LOCUS       sequence-450418-        8728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor NFATC1 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8728)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 8728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-NFATC1-Brd 201
FEATURES             Location/Qualifiers
     source          1..8728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1809..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1960..2366
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    4732..5135
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             5254..5487
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5559..5680
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5596..5615)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5650..5669
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5720..5855
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5875..5894)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5876..5894)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5904..5921)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5904..5920)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5913..5935)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6062..6517
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6149..6168)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6359..6380
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6543..6647
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6648..6716,6717..7508)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6866..6885)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7679..8267
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8168..8187
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8421..8438
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8555..8576
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8591..8596,8597..8614,8615..8621)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8629..8645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8634..8656
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8653..8669
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8653..8669
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8688..8708
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8690..8708
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1921 aattttatcg atcacgagac tagcctcgag ctggctgcag taacgccatt ttgcaaggca
     1981 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2041 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2101 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2161 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2221 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2281 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2341 gtcctccgac agactgagtc ggccggtcga atcaagctta tcgataccgt cgacgccacc
     2401 atgaccggcc tggaagatca agagttcgac ttcgagttcc tgttcgagtt caaccagaga
     2461 gatgaaggcg ccgctgccgc cgctcctgag cattatggat acgccagcag caacgtgtcc
     2521 cctgctttgc ctctgcctac cgctcactct acactgcctg ctccatgcca caacctccag
     2581 acaagcaccc ctggcatcat ccctccagcc gatcatcctt ctggatacgg cgctgctctt
     2641 gatggcggac ctgccggcta ttttctgagc agcggacaca ccagacctga tggcgctcct
     2701 gctctggaaa gccccagaat cgagatcacc agctgcctgg gcctgtacca caacaacaac
     2761 cagttcttcc acgacgtcga ggtggaagat gtgctgccca gctccaagag aagccctagc
     2821 acagccacac tgagcctgcc tagcctggaa gcctacaggg accctagctg tctgtctcct
     2881 gccagcagcc tgagcagcag aagctgtaat agcgaggcca gcagctacga gagcaactac
     2941 agctacccct acgcctctcc acagactagc ccttggcaga gcccttgcgt gtccccaaag
     3001 accaccgatc ctgaggaagg cttccctaga ggcctgggag cttgtacact gctgggctct
     3061 ccaagacaca gcccctccac atctccaaga gccagcgtga cagaggaaag ctggctgggc
     3121 gccagaagct ctagaccagc cagtccttgc aacaagcgga agtacagcct gaacggccgg
     3181 cagcctcctt acagccctca ccattctcca acaccttctc cacacggcag ccccagagtg
     3241 tccgtgaccg atgattcttg gctgggcaac accacacagt acaccagctc tgccatcgtg
     3301 gccgccatta acgccctgac cacagatagc agcctggatc tcggagatgg cgtgcccgtg
     3361 aagtccagaa agaccacact ggaacagcct ccaagcgtgg ccctgaaggt ggaacctgtg
     3421 ggagaagatc tgggcagccc tcctcctcct gccgattttg cccctgagga ctacagcagc
     3481 ttccagcata tccggaaagg cggcttctgc gaccagtatc tggctgtgcc tcagcaccct
     3541 taccagtggg ccaagcctaa gcctctgagc cccacaagct acatgagccc aacactgccc
     3601 gctctggatt ggcagctccc ttctcactct ggcccctacg agctgagaat cgaggtgcag
     3661 cctaagagcc accacagagc ccactacgag acagagggat ctagaggcgc cgtgaaagcc
     3721 tctgctggcg gacatcctat cgttcagctg cacggctacc tggaaaacga gcccctgatg
     3781 ctccagctgt ttatcggcac cgccgacgac agactgctga ggccacatgc cttttaccag
     3841 gtgcaccgga tcaccggcaa gaccgtgtct acaacaagcc acgaggccat cctgagcaac
     3901 accaaggtgc tggaaatccc tctgctgccc gagaacagca tgagagccgt gatcgattgt
     3961 gccggcatcc tgaagctgcg gaacagcgat atcgaactgc ggaagggcga aaccgacatc
     4021 ggccggaaga acacaagagt gcggctggtg ttcagagtgc acgtgccaca gccaagcggc
     4081 agaaccctgt ctctgcaagt ggccagcaat cccatcgagt gtagccagag aagcgcccaa
     4141 gagctgcccc tggtggaaaa gcagagcacc gatagctacc ctgtcgttgg cggcaagaaa
     4201 atggtgctga gcggccacaa cttcctccag gacagcaaag tgatcttcgt ggaaaaggcc
     4261 ccagacggcc accacgtgtg ggagatggaa gccaagaccg acagggacct gtgcaagccc
     4321 aatagcctgg tggtggaaat tcctccattc cggaaccagc ggatcacaag ccctgtgcac
     4381 gtgtccttct acgtgtgcaa cggcaagaga aagcggagcc agtaccagcg gttcacctac
     4441 ctgccagcca acggcaatgc catcttcctg accgtgtcca gagaacacga gcgcgtgggc
     4501 tgcttcttct gataaggatc ccccgggctg caggaattcg agcatcttac cgccatttat
     4561 tcccatattt gttctgtttt tcttgatttg ggtatacatt taaatgttaa taaaacaaaa
     4621 tggtggggca atcatttaca tttttaggga tatgtaatta ctagttcagg tgtattgcca
     4681 caagacaaac atgttaagaa actttcccgt tatttacgct ctgttcctgt taatcaacct
     4741 ctggattaca aaatttgtga aagattgact gatattctta actatgttgc tccttttacg
     4801 ctgtgtggat atgctgcttt aatgcctctg tatcatgcta ttgcttcccg tacggctttc
     4861 gttttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4921 gtccgtcaac gtggcgtggt gtgctctgtg tttgctgacg caacccccac tggctggggc
     4981 attgccacca cctgtcaact cctttctggg actttcgctt tccccctccc gatcgccacg
     5041 gcagaactca tcgccgcctg ccttgcccgc tgctggacag gggctaggtt gctgggcact
     5101 gataattccg tggtgttgtc ggggaagctg acgtcctttg caattcgata tcaagctgta
     5161 ccttctagat gtacaacata tgaagcgctt ctagaccaat gacttacaag gcagctgtag
     5221 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     5281 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     5341 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     5401 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     5461 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     5521 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     5581 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5641 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     5701 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5761 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     5821 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     5881 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     5941 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     6001 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     6061 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6121 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6181 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6241 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     6301 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6361 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6421 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6481 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     6541 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6601 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6661 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6721 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6781 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6841 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6901 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6961 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7021 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7081 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7141 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     7201 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7261 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7321 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7381 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7441 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7501 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7561 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7621 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7681 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7741 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7801 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7861 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7921 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7981 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8041 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8101 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8161 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8221 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8281 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8341 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8401 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     8461 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     8521 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     8581 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     8641 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     8701 actaaaggga acaaaagctg gagctgca
//
