LOCUS       sequence-450696-        2670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0 - promoter vector carrying promoter 
            MinSyn104.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Shakhova ES, Markina NM, Mitiouchkina T, Bugaeva EN, Karataeva TA, 
            Palkina KA, Fakhranurova LI, Yampolsky IV, Sarkisyan KS, Mishin AS
  TITLE     Systematic Comparison of Plant Promoters in Nicotiana spp. 
            Expression Systems.
  JOURNAL   Int J Mol Sci. 2022 Dec 6;23(23):15441. doi: 10.3390/ijms232315441.
  PUBMED    36499768
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2022-12-6"; volume: "23(23):15441. doi"; pages: " 
            10.3390/ijms232315441"
COMMENT     Sequence Label: pNK883
FEATURES             Location/Qualifiers
     source          1..2670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      170..758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     659..678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     912..929
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1466..1484)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1552..1656
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1878..2669
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
       61 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
      121 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
      181 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
      241 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
      301 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
      361 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
      421 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
      481 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
      541 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
      601 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
      661 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
      721 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
      781 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
      841 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
      901 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
      961 ctctccccgc gcgttggccg attcattaat cactctgtgg tctcaggagg cgtgtcgttt
     1021 tagtgaggat cgaaaggaca gtaactgcta ggaggaccga tgctgatctt ccatcaacaa
     1081 ataatccaag taaggtaggt cacgaccact ataatttcgg gaaacctcct cggctatcgc
     1141 tttagcaaga cctctacatt gcgataaagg aaaggctatg cccaattatg acccccgccg
     1201 atgacgcggg aaaaactggt acttgttgaa gcatcttcca ctatgcccaa tggtggagca
     1261 cgacaaaact ggtacttgtg tacagctgac gtaagggatg acgcacatat cagcttagca
     1321 agacctcttg aagataagat aataatgttg aagataagat gcctgccttg atgactatat
     1381 aaggttttgc tattcattga aagcagtagt gactgatttg tatatatact tgagaccacg
     1441 aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     1501 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     1561 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1621 tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa ctggtccaga
     1681 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1741 tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     1801 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     1861 aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag
     1921 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     1981 gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg gttacggtga
     2041 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     2101 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     2161 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     2221 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     2281 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     2341 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     2401 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     2461 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     2521 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     2581 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccat tacgtaaaag
     2641 gcgagatcac caaggtagtc ggcaaataac
//
