LOCUS       sequence-450431-        6976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor ID3 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6976)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-ID3-Brd 193
FEATURES             Location/Qualifiers
     source          1..6976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1809..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1960..2366
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    2980..3383
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             3502..3735
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3807..3928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3844..3863)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3898..3917
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3968..4103
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4123..4142)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4124..4142)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4152..4169)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4152..4168)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4161..4183)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4310..4765
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4397..4416)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4607..4628
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4791..4895
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4896..4964,4965..5756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5114..5133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5927..6515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6416..6435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6669..6686
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6803..6824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6839..6844,6845..6862,6863..6869)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6877..6893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6882..6904
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6901..6917
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6901..6917
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6936..6956
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6938..6956
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1921 aattttatcg atcacgagac tagcctcgag ctggctgcag taacgccatt ttgcaaggca
     1981 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2041 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2101 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2161 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2221 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2281 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2341 gtcctccgac agactgagtc ggccggtcga atcaagctta tcgataccgt cgacgccacc
     2401 atgaaggctc tgagccctgt gcggggatgt tatgaggccg tgtgttgcct gagcgagcgg
     2461 agccttgcta tcgccagagg aagaggaaaa ggacctgccg ccgaggaacc tctgagcctg
     2521 ctggacgata tgaaccactg ctacagcaga ctgcgcgagc tggttcctgg cgtgccaaga
     2581 ggaacacagc tgagccaggt ggaaatcctg cagagagtga tcgactacat cctggatctg
     2641 caggtcgtgc tggccgaacc tgctcctgga cctcctgatg gacctcatct gcctatccag
     2701 accgccgagc tgacacctga gctggtcatc agcaacgaca agcggagctt ctgtcactga
     2761 taaggatccc ccgggctgca ggaattcgag catcttaccg ccatttattc ccatatttgt
     2821 tctgtttttc ttgatttggg tatacattta aatgttaata aaacaaaatg gtggggcaat
     2881 catttacatt tttagggata tgtaattact agttcaggtg tattgccaca agacaaacat
     2941 gttaagaaac tttcccgtta tttacgctct gttcctgtta atcaacctct ggattacaaa
     3001 atttgtgaaa gattgactga tattcttaac tatgttgctc cttttacgct gtgtggatat
     3061 gctgctttaa tgcctctgta tcatgctatt gcttcccgta cggctttcgt tttctcctcc
     3121 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt ccgtcaacgt
     3181 ggcgtggtgt gctctgtgtt tgctgacgca acccccactg gctggggcat tgccaccacc
     3241 tgtcaactcc tttctgggac tttcgctttc cccctcccga tcgccacggc agaactcatc
     3301 gccgcctgcc ttgcccgctg ctggacaggg gctaggttgc tgggcactga taattccgtg
     3361 gtgttgtcgg ggaagctgac gtcctttgca attcgatatc aagctgtacc ttctagatgt
     3421 acatgtttat tagcgcttct agaccaatga cttacaaggc agctgtagat cttagccact
     3481 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     3541 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     3601 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     3661 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     3721 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     3781 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     3841 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3901 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     3961 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4021 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     4081 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     4141 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     4201 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     4261 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     4321 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4381 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4441 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4501 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4561 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4621 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4681 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4741 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4801 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4861 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4921 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4981 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5041 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5101 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5161 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5221 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5281 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5341 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5401 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5461 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5521 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5581 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5641 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5701 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5761 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5821 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5881 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5941 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6001 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6061 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6121 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6181 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6241 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6301 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6361 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6421 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6481 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6541 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6601 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6661 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6721 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6781 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     6841 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     6901 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     6961 aaaagctgga gctgca
//
