LOCUS       sequence-450430-        8975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor NR3C1 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8975)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 8975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-NR3C1-Brd 106
FEATURES             Location/Qualifiers
     source          1..8975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    480..605
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             652..832
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(833..1059)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1087..1107)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1087..1105)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1126..1142)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1126..1142)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1139..1161)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1150..1166
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1174..1180,1181..1198,1199..1204))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1219..1240
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1357..1374)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1528..2116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1608..1627)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2287..3078,3079..3147))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2910..2929
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3148..3252)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3278..3733)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3415..3436)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3627..3646
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3860..3882
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3874..3891
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3875..3891
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3901..3920
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3901..3919
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3940..4075
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4115..4236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4126..4145)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4180..4199
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4308..4541
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4660..5063
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5287..7623)
                     /codon_start=1
                     /gene="Homo sapiens NR3C1"
                     /product="human glucocorticoid receptor, isoform alpha"
                     /label=glucocorticoid receptor
                     /note="acts as a transcription factor that binds to 
                     glucocorticoid response elements, and as a modulator of 
                     other transcription factors"
                     /translation="MDSKESLTPGREENPSSVLAQERGDVMDFYKTLRGGATVKVSASS
                     PSLAVASQSDSKQRRLLVDFPKGSVSNAQQPDLSKAVSLSMGLYMGETETKVMGNDLGF
                     PQQGQISLSSGETDLKLLEESIANLNRSTSVPENPKSSASTAVSAAPTEKEFPKTHSDV
                     SSEQQHLKGQTGTNGGNVKLYTTDQSTFDILQDLEFSSGSPGKETNESPWRSDLLIDEN
                     CLLSPLAGEDDSFLLEGNSNEDCKPLILPDTKPKIKDNGDLVLSSPSNVTLPQVKTEKE
                     DFIELCTPGVIKQEKLGTVYCQASFPGANIIGNKMSAISVHGVSTSGGQMYHYDMNTAS
                     LSQQQDQKPIFNVIPPIPVGSENWNRCQGSGDDNLTSLGTLNFPGRTVFSNGYSSPSMR
                     PDVSSPPSSSSTATTGPPPKLCLVCSDEASGCHYGVLTCGSCKVFFKRAVEGRQHNYLC
                     AGRNDCIIDKIRRKNCPACRYRKCLQAGMNLEARKTKKKIKGIQQATTGVSQETSENPG
                     NKTIVPATLPQLTPTLVSLLEVIEPEVLYAGYDSSVPDSTWRIMTTLNMLGGRQVIAAV
                     KWAKAIPGFRNLHLDDQMTLLQYSWMFLMAFALGWRSYRQSSANLLCFAPDLIINEQRM
                     TLPCMYDQCKHMLYVSSELHRLQVSYEEYLCMKTLLLLSSVPKDGLKSQELFDEIRMTY
                     IKELGKAIVKREGNSSQNWQRFYQLTKLLDSMHEVVENLLNYCFQTFLDKTMSIEFPEM
                     LAEIITNQIPKYSNGNIKKLLFHQK"
     promoter        complement(7676..8082)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    8116..8233
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8500..8544)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8729..8962
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ttttttctct ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa
       61 tttttactac tttatattta tataattcac ttctccaatt gtccctcata tctcctcctc
      121 caggtctgaa gatcagcggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc
      181 tctatcttgt ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag
      241 agggttgcta ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt
      301 tgtagctgtc ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact
      361 gcgaatcgtt ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt
      421 ccccctggcc ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg
      481 acgctctcgc acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc
      541 accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga
      601 gagagctcct ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga
      661 ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac
      721 gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc
      781 ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac ccgtttatgt
      841 atcgagctag gcacttaaat acaatatctc tgcaatgcgg caattcagtg gttcgtccaa
      901 tccatgtcag acccgtctgt tgccttccta ataaggcacg atcgtaccac cttacttcca
      961 ccaatcggca tgcacggtgc tttttctctc cttgtaaggc atgttgctaa ctcatcgtta
     1021 ccatgttgca agactacaag agtattgcat aagactacat taagcttgca gctccagctt
     1081 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     1141 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     1201 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     1261 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     1321 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     1381 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1441 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1501 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1561 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1621 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1681 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1741 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1801 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1861 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1921 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1981 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2041 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2101 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2161 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2221 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2281 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2341 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2401 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     2461 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2521 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2581 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2641 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2701 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2761 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2821 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     2881 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     2941 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3001 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3061 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     3121 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     3181 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3241 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     3301 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     3361 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     3421 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     3481 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     3541 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     3601 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     3661 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     3721 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     3781 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     3841 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
     3901 taatacgact cactataggg cgaattgggt acgtccctag gcctccaaaa aagcctcctc
     3961 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     4021 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     4081 gagttagggg cgggatagct agagccagac atgataagat acattgatga gtttggacaa
     4141 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     4201 ttatttgtaa ccattataag ctgcaataaa caagttcctc tcactctctg atattcattt
     4261 ctttgcaagt tataaatact gaataataag atgacatgaa ctactactgc tagagatttt
     4321 ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc
     4381 acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag
     4441 ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccag tacaagcaaa
     4501 aagcagatct tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa
     4561 aaagtggcta agatctacag ctgccttgta agtcattggt ctagaagcgc tatttagagt
     4621 gtacatctag aaggtacagc ttgatatcga attgcaaagg acgtcagctt ccccgacaac
     4681 accacggaat tatcagtgcc cagcaaccta gcccctgtcc agcagcgggc aaggcaggcg
     4741 gcgatgagtt ctgccgtggc gatcgggagg gggaaagcga aagtcccaga aaggagttga
     4801 caggtggtgg caatgcccca gccagtgggg gttgcgtcag caaacacaga gcacaccacg
     4861 ccacgttgac ggacaacggg ccacaactcc tcataaagag acagcaacca ggatttatac
     4921 aaggaggaga aaacgaaagc cgtacgggaa gcaatagcat gatacagagg cattaaagca
     4981 gcatatccac acagcgtaaa aggagcaaca tagttaagaa tatcagtcaa tctttcacaa
     5041 attttgtaat ccagaggttg attaacagga acagagcgta aataacggga aagtttctta
     5101 acatgtttgt cttgtggcaa tacacctgaa ctagtaatta catatcccta aaaatgtaaa
     5161 tgattgcccc accattttgt tttattaaca tttaaatgta tacccaaatc aagaaaaaca
     5221 gaacaaatat gggaataaat ggcggtaaga tgctcgaatt cctgcagccc gggggatccg
     5281 accggttcac ttttgatgaa acagaagttt tttgatattt ccatttgaat attttggtat
     5341 ctgattggtg atgatttcag ctaacatctc ggggaattca atactcatgg tcttatccaa
     5401 aaatgtttgg aagcaatagt taaggagatt ttcaaccact tcatgcatag aatccaagag
     5461 ttttgtcagt tgataaaacc gctgccagtt ctggctggag tttccttccc tcttgacaat
     5521 ggcttttcct agctctttga tgtaggtcat tctaatttca tcaaatagct cttggctctt
     5581 cagaccgtcc ttaggaactg aagagagaag cagtaaggtt ttcatacaga gatactcttc
     5641 ataagatacc tgaagcctgt gtaactcaga ggaaacatac agcatgtgtt tacattggtc
     5701 gtacatgcag ggtagagtca ttctctgctc attaataatc agatcaggag caaaacacag
     5761 caggtttgca cttgattgtc tatatgatct ccaccccaga gcaaatgcca taagaaacat
     5821 ccaggagtac tgcagtaggg tcatttggtc atccaggtgt aagttcctga aacctggtat
     5881 tgcctttgcc catttcactg ctgcaatcac ttgccgccct cctaacatgt tgagcgtagt
     5941 catgatcctc caagttgagt ctggaacaga gctatcatat cctgcatata acacttcagg
     6001 ttcaataacc tccaacagtg acaccagggt aggggtgagt tgtggtaacg ttgcaggaac
     6061 tattgttttg ttaccaggat tttcagaggt ttcttgtgag actcctgtag tggcctgctg
     6121 aattcctttt atttttttct ttgtttttcg agcttccagg ttcattccag cctgaagaca
     6181 ttttcgatag cggcatgctg ggcagttttt tcttcgaatt ttatcgatga tgcaatcatt
     6241 ccttccagca cataggtaat tgtgctgtct accttccact gctcttttga agaaaacttt
     6301 acagcttcca caagttaaga ctccataatg acatcctgaa gcttcatcag agcacaccag
     6361 gcagagtttg ggaggtggtc ctgttgttgc tgttgaggag ctggatggag gagagcttac
     6421 atctggtctc atgctggggc ttgaatagcc attagaaaaa actgttcgac cagggaagtt
     6481 cagagtcccc agagaagtca agttgtcatc tccagatcct tggcacctat tccaattttc
     6541 ggaaccaacg ggaattggtg gaatgacatt aaaaataggc ttctgatcct gctgttgaga
     6601 aagggatgct gtattcatgt catagtggta catctgtcct ccagaggtac tcacaccatg
     6661 aacagaaatg gcagacattt tattaccaat tatatttgct ccaggaaagc ttgcctgaca
     6721 gtaaactgtg cccagtttct cttgcttaat taccccaggg gtgcagagtt cgatgaaatc
     6781 ttctttttct gttttcactt ggggcagtgt tacattactg gggcttgaca aaaccagatc
     6841 tccattatcc ttaattttgg gtttagtgtc cggtaaaatg agaggcttgc agtcctcatt
     6901 cgagtttcct tccaaaagga atgaatcgtc ttctcccgcc agaggagaaa gcaaacagtt
     6961 ttcatctatc aacaggtctg atctccaagg actctcattc gtctctttac ctggggaccc
     7021 agaagaaaac tccaaatcct gcaaaatgtc aaaggtgctt tggtctgtgg tatacaattt
     7081 cacattgcca ccgttggtgc cagtctggcc cttcaaatgt tgctgttctg aagatacatc
     7141 agagtgagtt tttggaaact ccttctctgt gggggcagca gacacagcag tggatgctga
     7201 actcttgggg ttctctggaa cactggtcga cctattgagg tttgcaatgc tttcttccaa
     7261 aagctttaag tctgtttccc ccgaggaaag gctgatttgg ccctgctgtg ggaatcccag
     7321 gtcatttccc atcacttttg tttctgtctc tcccatatac agtcccattg agagtgaaac
     7381 tgctttggac agatctggct gctgcgcatt gcttactgag ccttttggaa aatcaaccaa
     7441 aagtcttcgc tgcttggagt ctgattgaga agcgacagcc agtgagggtg aagacgcaga
     7501 aaccttcaca gtagctcctc ctcttagggt tttatagaag tccatcacat ctcccctctc
     7561 ctgagcaagc acactgctgg ggttttcttc tctaccagga gttaatgatt ctttggagtc
     7621 catttcgaac cgcgggctag cggatccgtc gacggtatcg ataagcttga ttcgaccggc
     7681 cgactcagtc tgtcggagga ctggcgcgcc gagtgagggg ttgtgagctc ttttatagag
     7741 ctcgggaagc agaagcgcgc gaacagaagc gagaagcagg ctgattggtt aattcaaata
     7801 aggcgcaggg tcatttcagg tccttggggg agcctggaaa catctgatgg gtcttaagaa
     7861 actgctgagg gttgggccat atctggggac catctgttct tggcctcggg ccggggccga
     7921 aactgcggtg accatctgtt cttggccccg ggccggggcc gaaactgctc accgcagata
     7981 tcctgtttgg cccaacgtta gctattttca tgtacccgcc cttgatctga acttctctat
     8041 tcttggtttg gtatttttcc atgccttgca aaatggcgtt actgcagcca gctcgaggct
     8101 agtctcgtga tcgataaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg
     8161 tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc
     8221 ttttctttta aaagttaacc gataccgtcg agatccgttc actaatcgaa tggatctgtc
     8281 tctgtctctc tctccacctt cttcttctat tccttcgggc ctgtcgggtc ccctcggggt
     8341 tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat tcactataga
     8401 aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta tgaataattt
     8461 tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc atttatctaa
     8521 ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa ttaaggagtg
     8581 tattaagctt gtgtaattgt taatttctct gtcccactcc atccaggtcg tgtgattcca
     8641 aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag tggtgcaaat
     8701 gagttttcca gagcaacccc aaatccccag gagctgttga tcctttaggt atctttccac
     8761 agccaggatt cttgcctgga gctgcttgat gccccagact gtgagttgca acagatgctg
     8821 ttgcgcctca atagccctca gcaaattgtt ctgctgctgc actataccag acaataattg
     8881 tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa
     8941 gaacccaagg aacaaagctc ctattcccac tgctc
//
