LOCUS       sequence-450429-        8119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor IKZF2 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8119)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 8119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-IKZF2-Brd 194
FEATURES             Location/Qualifiers
     source          1..8119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1809..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1960..2366
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2395..2404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4123..4526
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             4645..4878
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4950..5071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4987..5006)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5041..5060
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5111..5246
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5266..5285)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5267..5285)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5295..5312)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5295..5311)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5304..5326)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5453..5908
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5540..5559)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5750..5771
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5934..6038
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6039..6107,6108..6899)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6257..6276)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7070..7658
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7559..7578
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7812..7829
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7946..7967
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7982..7987,7988..8005,8006..8012)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8020..8036
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8025..8047
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8044..8060
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8044..8060
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8079..8099
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8081..8099
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1921 aattttatcg atcacgagac tagcctcgag ctggctgcag taacgccatt ttgcaaggca
     1981 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2041 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2101 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2161 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2221 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2281 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2341 gtcctccgac agactgagtc ggccggtcga atcaagctta tcgataccgt cgacgccacc
     2401 atggaaaccg aggccatcga cggctacatc acctgtgaca acgagctgag ccccgagcgc
     2461 gagcactcta acatggccat cgatctgacc agcagcaccc ctaatggcca gcacgcctct
     2521 ccaagccaca tgaccagcac caacagcgtg aagctggaaa tgcagagcga cgaggaatgc
     2581 gacagaaagc ccctgagcag agaggacgag atcagaggcc acgatgaggg cagcagcctg
     2641 gaagaacctc tgatcgagtc tagcgaggtg gccgacaacc ggaaggtgca agaacttcaa
     2701 ggcgaaggcg gcatcagact gcccaacggc gaaagaccct tccactgcaa tcagtgcggc
     2761 gccagcttta cccagaaggg caatctgctg cggcacatca agctgcactc tggcgagaag
     2821 cccttcaagt gccccttctg tagctacgcc tgtcggagaa gagatgccct gacaggccac
     2881 ctgagaacac actctgtggg caagccccac aagtgcaact actgcggcag aagctacaag
     2941 cagcggagca gcctcgagga acacaaagag cggtgccaca actacctcca gaatgtgtct
     3001 atggaagccg ccggacaagt gatgagccac cacgttccac ctatggaaga ttgcaaagaa
     3061 caagagccca tcatggacaa caacatcagc ctggtgcctt tcgagcggcc tgccgtgatt
     3121 gagaagctga ccggcaacat gggcaagaga aagagcagca caccccagaa attcgtgggc
     3181 gagaaactga tgcggttcag ctaccccgac atccacttcg acatgaacct gacctacgag
     3241 aaagaggccg agctgatgca gtcccacatg atggaccagg ccatcaacaa cgccatcacc
     3301 tacctgggag ccgaggctct gcatcccctg atgcaacacc ctcctagcac aatcgccgaa
     3361 gtggcccctg tgatcagcag cgcttacagc caggtgtacc atcctaaccg gatcgagagg
     3421 cccatcagca gagagacagc cgacagccac gagaacaaca tggacggccc tatcagcctg
     3481 atcagaccca agagcagacc ccaagagaga gaggctagcc ccagcaacag ctgcctggat
     3541 agcacagata gcgagagcag ccacgacgac caccagagct atcagggaca ccccgctctg
     3601 aaccctaaga gaaagcagag ccccgcctac atgaaggaag atgtgaaggc cctggacacc
     3661 acaaaggccc ctaagggcag cctgaaggac atctacaagg tgttcaacgg cgagggcgag
     3721 cagatccggg cctttaagtg cgagcactgc cgggtgctgt ttctggacca cgtgatgtac
     3781 accatccaca tgggctgcca cggctacagg gaccctctgg aatgcaacat ctgcggctac
     3841 cgcagccagg acagatacga gttcagcagc cacatcgtgc ggggcgagca cacctttcat
     3901 tgataaggat cccccgggct gcaggaattc gagcatctta ccgccattta ttcccatatt
     3961 tgttctgttt ttcttgattt gggtatacat ttaaatgtta ataaaacaaa atggtggggc
     4021 aatcatttac atttttaggg atatgtaatt actagttcag gtgtattgcc acaagacaaa
     4081 catgttaaga aactttcccg ttatttacgc tctgttcctg ttaatcaacc tctggattac
     4141 aaaatttgtg aaagattgac tgatattctt aactatgttg ctccttttac gctgtgtgga
     4201 tatgctgctt taatgcctct gtatcatgct attgcttccc gtacggcttt cgttttctcc
     4261 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtccgtcaa
     4321 cgtggcgtgg tgtgctctgt gtttgctgac gcaaccccca ctggctgggg cattgccacc
     4381 acctgtcaac tcctttctgg gactttcgct ttccccctcc cgatcgccac ggcagaactc
     4441 atcgccgcct gccttgcccg ctgctggaca ggggctaggt tgctgggcac tgataattcc
     4501 gtggtgttgt cggggaagct gacgtccttt gcaattcgat atcaagctgt accttctaga
     4561 tgtacatgtt gaggagcgct tctagaccaa tgacttacaa ggcagctgta gatcttagcc
     4621 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     4681 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     4741 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     4801 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     4861 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     4921 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     4981 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5041 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     5101 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5161 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     5221 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     5281 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     5341 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     5401 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     5461 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5521 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5581 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5641 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5701 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5761 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5821 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5881 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     5941 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6001 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6061 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6121 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6181 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6241 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6301 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6361 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6421 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6481 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6541 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6601 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6661 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6721 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6781 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6841 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6901 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6961 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7021 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7081 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7141 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7201 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7261 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7321 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7381 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7441 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7501 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7561 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7621 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7681 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7741 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7801 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7861 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     7921 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     7981 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     8041 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     8101 aacaaaagct ggagctgca
//
