LOCUS       sequence-45067-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Mark WY, Liao JC, Lu Y, Ayed A, Laister R, Szymczyna B, Chakrabartty
            A, Arrowsmith CH
  TITLE     Characterization of segments from the central region of BRCA1: an 
            intrinsically disordered scaffold for multiple protein-protein and 
            protein-DNA interactions?
  JOURNAL   J Mol Biol. 2005 Jan 14;345(2):275-87.
  PUBMED    15571721
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-01-14"; volume: "345(2)"; pages: "275-87"
COMMENT     Sequence Label: BRCA1-(170-360)
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(723..741)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      737..784
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggtgcc gcgcggcagc catatgcgga tacaacctca aaagacgtct
      121 gtctacattg aattgggatc tgattcttct gaagataccg ttaataaggc aacttattgc
      181 agtgtgggag atcaagaatt gttacaaatc acccctcaag gaaccaggga tgaaatcagt
      241 ttggattctg caaaaaaggc tgcttgtgaa ttttctgaga cggatgtaac aaatactgaa
      301 catcatcaac ccagtaataa tgatttgaac accactgaga agcgtgcagc tgagaggcat
      361 ccagaaaagt atcagggtag ttctgtttca aacttgcatg tggagccatg tggcacaaat
      421 actcatgcca gctcattaca gcatgagaac agcagtttat tactcactaa agacagaatg
      481 aatgtagaaa aggctgaatt ctgtaataaa agcaaacagc ctggcttagc aaggagccaa
      541 cataacagat gggctggaag taaggaaaca tgtaatgata ggcggactcc cagcacagaa
      601 aaaaaggtag atctgaatgc tgatcccctg tgtgagagaa aagaatggaa taagcagaaa
      661 ctgccatgct aaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
      721 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      781 tttgctgaaa ggaggaacta tatccggata tcccgcaaga ggcccggcag taccggcata
      841 accaagccta tgcctacagc atccagggtg acggtgccga ggatgaccat gagcgcattg
      901 ttagattc
//
