LOCUS       sequence-450695-       10867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses cytosolic high affinity ratiometric ATP sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Marvin JS, Kokotos AC, Kumar M, Pulido C, Tkachuk AN, Yao JS, Brown 
            TA, Ryan TA
  TITLE     iATPSnFR2: A high-dynamic-range fluorescent sensor for monitoring 
            intracellular ATP.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2314604121. doi: 
            10.1073/pnas.2314604121. Epub 2024 May 15.
  PUBMED    38748581
REFERENCE   2  (bases 1 to 10867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-05-21"; volume: "121(21):e2314604121. 
            doi"; pages: " 10.1073/pnas.2314604121. Epub 2024 May 15"
COMMENT     Sequence Label: 
            plenti.CamKII.(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3
FEATURES             Location/Qualifiers
     source          1..10867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(48..67)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        239..618
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        620..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     773..793
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             836..1016
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1063..1188
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1681..1914
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2099..2143
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2441..2558
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2603..3891
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     primer_bind     4446..4467
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4570..4591)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    5560..6148
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5613..5633)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6031..6042)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6151..6167)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6152..6168)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6673..6853
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6879..6896)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6885..7109
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7155..7583
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7242..7261)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7452..7473
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7592..7612)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7597..7926
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7777..7912
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7839..7858
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7974..8021
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8040..8414
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8544..8665
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8581..8600)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8635..8654
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8714..8730)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8714..8730)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8727..8749)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8738..8754
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8762..8768,8769..8786,8787..8792))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8807..8828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8945..8962)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9116..9704)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9196..9215)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9875..10666,10667..10735))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10498..10517
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10736..10840)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
       61 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
      121 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
      181 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
      241 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      301 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      361 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      421 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      481 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      541 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      601 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      661 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      721 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      781 ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc
      841 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      901 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      961 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     1021 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     1081 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     1141 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     1201 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     1261 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     1321 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     1381 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     1441 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     1501 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     1561 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1621 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1681 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1741 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1801 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1861 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1921 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1981 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     2041 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     2101 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     2161 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     2221 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     2281 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     2341 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
     2401 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     2461 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     2521 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     2581 gagatccagt ttggttaatt aacattatgg ccttaggtca cttcatctcc atggggttct
     2641 tcttctgatt ttctagaaaa tgagatgggg gtgcagagag cttcctcagt gacctgccca
     2701 gggtcacatc agaaatgtca gagctagaac ttgaactcag attactaatc ttaaattcca
     2761 tgccttgggg gcatgcaagt acgatataca gaaggagtga actcattagg gcagatgacc
     2821 aatgagttta ggaaagaaga gtccagggca gggtacatct acaccacccg cccagccctg
     2881 ggtgagtcca gccacgttca cctcattata gttgcctctc tccagtccta ccttgacggg
     2941 aagcacaagc agaaactggg acaggagccc caggagacca aatcttcatg gtccctctgg
     3001 gaggatgggt ggggagagct gtggcagagg cctcaggagg ggccctgctg ctcagtggtg
     3061 acagataggg gtgagaaagc agacagagtc attccgtcag cattctgggt ctgtttggta
     3121 cttcttctca cgctaaggtg gcggtgtgat atgcacaatg gctaaaaagc agggagagct
     3181 ggaaagaaac aaggacagag acagaggcca agtcaaccag accaattccc agaggaagca
     3241 aagaaaccat tacagagact acaaggggga agggaaggag agatgaatta gcttcccctg
     3301 taaaccttag aacccagctg ttgccagggc aacggggcaa tacctgtctc ttcagaggag
     3361 atgaagttgc cagggtaact acatcctgtc tttctcaagg accatcccag aatgtggcac
     3421 ccactagccg ttaccatagc aactgcctct ttgccccact taatcccatc ccgtctgtta
     3481 aaagggccct atagttggag gtgggggagg taggaagagc gatgatcact tgtggactaa
     3541 gtttgttcgc atccccttct ccaaccccct cagtacatca ccctggggga acagggtcca
     3601 cttgctcctg ggcccacaca gtcctgcagt attgtgtata taaggccagg gcaaagagga
     3661 gcaggtttta aagtgaaagg caggcaggtg ttggggaggc agttaccggg gcaacgggaa
     3721 cagggcgttt cggaggtggt tgccatgggg acctggatgc tgacgaaggc tcgcgaggct
     3781 gtgagcagcc acagtgccct gctcagaagc cccaagctcg tcagtcaagc cggttctccg
     3841 tttgcactca ggagcacggg caggcgagtg gcccctagtt ctgggggcag cgggggatcc
     3901 gccaccatga agactattca cgtgagtgtc gtaactcccg acgggcctgt atatgaggat
     3961 gacgttgaaa tggtgagcgt caaagcaaaa tggggcgagc tcggtattct cccaggccac
     4021 attcccttga aagctcccct agagatcagt gccgcacgcc tgaagaaagg gggcaaaaca
     4081 cagtatatcg ctgtgtcagg cggcaacctc gaagtgcggc ctgacaaggt gaccatctat
     4141 gcccaggcag ccgagagggc tgaggatatc gatgtcctgc gcgccaagaa agccaaggag
     4201 agagccgagc gccgactcca atcacaggtc ctggtcggga acgtctatat caccgccgac
     4261 aagcagaaga acggcatcaa ggcgaacttc aagatccgcc acaacgtgga ggacggcagc
     4321 atgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4381 cccgacaacc actacctgag cacccagtcc gtgctgagca aagaccctaa cgagaagcgc
     4441 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4501 ctgtacaagg gcggtaccgg agggagcatg agcaagggcg aggagctgtt caccggggtg
     4561 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgcgcggc
     4621 gagggcgagg gcgatgccac caacggcaag ctgaccctga agttcatctg caccaccggc
     4681 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     4741 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     4801 tacgtccagg agcgcaccat cagcttcaag gacgacggca cctacaagac ccgcgccgag
     4861 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     4921 gaggacggca acatcctggg gcacaagctg gagtacaact ttatttgtgt ggacatcgat
     4981 tttaagcgcg ccgagctcgc ccttaagcgc gcaatgaata ggctctcagt tgccgaaatg
     5041 aagctgcagg gcggaggctc gggcggaggt gggtcgggtg gcggcggatc aatggcaaac
     5101 ctggacaaga tgctgaacac caccgtgacc gaggtgcgca agttcctgca agcggacaga
     5161 gtgtgcgtgt tcaagttcga ggaagattac tccggcaccg tctcgcacga agccgtggac
     5221 gacagatgga ttagcatcct gaagacccag gtgcaggaca gatacttcat ggaaaccaga
     5281 ggcgaggaat acgtccacgg cagataccag gccatcgccg acatctacac agccaatctg
     5341 gtcgagtgct acagagacct gctgatcgag tttcaggtgc gggccattct ggctgtcccc
     5401 atcctgcaag gcaagaagct gtggggcctg ctggtggccc accaactggc cggccctcgg
     5461 gagtggcaga cctgggaaat cgacttcctg aaacagcaag ccgtggtgat gggcatcgcc
     5521 atccagcaga gctaggaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
     5581 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     5641 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     5701 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     5761 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     5821 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     5881 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     5941 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     6001 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6061 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     6121 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa
     6181 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     6241 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     6301 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     6361 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     6421 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     6481 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     6541 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     6601 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     6661 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6721 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6781 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6841 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     6901 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     6961 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     7021 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     7081 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     7141 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     7201 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     7261 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     7321 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7381 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7441 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7501 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     7561 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     7621 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7681 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7741 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7801 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7861 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     7921 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     7981 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     8041 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     8101 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     8161 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     8221 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     8281 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     8341 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     8401 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     8461 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     8521 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     8581 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8641 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     8701 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8761 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8821 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8881 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     8941 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     9001 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     9061 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     9121 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     9181 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     9241 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     9301 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9361 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9421 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9481 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9541 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9601 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9661 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9721 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9781 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9901 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9961 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    10021 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    10081 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    10141 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    10201 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    10261 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    10321 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    10381 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    10441 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    10501 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10561 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10621 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10681 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10741 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    10801 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    10861 cacctga
//
