LOCUS       sequence-450375-        9084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression of mCherry-labeled Rab25..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9084)
  TITLE     Harper lab Rab11 and inflammasome reporter plasmids
REFERENCE   2  (bases 1 to 9084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE mCherry-Rab25
FEATURES             Location/Qualifiers
     source          1..9084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1000..1021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1036..1041,1042..1059,1060..1066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1074..1090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1079..1101
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1098..1114
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1098..1114
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1164..1183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1375..2008
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2055..2180
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2677..2910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3095..3139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3436..3553
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3605..3908
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3909..4112
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4063..4083
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4109..4133
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4215..4922
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4360..4378)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4628..4647
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    4947..4971
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5614..5638)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5651..6218
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5668..6220
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5812..5829)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6039..6058
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6232..6831
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6232..6251)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6728..6748
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6848..7436
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6901..6921)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7319..7330)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7511..7744
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7863..7881)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7949..8053
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8054..8122,8123..8914)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8272..8291)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
      841 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      901 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
      961 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1021 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1081 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
     1141 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     1201 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     1261 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     1321 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
     1381 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     1441 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     1501 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     1561 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     1621 aagtgttaga gtgtaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     1681 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     1741 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     1801 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     1861 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     1921 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     1981 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     2041 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     2101 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     2161 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     2221 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     2281 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     2341 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     2401 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     2461 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     2521 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     2581 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     2641 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     2701 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     2761 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     2821 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     2881 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     2941 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     3001 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     3061 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     3121 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     3181 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     3241 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     3301 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     3361 cagatccatt cgattagtga acggatctcg acggtatcgc cgaattaatt cacaaatggc
     3421 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     3481 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     3541 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga ctagtggagt
     3601 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     3661 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     3721 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     3781 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     3841 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     3901 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     3961 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     4021 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     4081 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
     4141 gacgccatcc acgctgtttt gacctccata gaagacaccg gcggccgcag atctctcgag
     4201 gttgatctgc tagcatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
     4261 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     4321 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     4381 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     4441 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     4501 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     4561 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     4621 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     4681 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     4741 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     4801 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     4861 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     4921 aggcatgcag cgctagtgct tatcaaacaa gtttgtacaa aaaagttggc atggggaatg
     4981 gaactgagga agattataac tttgtcttca aggtggtgct gatcggcgaa tcaggtgtgg
     5041 ggaagaccaa tctactctcc cgattcacgc gcaatgagtt cagccacgac agccgcacca
     5101 ccatcggggt tgagttctcc acccgcactg tgatgttggg caccgctgct gtcaaggctc
     5161 agatctggga cacagctggc ctggagcggt accgagccat cacctcggcg tactatcgtg
     5221 gtgcagtggg ggccctcctg gtgtttgacc taaccaagca ccagacctat gctgtggtgg
     5281 agcgatggct gaaggagctc tatgaccatg ctgaagccac gatcgtcgtc atgctcgtgg
     5341 gtaacaaaag tgacctcagc caggcccggg aagtgcccac tgaggaggcc cgaatgttcg
     5401 ctgaaaacaa tggactgctc ttcctggaga cctcagccct ggactctacc aatgttgagc
     5461 tagcctttga gactgtcctg aaagaaatct ttgcgaaggt gtccaagcag agacagaaca
     5521 gcatccggac caatgccatc actctgggca gtgcccaggc tggacaggag cctggccctg
     5581 gggagaagag ggcctgttgc atcagcctct aatgcccaac tttcttgtac aaagtggttc
     5641 gataacgaat tccgcccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     5701 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     5761 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     5821 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     5881 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     5941 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     6001 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     6061 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     6121 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     6181 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catgaccgag
     6241 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     6301 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     6361 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     6421 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     6481 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     6541 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     6601 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     6661 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     6721 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     6781 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg atgcatcgat
     6841 ggatcctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6901 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6961 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     7021 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7081 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7141 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7201 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     7261 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7321 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7381 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga
     7441 gatcctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     7501 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     7561 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7621 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7681 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7741 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7801 tcagagagtg agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa
     7861 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     7921 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7981 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     8041 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     8101 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     8161 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     8221 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     8281 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     8341 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     8401 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     8461 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     8521 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     8581 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     8641 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     8701 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8761 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8821 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8881 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8941 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     9001 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9061 cgtagaaaag atcaaaggat cttc
//
