LOCUS       sequence-45064-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Breckenridge MT, Dulyaninova NG, Egelhoff TT
  TITLE     Multiple regulatory steps control mammalian nonmuscle myosin II 
            assembly in live cells.
  JOURNAL   Mol Biol Cell. 2009 Jan;20(1):338-47. Epub 2008 Oct 29.
  PUBMED    18971378
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2009-01"; volume: "20(1)"; pages: "338-47. Epub 2008 
            Oct 29"
COMMENT     Sequence Label: pCMV-eGFP-NMHC-IIA-Delta-tailpiece
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..29
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     269..290
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      332..341
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(380..583)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(409..429)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
       61 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
      121 aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
      181 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      241 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
      301 accccggtga acagctcctc gcccttgctc accatggtgg cgaccggtag cgctagcgga
      361 tctgacggtt cactaaacca gctctgctta tatagacctc ccaccgtaca cgcctaccgc
      421 ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt
      481 gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa
      541 accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga
      601 ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat
      661 gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca
      721 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga
      781 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat
//
