LOCUS       sequence-450415-        8077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor RBPJ with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8077)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 8077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-RBPJ-Brd 204
FEATURES             Location/Qualifiers
     source          1..8077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1809..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1960..2366
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2395..2404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4081..4484
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             4603..4836
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4908..5029
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4945..4964)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4999..5018
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5069..5204
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5224..5243)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5225..5243)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5253..5270)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5253..5269)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5262..5284)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5411..5866
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5498..5517)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5708..5729
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5892..5996
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5997..6065,6066..6857)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6215..6234)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7028..7616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7517..7536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7770..7787
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7904..7925
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7940..7945,7946..7963,7964..7970)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7978..7994
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7983..8005
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8002..8018
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8002..8018
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8037..8057
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8039..8057
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1921 aattttatcg atcacgagac tagcctcgag ctggctgcag taacgccatt ttgcaaggca
     1981 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2041 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2101 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2161 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2221 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2281 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2341 gtcctccgac agactgagtc ggccggtcga atcaagctta tcgataccgt cgacgccacc
     2401 atggcctgga tcaagcggaa gtttggcgag cggcctcctc caaagagact gaccagagaa
     2461 gccatgcgga actacctgaa agagcggggc gatcagaccg tgctgattct gcatgccaag
     2521 gtggcccaga agtcctacgg caacgagaag cggttcttct gccctcctcc ttgcgtgtac
     2581 ctgatgggct ctggctggaa gaaaaagaaa gaacagatgg aacgcgacgg ctgctccgag
     2641 caagagtctc agccttgtgc cttcatcggc atcggcaaca gcgaccaaga gatgcagcag
     2701 ctgaacctgg aaggcaagaa ctactgcacc gccaagacac tgtacatcag cgactccgac
     2761 aagcgcaagc acttcatgct gagcgtgaag atgttctacg gcaatagcga cgatatcggc
     2821 gtgttcctga gcaagcggat caaagtgatc agcaagccca gcaagaagaa gcagagcctg
     2881 aagaacgccg acctgtgtat cgccagcgga acaaaagtgg ccctgttcaa ccggctgaga
     2941 agccagaccg tgtccaccag atacctgcac gtggaaggcg gcaatttcca cgccagttct
     3001 cagcagtggg gcgccttctt catccatctg ctggacgacg atgagagcga gggcgaagag
     3061 ttcaccgtca gagatggcta catccactac ggccagacag tgaagctcgt gtgtagcgtg
     3121 acaggcatgg ccctgcctcg gctgatcatc agaaaggtgg acaagcagac agccctgctg
     3181 gatgccgacg atcctgtgtc tcagctgcac aagtgcgcct tctacctgaa ggacaccgag
     3241 cggatgtacc tgtgcctgag ccaagagcgg atcatccagt tccaagccac accttgtcct
     3301 aaagaaccca acaaagagat gatcaacgac ggcgccagct ggaccatcat cagcacagat
     3361 aaggccgagt acaccttcta cgaaggcatg ggcccagtgc tggctcctgt gacacctgtg
     3421 ccagtggtgg aaagcctcca gcttaatggc ggcggagatg tggctatgct ggaactgacc
     3481 ggccagaact tcacccctaa cctgagagtt tggttcggcg acgtggaagc cgagacaatg
     3541 tacagatgcg gcgagagcat gctgtgcgtg gtgcctgata tctccgcctt cagagaaggc
     3601 tggcgctggg tccgacagcc tgttcaggtt ccagtgacac tcgtgcggaa cgacggcatc
     3661 atctacagca ccagcctgac cttcacctac acacccgagc ctggacctag acctcactgt
     3721 tctgctgccg gcgctatcct gagagccaac tcttctcagg tgccacctaa cgagagcaac
     3781 accaacagcg agggcagcta caccaacgcc agcaccaata gcacaagcgt gaccagcagc
     3841 accgccaccg tggtgtctta ataaggatcc cccgggctgc aggaattcga gcatcttacc
     3901 gccatttatt cccatatttg ttctgttttt cttgatttgg gtatacattt aaatgttaat
     3961 aaaacaaaat ggtggggcaa tcatttacat ttttagggat atgtaattac tagttcaggt
     4021 gtattgccac aagacaaaca tgttaagaaa ctttcccgtt atttacgctc tgttcctgtt
     4081 aatcaacctc tggattacaa aatttgtgaa agattgactg atattcttaa ctatgttgct
     4141 ccttttacgc tgtgtggata tgctgcttta atgcctctgt atcatgctat tgcttcccgt
     4201 acggctttcg ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4261 tggcccgttg tccgtcaacg tggcgtggtg tgctctgtgt ttgctgacgc aacccccact
     4321 ggctggggca ttgccaccac ctgtcaactc ctttctggga ctttcgcttt ccccctcccg
     4381 atcgccacgg cagaactcat cgccgcctgc cttgcccgct gctggacagg ggctaggttg
     4441 ctgggcactg ataattccgt ggtgttgtcg gggaagctga cgtcctttgc aattcgatat
     4501 caagctgtac cttctagatg tacattggcc gaagcgcttc tagaccaatg acttacaagg
     4561 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     4621 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     4681 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     4741 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     4801 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     4861 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     4921 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4981 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     5041 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5101 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     5161 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     5221 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     5281 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     5341 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     5401 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5461 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5521 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5581 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5641 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5701 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5761 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5821 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     5881 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5941 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6001 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6061 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6121 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6181 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6241 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6301 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6361 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6421 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6481 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6541 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6601 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6661 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6721 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6781 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6841 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6901 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6961 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7021 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7081 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7141 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7201 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7261 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7321 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7381 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7441 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7501 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7561 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7621 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7681 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7741 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7801 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     7861 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     7921 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     7981 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     8041 ttaaccctca ctaaagggaa caaaagctgg agctgca
//
