LOCUS       sequence-450446-       16722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac cargo vector encoding PEmax and mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16722)
  AUTHORS   Loveless BT, Carlson CK, Dentzel Helmy CA, Hu VJ, Ross SK, Demelo 
            MC, Murtaza A, Liang G, Ficht M, Singhai A, Pajoh-Casco MJ, Liu CC
  TITLE     Open-ended molecular recording of sequential cellular events into 
            DNA
  JOURNAL   bioRxiv 2021.11.05.467507
REFERENCE   2  (bases 1 to 16722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.11.05.467507"
COMMENT     Sequence Label: pTBL3478 PB-PEmax-mTagBFP2
FEATURES             Location/Qualifiers
     source          1..16722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        922..1225
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1506..2522
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2446..2468
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2530..2549
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2619..2639
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2640..6740
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6798..6818
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8919..8939
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8949..8975
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     polyA_signal    9002..9226
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        9502..10713
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     10550..10569
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             10720..11418
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             11428..11448
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(11542..11561)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    11607..11662
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(11661..11680)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        12709..12912
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12863..12883
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      12934..12943
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12940..13968
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             12940..13968
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     polyA_signal    13986..14193
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   14400..14462
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(14513..14531)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(14571..15386)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(14571..15386)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     15294..15313
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     15378..15397
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      15611..16199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16100..16119
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16311..16330
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16311..16329
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   16359..16393
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ctgaactggc cgataattgc agacgatcga attcgagttg gcgcgcctgt cattctaaat
       61 ctctctttca gcctaaagct ttttccccgt atccccccag gtgtctgcag gctcaaagag
      121 cagcgagaag cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc
      181 ccgcacgctg ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg
      241 gctcgctgct gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggagg
      301 gggctgtccc cgtgagctcc caggcgcgcc tgtcattcta aatctctctt tcagcctaaa
      361 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
      421 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
      481 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
      541 agcgggggag ggacgtaatt acatccctgg gggctttggg agggggctgt ccccgtgagc
      601 tcccaggcgc gcctgtcatt ctaaatctct ctttcagcct aaagcttttt ccccgtatcc
      661 ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg
      721 ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg cggggggagc
      781 gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg gagggacgta
      841 attacatccc tgggggcttt gggggggggc tgtccccgtg agctcaataa aagagcccac
      901 aacgccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      961 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1021 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1081 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1141 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1201 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     1261 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     1321 agcgatgggg gcgggggggg gggggggggc gcgcgcsggg gggggggggg gggggsgggg
     1381 ggsggggsgg ggsgrggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
     1441 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
     1501 gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc
     1561 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
     1621 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
     1681 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg
     1741 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg
     1801 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg
     1861 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg
     1921 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc
     1981 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
     2041 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggkgggg gtgccgggcg
     2101 gggcggggcc gccycgggcc ggggrgggct cgggggrggg gcgcggcggc ccccggagtg
     2161 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     2221 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     2281 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     2341 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     2401 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     2461 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     2521 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattcgcca
     2581 ctatgaaacg gacagccgac ggaagcgagt tcgagtcacc aaagaagaag cggaaagtcg
     2641 acaagaagta cagcatcggc ctggacatcg gcaccaactc tgtgggctgg gccgtgatca
     2701 ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca
     2761 gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca
     2821 cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc
     2881 tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg
     2941 aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca
     3001 tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac
     3061 tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga
     3121 tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg
     3181 acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca
     3241 acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagaaagc
     3301 tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggaaacctga
     3361 ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg
     3421 ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga
     3481 tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc
     3541 tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga
     3601 tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc
     3661 agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct
     3721 acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa
     3781 agatggacgg caccgaggaa ctgctcgtga agctgaagag agaggacctg ctgcggaagc
     3841 agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca
     3901 ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga
     3961 agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat
     4021 tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg
     4081 tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc
     4141 tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata
     4201 acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg
     4261 gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga
     4321 agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg
     4381 gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca
     4441 aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga
     4501 ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc
     4561 tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc
     4621 tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt
     4681 tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc
     4741 tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg
     4801 agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga
     4861 aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg
     4921 aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga
     4981 agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg
     5041 aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata
     5101 tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gacgctatcg
     5161 tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc agaagcgaca
     5221 agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact
     5281 actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca
     5341 aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg
     5401 tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta
     5461 agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc
     5521 tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc
     5581 accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc
     5641 ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga
     5701 tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca
     5761 tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc
     5821 tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca
     5881 ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga
     5941 caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca
     6001 gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt
     6061 ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag
     6121 agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc
     6181 tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact
     6241 ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga
     6301 agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact
     6361 atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc
     6421 acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc
     6481 tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca
     6541 tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg
     6601 ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg
     6661 tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc
     6721 tgtctcagct gggaggtgac tccggcggaa gctctggtgg cagcaagcgg accgccgacg
     6781 gctctgaatt cgagagccct aagaagaaaa gaaaggtgag cggaggctct agcggcggaa
     6841 gcaccctgaa cattgaagac gagtatagac tgcatgaaac aagcaaggaa cccgacgtgt
     6901 ccctgggctc cacctggctg tccgactttc cccaggcctg ggccgagaca ggaggaatgg
     6961 gcctggccgt gcggcaggca cccctgatca tccctctgaa ggccacctct acacccgtga
     7021 gcatcaagca gtaccctatg tctcaggagg ccagactggg catcaagcct cacatccaga
     7081 ggctgctgga ccagggcatc ctggtgccat gccagagccc ctggaacaca ccactgctgc
     7141 ccgtgaagaa gccaggcacc aatgactata gacccgtgca ggatctgaga gaggtgaaca
     7201 agagggtgga ggatatccac cccaccgtgc ccaaccctta caatctgctg tccggcctgc
     7261 ccccttctca ccagtggtat acagtgctgg acctgaagga tgccttcttt tgtctgagac
     7321 tgcaccctac cagccagcca ctgttcgcct ttgagtggag ggaccctgag atgggcatct
     7381 ctggccagct gacctggaca cgcctgcctc agggcttcaa gaatagccca acactgttta
     7441 acgaggccct gcaccgcgac ctggcagatt tccggatcca gcacccagat ctgatcctgc
     7501 tgcagtacgt ggacgatctg ctgctggccg ccaccagcga gctggattgc cagcagggaa
     7561 cacgcgccct gctgcagacc ctgggaaacc tgggatatag ggcatccgcc aagaaggccc
     7621 agatctgtca gaagcaggtg aagtacctgg gctatctgct gaaggagggc cagagatggc
     7681 tgacagaggc caggaaggag acagtgatgg gccagccaac acccaagacc ccaagacagc
     7741 tgagggagtt cctgggcaaa gcaggatttt gcaggctgtt catcccagga ttcgcagaga
     7801 tggcagcacc tctgtaccca ctgaccaagc cgggcaccct gtttaattgg ggccctgacc
     7861 agcagaaggc ctatcaggag atcaagcagg ccctgctgac agcaccagcc ctgggcctgc
     7921 cagacctgac caagcctttc gagctgtttg tggatgagaa gcagggctac gccaagggcg
     7981 tgctgaccca gaagctggga ccatggaggc ggcccgtggc ctatctgtcc aagaagctgg
     8041 acccagtggc agcaggatgg ccaccatgcc tgaggatggt ggcagcaatc gccgtgctga
     8101 caaaggatgc cggcaagctg accatgggac agccactggt catcctggca ccacacgcag
     8161 tggaggccct ggtgaagcag cctccagatc gctggctgtc taacgcccgg atgacacact
     8221 accaggccct gctgctggac accgatcgcg tgcagtttgg ccctgtggtg gccctgaatc
     8281 cagccaccct gctgcctctg ccagaggagg gcctgcagca caactgtctg gacatcctgg
     8341 cagaggcaca cggaacaagg ccagacctga ccgatcagcc cctgcctgac gccgatcaca
     8401 catggtatac cgatggaagc tccctgctgc aggagggcca gaggaaggca ggagcagcag
     8461 tgaccacaga gacagaagtg atctgggcca aggccctgcc agcaggcaca tccgcccagc
     8521 gggccgagct gatcgccctg acccaggccc tgaagatggc cgagggcaag aagctgaacg
     8581 tgtacacaga ctccagatat gccttcgcca ccgcacacat ccacggagag atctacaggc
     8641 gccggggctg gctgacctct gagggcaagg agatcaagaa caaggatgag atcctggccc
     8701 tgctgaaggc cctgtttctg cccaagcggc tgagcatcat ccactgtcct ggacaccaga
     8761 agggacactc cgccgaggca aggggcaatc ggatggccga ccaggccgcc agaaaggctg
     8821 ctattactga aactcccgac acttccactc tgctgattga aaactcctcc ccttctggcg
     8881 gctcaaaaag aaccgccgac ggcagcgaat tcgagtctcc caagaagaag aggaaagtcg
     8941 gctctggccc tgccgctaag agagtgaagc tggacggatc ctaactcgag cgacgcctcg
     9001 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     9061 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     9121 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     9181 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatgggctg aagtgaataa
     9241 agctccacac agtcgatgtt tctatatgtt ttaagtctta ggcatatcgg acatcactag
     9301 tgcatcagcg ccctctgctg gttcagtaag aatggtttcc ccatatactg ggcaaaactg
     9361 gatttttgtg gtgatgaaag ggaaaaaaac aaattcagta caattggaaa gctggtgttg
     9421 ttttaaaact cttgaaaaac actgaatgaa aggaatcaca ctaaaactat atgttgcaat
     9481 gttgttggtt aaaacggatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc
     9541 ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt
     9601 ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt
     9661 atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc
     9721 cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc
     9781 cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc
     9841 cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg
     9901 tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac
     9961 ggaagcgtgt ggagacaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg
    10021 aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga
    10081 cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag
    10141 aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct
    10201 ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc
    10261 gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg
    10321 agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg
    10381 ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc
    10441 ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg
    10501 aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg
    10561 ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt
    10621 gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct
    10681 aaattctggc cgtttttggc ttttttgtta gacgccacta tgagcgagct gattaaggag
    10741 aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt caagtgcaca
    10801 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag
    10861 ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag
    10921 accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc
    10981 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
    11041 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttcaca
    11101 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac cgaaacgctg
    11161 taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg
    11221 agccatctga tcgcaaacat caagaccaca tatagatcca agaaacccgc taagaacctc
    11281 aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga ggccaacaac
    11341 gaaacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct ccctagcaaa
    11401 ctggggcaca agcttaatgg tggaggtcca aaaaagaaaa ggaaggtatc ctaactcgag
    11461 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
    11521 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa
    11581 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
    11641 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
    11701 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
    11761 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
    11821 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
    11881 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
    11941 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca
    12001 gcccaagctt ggcgtaatgc tggaaatctg ctcgtcagtg gtgctcacac tgacgaatca
    12061 tgtacagatc ataccgatga ctgcctggcg actcacaact aagcaagaca gccggaacca
    12121 gcgccggcga acaccactgc atatatggca tatcacaaca gtccaagcag ttacagagat
    12181 gttacgaacc ctgaacaggt tgactattaa ttgtgtctgc ttgatgtgga caccaggtgg
    12241 cgctggacat cagatttgga gaggcagttg tctagggaac cgggctctgt gccagcgcag
    12301 gaggcaggct ggctctccta ttccagggat gctcatccag gaaggaaagg ttgcatgctg
    12361 gacacactaa ccttacagcg gtgggctcta tggggtacct acgtaggtac ataacttacg
    12421 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
    12481 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
    12541 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
    12601 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
    12661 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
    12721 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
    12781 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
    12841 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
    12901 ataagcagag ctcgtttagt gaaccgtcag atagccacca tgggtaaaaa gcctgaactc
    12961 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtgtc cgacctgatg
    13021 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
    13081 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
    13141 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt tagcgagagc
    13201 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
    13261 gaactgcccg ctgttctgca accggtcgcg gaggccatgg atgcgatcgc tgcggccgat
    13321 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
    13381 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
    13441 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
    13501 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
    13561 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
    13621 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
    13681 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg
    13741 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
    13801 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
    13861 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
    13921 gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaataact cgagcgacgc
    13981 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
    14041 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
    14101 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
    14161 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatga gtatcgatac
    14221 tagttaaaag ttttgttact ttatagaaga aattttgagt ttttgttttt ttttaataaa
    14281 taaataaaca taaataaatt gtttgttgaa tttattatta gtatgtaagt gtaaatataa
    14341 taaaacttaa tatctattca aattaataaa taaacctcga tatacagacc gataaaacac
    14401 atgcgtcaat tttacgcatg attatcttta acgtacgtca caatatgatt atctttctag
    14461 ggttaatcta gtatacgcgt atgcggccgc ttaattaatc cagcttttgt tccctttagt
    14521 gagggttaat tccgagcggc cgcgtgttac aaccaattaa ccaattctga ttagaaaaac
    14581 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
    14641 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
    14701 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
    14761 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
    14821 gagaatggca aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc
    14881 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
    14941 aggcgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
    15001 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
    15061 acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta
    15121 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
    15181 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
    15241 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga
    15301 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctggagcaa
    15361 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
    15421 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga
    15481 gacacaacgt ggctttgttg aataaatcga acttttgctg agttgaagga tcagtcatga
    15541 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    15601 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    15661 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    15721 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    15781 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    15841 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    15901 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    15961 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    16021 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    16081 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    16141 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    16201 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    16261 tctttcctgc gttatcccct gattctgtgg ataaccgtgc ggccgccaat taatacgact
    16321 cactataggg cgaattgggg cgcgccattc tagattaacc ctagaaagat agtctgcgta
    16381 aaattgacgc atgcattctt gaaatattgc tctctctttc taaatagcgc gaatccgtcg
    16441 ctgtgcattt aggacatctc agtcgccgct tggagctccc gtgaggcgtg cttgtcaatg
    16501 cggtaagtgt cactgatttt gaactataac gaccgcgtga gtcaaaatga cgcatgatta
    16561 tcttttacgt gacttttaag atttaactca tacgataatt atattgttat ttcatgttct
    16621 acttacgtga taacttatta tatatatatt ttcttgttat agatatcaac tagaatgcta
    16681 gcatgggccc agatctccat gggccctgaa ttcgcatcta ga
//
