LOCUS       sequence-450412-        8287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor RORA with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8287)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 8287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-RORA-Brd 206
FEATURES             Location/Qualifiers
     source          1..8287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1809..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1960..2366
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    4291..4694
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             4813..5046
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5118..5239
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5155..5174)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5209..5228
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5279..5414
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5434..5453)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5435..5453)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5463..5480)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5463..5479)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5472..5494)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5621..6076
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5708..5727)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5918..5939
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6102..6206
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6207..6275,6276..7067)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6425..6444)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7238..7826
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7727..7746
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7980..7997
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8114..8135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8150..8155,8156..8173,8174..8180)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8188..8204
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8193..8215
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8212..8228
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8212..8228
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8247..8267
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8249..8267
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1921 aattttatcg atcacgagac tagcctcgag ctggctgcag taacgccatt ttgcaaggca
     1981 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2041 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2101 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2161 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2221 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2281 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2341 gtcctccgac agactgagtc ggccggtcga atcaagctta tcgataccgt cgacgccacc
     2401 atgaatgaag gcgcccctgg cgacagcgac ctggaaacag aagctagagt cccctggtcc
     2461 atcatgggcc actgtctgag aacaggccag gccagaatga gcgccacacc tacaccagct
     2521 ggcgaaggcg ccagaagaga tgagctgttt ggcatcctcc agatcctgca ccagtgcatc
     2581 ctgagcagcg gagatgcctt tgtgctgacc ggcgtgtgct gctcttggag acagaatggc
     2641 aagcctcctt acagccagaa agaggacaaa gaggtccaga ccggctacat gaacgcccag
     2701 atcgagatca tcccctgcaa gatctgcggc gacaagtcca gcggaatcca ctacggcgtg
     2761 atcacatgcg agggctgcaa gggcttcttc agaagatccc agcagagcaa cgccacctac
     2821 agctgcccca gacagaagaa ctgcctgatc gaccggacca gccggaacag atgccagcac
     2881 tgtagactcc agaaatgcct ggccgtgggc atgagcagag atgccgtgaa gttcggccgg
     2941 atgagcaaga agcagcggga tagcctgtac gccgaggtgc agaaacaccg gatgcagcag
     3001 cagcaaaggg accaccaaca gcagccaggc gaagccgaac ctctgacacc cacctacaac
     3061 atcagcgcca atggcctgac cgagctgcac gacgacctga gcaactacat cgacggccac
     3121 acacctgagg gcagcaaagc cgattctgcc gtgtccagct tctacctgga catccagcct
     3181 tctccagacc agagcggcct ggatatcaac ggcatcaagc ccgagcctat ctgcgactat
     3241 acccctgcca gcggattctt cccctactgc tccttcacca acggcgagac aagccccaca
     3301 gtgtctatgg ccgagctgga acatctggcc cagaacatca gcaagagcca tctggaaacc
     3361 tgccagtacc tgagagagga actccagcag attacctggc agaccttcct gcaagaggaa
     3421 atcgagaact accagaacaa gcagcgcgaa gtgatgtggc agctgtgcgc catcaagatc
     3481 accgaggcca tccagtacgt ggtggaattc gccaagcgga tcgacggctt catggaactg
     3541 tgccagaacg atcagatcgt gctgctgaag gccggcagcc tggaagtggt gttcatcaga
     3601 atgtgccggg ccttcgatag ccagaacaac accgtgtact tcgacgggaa gtacgctagc
     3661 cccgacgtgt tcaagtctct gggctgcgag gacttcatca gcttcgtgtt cgagttcggc
     3721 aagagcctgt gcagcatgca cctgacagag gacgagatcg ccctgttctc tgctttcgtg
     3781 ctgatgagcg ccgacagaag ctggctgcaa gagaaagtga agatcgagaa gctgcaacag
     3841 aagattcagc tggccctgca acacgtgctc cagaagaacc acagagagga cggcatcctg
     3901 accaagctga tctgcaaggt gtccacactg agagccctgt gcggcagaca caccgagaaa
     3961 ctgatggcct tcaaggctat ctaccccgac atcgtgcggc tgcacttccc acctctgtac
     4021 aaagagctgt tcaccagcga gttcgagccc gccatgcaga tcgatggatg ataaggatcc
     4081 cccgggctgc aggaattcga gcatcttacc gccatttatt cccatatttg ttctgttttt
     4141 cttgatttgg gtatacattt aaatgttaat aaaacaaaat ggtggggcaa tcatttacat
     4201 ttttagggat atgtaattac tagttcaggt gtattgccac aagacaaaca tgttaagaaa
     4261 ctttcccgtt atttacgctc tgttcctgtt aatcaacctc tggattacaa aatttgtgaa
     4321 agattgactg atattcttaa ctatgttgct ccttttacgc tgtgtggata tgctgcttta
     4381 atgcctctgt atcatgctat tgcttcccgt acggctttcg ttttctcctc cttgtataaa
     4441 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tccgtcaacg tggcgtggtg
     4501 tgctctgtgt ttgctgacgc aacccccact ggctggggca ttgccaccac ctgtcaactc
     4561 ctttctggga ctttcgcttt ccccctcccg atcgccacgg cagaactcat cgccgcctgc
     4621 cttgcccgct gctggacagg ggctaggttg ctgggcactg ataattccgt ggtgttgtcg
     4681 gggaagctga cgtcctttgc aattcgatat caagctgtac cttctagatg tacattagag
     4741 tgagcgcttc tagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     4801 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     4861 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     4921 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     4981 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     5041 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     5101 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5161 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5221 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     5281 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5341 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     5401 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     5461 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5521 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5581 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     5641 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5701 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5761 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5821 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5881 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5941 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6001 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     6061 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6121 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6181 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6241 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6301 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6361 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6421 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6481 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6541 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6601 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6661 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6721 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6781 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6841 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6901 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6961 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7021 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7081 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7141 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7201 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7261 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7321 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7381 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7441 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7501 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7561 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7621 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7681 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7741 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7801 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7861 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7921 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7981 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     8041 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     8101 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     8161 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     8221 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     8281 agctgca
//
