LOCUS       sequence-450420-        8030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor MEF2C with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8030)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-MEF2C-Brd 98
FEATURES             Location/Qualifiers
     source          1..8030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..132
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             179..359
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(360..586)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(614..634)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(614..632)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(653..669)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(653..669)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(666..688)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    677..693
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(701..707,708..725,726..731))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    746..767
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(884..901)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1055..1643)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1135..1154)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1814..2605,2606..2674))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2437..2456
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2675..2779)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2805..3260)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2942..2963)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3154..3173
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3387..3409
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3401..3418
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3402..3418
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3428..3447
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3428..3446
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3467..3602
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3642..3763
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3653..3672)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3707..3726
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3835..4068
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4187..4590
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     promoter        complement(6258..6664)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    6698..6815
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7082..7126)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7311..7544
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca aaatttttgg
       61 cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct
      121 gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg ggcgccactg
      181 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
      241 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
      301 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagacccg
      361 tttatgtatc gagctaggca cttaaataca atatctctgc aatgcggcaa ttcagtggtt
      421 cgtccaatcc atgtcagacc cgtctgttgc cttcctaata aggcacgatc gtaccacctt
      481 acttccacca atcggcatgc acggtgcttt ttctctcctt gtaaggcatg ttgctaactc
      541 atcgttacca tgttgcaaga ctacaagagt attgcataag actacattaa gcttgcagct
      601 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
      661 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
      721 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
      781 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      841 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
      901 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      961 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1021 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1081 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1141 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1201 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1261 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1321 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1381 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1441 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1501 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1561 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1621 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1681 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1741 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1801 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1861 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     1921 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1981 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     2041 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     2101 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     2161 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     2221 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     2281 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     2341 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     2401 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     2461 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     2521 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     2581 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     2641 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     2701 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     2761 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa
     2821 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     2881 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     2941 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     3001 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     3061 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     3121 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     3181 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     3241 tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3301 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3361 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3421 gcgcgcgtaa tacgactcac tatagggcga attgggtacg tccctaggcc tccaaaaaag
     3481 cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata
     3541 aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg
     3601 atgggcggag ttaggggcgg gatagctaga gccagacatg ataagataca ttgatgagtt
     3661 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     3721 tattgcttta tttgtaacca ttataagctg caataaacaa gttcctctca ctctctgata
     3781 ttcatttctt tgcaagttat aaatactgaa taataagatg acatgaacta ctactgctag
     3841 agattttcca cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca
     3901 gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt
     3961 tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac
     4021 aagcaaaaag cagatcttgt cttcgttggg agtgaattag cccttccagt cccccctttt
     4081 cttttaaaaa gtggctaaga tctacagctg ccttgtaagt cattggtcta gaagcgctgc
     4141 caataatgta catctagaag gtacagcttg atatcgaatt gcaaaggacg tcagcttccc
     4201 cgacaacacc acggaattat cagtgcccag caacctagcc cctgtccagc agcgggcaag
     4261 gcaggcggcg atgagttctg ccgtggcgat cgggaggggg aaagcgaaag tcccagaaag
     4321 gagttgacag gtggtggcaa tgccccagcc agtgggggtt gcgtcagcaa acacagagca
     4381 caccacgcca cgttgacgga caacgggcca caactcctca taaagagaca gcaaccagga
     4441 tttatacaag gaggagaaaa cgaaagccgt acgggaagca atagcatgat acagaggcat
     4501 taaagcagca tatccacaca gcgtaaaagg agcaacatag ttaagaatat cagtcaatct
     4561 ttcacaaatt ttgtaatcca gaggttgatt aacaggaaca gagcgtaaat aacgggaaag
     4621 tttcttaaca tgtttgtctt gtggcaatac acctgaacta gtaattacat atccctaaaa
     4681 atgtaaatga ttgccccacc attttgtttt attaacattt aaatgtatac ccaaatcaag
     4741 aaaaacagaa caaatatggg aataaatggc ggtaagatgc tcgaattcct gcagcccggg
     4801 ggatccgacc ggtctatgtt gcccatcctt cagaaagtcg catgcgcttg actgagggac
     4861 tttccctttc gtccggcgaa ggtctggtga gtccaatggg ggagtggaat tcgttccggt
     4921 gatcctctcg gtcgctcccg tcgtacgaac tgctacagct gctcaagctg tcaacaggag
     4981 atctccccgc ctcgtggcgc gtgtgttgtg ggtatctcga aggggtggtg gtacggtctc
     5041 taggaggaga aacaggttct gacttgatgt tgaggctttg agtagaaggc agggagagat
     5101 ttgaactctg agataaatga gtgctagtgc aagctcccaa ctgactgagg gcagatggtg
     5161 gcatgttatg taggtgttgc tgttgccagc cagttactga accaaggtga agagcgctgg
     5221 cggtgttaaa cccagacaga gatgacaggt ctgcactact cagagagtac tcggtaccat
     5281 atgttgttga aatggctgat ggatatcctc ccattccttg tcctggtaaa gtaggagttg
     5341 ctacggaaac cactggggta gccaatgact gagccgactg ggagttattt atcctttgat
     5401 tcactgatgg catcgtattc ttgctgcctg gtggaataag aactcggaga tctggtttac
     5461 ggttattcat tcctaaattc attgggggag gagattttgc ttgcatattc ttgttcaagt
     5521 taccaggtga gaccagcaga cctggtgagt ttcggggatt gccatacccg ttccctgcac
     5581 tggtgcctgc accagacgtg aggtctccac ccatcagacc acctgtgtta cctgcacttg
     5641 gaggtcgatg tgttacacca ggagacatac tattcctctg cagagaaggg tgagccagtg
     5701 gcaataggtt ggggtttccc agtgagctga cagggttgct gtacaccaaa ctgttgtggc
     5761 tggacactgg gatggagact ggcatctcga agttgggagg tggaacagca ggaattttat
     5821 gactcttgat catattatca aattcttcat taattttttt gtatttttct tcagtgcgtg
     5881 gggtgagtgc ataagaggag tcgggatcgg ggctttcaca gcctttgttt tctttcttgt
     5941 tcaatgcctc cacgatgtct gagtttgtcc ggctctcatg cggctcgttg tactccgtgt
     6001 acttgagaag cactttgtcc atgtcggtgc tggcatactg gaacagcttg ttggtgctgt
     6061 tgaagatgat cagcgcaatc tcacagtcac acagcacgct cagctcataa gccttcttca
     6121 tcaacccaaa tttcctcttt gtaaatgtca cctgtctgtt acgttcatcc ataatcctcg
     6181 taatctgaat cttttttctc cccatttcga accgcgggct agcggatccg tcgacggtat
     6241 cgataagctt gattcgaccg gccgactcag tctgtcggag gactggcgcg ccgagtgagg
     6301 ggttgtgagc tcttttatag agctcgggaa gcagaagcgc gcgaacagaa gcgagaagca
     6361 ggctgattgg ttaattcaaa taaggcgcag ggtcatttca ggtccttggg ggagcctgga
     6421 aacatctgat gggtcttaag aaactgctga gggttgggcc atatctgggg accatctgtt
     6481 cttggcctcg ggccggggcc gaaactgcgg tgaccatctg ttcttggccc cgggccgggg
     6541 ccgaaactgc tcaccgcaga tatcctgttt ggcccaacgt tagctatttt catgtacccg
     6601 cccttgatct gaacttctct attcttggtt tggtattttt ccatgccttg caaaatggcg
     6661 ttactgcagc cagctcgagg ctagtctcgt gatcgataaa attttgaatt tttgtaattt
     6721 gtttttgtaa ttctttagtt tgtatgtctg ttgctattat gtctactatt ctttcccctg
     6781 cactgtaccc cccaatcccc ccttttcttt taaaagttaa ccgataccgt cgagatccgt
     6841 tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg
     6901 gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct
     6961 gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc
     7021 ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc
     7081 acaaacttgc ccatttatct aattccaata attcttgttc attcttttct tgctggtttt
     7141 gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact
     7201 ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc
     7261 aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt
     7321 gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga
     7381 ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct
     7441 gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca
     7501 tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt
     7561 tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt
     7621 ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag
     7681 gtctgaagat cagcggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct
     7741 atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg
     7801 gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt
     7861 agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagg attaactgcg
     7921 aatcgttcta gctccctgct tgcccatact atatgtttta atttatattt tttctttccc
     7981 cctggcctta accgaatttt ttcccatcgc gatctaattc tcccccgctt
//
