LOCUS       sequence-450428-        7511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor HOXD8 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7511)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 7511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-HOXD8-Brd 105
FEATURES             Location/Qualifiers
     source          1..7511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(101..327)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(355..375)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(355..373)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(394..410)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(394..410)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(407..429)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    418..434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(442..448,449..466,467..472))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    487..508
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(625..642)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(796..1384)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(876..895)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1555..2346,2347..2415))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2178..2197
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2416..2520)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2546..3001)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2683..2704)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2895..2914
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3128..3150
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3142..3159
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3143..3159
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3169..3188
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3169..3187
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3208..3343
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3383..3504
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3394..3413)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3448..3467
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3576..3809
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3928..4331
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     promoter        complement(5480..5886)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    5920..6037
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6304..6348)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6533..6766
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7259..7384
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             join(7431..7511,1..100)
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
       61 agagctccca ggctcagatc tggtctaacc agagagaccc gtttatgtat cgagctaggc
      121 acttaaatac aatatctctg caatgcggca attcagtggt tcgtccaatc catgtcagac
      181 ccgtctgttg ccttcctaat aaggcacgat cgtaccacct tacttccacc aatcggcatg
      241 cacggtgctt tttctctcct tgtaaggcat gttgctaact catcgttacc atgttgcaag
      301 actacaagag tattgcataa gactacatta agcttgcagc tccagctttt gttcccttta
      361 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
      421 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
      481 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
      541 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      601 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      661 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      721 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      781 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      841 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      901 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      961 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1021 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1081 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1141 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1201 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1261 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1321 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1381 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1441 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1501 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     1561 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1621 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1681 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1741 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1801 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1861 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1921 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1981 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     2041 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     2101 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     2161 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     2221 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     2281 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     2341 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     2401 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     2461 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     2521 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
     2581 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     2641 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     2701 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     2761 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     2821 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     2881 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     2941 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     3001 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     3061 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     3121 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca
     3181 ctatagggcg aattgggtac gtccctaggc ctccaaaaaa gcctcctcac tacttctgga
     3241 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat
     3301 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg
     3361 ggatagctag agccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     3421 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     3481 attataagct gcaataaaca agttcctctc actctctgat attcatttct ttgcaagtta
     3541 taaatactga ataataagat gacatgaact actactgcta gagattttcc acactgacta
     3601 aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac acactacttg
     3661 aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag
     3721 ctcccaggct cagatctggt ctaaccagag agacccagta caagcaaaaa gcagatcttg
     3781 tcttcgttgg gagtgaatta gcccttccag tccccccttt tcttttaaaa agtggctaag
     3841 atctacagct gccttgtaag tcattggtct agaagcgctc tgaaggctgt acatctagaa
     3901 ggtacagctt gatatcgaat tgcaaaggac gtcagcttcc ccgacaacac cacggaatta
     3961 tcagtgccca gcaacctagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttct
     4021 gccgtggcga tcgggagggg gaaagcgaaa gtcccagaaa ggagttgaca ggtggtggca
     4081 atgccccagc cagtgggggt tgcgtcagca aacacagagc acaccacgcc acgttgacgg
     4141 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
     4201 acgaaagccg tacgggaagc aatagcatga tacagaggca ttaaagcagc atatccacac
     4261 agcgtaaaag gagcaacata gttaagaata tcagtcaatc tttcacaaat tttgtaatcc
     4321 agaggttgat taacaggaac agagcgtaaa taacgggaaa gtttcttaac atgtttgtct
     4381 tgtggcaata cacctgaact agtaattaca tatccctaaa aatgtaaatg attgccccac
     4441 cattttgttt tattaacatt taaatgtata cccaaatcaa gaaaaacaga acaaatatgg
     4501 gaataaatgg cggtaagatg ctcgaattcc tgcagcccgg gggatccgac cggtctaatt
     4561 tgtcaggcct tcggctctgt cttcctccag ctcttgggct tcctttttcg tttccccgtc
     4621 cttcacctcc tgccgggaaa cgggaaattt gtccttgttg ttttcctttt tccatttcat
     4681 tctcctgttc tggaaccaga tttttacctg tctctcggtg agggctaggg cgtgggaaac
     4741 ctcgattctt cttttcctgg tcagataggg gttaaaaaga aattcctttt ccaactctag
     4801 agtttggaag cgactgtagg tttgtcttcc tcttcgtcta ccaggagctg cttgtggtct
     4861 catccacgga aacatttgag aaggagacga gctctgattt aagtggtctg ggtcctcgcc
     4921 aatattacca ctggacgatt tacagtcagg atattgtacc agctcggcct cttgctgggt
     4981 cgtaaaaatc ggctgtctct gtaagttatc gtatccgtaa aacttcgcgg gctccccgtg
     5041 acaggcaatc ccgccgcagg ggggaggtgg cggcggaggc ggaggatgcg gaggaggagg
     5101 gggggcggcc tggtaggcag cggccgggct gcccccgccg gggtggaagt actcctggcc
     5161 ccggccttcc ctaccgccgc acccagtccc ggagggcggc ggggacgggt gcgggtgcgc
     5221 gtgcgcctgc gggggcgcgt gcgggaagcc ggcggcgctg ttgccataga gctgcagggc
     5281 tgctgcggcg gcggcgtgac ggccgccgac ctcgggcgcg aagtgacagt cgtagtaagt
     5341 gggattgatg gcctcgcccg ccgccgccgc cgccgcagcc gccgccttgt acttggagta
     5401 cagcgggttc acgaagtacg aactcatttc gaaccgcggg ctagcggatc cgtcgacggt
     5461 atcgataagc ttgattcgac cggccgactc agtctgtcgg aggactggcg cgccgagtga
     5521 ggggttgtga gctcttttat agagctcggg aagcagaagc gcgcgaacag aagcgagaag
     5581 caggctgatt ggttaattca aataaggcgc agggtcattt caggtccttg ggggagcctg
     5641 gaaacatctg atgggtctta agaaactgct gagggttggg ccatatctgg ggaccatctg
     5701 ttcttggcct cgggccgggg ccgaaactgc ggtgaccatc tgttcttggc cccgggccgg
     5761 ggccgaaact gctcaccgca gatatcctgt ttggcccaac gttagctatt ttcatgtacc
     5821 cgcccttgat ctgaacttct ctattcttgg tttggtattt ttccatgcct tgcaaaatgg
     5881 cgttactgca gccagctcga ggctagtctc gtgatcgata aaattttgaa tttttgtaat
     5941 ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc
     6001 tgcactgtac cccccaatcc ccccttttct tttaaaagtt aaccgatacc gtcgagatcc
     6061 gttcactaat cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc
     6121 gggcctgtcg ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc
     6181 ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc
     6241 tcctactatc attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt
     6301 ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt
     6361 ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca
     6421 ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt
     6481 ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg
     6541 ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca
     6601 gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg
     6661 ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc
     6721 catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct
     6781 tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat
     6841 ttttactact ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc
     6901 aggtctgaag atcagcggcc gcttgctgtg cggtggtctt acttttgttt tgctcttcct
     6961 ctatcttgtc taaagcttcc ttggtgtctt ttatctctat cctttgatgc acacaataga
     7021 gggttgctac tgtattatat aatgatctaa gttcttctga tcctgtctga agggatggtt
     7081 gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc taacaggcca ggattaactg
     7141 cgaatcgttc tagctccctg cttgcccata ctatatgttt taatttatat tttttctttc
     7201 cccctggcct taaccgaatt ttttcccatc gcgatctaat tctcccccgc ttaatactga
     7261 cgctctcgca cccatctctc tccttctagc ctccgctagt caaaattttt ggcgtactca
     7321 ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag
     7381 agagctcctc tggtttccct ttcgctttca agtccctgtt cgggcgccac tgctagagat
     7441 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
     7501 ggcacacact a
//
