LOCUS       sequence-45057-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Liao JC, Lam R, Brazda V, Duan S, Ravichandran M, Ma J, Xiao T, 
            Tempel W, Zuo X, Wang YX, Chirgadze NY, Arrowsmith CH
  TITLE     Interferon-inducible protein 16: insight into the interaction with 
            tumor suppressor p53.
  JOURNAL   Structure. 2011 Mar 9;19(3):418-29.
  PUBMED    21397192
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Structure."; date: "2011-03-9"; volume: "19(3)"; pages: "418-29"
COMMENT     Sequence Label: IFI16-(192-393)-HinA-E222A-E272A-E381A
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(753..771)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      767..813
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attttgttta ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgcaggtaa ctcccagaag aaatgttctc
      121 caaaaacgcc cagtgatagt gaaggtactg agtacaacaa agccatttga atatgagacc
      181 ccagcaatgg agaaaaaaat aatgtttcat gctacagtgg ctacacagac acagttcttc
      241 catgtgaagg ttttaaacac cagcttgaag gagaaattca atggaaagaa aatcatcatc
      301 atatcagatt atttggaata tgatagtctc ctagcggtca atgaagaatc tactgtatct
      361 gaagctggtc ctaaccaaac gtttgaggtt ccaaataaaa tcatcaacag agcaaaggaa
      421 actctgaaga ttgatattct tcacaaacaa gcttcaggaa atattgtata tggggtattt
      481 atgctacata agaaaacagt aaatcagaag accacaatct acgaaattca ggatgataga
      541 ggaaaaatgg atgtagtggg gacaggacaa tgtcacaata tcccctgtga agaaggagat
      601 aagctccaac ttttctgctt tcgacttaga aaaaagaacc agatgtcaaa actgatttca
      661 gcaatgcata gttttatcca gataaagaaa aaaacaaact agggatccgg ctgctaacaa
      721 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      781 tggggcctct aaacgggtct tgaggggttt ttttgctgaa aggaggaact atattccgga
      841 tatcccgcaa gaggcccggc agtaccggca ctaacccagc ctatgcccta cagcatcc
//
