LOCUS       sequence-450657-        8141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter vector containing mouse Zc3h12a 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Bataclan M, Leoni C, Moro SG, Pecoraro M, Wong EH, Heissmeyer V, 
            Monticelli S
  TITLE     Crosstalk between Regnase-1 and -3 shapes mast cell survival and 
            cytokine expression.
  JOURNAL   Life Sci Alliance. 2024 Jun 3;7(8):e202402784. doi: 
            10.26508/lsa.202402784. Print 2024 Aug.
  PUBMED    38830770
REFERENCE   2  (bases 1 to 8141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2024-06-3"; volume: "7(8):e202402784. doi"; 
            pages: " 10.26508/lsa.202402784. Print 2024 Aug"
COMMENT     Sequence Label: pmiRGLO-Zc3h12a 3'UTR
FEATURES             Location/Qualifiers
     source          1..8141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    732..853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(743..762)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     797..816
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1098..1455
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1098..1289
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1121..1141)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1306..1441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      1500..1509
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1506..2438
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             2490..3281
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2541..2560)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3151..3170
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3345..3393
                     /note="synthetic polyadenylation signal"
     promoter        3549..3653
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3654..3722,3723..4514)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3872..3891)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4672..5260)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4752..4771)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    5376..5659
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        5716..6215
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5751..5774)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6148..6167
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      6256..6265
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6262..7914
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 gtgatcaccc tgttgataca cattgtatct ctgtcattta aggagacgct gccggtcagg
       61 ccgtccatcc gtggctgatg cccaaaccct cttttttttt tctcagaggg ccgggtggga
      121 ggcatggggg agcagaggcc tgagctggac cccaccttcc accctgtccc tgggacgccg
      181 gcaccaccgg ctagttaggc accatgtgcc tgctctctga ggccccctca agccaatgcg
      241 gcctcatccc tgttcacagg gcatgaggct tcatgttagt aagcaagatg cttctttaag
      301 cccctcccct gcccgctctg tccacctaca cacccccccc ccaaccaggg ctccaaggcc
      361 ctctgtttcc acacctccca tgggtgggag gacacatgta tgctgtgtac agaggcgaga
      421 tttaaatatt ttaaatgaaa aaggttgaca aaataaaggc tattgccagg caggctggag
      481 agatggctca gtggttaaga gcaccgactg ctcttctgaa ggtcctgagt tcaaatgtca
      541 gcaaccacat ggtggctcac aaacatctgt gatgagatct ggtgccctct tctgggtgtc
      601 tgaagtcgac ctgcaggcat gcaagctgat ccggctgcta acaaagcccg aaaggaagct
      661 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ggccgcttcg
      721 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
      781 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
      841 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat
      901 gtgggaggtt tttttaagca agtaaaacct ctacaaatgt ggtaaaatcg aattttaaca
      961 aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     1021 ttcacaccgc atacgcggat ctgcgcagca ccatggcctg aaataacctc tgaaagagga
     1081 acttggttag gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     1141 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     1201 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     1261 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     1321 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     1381 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     1441 ctaggctttt gcaaaaagct tgattcttct gacacaacag tctcgaacca aaggctggag
     1501 ccaccatggc ttccaaggtg tacgaccccg agcaacgcaa acgcatgatc actgggcctc
     1561 agtggtgggc tcgctgcaag caaatgaacg tgctggactc cttcatcaac tactatgatt
     1621 ccgagaagca cgccgagaac gccgtgattt ttctgcatgg taacgctgcc tccagctacc
     1681 tgtggaggca cgtcgtgcct cacatcgagc ccgtggctag atgcatcatc cctgatctga
     1741 tcggaatggg taagtccggc aagagcggga atggctcata tcgcctcctg gatcactaca
     1801 agtacctcac cgcttggttc gagctgctga accttccaaa gaaaatcatc tttgtgggcc
     1861 acgactgggg ggcttgtctg gcctttcact actcctacga gcaccaagac aagatcaagg
     1921 ccatcgtcca tgctgagagt gtcgtggacg tgatcgagtc ctgggacgag tggcctgaca
     1981 tcgaggagga tatcgccctg atcaagagcg aagagggcga gaaaatggtg cttgagaata
     2041 acttcttcgt cgagaccatg ctcccaagca agatcatgcg gaaactggag cctgaggagt
     2101 tcgctgccta cctggagcca ttcaaggaga agggcgaggt tagacggcct accctctcct
     2161 ggcctcgcga gatccctctc gttaagggag gcaagcccga cgtcgtccag attgtccgca
     2221 actacaacgc ctaccttcgg gccagcgacg atctgcctaa gatgttcatc gagtccgacc
     2281 ctgggttctt ttccaacgct attgtcgagg gagctaagaa gttccctaac accgagttcg
     2341 tgaaggtgaa gggcctccac ttcagccagg aggacgctcc agatgaaatg ggtaagtaca
     2401 tcaagagctt cgtggagcgc gtgctgaaga acgagcagac cggtggtggg agcggaggtg
     2461 gcggatcagg tggcggaggc tccggaggga ttgaacaaga tggattgcac gcaggttctc
     2521 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     2581 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     2641 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     2701 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     2761 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     2821 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     2881 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     2941 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     3001 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     3061 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     3121 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     3181 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     3241 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     3301 aatgaccgac caagcgacgc ccaacctgcc atcacgatgg ccgcaataaa atatctttat
     3361 tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agcgataagg atcctctttg
     3421 cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc
     3481 gtttctgcgg actggctttc tacgtaatgg tttcttagac gtcaggtggc acttttcggg
     3541 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3601 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     3661 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3721 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     3781 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     3841 gttttccaat gatgagcact ttcaaagttc tgctatgtgg cgcggtatta tcccgtattg
     3901 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     3961 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4021 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaact atcggaggac
     4081 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4141 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4201 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4261 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4321 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4381 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4441 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4501 ttaagcattg gtaattcgaa atgaccgacc aagcgacgcc caaccggtat cagctcactc
     4561 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     4621 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4681 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4741 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4801 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4861 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4921 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4981 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5041 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5101 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5161 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5221 ttgcaagcag cagattacgc gcagaaaaaa aggatttcaa gaagatcctt tgatcttttc
     5281 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5341 atcaaaaagg atcttcacct agatcctttt atagtccgga aatacaggaa cgcacgctgg
     5401 atggcccttc gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg attaagtggg
     5461 ggtaatgtgg cctgtaccct ctggttgcat aggtattcat acggttaaaa tttatcaggc
     5521 gcgattgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     5581 gcgctgaacg cgttttagcg gtgcgtacaa ttaagggatt atggtaaatc cacttactgt
     5641 ctgccctcgt agccatcgag ataaaccgca gtactccggc cacgatgcgt ccggcgtaga
     5701 ggatcgagat ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     5761 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     5821 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     5881 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     5941 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     6001 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     6061 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     6121 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     6181 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttggcaatcc
     6241 ggtactgttg gtaaagccac catggaagat gccaaaaaca ttaagaaggg cccagcgcca
     6301 ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat gaagcgctac
     6361 gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac
     6421 gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat
     6481 acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat gcccgtgttg
     6541 ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa cgagcgcgag
     6601 ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa gaaagggctg
     6661 caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat catcatggat
     6721 agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc ccatttgcca
     6781 cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa aaccatcgcc
     6841 ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct accgcaccgc
     6901 accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca gatcatcccc
     6961 gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt caccacgctg
     7021 ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc
     7081 ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac actatttagc
     7141 ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca cgagatcgcc
     7201 agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg cttccaccta
     7261 ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct gatcaccccc
     7321 gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg
     7381 gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt
     7441 ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc tctcatcgac
     7501 aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc
     7561 atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc cccagccgaa
     7621 ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc cggcctgccc
     7681 gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg
     7741 accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa gaagctgcgc
     7801 ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc
     7861 aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt gtaattctag
     7921 ttgtttaaac gagctcgcta gcgaattccc cgggctcgag gccagaaggt ggcgcaaggg
     7981 gctgtcgggc atcacagata gcggtcccca gcccctgcct ggcctaccct ggaagctgga
     8041 cagaaggaca agtgaagcag gcaagggaat cctcaaacca aagataccat aggattggtt
     8101 ctggccccgc ggcaccccaa cccgtcccgc acaggtcaga a
//
