LOCUS       sequence-450454-       14783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB3 ZWF1 tet-on overexpression cassette with homologous flanks for 
            integration at pyrG locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14783)
  AUTHORS   Matthias Steiger
  TITLE     Regulation of the Glucose-6-phosphate dehydrogenase encoding gene 
            gsdA and its impact on growth and citric acid production in 
            Aspergillus niger
  JOURNAL   PLOS One
REFERENCE   2  (bases 1 to 14783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLOS One"
COMMENT     Sequence Label: pSF522
FEATURES             Location/Qualifiers
     source          1..14783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          619..734
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             join(752..1369,1370..1375,1376..1411,1412..1414,1415..1450,
                     1451..1453,1454..1489,1490..1498)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     protein_bind    join(1846..1864,1865..1887,1888..1906,1907..1929,1930..1948,
                     1949..1971,1972..1990,1991..2013,2014..2032,2033..2055,
                     2056..2074,2075..2097,2098..2116)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     intron          2186..2301
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     terminator      3840..4613
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene (Punt et al., 1987)"
     regulatory      9660..9669
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10253..11278
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             10253..11278
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(11941..11957)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11941..11957)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      12005..12036
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        12037..12139
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             12140..12955
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(12213..12232)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      12979..13006
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(13018..13605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13098..13117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13645..13662
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13646..13662
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggagcgcaaa aaaccccgcc cctgacaggg cggggttttt tcgcgatcgg agtcgataag
       61 cttcggagaa tatggagctt catcgaatca ccggcagtaa gcgaaggaga atgtgaagcc
      121 aggggtgtat agccgtcggc gaaatagcat gccattaacc taggtacaga agtccaattg
      181 cttccgatct ggtaaaagat tcacgagata gtaccttctc cgaagtaggt agagcgagta
      241 cccggcgcgt aagctcccta attggcccat ccggcatctg tagggcgtcc aaatatcgtg
      301 cctctcctgc tttgcccggt gtatgaaacc ggaaaggccg ctcaggagct ggccagcggc
      361 gcagaccggg aacacaagct ggcagtcgac ccatccggtg ctctgcactc gacctgctga
      421 ggtccctcag tccctggtag gcagctttgc cccgtctgtc cgcccggtgt gtcggcgggg
      481 ttgacaaggt cgttgcgtca gtccaacatt tgttgccata ttttcctgct ctccccacca
      541 gctgctcttt tcttttctct ttcttttccc atcttcagta tattcatctt cccatccaag
      601 aacctttatt tcccctaagt aagtactttg ctacatccat actccatcct tcccatccct
      661 tattcctttg aacctttcag ttcgagcttt cccacttcat cgcagcttga ctaacagcta
      721 ccccgcttga gcagacatca ccgaattcac catgtctaga ctggacaaga gcaaagtcat
      781 aaacggcgct ctggaattac tcaatggagt cggtatcgaa ggcctgacga caaggaaact
      841 cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc
      901 cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct
      961 ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcattcc gctgtgctct
     1021 cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta
     1081 cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc
     1141 actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga
     1201 gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct
     1261 gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct
     1321 ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccggccga
     1381 cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg actttgacct
     1441 tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaact
     1501 aagtaaggat ccactagtac agcagaagaa tctctctccg ctgttgcttc agtgtctgcc
     1561 atgcattaac ttcatcctac tgtcctaccc gcagtaccca ttcacatttg cggcagatac
     1621 caggttgttt tctattccct cggttcttca gttcttcaga tattacatta ctcgagaaat
     1681 tggcggcgat gcaggagttt ggttggtttt aattgtttca gtctccttga cctgatttgt
     1741 atgacatgca atgtttcgac ggatgactat ctcggtgaat atacgtcatg aatcatgcgg
     1801 ccgcgcgtat cacgaggccc tttcgacttc actcgagttt accactccct atcagtgata
     1861 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     1921 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     1981 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2041 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2101 ctatcagtga tagagaaaag tgaaagtcga gctccccatc ttcagtatat tcatcttccc
     2161 atccaagaac ctttatttcc cctaagtaag tactttgcta catccatact ccatccttcc
     2221 catcccttat tcctttgaac ctttcagttc gagctttccc acttcatcgc agcttgacta
     2281 acagctaccc cgcttgagca gacatcaccg tttaaacacc atgaccgata cgaaagccgt
     2341 agaatttgtg ggccacacag ccattgtagt ctttggagct tcaggggacc tggctaagaa
     2401 gaaaactttc cctgccctct tcggacttta ccgtgaggga tacctgtcca acaaggtgaa
     2461 gattattggc tatgctagat caaagctgga tgacaaggag ttcaaggata gaattgtggg
     2521 ctatttcaag acaaagaaca agggcgacga ggacaaagtt caagaattct taaagttgtg
     2581 ctcatatatt tcagctcctt atgacaaacc agatgggtat gaaaagttga atgaaactat
     2641 taacgaattc gaaaaggaaa acaacgtcga acagtctcac aggttgttct acttagcttt
     2701 gcccccttct gttttcatac ctgttgctac ggaggtcaag aagtatgttc atccaggttc
     2761 taaagggatt gctcggatta tcgtggaaaa acctttcggg cacgacttgc agtcagcaga
     2821 agagcttttg aatgctttga agccgatctg gaaagaagag gaattgttta gaatcgacca
     2881 ctatctaggt aaggagatgg ttaagaattt gttggccttc cgttttggaa acgcattcat
     2941 caatgcttct tgggacaaca gacatatcag ctgtatccaa atctcgttca aggagccttt
     3001 tggaacagaa ggtcgtggtg gctattttga ctcaattggt ataataagag acgtcattca
     3061 gaaccacttg cttcaagtgt taaccctctt aaccatggaa agacccgtct ctaatgaccc
     3121 tgaggctgtt agagatgaaa aggttcgcat tctgaagtca atttctgagc tagatttgaa
     3181 cgacgttttg gtgggtcaat acggcaaatc tgaggatgga aagaagccag cttatgtgga
     3241 tgatgaaact gttaagccag gttctaaatg tgtcacattt gcagccattg gcttgcacat
     3301 caacacagaa aggtgggaag gtgtcccaat cattttaaga gctggtaagg ctttgaacga
     3361 aggtaaagtt gagattagag tgcaatacaa acagtctact ggatttctca atgatattca
     3421 gcgaaatgaa ttggtcatcc gtgtgcagcc taacgaagcc atgtacatga aactgaactc
     3481 caaagtccca ggtgtttccc aaaagactac tgtcactgag ctagacctca cttacaaaga
     3541 ccgttacgaa aacttttaca ttccagaggc atatgaatca cttatcagag atgctatgaa
     3601 gggagatcac tctaattttg tcagagatga cgagttgata caaagttgga agattttcac
     3661 tcctttactg tatcacttgg agggccctga tgcaccggct ccagaaatct atccctacgg
     3721 atccagaggt ccagcttcat tgaccaaatt cttgcaagat catgattact tctttgaatc
     3781 acgcgacaat taccaatggc cagtgacaag acccgatgtg ctgcacaaga tgtaagcttg
     3841 atccacttaa cgttactgaa atcatcaaac agcttgacga atctggatat aagatcgttg
     3901 gtgtcgatgt cagctccgga gttgagacaa atggtgttca ggatctcgat aagatacgtt
     3961 catttgtcca agcagcaaag agtgccttct agtgatttaa tagctccatg tcaacaagaa
     4021 taaaacgcgt ttcgggttta cctcttccag atacagctca tctgcaatgc attaatgcat
     4081 tggacctcgc aaccctagta cgcccttcag gctccggcga agcagaagaa tagcttagca
     4141 gagtctattt tcattttcgg gagacgagat caagcagatc aacggtcgtc aagagtccta
     4201 cgagactgag gaatccgctc ttggctccac gcgactatat atttgtctct aattgtactt
     4261 tgacatgctc ctcttcttta ctctgatagc ttgactatga aaattccgtc accagcccct
     4321 gggttcgcaa agataattgc actgtttctt ccttgaactc tcaagcctac aggacacaca
     4381 ttcatcgtag gtataaacct cgaaaatcat tcctactaag atgggtatac aatagtaacc
     4441 atgcatggtt gcctagtgaa tgctccgtaa cacccaatac gccggccgaa acttttttac
     4501 aactctccta tgagtcgttt acccagaatg cacaggtaca cttgtttaga ggtaatcctt
     4561 ctttctagaa gtcctcgtgt actgtgtaag cgcccactcc acatctccac tcgctccggg
     4621 gagacgggca caaggttcat gaaccgaaga ggatgggaac cccgaccatg gcctgcggcc
     4681 gcagctatca gtcctctcag ctgacgcccc tcacgaaccc tcgcactctc ccgaaatgct
     4741 cttcggtgtc catggcgcat catctcagca gcatcccatg tcaaaccatg gctttgggcc
     4801 cacggactct gtggccctgc cgcaacatca ccatcaccat cgactcccgc cccatgcagc
     4861 gctgcgcgcc ccagggcgtc atggagtgaa tgtggaatct cctcctttgc cctcgggccc
     4921 gccgagtgtg gtgggccagc ctggaatgcc tgatccagcc cccaggcctc gaggaccaaa
     4981 actgaagttt actcccgaag aggacgctct actggtggag ttgaaggaaa acaagaactt
     5041 gacgtggaag caaattgcag acttcttccc gggccgaacg agcggtacct tgcaagtccg
     5101 atactgcacc aagctgaagg ctaaggatgt agcttggagt gacgaaatgg ttcgatttgc
     5161 tcctgatgta tttctacgcc tgtctcacac atgctaatga agggaatagg tacaaaggct
     5221 gcagcgggca atgcacgagt acgagaacga tcggtggcgc atcattgcag ggaaggttgg
     5281 aaatggcttc accccagctg cttgccgcga gaaagccatg cagctccatg agtaaaagcg
     5341 ttggggaatt ttcatattta tatctactgt cgccagattc ggccctgctt ggaccctctg
     5401 atctccttac tctccatatt ggttcaaatg tcgggtcacc gatagggctg gtggtgcagg
     5461 cttgttgtag gcacgggagg atgatcagca taactctgag tcactatagg gacgggttga
     5521 tgtaaggtat taagtgatgt atgataattc attttagccc gggggaacat atggcgccgg
     5581 catttgttcg ttcgcaatga accgacacta gcgtccgctc tcgcagttta gcaccggctg
     5641 atcccgggct gaacgcggcc attgctcggc cggggcatgt gttccttatc tacggcagac
     5701 cgcagatgac cactggagca gattatagac cctaagccct aagccggaca cccaatcgag
     5761 taggtctgcg gaccaggtca ctgcgggcag ccggagaagc tccgcaacca atcaatcccc
     5821 ggcgctgact aagggcaggc gaccacgggc cgaagcggct tcaaactcac ctcaacctcc
     5881 aaactccctc atctccaaac gtccttgcct tgtctgccgt cattgcaacc cacccaccag
     5941 gacacatgag cagcaagtcc cagctcacct acactgcccg tgccagcaag catcccaatg
     6001 ctctggcgaa gaggctgttc gagattgccg aggccaagaa aaccaatgtg actgtctcgg
     6061 ctgacgttac caccactaag gagctactag atcttgctga ccgtctcggt ccctacattg
     6121 ccgtgatcaa aacccacatc gatatcctct ctgatttcag caacgagact attgagggac
     6181 ttaaggctct cgcgcagaag cacaactttc tcatcttcga ggaccgcaag ttcattgaca
     6241 tcggcaacac ggtccagaag caataccacg gcggtaccct ccgtatctcg gaatgggccc
     6301 acatcatcaa ctgcagcatt ctccctggtg agggtatcgt cgaggctctc gctcagacgg
     6361 cgtctgcacc ggacttcgcc tacggccccg aacgcggtct gttgatcttg gcagagatga
     6421 cctctaaggg ctccttggct accggccagt acactacttc ctcggtcgat tatgcccgga
     6481 aatacaagaa cttcgttatg ggattcgtgt cgacgcgcgc gttgggtgag gtgcagtcgg
     6541 aagtcagctc tccttcggat gaggaggact ttgtggtttt cacgactggt gtgaacatta
     6601 acctccaagt cgcaattgac ctacgcttat gctgcagacg cgttacgtat cggatccaga
     6661 attcgtgata tctgaattcg tcgacaagct tattttttgt atactgtttt gtgatagcac
     6721 gaagtttttc cacggtatct tgttaaaaat atatatttgt ggcgggctta cctacatcaa
     6781 attaataaga gactaattat aaactaaaca cacaagcaag ctactttagg gtaaaagttt
     6841 ataaatgctt ttgacgtata aacgttgctt gtatttatta ttacaattaa aggtggatag
     6901 aaaacctaga gactagttag aaactaatct caggtttgcg ttaaactaaa tcagagcccg
     6961 agaggttaac agaacctaga aggggactag atatccgggt agggaaacaa aaaaaaaaaa
     7021 caagacagcc acatattagg gagactagtt agaagctagt tccaggacta ggaaaataaa
     7081 agacaatgat accacagtct agttgacaac tagatagatt ctagattgag gccaaagtct
     7141 ctgagatcca ggttagttgc aactaatact agttagtatc tagtctccta taactctgaa
     7201 gctagaataa cttactacta ttatcctcac cactgttcag ctgcgcaaac ggagtgattg
     7261 caaggtgttc agagactagt tattgactag tcagtgacta gcaataacta acaaggtatt
     7321 aacctaccat gtctgccatc accctgcact tcctcgggct cagcagcctt ttcctcctca
     7381 ttttcatgct cattttcctt gtttaagact gtgactagtc aaagactagt ccagaaccac
     7441 aaaggagaaa tgtcttacca ctttcttcat tgcttgtctc ttttgcatta tccatgtctg
     7501 caactagtta gagtctagtt agtgactagt ccgacgagga cttgcttgtc tccggattgt
     7561 tggaggaact ctccagggcc tcaagatcca caacagagcc ttctagatga ctggtcaata
     7621 actagttggt ctttgtctga gtctgactta cgaggttgca tactcgctcc ctttgcctcg
     7681 tcaatcgatg agaaaaagcg ccaaaactcg caatatggct ttgaaccaca cggtgctgag
     7741 actagttaga atctagtccc aaactagctt ggatagctta cctttgccct ttgcgttgcg
     7801 acaggtcttg cagggtatgg ttcctttctc accagctgat ttagctgcct tgctaccctc
     7861 acggcggatc tgcataaaga gtggctagag gttataaatt agcactgatc ctaggtacgg
     7921 ggctgaatgt aacttgcctt tcctttctca tcgcgcggca agacaggctt gctcaaattc
     7981 ctaccagtca caggggtatg cacggcgtac ggaccacttg aactagtcac agattagtta
     8041 gcaactagtc tgcattgaat ggctgtactt acgggccctc gccattgtcc tgatcatttc
     8101 cagcttcacc ctcgttgctg caaagtagtt agtgactagt caaggactag ttgaaatggg
     8161 agaagaaact cacgaattct cgacaccctt agtattgtgg tccttggact tggtgctgct
     8221 atatattagc taatacacta gttagactca cagaaactta cgcagctcgc ttgcgcttct
     8281 tggtaggagt cggggttggg agaacagtgc cttcaaacaa gccttcatac catgctactt
     8341 gactagtcag ggactagtca ccaagtaatc tagataggac ttgcctttgg cctccatcag
     8401 ttccttcata gtgggaggtc cattgtgcaa tgtaaactcc atgccgtggg agttcttgtc
     8461 cttcaagtgc ttgaccaata tgtttctgtt ggcagaggga acctgtcaac tagttaataa
     8521 ctagtcagaa actagtatag cagtagactc actgtacgct tgaggcatcc cttcactcgg
     8581 cagtagactt catatggatg gatatcaggc acgccattgt cgtcctgtgg actagtcagt
     8641 aactaggctt aaagctagtc gggtcggctt actatcttga aatccggcag cgtaagctcc
     8701 ccgtccttaa ctgcctcgag atagtgacag tactctgggg actttcggag atcgttatcg
     8761 cgaatgctcg gcatactaat cgttgactag tcttggacta gtcccgagca aaaaggattg
     8821 gaggaggagg aggaaggtga gagtgagaca aagagcgaaa taagagcttc aaaggctatc
     8881 tctaagcagt atgaaggtta agtatctagt tcttgactag atttaaaaga gatttcgact
     8941 agttatgtac ctggagtttg gatataggaa tgtgttgtgg taacgaaatg taagggggag
     9001 gaaagaaaaa gtcggtcaag aggtaactct aagtcggcca ttcctttttg ggaggcgcta
     9061 accataaacg gcatggtcga cttagagtta gctcagggaa tttagggagt tatctgcgac
     9121 caccgaggaa cggcggaatg ccaaagaatc ccgatggagc tctagctggc ggttgacaac
     9181 cccacctttt ggcgtttctg cggcgttgca ggcgggactg gatacttcgt agaaccagaa
     9241 aggcaaggca gaacgcgctc agcaagagtg ttggaagtga tagcatgatg tgccttgtta
     9301 actaggtcaa aatctgcagt atgcttgatg ttatccaaag tgtgagagag gaaggtccaa
     9361 acatacacga ttgggagagg gcctaggtat aagagttttt gagtagaacg catgtgagcc
     9421 cagccatctc gaggagatta aacacgggcc ggcatttgat ggctatgtta gtaccccaat
     9481 ggaaagcctg agagtccagt ggcgctaatt catgaataac ggtgagacta gcggccggtc
     9541 cccttatccc agctgttcca cgttggcctg cccctcagtt agcgctcaac tcaatgcccc
     9601 tcactggcga ggcgagggca aggatggagg ggcagcatcg cctgagttgg agcaaagcgg
     9661 ccgccatggg agcagcgaac caacggaggg atgccgtgct ttgtcgtggc tgctgtggcc
     9721 aatccgggcc cttggttggc tcacagagcg ttgctgtgag tccatgagct attattgcta
     9781 ggtacagtat agagagagga gagagagaga gagagagaga ggggaaaaaa ggtgaggttg
     9841 aagtgagaaa aaaaaaaaaa aaaatccaac cactgacggc tgccggctct gccacccccc
     9901 tccctccacc ccagaccacc tgcacactca gcgcgcagca tcacctaatc ttggctcgcc
     9961 ttcccgcagc tcaggttgtt ttttttttct ctctccctcg tcgaagccgc ccttgttccc
    10021 ttatttattt ccctctccat ccttgtctgc ctttggtcca tctgcccctt tgtctgcatc
    10081 tcttttgcac gcatcgcctt atcgtcgtct cttttttcac tcacgggagc ttgacgatga
    10141 cctgactcgt gagcctcacc tgctgatttc tctccccccc tcccgaccgg cttgactttt
    10201 gtttctcctc cagtacctta tcgcgaagcc ggaagaacct cttaacctct agatgaaaaa
    10261 gcctgaactc accgcgacgt ctgtcgagaa gttcctgatc gaaaagttcg acagcgtctc
    10321 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
    10381 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
    10441 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt
    10501 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
    10561 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc
    10621 tgcggccgat ctcagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
    10681 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
    10741 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
    10801 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
    10861 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
    10921 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
    10981 gcagcagacg cgctacttcg agcggaggca cccggagctt gcaggatcgc cgcggctccg
    11041 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
    11101 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
    11161 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
    11221 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagat
    11281 gcatggcttt cgtgaccggg cttcaaacaa tgatgtgcga tggtgtggtt cccggttggc
    11341 ggagtctttg tctactttgg ttgtctgtcg caggtcggta gaccgcaaat gagcaactga
    11401 tggattgttg ccagcgatac tataattcac atggatggtc tttgtcgatc agtagctagt
    11461 gagagagaga gaacatctat ccacaatgtc gagtgtctat tagacatact ccgagaataa
    11521 agtcaactgt gtctgtgatc taaagatcga ttcggcagtc gagtagcgta taacaactcc
    11581 gagtaccagc gaaagcacgt cgtgacagga gcagggcttt gccaactgcg caaccttgct
    11641 tgaatgagga tacacggggt gcaacatggc tgtactgatc catcgcaacc aaaatttctg
    11701 tttatagatc aagctggtag attccaatta ctccacctct tgcgcttctc catgacatgt
    11761 aagtgcacgt ggaaaccata cccaaattgc ctacagctgc ggagcatgag cctatggcga
    11821 tcagtctggt catgttaacc agcctgtgct ctgacgttaa tgcagaatag cttcgcgtga
    11881 tcttacggca ttatacgtat gatcggtcca cgatcagcta gattatctag tcagcttgat
    11941 gtcatagctg tttcctgagg ctcaatactg accatttaaa tcatacctga cctccatagc
    12001 agaaagtcaa aagcctccga ccggaggctt ttgacttgat cggcacgtaa gaggttccaa
    12061 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
    12121 aggagctaag gaagctaaaa tgagccatat tcaacgggaa acgtcttgct tgaagccgcg
    12181 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
    12241 gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct
    12301 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca ggctaaactg
    12361 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
    12421 atggttactc accactgcga tcccagggaa aacagcattc caggtattag aagaatatcc
    12481 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
    12541 tcctgtttgt aattgtcctt ttaacggcga tcgcgtattt cgtctcgctc aggcgcaatc
    12601 acgaatgaat aacggtttgg ttggtgcgag tgattttgat gacgagcgta atggctggcc
    12661 tgttgaacaa gtctggaaag aaatgcataa actcttgcca ttctcaccgg attcagtcgt
    12721 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
    12781 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
    12841 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
    12901 taatcctgat atgaataaat tgcagtttca cttgatgctc gatgagtttt tctaatgagg
    12961 acctaaatgt aatcacctgg ctcaccttcg ggtgggcctt tctgcgttgc tggcgttttt
    13021 ccataggctc cgcccccctg acgagcatca caaaaatcga tgctcaagtc agaggtggcg
    13081 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    13141 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    13201 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    13261 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    13321 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    13381 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    13441 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctc
    13501 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    13561 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatt
    13621 ttctaccgaa gaaaggccca cccgtgtaaa acgacggcca gtttatctag tcagcttgat
    13681 tctagctgat cgtggaccgg aaggtgagcc agtgagttga ttgcagtcca gttacgctgg
    13741 agtctgaggc tcgtcctgaa tgatatgcgg cccgctgtag gtcaattgcg acttggagga
    13801 agggatccgc caaggatgct ctggatccgc atccggccgc tcttgcgccc catcaatcgc
    13861 ccgactataa atcgaactac tttcggcatc ttctagactt cctaataccg cctagtcata
    13921 gcagattcaa gctgagaaca ccacaagtaa atatcaccca tcatgcttac cctgaccgtc
    13981 cctgaaaact acgggtatgt gccaattcta caattccttg cagacaatgc cattctcccc
    14041 atgaagtctg atgctaacta tcctgcagct ctgtcattgc cgtcgctctg ggtgccatcc
    14101 ccgtcctgag cttcgtccat ggcgccgtcg tgtctcgtct ccgcaaggaa gctgattgcc
    14161 cctaccctca ctgctatgcg accgtagagc agtgcaagac caacgtaagc caacctcaca
    14221 caaacaggat tcctcgagct aacatacatt ccgaaccgtg cagcccaagg ccgagcagtt
    14281 caactgcgct cagcgcgctc atgccaactt ccttgagaac tccagccaaa ctatgctctt
    14341 cctcctggta gctggactga agtaccccca gttggcgact ggcctcggaa gcatctgggt
    14401 cctcggtcgc tcactgttcc tttacggata tgtgtactcc ggcaagccgc ggggtcgcgg
    14461 tcgtttgtac ggcagcttct acttgcttgc acagggagct ctctggggct tgacgtcttt
    14521 tggagttgcg agggagttga tttcctactt ctaagtttgg actgaatccg tggtgtgatt
    14581 gaggtgattg gcgatgtttg gctataccag ctatatgtaa taatctctac tgtatactac
    14641 tattcaacgc attttactat gcgtgctgct agggtcggca atgacaatgg caatctgact
    14701 gacgtggtct atttctccat gtgcagcagg gaatacgagc tccaatggac ctcgggagtg
    14761 gcacagtcaa tggcaaggaa act
//
