LOCUS       sequence-450436-        9097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor HIF1A with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9097)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 9097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-HIF1A-Brd 190
FEATURES             Location/Qualifiers
     source          1..9097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1809..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1960..2366
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2395..2404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5101..5504
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             5623..5856
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5928..6049
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5965..5984)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6019..6038
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6089..6224
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6244..6263)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6245..6263)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6273..6290)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6273..6289)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6282..6304)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6431..6886
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6518..6537)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6728..6749
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6912..7016
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7017..7085,7086..7877)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7235..7254)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8048..8636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8537..8556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8790..8807
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8924..8945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8960..8965,8966..8983,8984..8990)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8998..9014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9003..9025
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9022..9038
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9022..9038
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9057..9077
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9059..9077
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1921 aattttatcg atcacgagac tagcctcgag ctggctgcag taacgccatt ttgcaaggca
     1981 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2041 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2101 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2161 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2221 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2281 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2341 gtcctccgac agactgagtc ggccggtcga atcaagctta tcgataccgt cgacgccacc
     2401 atggaaggtg ccggcggagc caacgacaag aagaagatca gcagcgagcg gcggaaagag
     2461 aagtctaggg acgctgccag aagcaggcgg agcaaagaaa gcgaggtgtt ctacgagctg
     2521 gcccaccaac tgcctctgcc tcacaatgtg tccagccacc tggataaggc cagcgtgatg
     2581 agactgacca tcagctacct gagagtgcgg aagctgctgg atgccggcga tctggacatc
     2641 gaggacgata tgaaggccca gatgaactgc ttctacctga aggccctgga cggcttcgtg
     2701 atggtgctga ccgatgacgg cgacatgatc tacatcagcg acaacgtgaa caagtacatg
     2761 gggctgaccc agttcgagct gacaggccac agcgtgttcg acttcacaca cccctgcgac
     2821 cacgaagaga tgagagagat gctgacccac cggaacggcc tggtcaagaa gggcaaagag
     2881 cagaataccc agcggtcatt cttcctgcgg atgaagtgca ccctgaccag caggggcaga
     2941 accatgaaca tcaagagcgc cacatggaaa gtgctgcact gcaccggaca catccacgtg
     3001 tacgacacca acagcaacca gcctcagtgc ggctacaaga aacctcctat gacctgcctg
     3061 gtgctgatct gcgagcccat tcctcatcct agcaacatcg agatccctct ggacagcaag
     3121 accttcctga gcagacacag cctggacatg aagttcagct actgcgacga gcggatcacc
     3181 gagctgatgg gctatgagcc tgaagaactg ctgggccgca gcatctacga gtactatcac
     3241 gccctggaca gcgaccacct gaccaagaca caccacgaca tgttcaccaa gggccaagtg
     3301 accaccggcc agtacagaat gctggccaag cgcggaggct acgtgtgggt tgaaacacag
     3361 gccaccgtga tctacaacac caagaactcc cagccacagt gcatcgtgtg cgtgaactac
     3421 gtggtgtccg gcatcatcca gcacgacctg atcttcagcc tgcaacagac cgagtgcgtg
     3481 ctgaagcctg tggaaagcag cgacatgaag atgacccagc tgtttaccaa ggtggaatcc
     3541 gaggacacca gcagcctgtt cgacaagctg aagaaagagc ccgacgctct gacactgctg
     3601 gctccagctg ctggcgatac catcatcagc ctggatttcg gcagcaacga caccgagaca
     3661 gacgaccagc agctcgagga agtgcccctg tacaacgacg tgatgctgcc ctctccaaac
     3721 gagaagctcc agaacatcaa cctggccatg tcacccctgc ctaccgccga gacacctaag
     3781 cctctgagaa gctctgccga tcctgctctg aatcaagagg tggccctgaa gctggaaccc
     3841 aatcctgaga gcctggaact gagcttcacc atgcctcaga tccaggatca gaccccatct
     3901 cctagcgacg gcagcacaag acagtctagc cccgagccta atagccccag cgagtactgc
     3961 ttttacgtgg actccgacat ggtcaacgag ttcaaactgg aactggtcga gaagctgttc
     4021 gccgaggata ccgaggccaa gaatcccttc agcacccaag acaccgacct ggacctggaa
     4081 atgctggcac cttacatccc tatggacgac gacttccagc tgcggagctt cgatcagctg
     4141 agccctctgg aatctagcag cgcctctcca gagtctgcct ctccacagag caccgtgacc
     4201 gtgtttcagc agacccagat ccaagagcca accgccaatg ccaccaccac aaccgccaca
     4261 acagacgagc tgaaaaccgt gaccaaggac cggatggaag atatcaagat cctgatcgct
     4321 agcccctctc ctactcacat ccacaaagag acaaccagcg ccacaagcag cccctacaga
     4381 gacacccaga gcagaaccgc ctctcctaac agagccggca aaggcgtgat cgagcagacc
     4441 gagaagtctc accctagaag ccccaacgtg ctgagcgtgg cactgagcca gagaaccaca
     4501 gtgcccgagg aagaactgaa ccccaagatt ctggccctcc agaacgccca gcggaagcgg
     4561 aaaatggaac acgacggaag cctgttccag gccgtcggaa tcggaacact gctccagcag
     4621 ccagatgatc acgccgccac cacaagcctg agctggaaga gagtgaaggg ctgcaagtcc
     4681 agcgagcaga acggaatgga acagaaaacc atcattctga tccccagcga cctggcctgt
     4741 agactgctcg gccaatctat ggatgagagc ggcctgcctc agctgaccag ctacgactgc
     4801 gaagtgaacg cccctatcca gggcagcaga aacctgctcc aaggcgagga actgctgcgc
     4861 gctctggacc aagtgaactg ataaggatcc cccgggctgc aggaattcga gcatcttacc
     4921 gccatttatt cccatatttg ttctgttttt cttgatttgg gtatacattt aaatgttaat
     4981 aaaacaaaat ggtggggcaa tcatttacat ttttagggat atgtaattac tagttcaggt
     5041 gtattgccac aagacaaaca tgttaagaaa ctttcccgtt atttacgctc tgttcctgtt
     5101 aatcaacctc tggattacaa aatttgtgaa agattgactg atattcttaa ctatgttgct
     5161 ccttttacgc tgtgtggata tgctgcttta atgcctctgt atcatgctat tgcttcccgt
     5221 acggctttcg ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     5281 tggcccgttg tccgtcaacg tggcgtggtg tgctctgtgt ttgctgacgc aacccccact
     5341 ggctggggca ttgccaccac ctgtcaactc ctttctggga ctttcgcttt ccccctcccg
     5401 atcgccacgg cagaactcat cgccgcctgc cttgcccgct gctggacagg ggctaggttg
     5461 ctgggcactg ataattccgt ggtgttgtcg gggaagctga cgtcctttgc aattcgatat
     5521 caagctgtac cttctagatg tacattcttc ccagcgcttc tagaccaatg acttacaagg
     5581 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5641 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     5701 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5761 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5821 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     5881 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     5941 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6001 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     6061 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     6121 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     6181 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     6241 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     6301 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     6361 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     6421 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6481 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6541 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6601 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6661 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6721 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6781 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6841 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     6901 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6961 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7021 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7081 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7141 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7201 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7261 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7321 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     7381 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     7441 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7501 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7561 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7621 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     7681 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7741 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7801 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7861 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7921 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7981 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8041 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8101 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8161 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8221 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8281 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8341 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8401 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8461 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8521 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8581 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8641 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8701 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     8761 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     8821 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     8881 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     8941 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     9001 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     9061 ttaaccctca ctaaagggaa caaaagctgg agctgca
//
