LOCUS       sequence-450685-       10886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-terminally His6, Xpress and HA-tagged BIG1(WT) in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Shin HW, Morinaga N, Noda M, Nakayama K
  TITLE     BIG2, a guanine nucleotide exchange factor for ADP-ribosylation 
            factors: its localization to recycling endosomes and implication in 
            the endosome integrity.
  JOURNAL   Mol Biol Cell. 2004 Dec;15(12):5283-94. Epub 2004 Sep 22.
  PUBMED    15385626
REFERENCE   2  (bases 1 to 10886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2004-12"; volume: "15(12)"; pages: "5283-94. Epub 
            2004 Sep 22"
COMMENT     Sequence Label: pcDNA4c/His-Xpress-HA-hBIG1(WT)
FEATURES             Location/Qualifiers
     source          1..10886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(109..129)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        114..443
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      294..429
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     356..375
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        491..538
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             557..931
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1061..1182
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1098..1117)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1152..1171
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1231..1247)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1231..1247)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1244..1266)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1255..1271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1279..1285,1286..1303,1304..1309))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1324..1345
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1462..1479)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1633..2221)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1713..1732)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2392..3183,3184..3252))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3015..3034
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3253..3357)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3432..3451)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3623..4002
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4003..4206
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4157..4177
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4251..4270
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4251..4269
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    4305..4467
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             4480..4497
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4501..4533
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(4537..4545,4546..4560)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             4573..4599
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(10221..10237)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10221..10237)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(10282..10299)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10288..10512
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10558..10886,1..100)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10645..10664)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10855..10876
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
       61 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
      121 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
      181 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
      241 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
      301 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
      361 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
      421 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
      481 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
      541 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
      601 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
      661 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
      721 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
      781 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
      841 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
      901 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
      961 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     1021 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     1081 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     1141 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     1201 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     1261 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     1321 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     1381 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     1441 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1501 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1561 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1621 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1681 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1741 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1801 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1861 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1921 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1981 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2041 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2101 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2161 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2221 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2281 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2341 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2401 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2461 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2521 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2581 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2641 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2701 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2761 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     2821 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     2881 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     2941 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3001 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3061 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3121 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3181 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3241 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3301 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     3361 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     3421 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     3481 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     3541 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     3601 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     3661 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     3721 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     3781 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     3841 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     3901 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     3961 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     4021 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     4081 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     4141 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     4201 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
     4261 cactataggg agacccaagc tggctagcgt ttaaacttaa gcttagcgca gaggcttggg
     4321 gcagccgagc ggcagccagg ccccggcccg ggcctcggtt ccagaaggga gaggagcccg
     4381 ccaaggcgcg caagagagcg ggctgcctcg cagtccgagc cggagaggga gcgcgagccg
     4441 cgccggcccc ggacggcctc cgaaaccatg gggggttctc atcatcatca tcatcatggt
     4501 atggctagca tgactggtgg acagcaaatg ggtcgggatc tgtacgacga tgacgataag
     4561 gtacctacca tgtacccgta cgacgtgccc gactacgcgg gatccaccat gtatgagggg
     4621 aagaagacga agaacatgtt cctgacccgg gctctggaga agatattggc cgacaaggaa
     4681 gtgaagaagg cgcatcactc ccagctgcgc aaagcttgcg aggtggcgtt agaggaaata
     4741 aaagcggaaa ctgaaaaaca gagtcctcct catggagaag caaaagctgg atcaagcacc
     4801 cttccaccag tgaaatcaaa gacaaatttt attgaagcag acaagtactt cttgcctttt
     4861 gagttggcat gccagtccaa atgtcctcgc atagttagta catctctaga ttgcttacag
     4921 aaacttattg cttatgggca cttgactggc aatgctccag atagtacaac accaggcaaa
     4981 aaattaattg atagaattat tgaaacaata tgtggctgct ttcagggccc tcagacagat
     5041 gaaggagttc agctgcagat aataaaggct ttacttactg cagtaacatc acaacacata
     5101 gaaattcatg aagggactgt actgcaagct gtgagaacat gttacaatat ctacttagca
     5161 agcaaaaatc tcatcaatca gacaacagcc aaagctactc tcactcagat gctaaatgtt
     5221 atctttgcac gcatggaaaa ccaagcatta caggaagcaa aacaaatgga aaaagaaagg
     5281 catcggcagc atcatcatct gttacagtct ccagtaagcc atcacgagcc tgaatcacct
     5341 caacttagat atttgccacc tcagactgtt gatcatatat cccaagaaca cgaaggggac
     5401 cttgacctcc atacaaatga tgtagataaa agtcttcagg atgacacaga gcctgaaaat
     5461 ggatctgata tttccagtgc agaaaatgaa cagactgaag ctgatcaggc aactgcagct
     5521 gaaacattat ctaaaaatga agtgttatat gacggagaaa accatgattg tgaggaaaag
     5581 ccacaagaca ttgtacagaa cattgtagaa gaaatggtga acattgttgt tggagatatg
     5641 ggagaaggga ctactataaa tgcaagtgca gatggcaaca ttggaactat agaggatggt
     5701 agtgacagtg aaaatattca agcaaatgga attccaggaa caccaatttc tgttgcatat
     5761 acaccatcct tacctgatga tagattgtca gtctcttcca atgatactca ggaatctgga
     5821 aattcttcag gaccttcacc tggtgctaag ttttcccaca ttttacaaaa ggatgccttt
     5881 ctagtattca ggtcattgtg taaactgtca atgaaaccac tgtcagatgg accaccagat
     5941 ccaaagtctc atgaactacg atccaagatt ctttcattgc agttacttct atccattctg
     6001 cagaatgcag gacctatttt caggacaaat gagatgttta ttaatgctat taagcagtat
     6061 ctttgtgttg cactctcaaa aaatggagtc tcatctgttc cagaggtttt tgagctttct
     6121 ctttctatat ttcttacttt gttgtcaaat ttcaagacac atctgaagat gcaaattgag
     6181 gtgttcttta aagaaatttt cttatacatt ttggaaactt ctaccagctc atttgatcac
     6241 aaatggatgg ttattcagac actgacgagg atttgtgcag atgctcagag tgtagtggat
     6301 atttatgtaa actatgactg tgacttaaat gcagccaata tatttgaaag actagtaaat
     6361 gatctatcaa aaattgctca aggaaggggc agtcaagaac ttggtatgag taatgttcag
     6421 gaattgagcc tgaggaaaaa aggtttagaa tgcttagtgt cgattttgaa gtgtatggtt
     6481 gaatggagta aggatcagta tgtgaatccc aactcccaga caactcttgg tcaggaaaaa
     6541 ccctcagagc aagagatgag tgaaatcaaa caccctgaga caataaacag atacggaagt
     6601 ttaaattccc tggagtcaac atcatcatca ggaataggca gctacagtac acagatgtct
     6661 ggcactgata atccagaaca atttgaggtc ctaaagcaac aaaaagaaat aatagaacaa
     6721 gggatagatt tatttaataa gaaaccaaag agaggaatac agtacctcca agaacaaggg
     6781 atgcttggca ccacacctga agatattgcc caattcttac atcaagagga aagattagac
     6841 tctactcaag tgggtgagtt cctgggagat aatgataaat ttaacaaaga agtcatgtat
     6901 gcatatgtgg accaacatga cttttcagga aaagacttcg tttcagccct tcgtatgttt
     6961 ctagaaggat ttcgtcttcc aggggaagct cagaaaatcg atcgattaat ggaaaaattt
     7021 gctgcaagat acctagaatg caaccaagga caaactctct ttgctagtgc ggatacagct
     7081 tatgttttgg cttattcaat tatcatgttg accacagacc ttcacagtcc acaggtgaaa
     7141 aataaaatga caaaggaaca atacattaag atgaatagag gtatcaatga cagtaaagac
     7201 cttcctgaag agtatctatc agccatctat aatgaaatag ctgggaaaaa gatatcaatg
     7261 aaagaaacaa aagaactaac aatccctaca aaatcaagta aacagaatgt agccagtgaa
     7321 aaacaaagaa gacttctgta taacttagaa atggagcaga tggccaagac agctaaagca
     7381 ctcatggagg ccgtgagcca tgttcaggca ccctttacaa gtgcaacaca tttggagcat
     7441 gtgaggccca tgtttaagtt ggcttggacg ccttttctgg ctgcattcag tgtgggtcta
     7501 caagattgtg atgatactga agtagcctct ctttgcctgg aaggtataag atgtgcaatc
     7561 agaattgcat gcattttcag cattcagctg gagagagatg catatgtcca ggcactagca
     7621 agatttacct tactcacagt gagttccggt attactgaaa tgaaacagaa gaacattgac
     7681 acaataaaaa cacttatcac agtggctcat acagatggaa attatttagg aaattcatgg
     7741 catgagattc tgaagtgcat cagtcagttg gagctggcac agcttatagg aactggagtg
     7801 aaacctcgat acatttctgg aacagtgcga ggcagagaag gatctcttac tggaacaaaa
     7861 gatcaggctc ctgatgaatt tgtgggttta gggctagttg gaggaaatgt ggactggaaa
     7921 cagatagcaa gtattcagga atccattgga gaaaccagtt ctcagagtgt tgttgttgct
     7981 gtagatagaa tattcacagg atctacaagg ctagatggaa atgccattgt ggattttgtc
     8041 cgttggctct gtgctgtgtc tatggatgaa ttactttcca cgacacaccc aagaatgttt
     8101 agtctacaaa aaatagtaga aatatcatat tacaacatgg gaagaataag attacagtgg
     8161 tctcgaattt gggaagttat tggagatcat tttaataagg ttgggtgtaa tcctaatgaa
     8221 gatgtagcta tttttgcagt agactccttg aggcagttgt caatgaagtt cttagagaaa
     8281 ggggagcttg ctaacttcag attccagaag gatttcttaa gaccttttga acatataatg
     8341 aaacggaaca ggtctccaac aattcgagat atggttgtac ggtgtatagc acagatggtt
     8401 aattctcaag ctgctaacat tcgatctgga tggaagaaca ttttctctgt atttcatcta
     8461 gctgcatctg atcaagatga aagcatagtg gaacttgcat tccaaacaac cgggcacatt
     8521 gtcacccttg tatttgaaaa acactttcca gcgaccattg attctttcca ggatgcagtg
     8581 aagtgtttgt ctgaatttgc gtgcaatgca gctttcccag acacaagtat ggaagcaatt
     8641 cgacttattc gccattgtgc aaaatatgtg tctgatagac ctcaggcttt caaggaatac
     8701 acaagcgatg atatgaacgt agcacctgaa gacagggtgt gggtgagagg atggttccca
     8761 attctctttg agttatcctg tatcatcaat agatgcaaat tagatgtaag aaccaggggt
     8821 ttaacagtaa tgtttgaaat aatgaaaaca tatggccaca cttatgagaa acactggtgg
     8881 caggatttat ttagaattgt tttcagaatc tttgacaata tgaaattgcc agaacaacag
     8941 acagagaaag ctgaatggat gacaacaact tgcaatcatg cactttatgc aatctgtgat
     9001 gtattcactc agtatttaga agtactcagt gatgtacttt tggatgacat ttttgctcag
     9061 ctctactggt gtgtgcagca agacaatgag cagttagcgc gatctggtac aaactgttta
     9121 gagaatgttg ttattctgaa tggtgaaaaa tttaccctag aaatctggga taaaacttgc
     9181 aactgcacac tggatatctt caaaaccaca atcccacatg cgctgttgac ctggcgaccc
     9241 aattctggag aaactgcccc cccacctcca tctcctgtaa gtgaaaagcc attggataca
     9301 atatcacaga agtctgtaga tattcatgat tctattcaac caaggtctgt ggataacaga
     9361 ccacaagcac cactggtttc tgcgtctgct gttaatgaag aagtcagcaa aattaaatct
     9421 acagcaaaat ttccagaaca aaaattgttt gctgccctgt tgattaaatg tgttgtgcag
     9481 ctggaactca tccagactat cgacaacatt gtcttcttcc cagccacaag taagaaagaa
     9541 gatgcagaaa acttagctgc agcacagaga gatgcggtgg actttgatgt tcgcgttgat
     9601 actcaagacc aaggaatgta ccgcttttta acatcacaac aactttttaa gctactggac
     9661 tgcttattag agtcacatag atttgcaaaa gcgtttaatt ccaacaacga acagaggact
     9721 gccctgtgga aagcaggatt caaaggcaaa tccaagccca accttctgaa gcaggagacc
     9781 agcagcctgg cctgtgggct gcgcattctc ttccggatgt acatggatga gagccgcgtt
     9841 agtgcctggg aggaggtcca gcagaggctt ttgaatgtcc gcagtgaagc actaagttac
     9901 ttcctcactc taacatcaga aagtcatcga gaagcctgga ctaacttact gcttttgttt
     9961 ctaactaaag ttctaaagat aagtgataat aggtttaaag ctcatgcatc attctactac
    10021 cctctcttat gtgaaattat gcaatttgac ttgattcctg aacttcgtgc tgttcttaga
    10081 agattttttc tgcgaatcgg agtagttttt cagatatcac aaccacctga acaggaactt
    10141 ggaataaaca agcaatgatg ggaacttaat atttttgttg gcatttacat ccctctgcat
    10201 cgaattcctg cagcccgggg gatccactag ttctagagcg gccgctcgag tctagagggc
    10261 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
    10321 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
    10381 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
    10441 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
    10501 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
    10561 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
    10621 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
    10681 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
    10741 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
    10801 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
    10861 tcttgttcca aactggaaca acactc
//
