LOCUS       sequence-450419-        8434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor MYB with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8434)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 8434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-MYB-Brd 199
FEATURES             Location/Qualifiers
     source          1..8434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1809..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1960..2366
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2395..2404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4438..4841
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             4960..5193
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5265..5386
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5302..5321)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5356..5375
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5426..5561
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5581..5600)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5582..5600)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5610..5627)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5610..5626)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5619..5641)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5768..6223
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5855..5874)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6065..6086
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6249..6353
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6354..6422,6423..7214)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6572..6591)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7385..7973
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7874..7893
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8127..8144
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8261..8282
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8297..8302,8303..8320,8321..8327)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8335..8351
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8340..8362
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8359..8375
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8359..8375
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8394..8414
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8396..8414
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1921 aattttatcg atcacgagac tagcctcgag ctggctgcag taacgccatt ttgcaaggca
     1981 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2041 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2101 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2161 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2221 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2281 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2341 gtcctccgac agactgagtc ggccggtcga atcaagctta tcgataccgt cgacgccacc
     2401 atggccagaa ggcccagaca cagcatctac agcagcgacg aggacgatga ggacttcgag
     2461 atgtgcgacc acgactacga tggcctgctg cctaagagcg gcaagagaca cctgggcaag
     2521 acccggtgga ccagagaaga ggacgagaag ctgaagaaac tggtggaaca gaacggcacc
     2581 gacgactgga aagtgatcgc caactacctg cctaaccgga ccgatgtgca gtgccagcac
     2641 agatggcaga aggtgctgaa ccccgagctg atcaaaggcc cctggacaaa agaagaggat
     2701 cagcgggtca tcgagctggt gcagaagtac ggacctaagc gttggagcgt gatcgctaag
     2761 cacctgaagg gcagaatcgg caagcagtgc agagagcggt ggcacaacca cctgaatcct
     2821 gaagtgaaga aaaccagctg gactgaggaa gaggaccgga tcatctatca ggcccacaag
     2881 cggctgggca acagatgggc cgaaattgcc aaactgctgc ccggcagaac cgacaacgcc
     2941 atcaagaacc actggaacag caccatgcgg cggaaggtgg aacaagaggg ctacctgcaa
     3001 gagagcagca aggcctctca gcctgccgtg gccaccagct tccagaaaaa ctctcacctg
     3061 atgggcttcg cccaggctcc tcctacagct caattgccag caaccggcca gcctaccgtg
     3121 aacaacgact acagctacta ccacatcagc gaggcccaga acgtgtccag ccacgtgcca
     3181 tatcctgtgg ctctgcacgt gaacatcgtg aacgtgccac agccagccgc cgctgctatt
     3241 cagacccaga atcacacctg tagctacccc ggctggcact ccaccacaat cgccgatcac
     3301 acaagacccc acggcgatag tgcccctgtg tcttgtctgg gagagcacca cagcacacct
     3361 agcctgcctg ctgatcctgg ctctctgcct gaggaatctg cctctcctgc cagatgcatg
     3421 atcgtgcacc agggcaccat cctggacaac gtgaagaacc tgctggaatt cgccgagaca
     3481 ctccagttca tcgacagctt tctgaacacc agcagcaacc acgagaacag cgacctggaa
     3541 atgcccagcc tgacaagcac acctctgatc ggccacaagc tgaccgtgac cacacctttc
     3601 cacagggacc agaccgtgaa aacccagaaa gaaaacaccg tgttcaggac ccctgccatc
     3661 aagcggagca tcctggaaag cagccccaga acacccacac cattcaagca cgccctggcc
     3721 gctcaagaga ttaagtacgg ccctctgaag atgctgcccc agacaccttc tcacctggtg
     3781 gaagatctcc aggacgtgat caagcaagag tccgacgagt ctggcatcgt ggccgagttc
     3841 caagagaatg gccctccact gctgaaaaag attaagcaag aggtggaaag ccccaccgac
     3901 aagtccggca acttcttctg tagccaccac tgggagggcg acagcctgaa tacccagctg
     3961 ttcacccaga ctagccccgt ggccgacgct cctaatatcc tgacatccag cgtgctgatg
     4021 gcccctgcct ctgaggacga ggataatgtg ctgaaggcct ttaccgtgcc taagaacaga
     4081 agcctggcct ctccactcca gccttgtagc tctacatggg agcctgccag ctgcggcaag
     4141 atggaagaac agatgaccag cagctctcag gcccggaaat acgtgaacgc cttcagcgcc
     4201 aggacactgg tcatgtgata aggatccccc gggctgcagg aattcgagca tcttaccgcc
     4261 atttattccc atatttgttc tgtttttctt gatttgggta tacatttaaa tgttaataaa
     4321 acaaaatggt ggggcaatca tttacatttt tagggatatg taattactag ttcaggtgta
     4381 ttgccacaag acaaacatgt taagaaactt tcccgttatt tacgctctgt tcctgttaat
     4441 caacctctgg attacaaaat ttgtgaaaga ttgactgata ttcttaacta tgttgctcct
     4501 tttacgctgt gtggatatgc tgctttaatg cctctgtatc atgctattgc ttcccgtacg
     4561 gctttcgttt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     4621 cccgttgtcc gtcaacgtgg cgtggtgtgc tctgtgtttg ctgacgcaac ccccactggc
     4681 tggggcattg ccaccacctg tcaactcctt tctgggactt tcgctttccc cctcccgatc
     4741 gccacggcag aactcatcgc cgcctgcctt gcccgctgct ggacaggggc taggttgctg
     4801 ggcactgata attccgtggt gttgtcgggg aagctgacgt cctttgcaat tcgatatcaa
     4861 gctgtacctt ctagatgtac aagcttacaa gcgcttctag accaatgact tacaaggcag
     4921 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     4981 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     5041 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5101 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5161 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     5221 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     5281 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5341 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     5401 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5461 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     5521 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     5581 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     5641 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     5701 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     5761 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5821 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5881 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5941 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6001 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6061 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6121 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6181 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     6241 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6301 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6361 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6421 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6481 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6541 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     6601 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6661 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6721 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6781 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6841 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     6901 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6961 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7021 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7081 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7141 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7201 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7261 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7321 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7381 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7441 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7501 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7561 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7621 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7681 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7741 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7801 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7861 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7921 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7981 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8041 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8101 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8161 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     8221 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     8281 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     8341 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     8401 accctcacta aagggaacaa aagctggagc tgca
//
