LOCUS       sequence-450682-        9142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor BACH2 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9142)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 9142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-BACH2-Brd 182
FEATURES             Location/Qualifiers
     source          1..9142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1809..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1960..2366
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    5146..5549
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             5668..5901
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5973..6094
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6010..6029)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6064..6083
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6134..6269
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6289..6308)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6290..6308)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6318..6335)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6318..6334)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6327..6349)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6476..6931
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6563..6582)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6773..6794
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6957..7061
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7062..7130,7131..7922)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7280..7299)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8093..8681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8582..8601
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8835..8852
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8969..8990
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9005..9010,9011..9028,9029..9035)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9043..9059
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9048..9070
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9067..9083
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9067..9083
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9102..9122
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9104..9122
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1921 aattttatcg atcacgagac tagcctcgag ctggctgcag taacgccatt ttgcaaggca
     1981 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2041 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2101 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2161 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2221 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2281 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2341 gtcctccgac agactgagtc ggccggtcga atcaagctta tcgataccgt cgacgccacc
     2401 atgtccgtgg acgagaagcc tgacagccct atgtacgtgt acgagagcac cgtgcactgc
     2461 accaacatcc tgctgggcct gaacgaccag cggaagaaag acatcctgtg cgacgtgacc
     2521 ctgatcgtgg aacggaaaga gttcagagcc cacagagccg tgctggccgc ctgtagcgag
     2581 tatttttggc aggccctcgt gggccagacc aagaacgatc tggtggtgtc cctgcctgag
     2641 gaagtgaccg ccagaggatt tggacccctg ctgcagtttg cctacaccgc caaactgctg
     2701 ctgagccgcg agaacatccg ggaagtgatc agatgcgccg agttcctgcg gatgcacaac
     2761 ctggaagata gctgcttcag cttcctgcag acccagctgc tgaacagcga ggatggcctg
     2821 ttcgtgtgca gaaaggatgc cgcctgtcag aggcctcacg aggactgcga aaattctgcc
     2881 ggcgaggaag aggacgaaga ggaagagaca atggacagcg agacagccaa gatggcttgc
     2941 cccagggacc agatgctgcc tgagcctatc tctttcgagg ccgctgccat tcctgtggcc
     3001 gagaaagaag aagccctgct gccagagcca gacgtgccca ccgatacaaa agagagcagc
     3061 gagaaggacg ccctgacaca gtaccccaga tacaagaagt accagctggc ctgcaccaag
     3121 aatgtgtaca acgccagcag ccacagcacc agcggctttg cctctacctt cagagaggac
     3181 aacagcagca acagcctgaa gcctggactg gccagaggcc agatcaagtc tgagcctcct
     3241 agcgaggaaa atgaagagga atctatcacc ctgtgcctga gcggcgacga gcctgatgcc
     3301 aaagatagag ctggcgacgt ggaaatggac cggaagcagc cttctccagc tcctacacct
     3361 acagctccag ctggcgcagc ctgcctggaa agatcaagat ctgtggctag ccccagctgc
     3421 ctgcggagcc tgtttagcat caccaagagc gtggaactga gcggcctgcc tagcacatcc
     3481 cagcagcact ttgccagatc tcccgcctgt cctttcgaca agggaatcac ccagggcgac
     3541 ctgaaaaccg actacacccc tttcaccggc aactacggac agcctcacgt gggacagaaa
     3601 gaggtgtcca actttacaat gggcagccct ctgagaggcc ctggccttga agccctgtgt
     3661 aaacaagagg gcgagctgga tcggcggagc gtgatctttt ctagcagcgc ctgtgaccag
     3721 gtgtccacca gcgtgcactc ttacagcgga gtgtccagcc tggataagga cctgtctgag
     3781 cccgtgccta aaggcctgtg ggttggagct ggacagagcc tgccaagcag ccaggcttat
     3841 tctcacggcg gactgatggc cgatcatctg cctggtagaa tgcggcccaa caccagctgt
     3901 cccgtgccaa tcaaagtgtg ccctagaagc cctcctctgg aaacccggac cagaaccagc
     3961 agcagctgtt ccagctacag ctatgccgag gatggaagcg gcggcagccc ttgttcactg
     4021 cctctgtgcg agtttagcag cagcccctgt tctcagggcg ccagatttct ggccaccgag
     4081 catcaagaac ctggcctgat gggcgacggc atgtacaatc aagtgcggcc ccagattaag
     4141 tgcgagcaga gctacggcac caacagctct gatgagagcg gcagctttag cgaggccgat
     4201 agcgaaagct gccccgtgca ggatagaggc caagaagtga agctgccctt tccagtggat
     4261 cagatcaccg acctgcctcg gaacgacttc cagatgatga tcaagatgca caagctgacc
     4321 tccgagcagc tggaattcat ccacgacgtg cggcggagaa gcaagaacag aatcgctgcc
     4381 cagcggtgcc ggaagagaaa gctggactgc atccagaatc tggaatgcga gatccggaag
     4441 ctcgtgtgcg aaaaagagaa gctgctgtcc gagcggaacc agctgaaggc ctgtatggga
     4501 gagctgctgg acaacttcag ctgtctgtct caagaagtgt gccgggacat ccagtctcca
     4561 gagcagattc aggccctgca cagatactgc cctgtgctga ggcctatgga tctgcctaca
     4621 gccagcagca tcaaccctgc tcctctggga gccgagcaga atattgccgc ctctcagtgt
     4681 gccgtgggcg agaatgtgcc ttgctgtctt gaacctggcg ccgctcctcc tggacctcct
     4741 tgggctcctt ctaacaccag cgagaactgc acctccggca gaaggctgga aggcacagat
     4801 cctggcacct tctctgagag aggcccacca ctggaaccca gatctcagac cgtgaccgtg
     4861 gacttctgcc aagagatgac cgacaagtgc accaccgacg agcagcccag aaaggactat
     4921 acatgataag gatcccccgg gctgcaggaa ttcgagcatc ttaccgccat ttattcccat
     4981 atttgttctg tttttcttga tttgggtata catttaaatg ttaataaaac aaaatggtgg
     5041 ggcaatcatt tacattttta gggatatgta attactagtt caggtgtatt gccacaagac
     5101 aaacatgtta agaaactttc ccgttattta cgctctgttc ctgttaatca acctctggat
     5161 tacaaaattt gtgaaagatt gactgatatt cttaactatg ttgctccttt tacgctgtgt
     5221 ggatatgctg ctttaatgcc tctgtatcat gctattgctt cccgtacggc tttcgttttc
     5281 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtccgt
     5341 caacgtggcg tggtgtgctc tgtgtttgct gacgcaaccc ccactggctg gggcattgcc
     5401 accacctgtc aactcctttc tgggactttc gctttccccc tcccgatcgc cacggcagaa
     5461 ctcatcgccg cctgccttgc ccgctgctgg acaggggcta ggttgctggg cactgataat
     5521 tccgtggtgt tgtcggggaa gctgacgtcc tttgcaattc gatatcaagc tgtaccttct
     5581 agatgtacat taggattagc gcttctagac caatgactta caaggcagct gtagatctta
     5641 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     5701 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     5761 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     5821 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     5881 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     5941 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     6001 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6061 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     6121 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6181 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     6241 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     6301 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     6361 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     6421 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     6481 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6541 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6601 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6661 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6721 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6781 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6841 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6901 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     6961 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     7021 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     7081 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     7141 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     7201 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     7261 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     7321 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     7381 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     7441 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     7501 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     7561 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     7621 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     7681 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7741 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7801 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7861 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7921 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7981 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     8041 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     8101 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8161 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     8221 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     8281 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     8341 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     8401 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8461 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8521 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8581 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8641 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8701 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8761 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8821 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     8881 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     8941 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     9001 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     9061 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     9121 gggaacaaaa gctggagctg ca
//
