LOCUS       sequence-45058-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Tsai BP, Wang X, Huang L, Waterman ML
  TITLE     Quantitative profiling of in vivo-assembled RNA-protein complexes 
            using a novel integrated proteomic approach.
  JOURNAL   Mol Cell Proteomics. 2011 Apr;10(4):M110.007385. Epub 2011 Feb 1.
  PUBMED    21285413
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2011-04"; volume: "10(4)"; pages: "M110.007385.
            Epub 2011 Feb 1"
COMMENT     Sequence Label: MS2-HB
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             104..490
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGLY"
     CDS             104..490
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGLY"
     CDS             887..904
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gctgttttga cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt
       61 gcattggaac ggacctgcag gaattgatcc gcggccgcgc atggcttcta actttactca
      121 gttcgttctc gtcgacaatg gcggaactgg cgacgtgact gtcgccccaa gcaacttcgc
      181 taacggggtc gctgaatgga tcagctctaa ctcgcgttca caggcttaca aagtaacctg
      241 tagcgttcgt cagagctctg cgcagaagcg caaatacacc atcaaagtcg aggtgcctaa
      301 agtggcaacc cagactgttg gtggtgtaga gcttcctgta gccgcatggc gttcgtactt
      361 aaatatggaa ctaaccattc caattttcgc tacgaattcc gactgcgagc ttattgttaa
      421 ggcaatgcaa ggtctcctaa aagatggaaa cccgattccc tcagcaatcg cagcaaactc
      481 cggcctctac ggcgcaatgg cttctaactt tactcagttc gttctcgtcg acaatggcgg
      541 aactggcgac gtgactgtcg ccccaagcaa cttcgctaac ggggtcgctg aatggatcag
      601 ctctaactcg cgttcacagg cttacaaagt aacctgtagc gttcgtcaga gctctgcgca
      661 gaagcgcaaa tacaccatca aagtcgaggt acctaaagtg gcaacccaga ctgttggtgg
      721 tgtagagctt cctgtagccg catggcgttc gtacttaaat atggaactaa ccattccaat
      781 tttcgccacg aattccgact gcgagcttat tgttaaggca atgcaaggtc tcctaaaaga
      841 tggaaacccg attccctcag caatcgcagc aaactcctta attaaccatc atcaccacca
      901 tcatga
//
