LOCUS       sequence-450440-        7885 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor GFI1 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7885)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 7885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-GFI1-Brd 188
FEATURES             Location/Qualifiers
     source          1..7885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..235
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             236..416
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    463..588
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1081..1314
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1499..1543
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1810..1927
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1961..2367
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    3890..4293
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             4412..4645
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4717..4838
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4754..4773)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4808..4827
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4878..5013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5033..5052)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5034..5052)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5062..5079)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5062..5078)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5071..5093)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5220..5675
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5307..5326)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5517..5538
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5701..5805
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5806..5874,5875..6666)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6024..6043)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6837..7425
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7326..7345
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7579..7596
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7713..7734
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7749..7754,7755..7772,7773..7779)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7787..7803
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7792..7814
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7811..7827
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7811..7827
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7846..7866
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7848..7866
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     1921 aaattttatc gatcacgaga ctagcctcga gctggctgca gtaacgccat tttgcaaggc
     1981 atggaaaaat accaaaccaa gaatagagaa gttcagatca agggcgggta catgaaaata
     2041 gctaacgttg ggccaaacag gatatctgcg gtgagcagtt tcggccccgg cccggggcca
     2101 agaacagatg gtcaccgcag tttcggcccc ggcccgaggc caagaacaga tggtccccag
     2161 atatggccca accctcagca gtttcttaag acccatcaga tgtttccagg ctcccccaag
     2221 gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc agcctgcttc tcgcttctgt
     2281 tcgcgcgctt ctgcttcccg agctctataa aagagctcac aacccctcac tcggcgcgcc
     2341 agtcctccga cagactgagt cggccggtcg aatcaagctt atcgataccg tcgacgccac
     2401 catgcctaga tccttcctgg tcaagagcaa gaaggcccac agctaccacc agcctagaag
     2461 ccccggacct gactacagcc tgagactgga aaatgtgccc gctcctagca gagccgacag
     2521 cacatctaat gccggcggag ccaaagccga gcctagagat agactgagcc ctgagagcca
     2581 gctgacagag gcccctgata gagcctctgc ctctcctgat agctgcgagg gatctgtgtg
     2641 cgagagaagc agcgagttcg aggacttttg gcggcctcca tctcctagcg cctctccagc
     2701 ctctgagaag tccatgtgtc ccagcctgga tgaggcccag ccttttccac tgcctttcaa
     2761 gccctactct tggagcggac tggccggctc tgatctgaga catctggtgc agagctacag
     2821 accctgtggc gcccttgaaa gaggtgctgg ccttggcctg ttttgcgagc ctgctcctga
     2881 acctggacat cccgctgctc tgtacggacc taaaagagca gctggcggag ctggtgctgg
     2941 tgcccctgga tcttgttctg ctggtgcagg cgcaacagct ggacctggat tgggactgta
     3001 cggcgatttt ggatctgccg ccgctggcct gtacgaaaga cctacagctg ctgccggcct
     3061 gctgtatcct gaaagaggac acggactgca cgccgacaaa ggcgctggcg tgaaggtgga
     3121 atctgagctg ctgtgcacca gactgctgct cggcggaggc agctacaagt gcatcaagtg
     3181 cagcaaggtg ttcagcaccc ctcacggcct ggaagtgcat gtgcggagat ctcacagcgg
     3241 caccagacct tttgcctgcg agatgtgcgg caagaccttt ggccatgccg tgtctctgga
     3301 acagcacaag gccgtgcata gccaagagcg gagcttcgac tgcaagatct gtggcaagag
     3361 cttcaagcgg agcagcaccc tgagcaccca tctgctgatc cactccgaca ccaggcctta
     3421 tccttgccag tattgcggca agcggttcca ccagaaaagc gacatgaaga agcacacctt
     3481 catccacacc ggcgagaagc cccacaagtg tcaagtgtgc ggaaaggcct tcagccagtc
     3541 cagcaatctg atcacccaca gcagaaagca caccggcttc aagcctttcg gctgcgacct
     3601 gtgtggcaag ggattccaga gaaaggtgga cctgcggcgg cacagagaaa cacagcacgg
     3661 actgaagtga taaggatccc ccgggctgca ggaattcgag catcttaccg ccatttattc
     3721 ccatatttgt tctgtttttc ttgatttggg tatacattta aatgttaata aaacaaaatg
     3781 gtggggcaat catttacatt tttagggata tgtaattact agttcaggtg tattgccaca
     3841 agacaaacat gttaagaaac tttcccgtta tttacgctct gttcctgtta atcaacctct
     3901 ggattacaaa atttgtgaaa gattgactga tattcttaac tatgttgctc cttttacgct
     3961 gtgtggatat gctgctttaa tgcctctgta tcatgctatt gcttcccgta cggctttcgt
     4021 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     4081 ccgtcaacgt ggcgtggtgt gctctgtgtt tgctgacgca acccccactg gctggggcat
     4141 tgccaccacc tgtcaactcc tttctgggac tttcgctttc cccctcccga tcgccacggc
     4201 agaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctaggttgc tgggcactga
     4261 taattccgtg gtgttgtcgg ggaagctgac gtcctttgca attcgatatc aagctgtacc
     4321 ttctagatgt acaagggtac gagcgcttct agaccaatga cttacaaggc agctgtagat
     4381 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     4441 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     4501 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     4561 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     4621 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     4681 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     4741 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     4801 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     4861 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4921 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     4981 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     5041 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     5101 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     5161 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     5221 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     5281 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     5341 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     5401 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     5461 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     5521 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     5581 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     5641 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     5701 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5761 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5821 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5881 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5941 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6001 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     6061 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6121 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6181 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6241 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6301 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     6361 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6421 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6481 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6541 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6601 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6661 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6721 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6781 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6841 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6901 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6961 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7021 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7081 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7141 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7201 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7261 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7321 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7381 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7441 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7501 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7561 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7621 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7681 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7741 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7801 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     7861 taaagggaac aaaagctgga gctgc
//
