LOCUS       sequence-450451-        9962 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor ZEB1 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9962)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 9962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-ZEB1-Brd 151
FEATURES             Location/Qualifiers
     source          1..9962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(18..62)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    247..480
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    973..1098
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1145..1325
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1326..1552)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1580..1600)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1580..1598)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1619..1635)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1619..1635)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1632..1654)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1643..1659
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1667..1673,1674..1691,1692..1697))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1712..1733
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1850..1867)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2021..2609)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2101..2120)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2780..3571,3572..3640))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3403..3422
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3641..3745)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3771..4226)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3908..3929)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4120..4139
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4353..4375
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4367..4384
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4368..4384
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4394..4413
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4394..4412
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4433..4568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4608..4729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4619..4638)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4673..4692
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4801..5034
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5153..5556
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     promoter        complement(9156..9562)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    9596..9713
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc ttgttcattc
       61 ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta
      121 atttctctgt cccactccat ccaggtcgtg tgattccaaa tctgttccag agatttatta
      181 ctccaactag cattccaagg cacagcagtg gtgcaaatga gttttccaga gcaaccccaa
      241 atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct tgcctggagc
      301 tgcttgatgc cccagactgt gagttgcaac agatgctgtt gcgcctcaat agccctcagc
      361 aaattgttct gctgctgcac tataccagac aataattgtc tggcctgtac cgtcagcgtc
      421 attgacgctg cgcccatagt gcttcctgct gctcccaaga acccaaggaa caaagctcct
      481 attcccactg ctcttttttc tctctgcacc actcttctct ttgccttggt gggtgctact
      541 cctaatggtt caatttttac tactttatat ttatataatt cacttctcca attgtccctc
      601 atatctcctc ctccaggtct gaagatcagc ggccgcttgc tgtgcggtgg tcttactttt
      661 gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg
      721 atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt
      781 ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag
      841 gccaggatta actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt
      901 atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc
      961 ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat
     1021 ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg
     1081 agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg
     1141 ccactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     1201 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     1261 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
     1321 gacccgttta tgtatcgagc taggcactta aatacaatat ctctgcaatg cggcaattca
     1381 gtggttcgtc caatccatgt cagacccgtc tgttgccttc ctaataaggc acgatcgtac
     1441 caccttactt ccaccaatcg gcatgcacgg tgctttttct ctccttgtaa ggcatgttgc
     1501 taactcatcg ttaccatgtt gcaagactac aagagtattg cataagacta cattaagctt
     1561 gcagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     1621 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     1681 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     1741 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     1801 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     1861 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     1921 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     1981 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2041 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2101 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2161 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2221 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2281 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2341 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2401 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2461 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2521 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2581 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2641 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2701 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2761 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2821 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2881 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     2941 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3001 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3061 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     3121 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3181 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3241 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3301 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3361 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3421 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3481 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3541 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3601 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3661 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3721 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
     3781 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     3841 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     3901 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     3961 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     4021 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     4081 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     4141 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     4201 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
     4261 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     4321 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     4381 cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtacgtccc taggcctcca
     4441 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
     4501 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     4561 ggcgggatgg gcggagttag gggcgggata gctagagcca gacatgataa gatacattga
     4621 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     4681 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttc ctctcactct
     4741 ctgatattca tttctttgca agttataaat actgaataat aagatgacat gaactactac
     4801 tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac
     4861 acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca
     4921 gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc
     4981 cagtacaagc aaaaagcaga tcttgtcttc gttgggagtg aattagccct tccagtcccc
     5041 ccttttcttt taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt ggtctagaag
     5101 cgctaaaagt ggtgtacatc tagaaggtac agcttgatat cgaattgcaa aggacgtcag
     5161 cttccccgac aacaccacgg aattatcagt gcccagcaac ctagcccctg tccagcagcg
     5221 ggcaaggcag gcggcgatga gttctgccgt ggcgatcggg agggggaaag cgaaagtccc
     5281 agaaaggagt tgacaggtgg tggcaatgcc ccagccagtg ggggttgcgt cagcaaacac
     5341 agagcacacc acgccacgtt gacggacaac gggccacaac tcctcataaa gagacagcaa
     5401 ccaggattta tacaaggagg agaaaacgaa agccgtacgg gaagcaatag catgatacag
     5461 aggcattaaa gcagcatatc cacacagcgt aaaaggagca acatagttaa gaatatcagt
     5521 caatctttca caaattttgt aatccagagg ttgattaaca ggaacagagc gtaaataacg
     5581 ggaaagtttc ttaacatgtt tgtcttgtgg caatacacct gaactagtaa ttacatatcc
     5641 ctaaaaatgt aaatgattgc cccaccattt tgttttatta acatttaaat gtatacccaa
     5701 atcaagaaaa acagaacaaa tatgggaata aatggcggta agatgctcga attcctgcag
     5761 cccgggggat ccgaccggtt aggcttcatt tgtcttttct tcagacactt gctcactact
     5821 ctcgcctact ttttgtccta agctgcttgc ttgactttca gccctgtcat ccttcatcag
     5881 accttcagtt tttgcttctt ctccctcatt ctctgcctct tctacctctt cttcttccac
     5941 ttcttcctcc tcctcctcct cttcctcatc cccttgtggt ttttcacatt ctttttcttc
     6001 ctgcaattct tccatctctt tatcctcctc ctcttcctct ttttcactgt cttcatcctc
     6061 ttcccttgtc aaactctctc tctcgtccga gtcgccctgt gagggagacg ccctggcacc
     6121 cacgtgctca ttcgagagga tttcaggccc tgcctcttcc tgctctgtgc tgtcacgttc
     6181 ttccgcttct ctcttacagt aggagtagcg atgattcatg tgttgagaat aagacccaga
     6241 gtgtgagaag cgctttccac atttgtcaca ttgatagggc ttttctccag aatgtaatcg
     6301 catgtgttca atcaaatgat gtttgtgttt aaatgccttt ttacagattc cacactcatg
     6361 aggtctttta cctgtgtgtt catatttatg tctcaataat gaactactct tttggaatat
     6421 cttgtcacac aaatcacaag catacattcc attttctgtc ttccgcattt tctttttggg
     6481 cggtgtagaa tcagagtcat tctgatcctc tacatttgat actccttctg agctagtatc
     6541 ttgtctttca tcctgatttc catttggctg gatcactttc aagggtggtt cttggactgc
     6601 agggctgacc gtagttgagt aggtgtatgc cacctgggga atcagaatcg tttgcttatt
     6661 ggcagctagc gctcttaagc atggaacact gttctggtca gcaatggcca cgattgtggg
     6721 taactgggca gtgactgtag gtatagcgat atttatgggg ttggcacttg gtgggattac
     6781 atttacaact ggttctgagt ctgtaacaca actgtccttt tgtggctcct tttttgcgca
     6841 agacaagttc aagggttctt cctggacaga ataaacactg ttctggtaaa cactagtgat
     6901 agttgacctt tctaataatt ctccctgttg ctttggtagt gaaagatcaa gaggttctac
     6961 ttgtggctct tcttgtgcac cctcagctgt gtacaagtaa ccctgtgtat ttctggatga
     7021 ggaaaggttt agaggtgatg gggatggtgt actacttctg gaaccattgg tggttgatcc
     7081 cactggtaaa actggggagt tagtcatctt caaaggactt tgtagattta ctgtgctgtc
     7141 ctggggttca tttgcatttg cagattgagg ctgatcattg ttcttggcag ggatatttac
     7201 tttgcctggt tcaggagaag atggttcaga agactgcact gaaatctgtc cagcttgcat
     7261 cttttcaaac cactttttta ctacatccag tggtaggttt actgaatcag caatttttga
     7321 gagctcttct gcacttggtt gtgcattcaa agcataatat gcttttagga gagacaagag
     7381 gttctttaaa ggtggctgac taggagacaa attgccatct ccagtagctg atgaaacaga
     7441 ggactcaggc ttctcagctt ctgctgcagg gagtggagga ggctgagtag gctgctttag
     7501 gtcatagtgc tttaattctg gaagtgcatt aatatctcct ggacaatcat cacacagaag
     7561 acaagtgcta tcattcaccc ccccttcaaa gcttttgtcc ttctcagact taacagtaag
     7621 atcttctggt aacttttcac ttttacaact gtttgtagcg actggatttt ctttttttaa
     7681 attttgagga acaacttgaa gttggctagg ctgctcaaga ctgtagttga tgataatttt
     7741 ggttgttcca tcttgatcaa ccaaaggaag actgatggct gaaataacag aatggccacc
     7801 ttgttgtatg ggtgaagcat tgattgtttc ttgttctttg gatgcaagat tggcttgatt
     7861 attctccaac acttgcctta ttacattacc atctaccgcc actttaagta cattctgaat
     7921 atcacttaaa ttgatactta tgggagacac caaaccaact gttggcagaa caacagcttg
     7981 caccatgccc tgaggagaac tggttgcctg taatgggcca ccaccagtga aaaccccatt
     8041 ttgtaaaggg gttgaacagt tgattcctga agcaaccact atgggtttga attcataatc
     8101 cacaggttca gttttaattt ggttaacaga aagttgttct tgaaggggtt tattctctat
     8161 cttttgccgt atctgtggtc gtgtgggact gcctggtgat gctgaaagag acggtgaaga
     8221 acactgagat gtcttgagtc ctgttcttgg tcgcccattc acaggtatca agctgataca
     8281 tttcttactg cttatgtgtg agctatagga gccagaatgg gaaaagcgtt tcttgcagtt
     8341 tgggcattca tatggcttct ctccactgtg aattcttaag tgctctttta ggtgatgttt
     8401 gtatttgaaa gcttttccac actcagtgca tttgaattta cgattacacc cagactgcgt
     8461 cacatgtctt tgatctcttc ctgatttatg tgatgtcatg tgacgttcaa gttgggttct
     8521 gtatgcaaag gtgtaactgc acagggagca actaaagtta tcttcattct tttcatgacg
     8581 atatttaatg tgttctttca gagaggtaaa gcgtttatag cctctatcac aatatggaca
     8641 ggtgagtaat tgtgaaaatg catctggtgt tccattttca tcatgaccac tggcttctgg
     8701 tgtgccctgc ctctggtcct cttcaggtgc ctcaggaaaa atgacagcag tgtcttgttg
     8761 ttgtagaaac tcttcaacat taggatcatg gttttgctca ttttctgcat ctgactcgca
     8821 ttcatcatct tttactgtac atcctgcttc atctgcctga gcttcaggcc ccaggatttc
     8881 ttgcccttcc tttcctgtgt catcctccca gcagttctta gcattccctt ctctttcact
     8941 gctcctccct ggtaacactg tctggtctgt tggcaggtca tcctctggta caccttcaca
     9001 gtcagctgca tctgtaacac tttcttcttc cacaatatgc agtttgtctt catcatctga
     9061 atctgaattt gtttctacca cagtattata atttgtaact ttcatcgaac cgcgggctag
     9121 cggatccgtc gacggtatcg ataagcttga ttcgaccggc cgactcagtc tgtcggagga
     9181 ctggcgcgcc gagtgagggg ttgtgagctc ttttatagag ctcgggaagc agaagcgcgc
     9241 gaacagaagc gagaagcagg ctgattggtt aattcaaata aggcgcaggg tcatttcagg
     9301 tccttggggg agcctggaaa catctgatgg gtcttaagaa actgctgagg gttgggccat
     9361 atctggggac catctgttct tggcctcggg ccggggccga aactgcggtg accatctgtt
     9421 cttggccccg ggccggggcc gaaactgctc accgcagata tcctgtttgg cccaacgtta
     9481 gctattttca tgtacccgcc cttgatctga acttctctat tcttggtttg gtatttttcc
     9541 atgccttgca aaatggcgtt actgcagcca gctcgaggct agtctcgtga tcgataaaat
     9601 tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt
     9661 ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta aaagttaacc
     9721 gataccgtcg agatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt
     9781 cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg
     9841 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
     9901 ttaaacctac caagcctcct actatcatta tgaataattt tatataccac agccaatttg
     9961 tt
//
