
      241 ggtcttgttg tcgttggcgg tcatgggccg aacatactca cccggatcgg agggccgagg
      301 acaaggtcga acgaggggca tgacccggtg cggggcttct tgcactcggc ataggcgagt
      361 gctaagaata acgttggcac tcgcgaccgg tgagtgctag gtcgggacgg tgaggccagg
      421 cccgtcgtcg cagcgagtgg cagcgaggac aacttgagcc gtccgtcgcg ggcactgcgc
      481 ccggccagcg taagtagcgg ggttgccgtc acccggtgac ccccgtttca tccccgatcc
      541 ggaggaatca cttcgcaatg gaacaaaaac tcatctcaga agaggatctg gtgaccgggg
      601 tgagcgacat tcaggaagcg gttgcccaga tcaaggcggc cgggcccagc aagccgcgcc
      661 tggcccgtga cccggtgaac cagccgatga tcaacaactg ggtggaggcc atcggcgacc
      721 gcaaccccat ctatgtggac gacgcggcgg ctcgcgccgc cggccacccc ggaatcgtcg
      781 cgccaccggc catgattcag gtgtggacca tgatgggtct gggcggagtg cgtccgaagg
      841 acgatccgct gggacccatc atcaagctgt tcgacgacgc cggctacatc ggtgtggttg
      901 ccaccaactg cgagcagacc taccaccgct acctattgcc cggggagcaa gtcagcatca
      961 gcgccgagct cggtgacgtc gtcggaccca agcaaaccgc gctcggcgag ggctggttca
     1021 tcaaccagca catcgtatgg caagtcgggg acgaggacgt cgcagagatg aattggcgca
     1081 tcctcaaatt caagcccgcg ggttctccga gctcggtgcc tgacgatttg gatcccgacg
     1141 ctatgatgcg tccctcgtcg tcgcgggaca ccgcgttttt ctgggatgga gtcaaggccc
     1201 acgaattgcg gatccagcgg cttgccgatg gcagcctgcg gcacccaccg gtgccggcgg
     1261 tgtggcaaga caaatcggtg ccgatcaact acgtcgtctc cagcggtcgc ggcacggtgt
     1321 tcagcttcgt ggtgcatcac gcgccgaaag ttccgggtcg cacggtgccc ttcgtgatcg
     1381 cgctggtgga gctcgaggaa ggggtacgca tgctgggcga gctgcggggg gccgatcctg
     1441 ctcgtgttgc gatcggtatg ccggtacgag cgacctatat cgatttcccc gactggagcc
     1501 tctacgcatg ggagcctgac gaatgacggt tgttggtgct gtgctgcctg agctgaagct
     1561 ctacggagac ccgacgttca tcgtctcaac ggcgctggct accagggact tccaggatgt
     1621 gcatcatgat cgagacaagg ccgtcgccca ggggtcgaaa gacattttcg tcaacatcct
     1681 caccgacacc gggctggtgc aacgctacgt caccgactgg gcgggcccgt cggcgctgat
     1741 caagtcgatt gggctgcggc tgggggtgcc gtggtacgcc tacgacacgg tgacgttctc
     1801 cggtgaggtg accgcggtga acgacggctt gatcacggta aaggtggtgg gccgcaatac
     1861 ccttggcgac cacgtgaccg cgacggtgga actttcgatg agggattcgt agtgttatcg
     1921 ggtcaggcgg ccatcgtcgg tatcggcgcc accgactttt cgaagaactc cggtcgaagt
     1981 gagctgcggc tggcggccga ggcggtgttg gatgcgttgg ccgatgcggg cctgagcccg
     2041 accgatgtcg acgggctgac cacgttcacg atggacacca acaccgaaat cgccgtggcg
     2101 cgtgcggccg gcatcggcga gctgacgttc ttctccaaga tccactacgg cggtggcgcc
     2161 gcatgtgcga ccgtgcagca cgccgctatg gcagtggcca ccggggtggc tgacgtcgtg
     2221 gtggcgtatc gggcattcaa cgaacgatcc ggcatgcggt tcggtcaggt gcaaactcgt
     2281 ttgaccgaga atgccgactc caccggcgtg gacaattcgt tttcgtatcc gcacgggctc
     2341 tccacgcccg ccgcgcaagt ggcgatgatc gctcgccggt acatgcacct gtctggtgcg
     2401 accagccggg acttcggtgc tgtctcggtg gccgaccgca agcatgccgc caacaacccc
     2461 aaggcgtact tctacggcaa gccgataacc attgaggacc accagaattc gaggtggatc
     2521 gccgagccgc tgcggctgct ggactgctgc caggagaccg acggcgcggt cgcgatcgtg
     2581 gtgacgtcag ctgcgcgcgc acgggacctc aagcagcgcc cggtggtcat tgaggcggct
     2641 gcgcagggct gcagtccaga ccagtacacg atggtcagct actaccggcc ggaactcgac
     2701 ggcctgcccg agatgggcct ggtgggccgg cagctatggg cgcagtcggg gctgacgccg
     2761 gccgatgtcc agaccgcagt cctctacgac cacttcacgc cgtttaccct gattcagttg
     2821 gaggagttgg gattctgcgg caagggcgaa gcgaaagact tcatcgccga cggcgcgatc
     2881 gaggtgggcg ggcggctgcc catcaacacc cacggcggtc aactcggcga agcctacatc
     2941 catggcatga acggcatcgc ggagggggtg cggcagctgc gcggcacctc ggtgaacccg
     3001 gtggcgggcg tcgagcatgt gctcgtcacc gcgggcaccg gggtgcctac gtccgggctg
     3061 atcctgggtt aaaactagcg tacgatcgac tgccaggcat caaataaaac gaaaggctca
     3121 gtcgaaagac tgggcctttc gttttatgcc atcatggccg cggtgatcag ctagccacct
     3181 gacgtcgggg gggggggaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca
     3241 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     3301 gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat taaattccaa
     3361 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     3421 gacaatctat cgcttgtatg ggaagcccca tgcgccagag ttgtttctga aacatggcaa
     3481 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     3541 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     3601 cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     3661 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     3721 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     3781 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     3841 ctggaaagaa atgcataatc ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     3901 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     3961 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     4021 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     4081 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     4141 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcggctttg ttgaataaat
     4201 cgaacttttg ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc agaccgttcc
     4261 gtggcaaagc aaaagttcaa aatcaccaac tggtccacct acaacaaagc tctcaccaac
     4321 cgtggctccc tcactttctg gctggatgat ggggcgattc aggcctggta tgagtcagca
     4381 acaccttctt cacgaggcag acctcactag ttccatgagc gtcagacccc gtagaaaaga
     4441 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4501 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4561 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     4621 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4681 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4741 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4801 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4861 cgcttcccga ggggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4921 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4981 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5041 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5101 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5161 ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc gtgagcccac cagctccgta
     5221 agttcgggcg ctgtgtggct cgtacccgcg cattcaggcg gcagggggtc taacgggtct
     5281 aaggcggcgt gtacggccgc cacagcggct ctcagcggcc cggaaacgtc ctcgaaacga
     5341 cgcatgtgtt cctcctggtt ggtacaggtg gttgggggtg ctcggctgtc gctggtgttc
     5401 caccaccagg gctcgacggg agagcggggg agtgtgcagt tgtggggtgg cccctcagcg
     5461 aaatatctga cttggagctc gtgtcggacc atacaccggt gattaatcgt ggtctactac
     5521 caagcgtgag ccacgtcgcc gacgaatttg agcagctctg gctgccgtac tggccgctgg
     5581 caagcgacga tctgctcgag gggatctacc gccaaagccg cgcgtcggcc ctaggccgcc
     5641 ggtacatcga ggcgaaccca acagcgctgg caaacctgct ggtcgtggac gtagaccatc
     5701 cagacgcagc gctccgagcg ctcagcgccc gggggtccca tccgctgccc aacgcgatcg
     5761 tgggcaatcg cgccaacggc cacgcacacg cagtgtgggc actcaacgcc cctgttccac
     5821 gcaccgaata cgcgcggcgt aagccgctcg catacatggc ggcgtgcgcc gaaggccttc
     5881 ggcgcgccgt cgatggcgac cgcagttact caggcctcat gaccaaaaac cccggccaca
     5941 tcgcctggga aacggaatgg ctccactcag atctctacac actcagccac atcgaggccg
     6001 agctcggcgc gaacatgcca ccgccgcgct ggcgtcagca gaccacgtac aaagcggctc
     6061 cgacgccgct agggcggaat tgcgcactgt tcgattccgt caggttgtgg gcctatcgtc
     6121 ccgccctcat gcggatctac ctgccgaccc ggaacgtgga cggactcggc cgcgcgatct
     6181 atgccgagtg ccacgcgcga aacgccgaat ttccgtgcaa cgacgtgtgt cccggaccgc
     6241 taccggacag cgaggtccgc gccatcgcca acagcatttg gcgttggatc acaaccaagt
     6301 cgcgcatttg ggcggacggg atcgtggtct acgaggccac actcagtgcg cgccagtcgg
     6361 ccatctcgcg gaagggcgca gcagcgcgca cggcggcgag cacagttgcg cggcgcgcaa
     6421 agtccgcgtc agccatggag gcattgctat gagcgacggc tacagcgacg gctacaaccg
     6481 gcagccgact gtccgcaaaa agcggcgcgt gaccgccgcc gaaggcgctc gaatcaccgg
     6541 actatccgaa cgccacgtcg tccggctcgt ggcgcaggaa cgcagcgagt ggctcgccga
     6601 gcaggctgca cgccgcgaac gcatccgcgc ctatcacgac gacgagggcc actcttggcc
     6661 gcaaacggcc aaacatttcg ggctgcatct ggacaccgtt aagcgactcg gctatcgggc
     6721 gaggaaagag cgtgcggcag aacaggaagc ggctcaaaag gcccacaacg aagccgacaa
     6781 tccaccgctg ttctaacgca attggggagc gggtgtcgcg ggggttccgt ggggggttcc
     6841 gttgcaacgg gtcggacagg taaaagtcct ggtagacgct agttttctgg tttgggccat
     6901 gcctgtctcg ttgcgtgttt cgttgcgccc
//
