LOCUS       sequence-450684-        7759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor FOS with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7759)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 7759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-FOS-Brd 185
FEATURES             Location/Qualifiers
     source          1..7759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1809..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1960..2366
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    3763..4166
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             4285..4518
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4590..4711
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4627..4646)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4681..4700
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4751..4886
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4906..4925)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4907..4925)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4935..4952)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4935..4951)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4944..4966)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5093..5548
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5180..5199)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5390..5411
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5574..5678
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5679..5747,5748..6539)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5897..5916)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6710..7298
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7199..7218
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7452..7469
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7586..7607
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7622..7627,7628..7645,7646..7652)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7660..7676
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7665..7687
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7684..7700
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7684..7700
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7719..7739
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7721..7739
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1921 aattttatcg atcacgagac tagcctcgag ctggctgcag taacgccatt ttgcaaggca
     1981 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2041 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2101 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2161 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2221 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2281 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2341 gtcctccgac agactgagtc ggccggtcga atcaagctta tcgataccgt cgacgccacc
     2401 atgatgttca gcggcttcaa cgccgactac gaggccagca gcagcagatg ttcttctgcc
     2461 tctcctgccg gcgacagcct gagctactat cactctcccg ccgacagctt cagcagcatg
     2521 ggatctccag tgaacgccca ggacttctgc acagatctgg ccgtgtccag cgccaacttc
     2581 atccctacag tgaccgccat cagcacaagc cctgacctgc aatggctggt gcagcctgct
     2641 ctggtgtcat ctgtggcccc tagccagaca agagcccctc atccttttgg cgtgccagct
     2701 ccttctgccg gcgcttattc tagagccggc gtggtcaaga ccatgacagg tggcagagcc
     2761 cagagcattg gcagacgggg aaaagtggaa cagctgagcc ccgaggaaga ggaaaagcgg
     2821 cggatcagac gcgagcggaa caaaatggcc gctgccaagt gcagaaaccg gcggagagag
     2881 ctgaccgata cactccaggc cgagacagac cagctggaag atgagaagtc tgccctccag
     2941 accgagatcg ccaacctgct gaaagagaaa gagaagctcg agttcatcct ggccgctcac
     3001 agacccgcct gcaagatccc tgatgatctg ggcttccccg aagagatgag cgtggcctct
     3061 ttggatctga caggcggact gcctgaagtg gccacacctg agagcgagga agccttcaca
     3121 ctgcccctgc tgaacgaccc tgagcctaag ccttctgtgg aacccgtgaa gtccatctcc
     3181 agcatggaac tgaaaaccga gcctttcgac gactttctgt tccccgccag ctctagacct
     3241 agcggctctg agacagctag aagcgtgccc gacatggatc tgagcggcag cttttatgcc
     3301 gccgactggg aacctctgca cagcggatct cttggcatgg gccctatggc cacagagctg
     3361 gaaccactgt gtacccctgt cgtgacctgc acacctagct gtaccgccta caccagcagc
     3421 ttcgtgttca cataccccga ggccgatagc ttcccttctt gtgccgccgc tcatagaaag
     3481 ggcagcagct ccaatgagcc cagcagcgat tctctgagca gccctacact gctggccctg
     3541 taataaggat cccccgggct gcaggaattc gagcatctta ccgccattta ttcccatatt
     3601 tgttctgttt ttcttgattt gggtatacat ttaaatgtta ataaaacaaa atggtggggc
     3661 aatcatttac atttttaggg atatgtaatt actagttcag gtgtattgcc acaagacaaa
     3721 catgttaaga aactttcccg ttatttacgc tctgttcctg ttaatcaacc tctggattac
     3781 aaaatttgtg aaagattgac tgatattctt aactatgttg ctccttttac gctgtgtgga
     3841 tatgctgctt taatgcctct gtatcatgct attgcttccc gtacggcttt cgttttctcc
     3901 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtccgtcaa
     3961 cgtggcgtgg tgtgctctgt gtttgctgac gcaaccccca ctggctgggg cattgccacc
     4021 acctgtcaac tcctttctgg gactttcgct ttccccctcc cgatcgccac ggcagaactc
     4081 atcgccgcct gccttgcccg ctgctggaca ggggctaggt tgctgggcac tgataattcc
     4141 gtggtgttgt cggggaagct gacgtccttt gcaattcgat atcaagctgt accttctaga
     4201 tgtacagatt tctcagcgct tctagaccaa tgacttacaa ggcagctgta gatcttagcc
     4261 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     4321 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     4381 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     4441 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     4501 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     4561 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     4621 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4681 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     4741 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4801 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4861 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     4921 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4981 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     5041 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     5101 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5161 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5221 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5281 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5341 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5401 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5461 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5521 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     5581 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5641 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5701 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5761 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5821 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5881 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5941 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6001 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6061 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6121 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6181 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6241 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6301 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6361 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6421 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6481 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6541 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6601 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6661 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6721 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6781 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6841 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6901 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6961 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7021 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7081 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7141 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7201 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7261 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7321 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7381 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7441 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7501 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     7561 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     7621 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     7681 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     7741 aacaaaagct ggagctgca
//
