LOCUS       sequence-450444-        7459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor HES1 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7459)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 7459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-HES1-Brd 189
FEATURES             Location/Qualifiers
     source          1..7459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1809..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1960..2366
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    3463..3866
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             3985..4218
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4290..4411
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4327..4346)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4381..4400
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4451..4586
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4606..4625)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4607..4625)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4635..4652)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4635..4651)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4644..4666)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4793..5248
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5090..5111
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5274..5378
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5379..5447,5448..6239)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5597..5616)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6410..6998
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6899..6918
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7152..7169
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7286..7307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7322..7327,7328..7345,7346..7352)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7360..7376
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7365..7387
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7384..7400
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7384..7400
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7419..7439
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7421..7439
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1921 aattttatcg atcacgagac tagcctcgag ctggctgcag taacgccatt ttgcaaggca
     1981 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2041 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2101 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2161 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2221 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2281 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2341 gtcctccgac agactgagtc ggccggtcga atcaagctta tcgataccgt cgacgccacc
     2401 atgcccgccg acatcatgga aaagaacagc agcagccccg tggccgctac tcctgcttct
     2461 gtgaacacca cacctgacaa gcccaagacc gccagcgagc acagaaagtc cagcaagccc
     2521 attatggaaa agcggcggag agcccggatc aacgagtctc tgagccagct gaaaaccctg
     2581 atcctggacg ccctgaagaa ggacagctcc agacacagca agctggaaaa ggccgatatc
     2641 ctggaaatga ccgtgaagca cctgagaaac ctccagcggg cccagatgac agccgctctg
     2701 tctacagatc ctagcgtgct gggcaagtac agagccggct tcagcgagtg catgaacgaa
     2761 gtgacccggt tcctgagcac atgcgagggc gtgaacacag aagtgcggac aagactgctg
     2821 ggccacctgg ccaattgcat gacccagatc aacgccatga cataccccgg ccagcctcat
     2881 cctgcactgc aagctcctcc acctccacca cctggacctg gtggacctca gcatgctcct
     2941 ttcgcaccac ctcctccact ggtgccaatt ccaggcggag ctgctccacc gcctggcgga
     3001 gcaccttgta aactgggatc tcaggctggc gaggccgcca aagtgtttgg cggatttcag
     3061 gtggtgcccg ctcctgatgg ccagttcgcc tttctgatcc ccaatggcgc cttcgctcac
     3121 agcggacctg tgatccctgt gtacaccagc aatagcggca caagcgtggg ccccaatgcc
     3181 gtgtctccat cttctggacc tagcctgacc gccgattcta tgtggcggcc ttggagaaac
     3241 tgataaggat cccccgggct gcaggaattc gagcatctta ccgccattta ttcccatatt
     3301 tgttctgttt ttcttgattt gggtatacat ttaaatgtta ataaaacaaa atggtggggc
     3361 aatcatttac atttttaggg atatgtaatt actagttcag gtgtattgcc acaagacaaa
     3421 catgttaaga aactttcccg ttatttacgc tctgttcctg ttaatcaacc tctggattac
     3481 aaaatttgtg aaagattgac tgatattctt aactatgttg ctccttttac gctgtgtgga
     3541 tatgctgctt taatgcctct gtatcatgct attgcttccc gtacggcttt cgttttctcc
     3601 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtccgtcaa
     3661 cgtggcgtgg tgtgctctgt gtttgctgac gcaaccccca ctggctgggg cattgccacc
     3721 acctgtcaac tcctttctgg gactttcgct ttccccctcc cgatcgccac ggcagaactc
     3781 atcgccgcct gccttgcccg ctgctggaca ggggctaggt tgctgggcac tgataattcc
     3841 gtggtgttgt cggggaagct gacgtccttt gcaattcgat atcaagctgt accttctaga
     3901 tgtacattta gttgagcgct tctagaccaa tgacttacaa ggcagctgta gatcttagcc
     3961 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     4021 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     4081 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     4141 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     4201 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     4261 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     4321 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4381 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     4441 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4501 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4561 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     4621 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4681 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4741 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     4801 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4861 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4921 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4981 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5041 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5101 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5161 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5221 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     5281 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5341 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5401 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5461 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5521 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5581 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5641 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5701 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5761 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5821 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5881 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5941 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6001 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6061 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6121 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6181 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6241 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6301 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6361 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6421 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6481 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6541 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6601 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6661 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6721 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6781 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6841 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6901 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6961 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7021 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7081 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7141 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7201 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     7261 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     7321 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     7381 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     7441 aacaaaagct ggagctgca
//
