LOCUS       sequence-450547-        4126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' homology arm for S. pombe genomic integration. Part type 8b 
            following the YeastToolkit MoClo grammar..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Hebra T, Smrckova H, Elkatmis B, Prevorovsky M, Pluskal T
  TITLE     POMBOX: A Fission Yeast Cloning Toolkit for Molecular and Synthetic 
            Biology.
  JOURNAL   ACS Synth Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529.
  PUBMED    37991801
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529."
COMMENT     Sequence Label: pPOM038_P8b_5'Lys3
FEATURES             Location/Qualifiers
     source          1..4126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(57..73)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(57..73)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(70..92)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(245..833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(325..344)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(950..974)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(1074..1865,1866..1934))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1697..1716
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1935..2039)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2138..2160
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2152..2169
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2153..2169
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca
       61 tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat
      121 ccgacaatct ccaagacatt aggtggagtt cagttcggcg tatggcatat gtcgctggaa
      181 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      241 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      301 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      361 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      421 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      481 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      541 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      601 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      661 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      721 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      781 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      841 ctttgatctt ttctacgggg tctgacgctc tattcaacaa agccgccgtc ccgtcaagtc
      901 agcgtaaatg ggtagggggc ttcaaatcgt cctcgtgata ccaattcgga gcctgctttt
      961 ttgtacaaac ttgttgataa tggcaattca aggatcttca cctagatcct tttaaattaa
     1021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1201 gcaatgatac cgcgagagcc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     1561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     1741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     1801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     1861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     1921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     1981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2041 acatttcccc gaaaagtgcc agatacctga aacaaaaccc atcgtacggc caaggaagtc
     2101 tccaataact gtgatccacc acaagcgcca gggttttccc agtcacgacg ttgtaaaacg
     2161 acggccagtc atgcataatc cgcacgcatc tggaataagg aagtgccatt ccgcctgacc
     2221 tactagacta gacaaccgtc tcatcggtct cacaatggga tgccaaagtt agatcctact
     2281 agtaaaaatc aagtgaattg tgcctaggat aggtagctaa cgtgactgcc actgaacctg
     2341 tgtagcgtat tagatccatc aagatccctt ctgctcatat cattgctgat ctgtaaattg
     2401 accgcatcaa atttgatctc atttatatcg agattgatct atattatttt gccaatttgc
     2461 agtatataaa cttatttgat taagtttttg ctctccttcg ccacgtcttc attttgttag
     2521 tttaaatttt tttgactaac ttagaaaggg aagtttttaa aaacgttgaa atactttttc
     2581 gatttaaaaa tatatattta attaattttc tgttccaaaa tggttgctcc tcatctttgg
     2641 ttacgtgctg aaaccaaacc tttagaagag cgcagtgctt tgactcctag aactgccaag
     2701 atactcgccg atgctggatt tcaaattacc attgagcgct cttcccaacg tgctttcaag
     2761 gacaaagaat ttgagcgcct tggtttcccc atggttcctg agggctcatg gcgacatgca
     2821 cctaaggacg cgtacattat tggactcaaa gagcttccgg aaaatgataa cagtcctttg
     2881 aagcatactc atattcaatt tgctcattgc tacaaaaacc aggaaggttg gcgtgaagtc
     2941 ttgtcccgat tccccgctgg caatggtctt ctatatgatt tggaattttt gcaagatgac
     3001 aatggcagac gtgttgccgc ttttggttac cacgctggtt ttgctggatc agctatctca
     3061 tgtttagttt gggcacatca attattgcat cctaacaaac aattccctgc tatccgtccg
     3121 ttccccaacg aaaaatcttt ggttcgccat gttgctcgtc aagtacgttt agctttgaaa
     3181 aagaataaca accagtatcc tcgtattctg gttattgggg ctcttggtcg ttgtggtact
     3241 ggagcgtgcg atttagcatc aaagattggt attcccttcg ataacattct tcgctgggac
     3301 attaatgaaa ctaagaaggg tggtcccttt accgaaatta cggaatcaga catatttgtt
     3361 aactgcattt atctgagcat gcctattccc aaattctgta ccgtcgagtc gttaaatgta
     3421 cctaaccgta aattgcgtgt tgtttgtgat gttagctgtg acactaccaa tcctaacaat
     3481 cctattccaa tttataatgt aaacactaca tttgatcatc ctacagttga ggttaaaggt
     3541 gtcacaactc ctcctccttt agaggtcatt tctattgatc atcttcctac tttattgcca
     3601 agggaatctt ccgaagcatt tagtgaagct ctaattccta gtcttttggc tttgaaggac
     3661 gtcgacaatg ctcccgtttg ggtcagagct aaaaaacttt atgaaaccat ggttcaaaag
     3721 ctttaaaata aatgttattt aatttcaaag ttccgggtcc tttatattgt ttcaccaaat
     3781 tattgaatat tattttctca aaaattaact ttagaataca atttagacgg cattttcgat
     3841 gctctggaca ttttttccac gtacttgtta aattgtagcg gtgttgcata atactacctt
     3901 ttgatttgtt attcacgtta caatatatat ttgactaatt taaatatgat aatatacaca
     3961 tttctctgca aacctccttt atacttttcg ccggtcattg attaataatt aagaaacagg
     4021 atttcatcct gcatttgcca cttacctata tggtaaatat aaatcttgaa actttcccca
     4081 atatttattg cttcccttga gacctgagac ggttgtgttg tgttgt
//
