LOCUS       sequence-450455-       15632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB3 gsdA tet-on overexpression cassette with homologous flanks for 
            integration at pyrG locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15632)
  AUTHORS   Matthias Steiger
  TITLE     Regulation of the Glucose-6-phosphate dehydrogenase encoding gene 
            gsdA and its impact on growth and citric acid production in 
            Aspergillus niger
  JOURNAL   PLOS One
REFERENCE   2  (bases 1 to 15632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLOS One"
COMMENT     Sequence Label: pSF503
FEATURES             Location/Qualifiers
     source          1..15632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          619..734
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             join(752..1369,1370..1375,1376..1411,1412..1414,1415..1450,
                     1451..1453,1454..1489,1490..1498)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     protein_bind    join(1846..1864,1865..1887,1888..1906,1907..1929,1930..1948,
                     1949..1971,1972..1990,1991..2013,2014..2032,2033..2055,
                     2056..2074,2075..2097,2098..2116)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     intron          2186..2301
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     terminator      4689..5462
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene (Punt et al., 1987)"
     regulatory      10509..10518
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11102..12127
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             11102..12127
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(12790..12806)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12790..12806)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      12854..12885
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        12886..12988
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             12989..13804
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(13062..13081)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      13828..13855
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(13867..14454)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13947..13966)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14494..14511
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14495..14511
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggagcgcaaa aaaccccgcc cctgacaggg cggggttttt tcgcgatcgg agtcgataag
       61 cttcggagaa tatggagctt catcgaatca ccggcagtaa gcgaaggaga atgtgaagcc
      121 aggggtgtat agccgtcggc gaaatagcat gccattaacc taggtacaga agtccaattg
      181 cttccgatct ggtaaaagat tcacgagata gtaccttctc cgaagtaggt agagcgagta
      241 cccggcgcgt aagctcccta attggcccat ccggcatctg tagggcgtcc aaatatcgtg
      301 cctctcctgc tttgcccggt gtatgaaacc ggaaaggccg ctcaggagct ggccagcggc
      361 gcagaccggg aacacaagct ggcagtcgac ccatccggtg ctctgcactc gacctgctga
      421 ggtccctcag tccctggtag gcagctttgc cccgtctgtc cgcccggtgt gtcggcgggg
      481 ttgacaaggt cgttgcgtca gtccaacatt tgttgccata ttttcctgct ctccccacca
      541 gctgctcttt tcttttctct ttcttttccc atcttcagta tattcatctt cccatccaag
      601 aacctttatt tcccctaagt aagtactttg ctacatccat actccatcct tcccatccct
      661 tattcctttg aacctttcag ttcgagcttt cccacttcat cgcagcttga ctaacagcta
      721 ccccgcttga gcagacatca ccgaattcac catgtctaga ctggacaaga gcaaagtcat
      781 aaacggcgct ctggaattac tcaatggagt cggtatcgaa ggcctgacga caaggaaact
      841 cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc
      901 cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct
      961 ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcattcc gctgtgctct
     1021 cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta
     1081 cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc
     1141 actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga
     1201 gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct
     1261 gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct
     1321 ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccggccga
     1381 cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg actttgacct
     1441 tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaact
     1501 aagtaaggat ccactagtac agcagaagaa tctctctccg ctgttgcttc agtgtctgcc
     1561 atgcattaac ttcatcctac tgtcctaccc gcagtaccca ttcacatttg cggcagatac
     1621 caggttgttt tctattccct cggttcttca gttcttcaga tattacatta ctcgagaaat
     1681 tggcggcgat gcaggagttt ggttggtttt aattgtttca gtctccttga cctgatttgt
     1741 atgacatgca atgtttcgac ggatgactat ctcggtgaat atacgtcatg aatcatgcgg
     1801 ccgcgcgtat cacgaggccc tttcgacttc actcgagttt accactccct atcagtgata
     1861 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     1921 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     1981 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2041 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2101 ctatcagtga tagagaaaag tgaaagtcga gctccccatc ttcagtatat tcatcttccc
     2161 atccaagaac ctttatttcc cctaagtaag tactttgcta catccatact ccatccttcc
     2221 catcccttat tcctttgaac ctttcagttc gagctttccc acttcatcgc agcttgacta
     2281 acagctaccc cgcttgagca gacatcaccg tttaaacacc atggccagca caatagcacg
     2341 cactgaggaa cgccagaatg ctgggtgagt tttgcggtct gcctctctga catcacaccc
     2401 ctcctcccac tcccgtccct cctgcccgcc gcccagacgt gaggattcac cacccacgac
     2461 tctccataac aagccccgtc gcccaaacat tcactggcag gcttcccgct ttccattatt
     2521 cttcaattcg tcaccaggat tactccttcg ggcttaacga aaggactatc cttctgactc
     2581 accaccaacc ctcactgccc ctcctgcatg ctgtagcgga ctgcgggcac cgacctgcat
     2641 catcgatcta cacccgatcc ctgtgacatt atattcgtca agctatagcc tagctaacat
     2701 ggatgtttta cgtagcacca tggagctcaa agatgacact gtcatcatag tactgggtgc
     2761 ctccggagat cttgcaaaga agaaaaccgt cagtgacgac cccctgattc atgttgacct
     2821 gacagaaagc taacctttta cagttcccgg cccttttcgg ccttgtatgt cctctcccag
     2881 atccaattgc agtttgactc accagtatgg ttgctgattt gcgcttccag tatcgcaaca
     2941 agttcctccc caagggaatc aagatcgtcg gatatgcccg gacaaacatg gaccatgagg
     3001 agtacctgag gcgtgtgcgc tcatacatca agacccctac caaggaaatc gaagagcagc
     3061 tggacagctt ctgccagttc tgcacctaca tttccggtca atatgacaag gatgactcgt
     3121 tcatcaacct caacaagcac ctcgaggaga ttgagaaggg ccagaaggag cagaacagaa
     3181 tctactacat ggccctccct cccagcgttt tcaccaccgt ttccgaccaa cttaagcgca
     3241 actgctaccc caagaacggc gttgcccgta tcatcgtgag tcaatcctgg gctggtatca
     3301 ccctgccatt ggtcattatt cttactcgct tgttttccta tttcacaggt agagaagcct
     3361 ttcggcaagg accttcagag ctcgcgcgat ctccaaaaag ccctggagcc taactggaag
     3421 gaagaggaga tcttccgtat cgaccactac ctgggtaagg agatggtcaa gaacatcctt
     3481 atcatgcgct tcggaaacga attcttcaac gccacctgga accgtcacca catcgataac
     3541 gttcaggtac gaccttgcgc tatccaattg gcctattgat ttacttgcta aattgtcgct
     3601 tctatcatta gatcacattc aaggagccct tcggcactga gggacgtggt ggttacttcg
     3661 atgaattcgg catcatccgt gatgtcatgc agaaccgtac gttcaaagtc acgctcgaca
     3721 tctccgacat gatgctgata aaaatctctc ctagaccttc tccaggtgtt gacgctgctc
     3781 gctatggagc gccccatttc cttctccgcc gaggacatcc gtgacgagaa ggtacagtgt
     3841 gcgcttgact attggttgtg ctgggttact gacacttaac caggttcgtg tcctccgtgc
     3901 gatggacgcc attgagccca agaacgtcat tattggccag tacggaaagt ctctggatgg
     3961 cagcaagccc gcctacaagg aggacgaaac cgttccccag gattcccgct gccccacctt
     4021 ctgcgctatg gtcgcctaca tcaagaacga gaggtgggac ggtgttcctt tcatcatgaa
     4081 ggctggcaag ggtatgtacc tctttccaag cgatcatagc accgattggt atactaataa
     4141 ttcgcagcct tgaacgagca gaaaaccgag atccgtatcc agttccgtga cgttacctcc
     4201 ggaattttca aggacatccc tcgcaacgag ctcgttatcc gcgtccagcc caacgagtcc
     4261 gtgtacatca agatgaactc caagctgcct ggcctgtcca tgcagacggt tgtgactgag
     4321 ctcgacctca cctaccgccg ccgcttctcc gacctcaaga tccccgaagc ctacgagtct
     4381 ctgatcctgg atgctctgaa gggcgaccac tccaacttcg tccgtgacga tgagctggat
     4441 gccagctgga ggatcttcac ccctctcctg cactacctgg atgacaacaa ggagatcatc
     4501 cccatggaat acccctacgg tacgtgcact tcttgcaatt tgtctaaatc gcttacatac
     4561 tgaccaacgc gcaggctccc gcggacccgc cgtccttgat gacttcaccg cgtccttcgg
     4621 ctacaagttc agcgatgctg ctggctacca gtggcccttg acttccaccc ccaaccgtct
     4681 gtaagcttga tccacttaac gttactgaaa tcatcaaaca gcttgacgaa tctggatata
     4741 agatcgttgg tgtcgatgtc agctccggag ttgagacaaa tggtgttcag gatctcgata
     4801 agatacgttc atttgtccaa gcagcaaaga gtgccttcta gtgatttaat agctccatgt
     4861 caacaagaat aaaacgcgtt tcgggtttac ctcttccaga tacagctcat ctgcaatgca
     4921 ttaatgcatt ggacctcgca accctagtac gcccttcagg ctccggcgaa gcagaagaat
     4981 agcttagcag agtctatttt cattttcggg agacgagatc aagcagatca acggtcgtca
     5041 agagtcctac gagactgagg aatccgctct tggctccacg cgactatata tttgtctcta
     5101 attgtacttt gacatgctcc tcttctttac tctgatagct tgactatgaa aattccgtca
     5161 ccagcccctg ggttcgcaaa gataattgca ctgtttcttc cttgaactct caagcctaca
     5221 ggacacacat tcatcgtagg tataaacctc gaaaatcatt cctactaaga tgggtataca
     5281 atagtaacca tgcatggttg cctagtgaat gctccgtaac acccaatacg ccggccgaaa
     5341 cttttttaca actctcctat gagtcgttta cccagaatgc acaggtacac ttgtttagag
     5401 gtaatccttc tttctagaag tcctcgtgta ctgtgtaagc gcccactcca catctccact
     5461 cgctccgggg agacgggcac aaggttcatg aaccgaagag gatgggaacc ccgaccatgg
     5521 cctgcggccg cagctatcag tcctctcagc tgacgcccct cacgaaccct cgcactctcc
     5581 cgaaatgctc ttcggtgtcc atggcgcatc atctcagcag catcccatgt caaaccatgg
     5641 ctttgggccc acggactctg tggccctgcc gcaacatcac catcaccatc gactcccgcc
     5701 ccatgcagcg ctgcgcgccc cagggcgtca tggagtgaat gtggaatctc ctcctttgcc
     5761 ctcgggcccg ccgagtgtgg tgggccagcc tggaatgcct gatccagccc ccaggcctcg
     5821 aggaccaaaa ctgaagttta ctcccgaaga ggacgctcta ctggtggagt tgaaggaaaa
     5881 caagaacttg acgtggaagc aaattgcaga cttcttcccg ggccgaacga gcggtacctt
     5941 gcaagtccga tactgcacca agctgaaggc taaggatgta gcttggagtg acgaaatggt
     6001 tcgatttgct cctgatgtat ttctacgcct gtctcacaca tgctaatgaa gggaataggt
     6061 acaaaggctg cagcgggcaa tgcacgagta cgagaacgat cggtggcgca tcattgcagg
     6121 gaaggttgga aatggcttca ccccagctgc ttgccgcgag aaagccatgc agctccatga
     6181 gtaaaagcgt tggggaattt tcatatttat atctactgtc gccagattcg gccctgcttg
     6241 gaccctctga tctccttact ctccatattg gttcaaatgt cgggtcaccg atagggctgg
     6301 tggtgcaggc ttgttgtagg cacgggagga tgatcagcat aactctgagt cactataggg
     6361 acgggttgat gtaaggtatt aagtgatgta tgataattca ttttagcccg ggggaacata
     6421 tggcgccggc atttgttcgt tcgcaatgaa ccgacactag cgtccgctct cgcagtttag
     6481 caccggctga tcccgggctg aacgcggcca ttgctcggcc ggggcatgtg ttccttatct
     6541 acggcagacc gcagatgacc actggagcag attatagacc ctaagcccta agccggacac
     6601 ccaatcgagt aggtctgcgg accaggtcac tgcgggcagc cggagaagct ccgcaaccaa
     6661 tcaatccccg gcgctgacta agggcaggcg accacgggcc gaagcggctt caaactcacc
     6721 tcaacctcca aactccctca tctccaaacg tccttgcctt gtctgccgtc attgcaaccc
     6781 acccaccagg acacatgagc agcaagtccc agctcaccta cactgcccgt gccagcaagc
     6841 atcccaatgc tctggcgaag aggctgttcg agattgccga ggccaagaaa accaatgtga
     6901 ctgtctcggc tgacgttacc accactaagg agctactaga tcttgctgac cgtctcggtc
     6961 cctacattgc cgtgatcaaa acccacatcg atatcctctc tgatttcagc aacgagacta
     7021 ttgagggact taaggctctc gcgcagaagc acaactttct catcttcgag gaccgcaagt
     7081 tcattgacat cggcaacacg gtccagaagc aataccacgg cggtaccctc cgtatctcgg
     7141 aatgggccca catcatcaac tgcagcattc tccctggtga gggtatcgtc gaggctctcg
     7201 ctcagacggc gtctgcaccg gacttcgcct acggccccga acgcggtctg ttgatcttgg
     7261 cagagatgac ctctaagggc tccttggcta ccggccagta cactacttcc tcggtcgatt
     7321 atgcccggaa atacaagaac ttcgttatgg gattcgtgtc gacgcgcgcg ttgggtgagg
     7381 tgcagtcgga agtcagctct ccttcggatg aggaggactt tgtggttttc acgactggtg
     7441 tgaacattaa cctccaagtc gcaattgacc tacgcttatg ctgcagacgc gttacgtatc
     7501 ggatccagaa ttcgtgatat ctgaattcgt cgacaagctt attttttgta tactgttttg
     7561 tgatagcacg aagtttttcc acggtatctt gttaaaaata tatatttgtg gcgggcttac
     7621 ctacatcaaa ttaataagag actaattata aactaaacac acaagcaagc tactttaggg
     7681 taaaagttta taaatgcttt tgacgtataa acgttgcttg tatttattat tacaattaaa
     7741 ggtggataga aaacctagag actagttaga aactaatctc aggtttgcgt taaactaaat
     7801 cagagcccga gaggttaaca gaacctagaa ggggactaga tatccgggta gggaaacaaa
     7861 aaaaaaaaac aagacagcca catattaggg agactagtta gaagctagtt ccaggactag
     7921 gaaaataaaa gacaatgata ccacagtcta gttgacaact agatagattc tagattgagg
     7981 ccaaagtctc tgagatccag gttagttgca actaatacta gttagtatct agtctcctat
     8041 aactctgaag ctagaataac ttactactat tatcctcacc actgttcagc tgcgcaaacg
     8101 gagtgattgc aaggtgttca gagactagtt attgactagt cagtgactag caataactaa
     8161 caaggtatta acctaccatg tctgccatca ccctgcactt cctcgggctc agcagccttt
     8221 tcctcctcat tttcatgctc attttccttg tttaagactg tgactagtca aagactagtc
     8281 cagaaccaca aaggagaaat gtcttaccac tttcttcatt gcttgtctct tttgcattat
     8341 ccatgtctgc aactagttag agtctagtta gtgactagtc cgacgaggac ttgcttgtct
     8401 ccggattgtt ggaggaactc tccagggcct caagatccac aacagagcct tctagatgac
     8461 tggtcaataa ctagttggtc tttgtctgag tctgacttac gaggttgcat actcgctccc
     8521 tttgcctcgt caatcgatga gaaaaagcgc caaaactcgc aatatggctt tgaaccacac
     8581 ggtgctgaga ctagttagaa tctagtccca aactagcttg gatagcttac ctttgccctt
     8641 tgcgttgcga caggtcttgc agggtatggt tcctttctca ccagctgatt tagctgcctt
     8701 gctaccctca cggcggatct gcataaagag tggctagagg ttataaatta gcactgatcc
     8761 taggtacggg gctgaatgta acttgccttt cctttctcat cgcgcggcaa gacaggcttg
     8821 ctcaaattcc taccagtcac aggggtatgc acggcgtacg gaccacttga actagtcaca
     8881 gattagttag caactagtct gcattgaatg gctgtactta cgggccctcg ccattgtcct
     8941 gatcatttcc agcttcaccc tcgttgctgc aaagtagtta gtgactagtc aaggactagt
     9001 tgaaatggga gaagaaactc acgaattctc gacaccctta gtattgtggt ccttggactt
     9061 ggtgctgcta tatattagct aatacactag ttagactcac agaaacttac gcagctcgct
     9121 tgcgcttctt ggtaggagtc ggggttggga gaacagtgcc ttcaaacaag ccttcatacc
     9181 atgctacttg actagtcagg gactagtcac caagtaatct agataggact tgcctttggc
     9241 ctccatcagt tccttcatag tgggaggtcc attgtgcaat gtaaactcca tgccgtggga
     9301 gttcttgtcc ttcaagtgct tgaccaatat gtttctgttg gcagagggaa cctgtcaact
     9361 agttaataac tagtcagaaa ctagtatagc agtagactca ctgtacgctt gaggcatccc
     9421 ttcactcggc agtagacttc atatggatgg atatcaggca cgccattgtc gtcctgtgga
     9481 ctagtcagta actaggctta aagctagtcg ggtcggctta ctatcttgaa atccggcagc
     9541 gtaagctccc cgtccttaac tgcctcgaga tagtgacagt actctgggga ctttcggaga
     9601 tcgttatcgc gaatgctcgg catactaatc gttgactagt cttggactag tcccgagcaa
     9661 aaaggattgg aggaggagga ggaaggtgag agtgagacaa agagcgaaat aagagcttca
     9721 aaggctatct ctaagcagta tgaaggttaa gtatctagtt cttgactaga tttaaaagag
     9781 atttcgacta gttatgtacc tggagtttgg atataggaat gtgttgtggt aacgaaatgt
     9841 aagggggagg aaagaaaaag tcggtcaaga ggtaactcta agtcggccat tcctttttgg
     9901 gaggcgctaa ccataaacgg catggtcgac ttagagttag ctcagggaat ttagggagtt
     9961 atctgcgacc accgaggaac ggcggaatgc caaagaatcc cgatggagct ctagctggcg
    10021 gttgacaacc ccaccttttg gcgtttctgc ggcgttgcag gcgggactgg atacttcgta
    10081 gaaccagaaa ggcaaggcag aacgcgctca gcaagagtgt tggaagtgat agcatgatgt
    10141 gccttgttaa ctaggtcaaa atctgcagta tgcttgatgt tatccaaagt gtgagagagg
    10201 aaggtccaaa catacacgat tgggagaggg cctaggtata agagtttttg agtagaacgc
    10261 atgtgagccc agccatctcg aggagattaa acacgggccg gcatttgatg gctatgttag
    10321 taccccaatg gaaagcctga gagtccagtg gcgctaattc atgaataacg gtgagactag
    10381 cggccggtcc ccttatccca gctgttccac gttggcctgc ccctcagtta gcgctcaact
    10441 caatgcccct cactggcgag gcgagggcaa ggatggaggg gcagcatcgc ctgagttgga
    10501 gcaaagcggc cgccatggga gcagcgaacc aacggaggga tgccgtgctt tgtcgtggct
    10561 gctgtggcca atccgggccc ttggttggct cacagagcgt tgctgtgagt ccatgagcta
    10621 ttattgctag gtacagtata gagagaggag agagagagag agagagagag gggaaaaaag
    10681 gtgaggttga agtgagaaaa aaaaaaaaaa aaatccaacc actgacggct gccggctctg
    10741 ccacccccct ccctccaccc cagaccacct gcacactcag cgcgcagcat cacctaatct
    10801 tggctcgcct tcccgcagct caggttgttt tttttttctc tctccctcgt cgaagccgcc
    10861 cttgttccct tatttatttc cctctccatc cttgtctgcc tttggtccat ctgccccttt
    10921 gtctgcatct cttttgcacg catcgcctta tcgtcgtctc ttttttcact cacgggagct
    10981 tgacgatgac ctgactcgtg agcctcacct gctgatttct ctccccccct cccgaccggc
    11041 ttgacttttg tttctcctcc agtaccttat cgcgaagccg gaagaacctc ttaacctcta
    11101 gatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag ttcctgatcg aaaagttcga
    11161 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
    11221 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
    11281 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
    11341 tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
    11401 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
    11461 tgcgatcgct gcggccgatc tcagccagac gagcgggttc ggcccattcg gaccgcaagg
    11521 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
    11581 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
    11641 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
    11701 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
    11761 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
    11821 ttgtatggag cagcagacgc gctacttcga gcggaggcac ccggagcttg caggatcgcc
    11881 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
    11941 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
    12001 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
    12061 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
    12121 ggaatagatg catggctttc gtgaccgggc ttcaaacaat gatgtgcgat ggtgtggttc
    12181 ccggttggcg gagtctttgt ctactttggt tgtctgtcgc aggtcggtag accgcaaatg
    12241 agcaactgat ggattgttgc cagcgatact ataattcaca tggatggtct ttgtcgatca
    12301 gtagctagtg agagagagag aacatctatc cacaatgtcg agtgtctatt agacatactc
    12361 cgagaataaa gtcaactgtg tctgtgatct aaagatcgat tcggcagtcg agtagcgtat
    12421 aacaactccg agtaccagcg aaagcacgtc gtgacaggag cagggctttg ccaactgcgc
    12481 aaccttgctt gaatgaggat acacggggtg caacatggct gtactgatcc atcgcaacca
    12541 aaatttctgt ttatagatca agctggtaga ttccaattac tccacctctt gcgcttctcc
    12601 atgacatgta agtgcacgtg gaaaccatac ccaaattgcc tacagctgcg gagcatgagc
    12661 ctatggcgat cagtctggtc atgttaacca gcctgtgctc tgacgttaat gcagaatagc
    12721 ttcgcgtgat cttacggcat tatacgtatg atcggtccac gatcagctag attatctagt
    12781 cagcttgatg tcatagctgt ttcctgaggc tcaatactga ccatttaaat catacctgac
    12841 ctccatagca gaaagtcaaa agcctccgac cggaggcttt tgacttgatc ggcacgtaag
    12901 aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc
    12961 gagattttca ggagctaagg aagctaaaat gagccatatt caacgggaaa cgtcttgctt
    13021 gaagccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
    13081 taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga
    13141 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
    13201 gctaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
    13261 tgatgatgca tggttactca ccactgcgat cccagggaaa acagcattcc aggtattaga
    13321 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
    13381 gcattcgatt cctgtttgta attgtccttt taacggcgat cgcgtatttc gtctcgctca
    13441 ggcgcaatca cgaatgaata acggtttggt tggtgcgagt gattttgatg acgagcgtaa
    13501 tggctggcct gttgaacaag tctggaaaga aatgcataaa ctcttgccat tctcaccgga
    13561 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
    13621 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
    13681 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
    13741 tggtattgat aatcctgata tgaataaatt gcagtttcac ttgatgctcg atgagttttt
    13801 ctaatgagga cctaaatgta atcacctggc tcaccttcgg gtgggccttt ctgcgttgct
    13861 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgat gctcaagtca
    13921 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    13981 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    14041 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    14101 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    14161 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    14221 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    14281 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    14341 agttacctcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    14401 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    14461 cctttgattt tctaccgaag aaaggcccac ccgtgtaaaa cgacggccag tttatctagt
    14521 cagcttgatt ctagctgatc gtggaccgga aggtgagcca gtgagttgat tgcagtccag
    14581 ttacgctgga gtctgaggct cgtcctgaat gatatgcggc ccgctgtagg tcaattgcga
    14641 cttggaggaa gggatccgcc aaggatgctc tggatccgca tccggccgct cttgcgcccc
    14701 atcaatcgcc cgactataaa tcgaactact ttcggcatct tctagacttc ctaataccgc
    14761 ctagtcatag cagattcaag ctgagaacac cacaagtaaa tatcacccat catgcttacc
    14821 ctgaccgtcc ctgaaaacta cgggtatgtg ccaattctac aattccttgc agacaatgcc
    14881 attctcccca tgaagtctga tgctaactat cctgcagctc tgtcattgcc gtcgctctgg
    14941 gtgccatccc cgtcctgagc ttcgtccatg gcgccgtcgt gtctcgtctc cgcaaggaag
    15001 ctgattgccc ctaccctcac tgctatgcga ccgtagagca gtgcaagacc aacgtaagcc
    15061 aacctcacac aaacaggatt cctcgagcta acatacattc cgaaccgtgc agcccaaggc
    15121 cgagcagttc aactgcgctc agcgcgctca tgccaacttc cttgagaact ccagccaaac
    15181 tatgctcttc ctcctggtag ctggactgaa gtacccccag ttggcgactg gcctcggaag
    15241 catctgggtc ctcggtcgct cactgttcct ttacggatat gtgtactccg gcaagccgcg
    15301 gggtcgcggt cgtttgtacg gcagcttcta cttgcttgca cagggagctc tctggggctt
    15361 gacgtctttt ggagttgcga gggagttgat ttcctacttc taagtttgga ctgaatccgt
    15421 ggtgtgattg aggtgattgg cgatgtttgg ctataccagc tatatgtaat aatctctact
    15481 gtatactact attcaacgca ttttactatg cgtgctgcta gggtcggcaa tgacaatggc
    15541 aatctgactg acgtggtcta tttctccatg tgcagcaggg aatacgagct ccaatggacc
    15601 tcgggagtgg cacagtcaat ggcaaggaaa ct
//
