LOCUS       sequence-450687-       11069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-terminally His6, Xpress and HA-tagged BIG2(WT) in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Shin HW, Morinaga N, Noda M, Nakayama K
  TITLE     BIG2, a guanine nucleotide exchange factor for ADP-ribosylation 
            factors: its localization to recycling endosomes and implication in 
            the endosome integrity.
  JOURNAL   Mol Biol Cell. 2004 Dec;15(12):5283-94. Epub 2004 Sep 22.
  PUBMED    15385626
REFERENCE   2  (bases 1 to 11069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2004-12"; volume: "15(12)"; pages: "5283-94. Epub 
            2004 Sep 22"
COMMENT     Sequence Label: pcDNA4c/His-Xpress-HA-hBIG2(WT)
FEATURES             Location/Qualifiers
     source          1..11069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    683..845
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             858..875
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             879..911
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(915..923,924..938)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             951..977
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6843..6860)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6849..7073
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7119..7547
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7206..7225)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7416..7437
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7556..7576)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7561..7890
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7741..7876
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7803..7822
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7938..7985
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8004..8378
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8508..8629
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8545..8564)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8599..8618
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8678..8694)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8678..8694)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8691..8713)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8702..8718
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8726..8732,8733..8750,8751..8756))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8771..8792
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8909..8926)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9080..9668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9160..9179)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9839..10630,10631..10699))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10462..10481
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10700..10804)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10879..10898)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttagcgcaga ggcttggggc agccgagcgg cagccaggcc
      721 ccggcccggg cctcggttcc agaagggaga ggagcccgcc aaggcgcgca agagagcggg
      781 ctgcctcgca gtccgagccg gagagggagc gcgagccgcg ccggccccgg acggcctccg
      841 aaaccatggg gggttctcat catcatcatc atcatggtat ggctagcatg actggtggac
      901 agcaaatggg tcgggatctg tacgacgatg acgataaggt acctaccatg tacccgtacg
      961 acgtgcccga ctacgcggga tccaccatgc aggagagcca gaccaagagc atgttcgtgt
     1021 cccgggccct ggagaagatc ctagccgaca aggaggtgaa gcggccccag cactcccagc
     1081 tgcgcagggc ctgccaggtg gcgctcgatg aaattaaagc agaaatagaa aagcagaggc
     1141 ttggcactgc tgcaccacca aaggcaaact tcattgaagc tgacaagtat tttcttccat
     1201 tcgagctagc ttgccagtcc aagtccccaa gggtagtcag cacatccctt gactgcttgc
     1261 agaaactcat cgcatacggg cacatcactg gcaacgcccc tgacagtgga gcccctggga
     1321 agcggctgat cgacagaatt gttgaaacca tttgcagttg ttttcagggc cctcagactg
     1381 atgaaggggt tcagttacaa ataattaagg ctcttctgac tgcagtgact tccccacaca
     1441 ttgaaattca tgagggtact atcctgcaga cagtgagaac atgttacaat atctatttgg
     1501 ccagcaaaaa tctcatcaat caaaccactg ccaaggctac ccttactcag atgctgaacg
     1561 tcattttcac ccgcatggaa aaccaagtgt tgcaggaggc cagagaactg gaaaaaccaa
     1621 tccagtcaaa accccagtcc cctgtgatcc aagctgcagc agtatcccca aagttcgttc
     1681 gtttgaagca cagtcaggca caaagcaaac caacaactcc cgaaaaaaca gatttaacca
     1741 acggtgaaca tgccaggagt gattctggaa aagtaagcac agaaaatgga gacgcaccca
     1801 gagaaagagg ctcatcactg tcagggactg atgacggagc ccaggaggtg gtgaaggaca
     1861 tcttggaaga tgtagtcaca tctgccatta aagcagcgga aaagcatggt ctgacagaac
     1921 ctgagagagt tctaggtgaa ctggagtgcc aggaatgtgc tattccccca ggagttgatg
     1981 aaaactcaca gaccaacggg atagccgatg acaggcagtc cttgtcgtca gcagataatc
     2041 tggaatcgga tgcacaagga catcaagtgg ctgccaggtt ctcccacgtt ctgcagaagg
     2101 atgccttcct tgtgttccgc tccctgtgca agctgtccat gaaacccctt ggtgaaggcc
     2161 ctccagaccc aaaatcccat gagctgcgtt ccaaggtggt ttccctgcag ctgctcctct
     2221 ctgtgttgca aaatgctggc cccgtattca ggactcacga gatgttcatc aatgcaatca
     2281 agcaatatct ctgtgtggcc ttgtccaaaa acggcgtctc ttcagtgcct gatgtctttg
     2341 agctctctct tgccattttt cttactcttc tttcaaactt taaaatgcac ttgaaaatgc
     2401 agatagaggt ctttttcaaa gagattttcc tgaacatttt agaaacatca acaagttctt
     2461 ttgagcacag gtggatggtc attcagactc tgacgaggat ctgtgcagat gcccagtgtg
     2521 ttgtggatat ttatgtcaac tacgactgtg atttaaatgc tgctaacatt tttgagcgcc
     2581 ttgtaaatga tttatccaaa attgctcagg gaagaagtgg acatgagctg ggaatgacac
     2641 ctctgcagga gctcagcctg aggaagaaag gcctggagtg cctcgtgtcc attctcaagt
     2701 gcatggtgga gtggagcaaa gacctgtatg tgaatcccaa ccaccagacc agcctcggtc
     2761 aggagaggct cacggatcag gaaatagggg atgggaaagg ccttgacatg gcaagacggt
     2821 gtagtgtgac gtccatggag tccacagtgt cctcggggac ccagacaact gttcaggatg
     2881 accctgagca atttgaggtc atcaagcaac aaaaagaaat cattgaacac ggcatcgagc
     2941 tgttcaacaa gaaacccaag agggggatcc agtttctcca ggagcagggc atgctgggaa
     3001 cgtcagttga agacatagcc caattcctgc accaggagga gcgcctggat tccacccaag
     3061 taggcgattt tctgggagat agcgcaaggt tcaacaagga ggtgatgtat gcctacgtgg
     3121 accaacttga cttctgtgaa aaagaatttg tctcagccct gcggacattc ctagaaggtt
     3181 tccgcctacc tggagaagcc caaaagattg accgattaat ggagaagttt gccgcaagat
     3241 acatagaatg caaccaaggg caaactctgt ttgctagtgc tgacactgct tatgtcctag
     3301 cgtattcaat tattatgctg actacagact tgcacagtcc tcaggtaaaa aacaaaatga
     3361 cgaaagagca gtatattaaa atgaatcggg gtatcaatga tagtaaagat ctgccagaag
     3421 agtatctctc aagcatctat gaagagatag aaggcaagaa aattgcaatg aaagaaacaa
     3481 aagagctaac gattgcaacc aaatctacta agcagaatgt agctagtgaa aagcagcggc
     3541 ggctgctgta caacttagag atggagcaaa tggctaaaac agccaaagct ctgatggagg
     3601 ctgtgagcca tgccaaagcc ccgtttacca gtgccactca cctggaccat gtccggccaa
     3661 tgttcaaact ggtgtggacg ccactattgg cagcctacag catcggactc cagaactgtg
     3721 atgacactga agtggcctcc ttgtgtttgg aaggcatccg atgtgcaatc cgaatcgcct
     3781 gcatctttgg aatgcagctg gaacgagatg cctatgttca ggctcttgct cgcttctccc
     3841 tactcacagc cagctccagc atcacagaaa tgaagcagaa aaacatcgac accattaaga
     3901 cgcttatcac agtggctcac accgatggca actaccttgg gaattcctgg catgagatct
     3961 tgaaatgcat cagccagctg gagctcgctc agctgatagg aaccggtgtg aagacgcgct
     4021 acctgtctgg atctgggcgt gaaagagaag ggagcctgaa gggccacaca ttggcaggag
     4081 aagagttcat gggccttggc ctcggtaatt tggtgagtgg cggagtggat aaaagacaga
     4141 tggccagctt ccaagaatcg gttggtgaga ccagctcgca gagtgtggtt gtagctgtgg
     4201 acaggatttt tactgggtct accagactgg atggaaatgc aatagttgac tttgtccgct
     4261 ggctgtgtgc tgtgtccatg gatgaactgg cttcccccca ccatcctcgc atgttcagct
     4321 tgcagaagat tgtggagata tcatactaca acatgaatcg gatccgacta cagtggtctc
     4381 gaatatggca tgtgattgga gatcacttca ataaggttgg ctgcaaccct aatgaagatg
     4441 tggctatctt tgctgttgac tcattaaggc aactctccat gaagtttctt gagaagggtg
     4501 aattagccaa cttccgtttc cagaaagatt ttctgaggcc ctttgagcat attatgaaga
     4561 aaaacaggtc tcccaccatc cgggacatgg cgatccgctg cattgcccag atggtgaact
     4621 cccaggcggc caacatccgc tcaggctgga agaacatctt tgccgtgttc caccaggcag
     4681 cctctgatca tgatgggaac attgtggagc tggccttcca gaccacttgc cacattgtca
     4741 caactatttt ccagcaccat tttcctgcag ccatcgattc ctttcaggat gctgtgaagt
     4801 gcttatcaga gttcgcctgc aacgccgctt tccctgacac gagcatggaa gcgattcggc
     4861 tcatccgctt ctgtggcaaa tacgtctctg agaggcctcg ggtgctacaa gaatacacaa
     4921 gtgatgacat gaatgtagct cctggtgaca gagtctgggt ccgaggctgg ttccccatct
     4981 tattcgaact ctcctgcatc attaatagat gcaagttaga tgtacgaaca aggggactca
     5041 cagtcatgtt tgagatcatg aagagctatg gccacacctt tgaaaagcac tggtggcagg
     5101 acctgttcag aatcgtgttt cggatttttg acaatatgaa actccctgag caactgtcag
     5161 agaaatctga gtggatgaca acaacctgca atcacgcact ttatgctatt tgtgatgttt
     5221 ttacccagtt ttatgaagct ttgaatgaag ttcttctttc tgatgtattt gcacaattgc
     5281 agtggtgtgt caaacaagat aatgaacagt tggcgcgatc aggtacaaat tgcttagaaa
     5341 acttagtaat atccaatgga gagaaattca gtcctgaagt ctgggatgaa acctgcaact
     5401 gtatgttgga tattttcaaa acaaccatcc cacatgtttt gctgacatgg agacctgtag
     5461 gaatggagga agattcatca gaaaagcatt tggatgtgga tctggaccgc cagtctttaa
     5521 gcagcataga taaaaatccc tctgagaggg gacagagcca gctctctaac ccaacagatg
     5581 acagctggaa gggtagacca tacgcaaatc agaaactgtt tgccagcctc ctcatcaagt
     5641 gtgtggtcca gttggaattg atacagacca ttgacaacat tgtgttctac cctgcgacga
     5701 gcaaaaagga ggatgcagag cacatggttg ccgcccagca agacacgctg gatgcagata
     5761 tccacataga gacggaggat cagggcatgt ataagtacat gtcttcccag cacctcttca
     5821 agctgttgga ctgtttgcag gaatcccatt cattctcaaa ggccttcaac tccaattacg
     5881 agcagcggac tgtcctgtgg cgagcaggtt ttaagggcaa gtctaaaccc aatcttctaa
     5941 aacaagaaac cagcagcctg gcctgttgtt tgaggatcct gtttcgaatg tatgttgatg
     6001 agaaccgcag ggattcctgg gaagaaatac agcagagact tttaactgtt tgcagtgaag
     6061 ctcttgccta tttcatcact gtgaattctg agagccatcg ggaggcctgg acaagtctct
     6121 tgttgttact tctaactaaa acccttaaga taaatgatga aaagttcaaa gcacatgctt
     6181 caatgtacta cccctacttg tgtgaaatta tgcagtttga cctgatccct gagctccgag
     6241 cagttctgcg gaagttcttc ctacggatag gtgttgtgta taagatatgg ataccagaag
     6301 agccatcaca ggtaccagca gcactgtcac cagtgtggta gccctggctg cccaggccag
     6361 tgctgcagct ctgcagaatg ttcagcatgc catttctgac tggcacatct cgtgaagttt
     6421 catagaaaca aggagttggc atcttggatc tcagaatggc ctggaaacgg atggcctcta
     6481 cgctgttcca tcacagtctc caactaaggc ttatggtatt tcattaaact gttgcatacc
     6541 cagttagcac agtaggtggg gagtctgctt catttctatc attccatttt tctgattaaa
     6601 ctgtcaaatc tgtcattgca tatgccatcg ttttctagca aaatcccatg attggctata
     6661 aacgttttgt aagaagtcac tctccttgaa aatactgaac atagctgtat aggtttgtga
     6721 ttattagaga atatgttaat aaaacttctt gtaacggcta actgccacct aaaataaaaa
     6781 aaaaaaaaaa aaacgtttgc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc
     6841 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     6901 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     6961 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     7021 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     7081 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     7141 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     7201 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     7261 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     7321 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     7381 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     7441 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     7501 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     7561 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7621 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     7681 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     7741 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7801 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     7861 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     7921 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     7981 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
     8041 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     8101 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     8161 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     8221 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     8281 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     8341 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
     8401 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     8461 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     8521 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     8581 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     8641 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     8701 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     8761 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     8821 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     8881 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8941 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     9001 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     9061 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     9121 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     9181 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     9241 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     9301 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     9361 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     9421 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9481 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     9541 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9601 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9661 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9721 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9781 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9841 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9901 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9961 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    10021 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    10081 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    10141 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    10201 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    10261 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    10321 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    10381 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    10441 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    10501 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    10561 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10621 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10681 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10741 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10801 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
    10861 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
    10921 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
    10981 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
    11041 tcgcgatgta cgggccagat atacgcgtt
//
