LOCUS       sequence-450372-        9734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express Mce3R mutant R53A in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9734)
  AUTHORS   Panagoda NT, Balazsi G, Sampson NS
  TITLE     Mycobacterium tuberculosis Mce3R TetR-like Repressor Forms an 
            Asymmetric Four-Helix Bundle and Binds a Nonpalindrome 
            Sequencedagger.
  JOURNAL   ACS Chem Biol. 2024 Dec 20;19(12):2580-2592. doi: 
            10.1021/acschembio.4c00687. Epub 2024 Nov 15.
  PUBMED    39545866
REFERENCE   2  (bases 1 to 9734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.4c00687"; journalName: "ACS Chem Biol"; date: 
            "2024-12-20- 20"; volume: "19"; issue: "12"; pages: "2580-2592"
COMMENT     Sequence Label: 2M-T-Mce3R_R53A
FEATURES             Location/Qualifiers
     source          1..9734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1192..1212)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(1231..2409)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1240..2178
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(1263..1283)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     repeat_region   2734..2768
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(2790..2808)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2829..2845)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2829..2845)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2842..2864)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2853..2869
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2877..2883,2884..2901,2902..2907))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2922..2943
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3060..3077)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3231..3819)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3311..3330)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3990..4781,4782..4850))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4613..4632
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4851..4955)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4981..5436)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5118..5139)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5330..5349
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5563..5585
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5577..5594
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5578..5594
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5601..5620
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5601..5619
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   5686..5748
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     5930..5949
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    6119..6707
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6225..6236
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6634..6654
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6723..7121)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(7122..7178)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(7188..7214)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(7224..7244)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(8019..9734,1..1182))
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
ORIGIN
        1 cttctcgctc agagacttct cgttgtcggg caggctctta ttggcggcag ccaccttggc
       61 atcctcctcg atatagtacc ggtagatcac aaagtccatc attgtataca ccttggttct
      121 gatagaatcg aacaccttgt ggttcttccg gatctcggac atatcctttc tgtccagcat
      181 cacttcccgc agctttgtga tgttaaagcc cagattcttc tgctccttca tgatgctgaa
      241 ccggaagtac tgctcggcga actcggcggg gttgatgccc agtgtctcgg cgatgtagtt
      301 cacattggcg gcgctgttct tggagaagct gtttgtcagc tcattggtga tccgatcata
      361 caggtagttc agtgtggaga tgtactcgtt atccagattc ttgtccagat tatacagcca
      421 ggtgcggctg atcctagact cctcctcgtt attgtgcacc acccagtgcc tcaggccgct
      481 cagcagggcc aggatgtcgt aacactcgtt gccgtaattg atgatatagt ttctgccctc
      541 cttagaaaag aaggcctggc caaaatagcc cagccggggg ttatccagaa aattatcgaa
      601 ctcgtcgtac tgggccttga tggcattgat cagcttgtcg ttattgttaa aggcggccct
      661 gtggtgctcg gggtccttga actcgtcgta ggtgtacacg gtagaaaact tgccgaagcc
      721 gatgatgtcc ttgtccaggc cggagatatt gttcacggcg taggcggcgt tggtgatata
      781 ctcggccagg atcttctcga tgtccaggat attgtggatg acctggatgc agatgttatc
      841 attgccgtcg gcgctctcgc cgaagtatct cttctccagt gtctccttca ggcccagcat
      901 gtcctgctgc actggtccgg tgtacagtgg attgttggcc accacggcat agccctttgg
      961 gtgggaaaac ttggcgttgc cgatcttata gccggcattc ttgtcggcca tctcggcgct
     1021 gaaggcctct ccctcgttca cggatctgat gctatcgccc tccacgatct tctccagccg
     1081 tgcgtcggag ccctcggcaa atgtggtcat gtacacctta gagccggaca ccagggtgct
     1141 cttcacgccc atgcccttgg cgaagctctt cttcttctcg atgctggcct ccacctttct
     1201 cttcttcttg gggctcatgg tggccgtacg tcacgacacc tgaaatggaa gaaaaaaact
     1261 ttgaaccact gtctgaggct tgagaatgaa ccaagatcca aactcaaaaa gggcaaattc
     1321 caaggagaat tacatcaagt gccaagctgg cctaacttca gtctccaccc actcagtgtg
     1381 gggaaactcc atcgcataaa acccctcccc ccaacctaaa gacgacgtac tccaaaagct
     1441 cgagaactaa tcgaggtgcc tggacggcgc ccggtactcc gtggagtcac atgaagcgac
     1501 ggctgaggac ggaaaggccc ttttcctttg tgtgggtgac tcacccgccc gctctcccga
     1561 gcgccgcgtc ctccattttg agctccctgc agcagggccg ggaagcggcc atctttccgc
     1621 tcacgcaact ggtgccgacc gggccagcct tgccgcccag ggcggggcga tacacggcgg
     1681 cgcgaggcca ggcaccagag caggccggcc agcttgagac tacccccgtc cgattctcgg
     1741 tggccgcgct cgcaggcccc gcctcgccga acatgtgcgc tgggacgcac gggccccgtc
     1801 gccgcccgcg gccccaaaaa ccgaaatacc agtgtgcaga tcttggcccg catttacaag
     1861 actatcttgc cagaaaaaaa gcgtcgcagc aggtcatcaa aaattttaaa tggctagaga
     1921 cttatcgaaa gcagcgagac aggcgcgaag gtgccaccag attcgcacgc ggcggcccca
     1981 gcgcccaggc caggcctcaa ctcaagcacg aggcgaaggg gctccttaag cgcaaggcct
     2041 cgaactctcc cacccacttc caacccgaag ctcgggatca agaatcacgt actgcagcca
     2101 ggtggaagta attcaaggca cgcaagggcc ataacccgta aagaggccag gcccgcggga
     2161 accacacacg gcacttacct gtgttctggc ggcaaacccg ttgcgaaaaa gaacgttcac
     2221 ggcgactact gcacttatat acggttctcc cccaccctcg ggaaaaaggc ggagccagta
     2281 cacgacatca ctttcccagt ttaccccgcg ccaccttctc taggcaccgg ttcaattgcc
     2341 gacccctccc cccaacttct cggggactgt gggcgatgtg cgctctgccc actgacgggc
     2401 accggagcca attcccactc ctttcaagac ctagaaggtc cattagctgc aaagattccg
     2461 atatctataa caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa
     2521 caatataatt atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat
     2581 tttgactcac gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg
     2641 cctcacggga gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc
     2701 tatttagaaa gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct
     2761 ttctagggtt aagtacccag cttttgttcc ctttagtgag ggttaatttc gagcttggcg
     2821 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     2881 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     2941 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     3001 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     3061 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     3121 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3181 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3241 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3301 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3361 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3421 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3481 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3541 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3601 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3661 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3721 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3781 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3841 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3901 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3961 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4021 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4081 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     4141 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4201 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4261 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg
     4321 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4381 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4441 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4501 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4561 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     4621 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4681 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4741 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4801 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4861 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4921 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4981 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     5041 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     5101 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     5161 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     5221 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     5281 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     5341 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     5401 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
     5461 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     5521 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     5581 aaacgacggc cagtgaattg taatacgact cactataggg cgaattggag ctcggtattc
     5641 acgacagcag gctgaataat aaaaaaatta gaaactatta tttaacccta gaaagataat
     5701 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
     5761 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
     5821 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
     5881 aacaaaaact caaaatttct tctataaagt aacaaaagct agcatttgtg aaatttgtga
     5941 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     6001 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     6061 cctctacaaa tgtggtatgg ctgattatgc cttgtaagtc attggtctta aagtcgacgc
     6121 ggggaggcgg cccaaaggga aatccgactc gtctgagggc gaaggcgaag acgcggaaga
     6181 ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg
     6241 acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccaagga
     6301 aaggacgatg atttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct
     6361 gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa
     6421 gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg
     6481 tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa
     6541 agagacagca accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata
     6601 gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta
     6661 agaataccag tcaatctttc acaaattttg taatccagag gttgattgtc gacttaacgc
     6721 gtttagccct cccacacata accagagggc agcaattcac gaatcccaac tgccgtcggc
     6781 tgtccatcac tgtccttcac tatggctttg atcccaggat gcagatcgag aagcacctgt
     6841 cggcaccgtc cgcaggggct caagatgccc ctgttctcat ttccgatcgc gacgatacaa
     6901 gtcaggttgc cagctgccgc agcagcagca gtgcccagca ccacgagttc tgcacaaggt
     6961 cccccagtaa aatgatatac attgacacca gtgaagatgc ggccgtcgct agagagagct
     7021 gcgctggcga cgctgtagtc ttcagagatg gggatgctgt tgattgtagc cgttgctctt
     7081 tcaatgaggg tggattcttc ttgagacaaa ggcttggcca tcggtccagg attctcttcg
     7141 acatctccgg cttgtttcag cagagagaag tttgttgcgc cggatccagc gtaatctgga
     7201 acatcgtatg ggtaagcggc cgccaccttc ctctttttct taggtccgga tcccttgtac
     7261 agctcatcca taccgagagt gatgcctgcg gcagtcacaa attccagaag gaccatgtga
     7321 tctctctttt cgtttgggtc cttggaaagc tttgattgcg tggagagata atgattgtca
     7381 ggcaagagta caggtccatc tccgatgggc gtattttgtt ggtaatgatc agccagttga
     7441 acactaccat cttcgacatt atgtcggatc ttaaagttgg cctttatccc atttttctgc
     7501 ttatcagcgg taatatacac attatggctg ttaaagttgt actccaactt gtgccccagt
     7561 atgttaccgt cctccttaaa atcaatacct ttcagttcaa ttcgattaac cagagtgtcc
     7621 ccttcaaatt taacctcggc tcgggtttta tatgtaccat catccttaaa gctgatggtc
     7681 ctttcttgaa cataaccttc aggcattgca gacttgaaaa agtcatgctg tttcatgtgg
     7741 tcgggatatc gagaaaagca ttgcacgccg taggtgaggg tcgttacgag tgtaggccat
     7801 gggacgggca gtttgcccgt cgtacagatg aatttcagag tcagcttacc attcgtggcg
     7861 tccccttcgc cttcgcccct gacggagaat ttatgtccat ttacatctcc atcaagctct
     7921 accagaatgg gcactactcc agtgaagagt tcttcacctt tactcacgct acctcctcct
     7981 ccgcttcctc ctcctccgct tccacctcct cctctagagg agttgccgct caccttcttc
     8041 ttctccttat caaacttggc tgcctcgttc ctgtcgaaca gggcctcgat gctcagattc
     8101 ttaaaccttg ggatacagta gccgaaaggc acgcacagca gcttcagcag tctatcgtta
     8161 tacttcttct catcattcac ggcgtcgaag tactcagaca ccttgccgct agacttctca
     8221 tatctctcgt tcatgatgat ccgctgcatg atatagtggt acagctgaaa gtaggagttc
     8281 acctcggcga tgtcattgat gtaggcgtgc acatagcggg ccacctccag gtgcacggcc
     8341 ttgttcctga acagtgtaca ggtcttatta tcgatgcgca gcaggtcctc cctgatgatc
     8401 acgttccact ttgtattccg caggtaggcg ttcagggcgg tcttggcctt ctctctattg
     8461 atctgccggg tgaactcctc ggccttcttc tcatcggagt acttgatata gttggcgtac
     8521 agcttaggat tggcagactc cagggagtcg atgctctcct tggctctctc ggccatctcc
     8581 ttctccacat ccttccgctt gtcgggtgct gtctcgtcga ttcctgcgca cagcttggtc
     8641 acggagctaa agcccttctc ctccagcttc ttcaggttga tgtcgtagcc cttctcctta
     8701 tacagctggg catcgcgctc cacgcagtgg aagccgatca cgtatctggc gttgatattc
     8761 acgatattct tcaggatgtg gtagatcact gtcagataca ggctgatgat cttcttaaac
     8821 ttctctctct ctgcgttctc ccttccggtg tcctcgatca cgctccgctt cttatcgaac
     8881 tggtcataat tcatgcctgt gatgatcttg gtcagggcgt ccaccttctc agacacggac
     8941 ttgcccttat ccttgccgat gcatgtctca tagtagcggt cgatctggtt cttgccattc
     9001 tggccctgct tcttctggat gtcggcgatc ctgcccagca cgaacttcac cacggcctcg
     9061 ttcttggcga tctcgtgcag gtgtgcaggg tcgccgtatc tgatcagata gtgaaacctc
     9121 ttgttgctga tcacgttatt gatgatgaaa ttgcgcatgc cgtgcttgcc cttcttcagc
     9181 ttgttgccat tctcgtccag gctgaaggtg tcggccaggg ccttcagctc atcataagac
     9241 agattggtgc ccaggattct gatggcatcg atgtacatgg ctctccttgc gtctgcgatt
     9301 ggctctccca tcctggcaaa ggacttgatc agcctcagct catcggcgat cttggcgctg
     9361 tccttaaaga aggcgtactc ctccacgaac ttggcgttca cgccgatcag aggcatcact
     9421 ttcagaaagg actggatgtt gtcgaactta ttgatcagtg tggtcagcag atcattgatc
     9481 tccttgccgt ccagaaacat tgtcagggca tacatcagct tgctgaaggc gctcacgtcc
     9541 cttcctgcag gcaggatgcg aggcagcctt ggtgcgtcct tcttcttgta ctcgcgggtc
     9601 ttgttgcccc tgaactcctt gatgttgtgc atgatattct ccagctttct ccagatccga
     9661 ttggcctcat catagtacag ggcgtccttc tgatcgtcgt taaaggagcc tctcagattg
     9721 atcacgaaga tgtc
//
